MCU-i4

Catalog No.S9842

For research use only.

MCU-i4 is a negative modulator of the mitochondrial calcium uniporter (MCU) complex that directly binds a specific cleft in MICU1 and decreases mitochondrial Ca2+ influx.

MCU-i4 Chemical Structure

CAS No. 371924-24-2

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Biological Activity

Description MCU-i4 is a negative modulator of the mitochondrial calcium uniporter (MCU) complex that directly binds a specific cleft in MICU1 and decreases mitochondrial Ca2+ influx.
Targets
MCU1 [1]
In vitro

MCU-i4 decreases mitochondrial Ca2+ influx. Docking simulations reveal that MCU-i4 directly binds a specific cleft in MICU1, a key element of the MCU complex that controls channel gating. Accordingly, in MICU1-silenced or deleted cells, the inhibitory effect of MCU-i4 is lost. Moreover, MCU-i4 fails to inhibit mitochondrial Ca2+ uptake in cells expressing a MICU1 mutated in the critical amino acids that forge the predicted binding cleft.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: C2C12 myoblasts
  • Concentrations: 10 μM
  • Incubation Time: 24 h
  • Method:

    Growing medium of 90% confluent C2C12 myoblasts is replaced with DMEM containing 2%-heat inactivated horse serum (HS) (differentiation medium). Five days later, cells are incubated for 24 h with MCU-i4 (10 μM) or with DMSO alone (0.1% v/v). At the end of the experiment, myotubes images are taken and myotubes width is measured by means of ImageJ software.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 377.48
Formula

C23H27N3O2

CAS No. 371924-24-2
Storage 3 years -20°C powder
2 years -80°C in solvent

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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