Indoximod (NLG-8189)

Catalog No.S7756 Synonyms: 1-Methyl-D-tryptophan, 1-MT

For research use only.

Indoximod (NLG-8189, 1-Methyl-D-tryptophan, 1-MT), a methylated tryptophan, acts as an IDO (indoleamine-(2,3)-dioxygenase) pathway inhibitor, and reverses IDO-mediated immune suppression. Phase 2.

Indoximod (NLG-8189) Chemical Structure

CAS No. 110117-83-4

Selleck's Indoximod (NLG-8189) has been cited by 7 Publications

Purity & Quality Control

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Biological Activity

Description Indoximod (NLG-8189, 1-Methyl-D-tryptophan, 1-MT), a methylated tryptophan, acts as an IDO (indoleamine-(2,3)-dioxygenase) pathway inhibitor, and reverses IDO-mediated immune suppression. Phase 2.
Targets
IDO [1]
In vitro

Indoximod significantly reverses the suppression of T cells created by IDO-expressing dendritic cells, using both human monocyte-derived dendritic cells and murine dendritic cells isolated directly from tumor-draining lymph nodes. [1] Indoximod activates a Trp sufficiency signal that stimulates mTOR, and relieves IDO-induced Trp deprivation as well as an autophagic response. [2]

In vivo In mouse models of transplantable melanoma and transplantable and autochthonous breast cancer, Indoximod (400 mg/kg p.o.) enhances antitumor immunity in the setting of combined chemo-immunotherapy regimens. [1]

Protocol (from reference)

Animal Research:[1]
  • Animal Models: Mouse B16F10 and 4T1 tumor models
  • Dosages: 400 mg/kg
  • Administration: p.o.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

0.1mg/mL

Chemical Information

Molecular Weight 218.25
Formula

C12H14N2O2

CAS No. 110117-83-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN1C=C(C2=CC=CC=C21)CC(C(=O)O)N

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03301636 Terminated Drug: Pembrolizumab|Drug: Nivolumab|Drug: Indoximod Melanoma NewLink Genetics Corporation|Lumos Pharma December 8 2017 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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