Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor Gemcitabine Hydrochloride

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Gemcitabine Hydrochloride DNA Synthesis inhibitor

Cat.No.S1149

Gemcitabine is a pyrimidine nucleoside analog antitumor drug that can inhibit DNA synthesis and repair, leading to cell autophagy and apoptosis. It is mainly used to treat various solid tumors such as pancreatic cancer and non-small cell lung cancer, and is often combined with chemotherapy to enhance efficacy.
Gemcitabine Hydrochloride DNA/RNA Synthesis inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 299.66
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Quality Control

Batch: Purity: 99.96%
99.96

Solubility in DMSO or Water

In vitro
Batch:

Water : 59 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

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Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO
%
% Tween 80
% ddH2O
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Read more about Gemcitabine Hydrochloride solubility in DMSO or water

Working Concentrations for Cell Culture Treatment

Step 1: Select a research area to find working concentrations, or search directly for a cell line

Read more about Gemcitabine Hydrochloride working concentrations for cell culture treatment

Working Concentrations for Animal Model Treatment

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Mechanism of Action

Information Gemcitabine Hydrochloride is a potent RRM2 and DHFR inhibitor. It affects cGAS-STING and PI3K/Akt/mTOR pathways by incorporating into DNA and depleting nucleotides, inhibiting DNA synthesis and tumor proliferation.

Read more about Gemcitabine Hydrochloride IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Read more about Gemcitabine Hydrochloride Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight 299.66 Formula

C9H11F2N3O4.HCI

 Storage (From the date of receipt)
CAS No. 122111-03-9 Download SDF Storage of Stock Solutions

Synonyms NSC 613327 HCl,LY-188011 HCl Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

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Read more comparative analysis about Gemcitabine Hydrochloride

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

Question 1:
What’s the difference between S1714 and S1149 and which one is better?

Answer:
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and it dissolves better in water.