Diflunisal

Catalog No.S4609 Synonyms: Dolobid, Dolobis, Flovacil, Fluniget

For research use only.

Diflunisal (Dolobid, Dolobis, Flovacil, Fluniget) is a difluorophenyl derivate of salicylic acid and a nonsteroidal anti-inflammatory drug (NSAID) with antipyretic, analgesic and anti-inflammatory properties. The mechanism of action of diflunisal is as a Cyclooxygenase Inhibitor.

Diflunisal Chemical Structure

CAS No. 22494-42-4

Purity & Quality Control

Choose Selective COX Inhibitors

Other COX Products

Biological Activity

Description Diflunisal (Dolobid, Dolobis, Flovacil, Fluniget) is a difluorophenyl derivate of salicylic acid and a nonsteroidal anti-inflammatory drug (NSAID) with antipyretic, analgesic and anti-inflammatory properties. The mechanism of action of diflunisal is as a Cyclooxygenase Inhibitor.
Targets
COX [1]
()
p300 [2]
996 μM
In vitro

Diflunisal, an FDA-approved drug containing a salicylic acid substructure, inhibited CBP/p300 more potently than salicylate. Diflunisal exhibits anti-tumor activity against a specific leukemia carrying a t(8;21) translocation, a tumor previously reported to be dependent on p300 in vitro and in vivo[2].

In vivo Diflunisal-mediated kinetic stabilization of TTR(Rate-limiting transthyretin) should ameliorate TTR amyloidoses, provided that the nonsteroidal anti-inflammatory drug liabilities can be managed clinically[3].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: HEK293T cells
  • Concentrations: 0, 100, 200, 400 μM
  • Incubation Time: 24 h
  • Method:

    HEK293T cells are transfected with expression vectors for WT p300 or catalitycally inactive mutant. 24 hr after transfection, cells are treated with diflunisal as indicated for 24 hr. H2B acetylation is measured by Western Blot using specified antibodies. Experiments are repeated four times.

Animal Research:

[1]

  • Animal Models: Female Sprague-Dawley rats, Female New Zealand White Rabbits
  • Dosages: 250 mg/kg
  • Administration: oral administration

Solubility (25°C)

In vitro

DMSO 50 mg/mL
(199.84 mM)
Water Insoluble
Ethanol '20 mg/mL

Chemical Information

Molecular Weight 250.20
Formula

C13H8F2O3

CAS No. 22494-42-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=C(C=C1C2=C(C=C(C=C2)F)F)C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Diflunisal | Diflunisal supplier | purchase Diflunisal | Diflunisal cost | Diflunisal manufacturer | order Diflunisal | Diflunisal distributor