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Biotin (Vitamin B7) Dyes inhibitor

Name: Biotin (Vitamin B7)
Brand: Selleck Chemicals
SKU: S3130
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S3130

Biotin (Vitamin B7) is a B-vitamin and acts as a necessary coenzyme for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. It is covalently attached to distinct lysine residues in histones, affecting chromatin structure and mediating gene regulation.

Chemical Structure

Molecular Weight: 244.31

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Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Dyes Inhibitors	Fluorofurimazine (FFz) CFSE (CFDA-SE) Propidium Iodide DFO Thiazolyl Blue Hoechst 33342 D-Luciferin Crystal Violet D-Luciferin sodium salt Dihydroethidium

Solubility

In vitro
Batch:

DMSO : 49 mg/mL (200.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.450mg/ml (10.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 49 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.408mg/ml (1.67mM)	Taking the 1 mL working solution as an example, add 50 μL of 8.16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	244.31	Formula	C₁₀H₁₆N₂O₃S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	58-85-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			SMILES	C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro

Biotin (Vitamin B7) is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. It plays a role in the citric acid cycle, which is the process by which biochemical energy is generated during aerobic respiration. This compound is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. In addition, it is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays.

References

[1] https://pubmed.ncbi.nlm.nih.gov/15539280/
[2] https://pubmed.ncbi.nlm.nih.gov/10064317/
[3] https://pubmed.ncbi.nlm.nih.gov/16772434/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-05-06)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07302880	RECRUITING	Blood Sample; Interference With Routine Analyical Tests; Biotin Ingestion	University of Copenhagen	2026-03-17
NCT06313398	RECRUITING	Sickle Cell Disease; Thalassemia; Hemoglobinopathy	National Heart, Lung, and Blood Institute (NHLBI)	2024-05-17	EARLY_PHASE1
NCT07446647	RECRUITING	Healthy Volunteer Red Blood Cell Labeling Study; Not Disease Focused	Jose Cancelas	2026-04-01	PHASE1
NCT07513532	COMPLETED	Healthy Volunteers	Bloodworks	2026-04-06	PHASE1
NCT06740240	RECRUITING	Tumor	First Affiliated Hospital of Fujian Medical University	2024-03-01	EARLY_PHASE1
NCT07520019	ACTIVE_NOT_RECRUITING	Hair Thinning	Nutra Harmony LLC	2026-01-15

Tech Support

Handling Instructions

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