research use only

Thiazolyl Blue Dyes inhibitor

Name: Thiazolyl Blue
Brand: Selleck Chemicals
SKU: S6821
Availability: InStock
Rating: 5 (9 reviews)

Cat.No.S6821

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. This compound is reduced from yellow color to purple formazan in living cells.

Chemical Structure

Molecular Weight: 414.32

Jump to

Quality Control

Batch: S682101 DMSO]21 mg/mL]false]Ethanol]7 mg/mL]false]Water]2 mg/mL]false Purity: 99.86%

Cited in Nature Medicine for its top-tier quality
COA
HPLC
SDS
Datasheet

99.86

Related Targets	PROTAC Others Antineoplastic and Immunosuppressive Antibiotics ADC Cytotoxin Selection Antibiotics for Transfected Cell Antibiotics for Mammalian Cell Culture ADC Linker Molecular Glues Hydrotropic Agents Antibiotics for Plant Cell Culture
Other Dyes Inhibitors	Fluorofurimazine (FFz) CFSE Propidium Iodide DFO D-Luciferin Crystal Violet Hoechst 33342 D-Luciferin sodium salt Dihydroethidium WST-8

Solubility

In vitro
Batch:

DMSO : 21 mg/mL (50.68 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 7 mg/mL

Water : 2 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	414.32	Formula	C₁₈H₁₆BrN₅S	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
CAS No.	298-93-1	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium			Smiles	CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=CC=C3)C4=CC=CC=C4)C.[Br-]

Mechanism of Action

In vitro

1.Preparation of MTT working solution
Dissolve this compound by PBS to obtain 5 mg/mL of MTT.
2.Cell proliferation detection ( 96-well plates)
2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.
2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h.
2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.
2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely
2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

References

[1] https://pubmed.ncbi.nlm.nih.gov/16216776/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06364137	Not yet recruiting	Anxiety Disorders\|Mood Disorders	Stanford University\|National Institute of Mental Health (NIMH)\|MainSquare Co. (dba ''Teleo'')	July 1 2024	Not Applicable
NCT06376500	Not yet recruiting	Transcranial Direct Current Stimulation\|Post-Concussion Syndrome\|Functional Magnetic Resonance Imaging\|Magnetic Resonance Spectroscopy	The Hong Kong Polytechnic University	June 2024	Not Applicable
NCT05516472	Not yet recruiting	Kidney Stones	Joshua Stern\|University of Minnesota\|Albert Einstein College of Medicine\|Litholink Corporation\|Intermountain Health Care Inc.	April 2024	Early Phase 1
NCT06382480	Recruiting	Overweight\|Obesity\|Prediabetes\|Type 2 Diabetes	German Institute of Human Nutrition\|Charite University Berlin Germany\|Helmholtz Center Munich\|Hasso Plattner Institute Potsdam Germany	April 12 2024	Not Applicable
NCT06197308	Recruiting	Primary Sclerosing Cholangitis	University of Minnesota	March 1 2024	Early Phase 1

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):