Thiazolyl Blue

For research use only.

Catalog No.S6821 Synonyms: MTT, Thiazolyl Blue Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium, Tetrazolium Bromide

1 publication

Thiazolyl Blue Chemical Structure

CAS No. 298-93-1

Thiazolyl Blue (MTT) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

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Selleck's Thiazolyl Blue has been cited by 1 publication

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Biological Activity

Description Thiazolyl Blue (MTT) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

Protocol

Solubility (25°C)

In vitro DMSO 21 mg/mL (50.68 mM)
Ethanol 7 mg/mL (16.89 mM)
Water 2 mg/mL (4.82 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 414.32
Formula

C18H16BrN5S

CAS No. 298-93-1
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Synonyms MTT, Thiazolyl Blue Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium, Tetrazolium Bromide
Smiles CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=CC=C3)C4=CC=CC=C4)C.[Br-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04208477 Not yet recruiting Procedure: one site blockade of the stellate ganglion Traumatic Brain Injury|Brain Inflammation|Cerebral Vasospasm University Medical Centre Ljubljana August 15 2020 Not Applicable
NCT04283617 Not yet recruiting Behavioral: Intensification of insulin regimen/Nutrition Diabete Type 2 Yale University July 1 2020 Not Applicable
NCT04323566 Not yet recruiting Drug: Rituximab Obsessive-Compulsive Disorder|Obsessive-Compulsive Behavior|Schizophrenia|Delusional Disorder|Brief Psychotic Disorder|Schizo Affective Disorder|Other Psychoses|Unspecified Psychosis Uppsala University Hospital|Uppsala University May 1 2020 Phase 2
NCT04141371 Not yet recruiting Drug: Molar Sodium Lactate|Drug: physiological serum Subarachnoid Hemorrhage Aneurysmal Centre Hospitalier Universitaire de Nice January 1 2020 Phase 3
NCT04397640 Recruiting Diagnostic Test: Sonovue Sepsis|Microcirculation Universitair Ziekenhuis Brussel January 31 2020 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID