CFSE

For research use only.

Catalog No.S8269 Synonyms: Carboxyfluorescein succinimidyl ester

5 publications

CFSE Chemical Structure

CAS No. 150347-59-4

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.

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Biological Activity

Description Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.
In vitro

The binding of CFDA-SE fluorescent dye to cells is stable. It is not toxic and does not modify the viability of labeled cells. CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling. CFDA-SE persists in cells 24 h after labeling, allowing the long-term detection of labeled cells[1].

In vivo In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs[2].

Protocol

Cell Research:

[1]

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  • Cell lines: human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375
  • Concentrations: 2 µM, 3 µM, 4 µM, 5 µM, 10 µM and 20 µM
  • Incubation Time: 5, 6, 7, 8, 10 and 15 min
  • Method:

    The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 µM, 3 µM, 4 µM, 5 µM, 10 µM and 20 µM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: C57Bl/6 mice
  • Dosages: 10 μM
  • Administration: injected into thymic lobe
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (179.38 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 557.46
Formula

C29H19NO11

CAS No. 150347-59-4
Storage powder
in solvent
Synonyms Carboxyfluorescein succinimidyl ester
Smiles CC(=O)OC1=CC=C2C(=C1)OC3=C(C=CC(=C3)OC(C)=O)C24OC(=O)C5=CC(=CC=C45)C(=O)ON6C(=O)CCC6=O.CC(=O)OC7=CC=C8C(=C7)OC9=C(C=CC(=C9)OC(C)=O)C8%10OC(=O)C%11=CC=C(C=C%10%11)C(=O)ON%12C(=O)CCC%12=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02028403 Completed Drug: BMS-936559|Drug: Placebo for BMS-936559 HIV Infections National Institute of Allergy and Infectious Diseases (NIAID) June 2014 Phase 1
NCT01376726 Completed Biological: Sub C gp140 Vaccine|Biological: MF59C.1 Adjuvant HIV Infections National Institute of Allergy and Infectious Diseases (NIAID)|Novartis Vaccines and Diagnostics Inc July 2011 Phase 1
NCT01029548 Completed -- HIV Infections Barbara Ensoli MD|Istituto Superiore di Sanità April 2008 --
NCT01024556 Completed -- HIV Infection Barbara Ensoli MD|Istituto Superiore di Sanità March 2008 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID