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Propidium Iodide Dyes chemical

Name: Propidium Iodide
Brand: Selleck Chemicals
SKU: S6874
Availability: InStock
Rating: 5 (19 reviews)

Cat.No.S6874

Propidium iodide (PI) is a red-fluorescent DNA stain which penetrates only damaged cellular membranes. Propidium iodide (PI) is described for use in the determination of cell viability in cell suspension.

Chemical Structure

Molecular Weight: 668.39

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Quality Control

Batch: S687401 DMSO]100 mg/mL]false]Water]10 mg/mL]false]Ethanol]1 mg/mL]false Purity: 99.79%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS
Datasheet

99.79

Related Targets	PROTAC Others Antineoplastic and Immunosuppressive Antibiotics ADC Cytotoxin Selection Antibiotics for Transfected Cell Antibiotics for Mammalian Cell Culture ADC Linker Molecular Glues Antibiotics for Plant Cell Culture PROTAC Linker
Other Dyes Products	CFSE D-Luciferin D-Luciferin sodium salt Crystal Violet Hematoxylin DFO WST-8 Thiazolyl Blue Dihydroethidium Hoechst 33342

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (149.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 10 mg/mL

Ethanol : 1 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	668.39	Formula	C₂₇H₃₄N₄.₂I	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	25535-16-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	PI			Smiles	CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]

Mechanism of Action

References	[1] https://pubmed.ncbi.nlm.nih.gov/7579556/ [2] https://pubmed.ncbi.nlm.nih.gov/2578146/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01037387	Recruiting	Chronic Obstructive Pulmonary Disease\|Hypercapnic	Hospital Universitario La Paz	December 2025	Phase 4
NCT04860570	Recruiting	Acute Appendicitis\|Surgical Site Infection	Hospital Medina del Campo	October 25 2024	--
NCT06160271	Not yet recruiting	Non-alcoholic Steatohepatitis	Central Hospital Nancy France	September 1 2024	Phase 2
NCT05928078	Not yet recruiting	Cognitive Decline	University of Castilla-La Mancha	September 1 2024	Not Applicable

Tech Support

Handling Instructions

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