Home Others Dyes chemical Propidium Iodide For research use only.

Propidium Iodide

Synonyms: PI

Propidium iodide (PI) is a red-fluorescent DNA stain which penetrates only damaged cellular membranes. Propidium iodide (PI) is described for use in the determination of cell viability in cell suspension.

Propidium Iodide Chemical Structure

Propidium Iodide Chemical Structure

CAS: 25535-16-4

Selleck's Propidium Iodide has been cited by 11 publications

Purity & Quality Control

Batch: S687401 DMSO] 100 mg/mL] false] Water] 10 mg/mL] false] Ethanol] 1 mg/mL] false Purity: 99.79%
99.79

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Biological Activity

Description Propidium iodide (PI) is a red-fluorescent DNA stain which penetrates only damaged cellular membranes. Propidium iodide (PI) is described for use in the determination of cell viability in cell suspension.
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Chemical Information & Solubility

Molecular Weight 668.39 Formula

C27H34N4.2I
CAS No. 25535-16-4 SDF --
Smiles CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (149.61 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 10 mg/mL

Ethanol : 1 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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