D-Luciferin

Catalog No.S7763 Synonyms: Firefly luciferin

For research use only.

D-Luciferin (Firefly luciferin) is a popular bioluminescent substrate of luciferase in the presence of ATP, used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications.

D-Luciferin Chemical Structure

CAS No. 2591-17-5

Selleck's D-Luciferin has been cited by 5 Publications

Purity & Quality Control

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Biological Activity

Description D-Luciferin (Firefly luciferin) is a popular bioluminescent substrate of luciferase in the presence of ATP, used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications.
In vitro

D-luciferin reacts with luciferase, ATP and oxygen with light emission, and the light is detected by a sensitive photographic film, thereby permitting the visualization of the alkaline phosphatase-conjugated antibodies. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHO cells NH;IfGxHfW6ldHnvckBie3OjeR?= Mn20SIl{eGyjY3Xt[Y51KG:oIGuzTH1RW0JvMUOyOVMh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDHVHI{PSCneIDyd5Nm\CCrbjDDTG8h[2WubIOgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXNuIFvpQVkvQDZizszN NWP3O3JDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4PFg6OzJpPkKzPFg5QTN{PD;hQi=>
HT29 cells  M1vvPWZ2dmO2aX;uJIF{e2G7 NVvPU2Y2SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBIWFJ|NTDlfJBz\XO|ZXSgbY4hcHWvYX6gTHQzQSClZXzsd{BjgSCGTWKgZZN{[XluIFXDOVA:OTJwOTFOwG0> NXy2WWg1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4PFg6OzJpPkKzPFg5QTN{PD;hQi=>
In vivo In an immunocompetent mouse model of ovarian cancer, use of D-luciferin substrate and firefly luciferase preserves tumor-host immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. [2]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 280.32
Formula

C11H8N2O3S2

CAS No. 2591-17-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)O)C(=O)O

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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