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Dihydroethidium Dyes chemical

Name: Dihydroethidium
SKU: S6792
Availability: InStock
Rating: 5 (6 reviews)

Cat.No.S6792

Dihydroethidium (DHE, HE, Hydroethidine, PD-MY 003) is a cell-permeable blue fluorogenic probe used for detecting intracellular superoxide radical anion.

Chemical Structure

Molecular Weight: 315.41

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S679201 DMSO]63 mg/mL]false]Ethanol]2 mg/mL]false]Water]Insoluble]false Purity: 98.16%

98.16

Chemical Information, Storage & Stability

Molecular Weight	315.41	Formula	C₂₁H₂₁N₃	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
CAS No.	104821-25-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	DHE, HE, Hydroethidine, PD-MY 003			Smiles	CCN1C(C2=CC=CC=C2)C3=C(C=CC(=C3)N)C4=C1C=C(N)C=C4

Solubility

In vitro
Batch:

DMSO : 63 mg/mL (199.74 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro

1.1 Preparation of the stock solution
Dissolve 1 mg of Dihydroethidium in 0.31 mL of DMSO to obtain 10 mM of this compound.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of this chemical working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of this compound working solution.
Note: Please adjust the concentration of this chemical working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of this compound working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

References

[1] https://pubmed.ncbi.nlm.nih.gov/20116425/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05638763	Recruiting	Chronic Myeloid Leukemia Chronic Phase	Hospital Universitario Dr. Jose E. Gonzalez\|Fernando De la Garza Salazar	November 2024	Phase 2
NCT06385028	Not yet recruiting	Osteoarthritis	Luxembourg Institute of Health\|Œuvre Nationale de Secours Grande Duchesse Charlotte	October 1 2024	Not Applicable

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