Name: DFO
Brand: Selleck Chemicals
SKU: S6849
Availability: InStock
Rating: 5 (18 reviews)

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Quality Control

Batch: Purity: 99.88%

99.88

Related Targets	PROTAC Others Antineoplastic and Immunosuppressive Antibiotics ADC Cytotoxin Selection Antibiotics for Transfected Cell Antibiotics for Mammalian Cell Culture ADC Linker Molecular Glues Hydrotropic Agents Antibiotics for Plant Cell Culture
Other Dyes Inhibitors	Fluorofurimazine (FFz) CFSE (CFDA-SE) Propidium Iodide Thiazolyl Blue Hoechst 33342 D-Luciferin Crystal Violet D-Luciferin sodium salt Dihydroethidium WST-8

Solubility

In vitro
Batch:

DMSO : 36 mg/mL (197.6 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 2 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.8mg/ml (9.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 36 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.25mg/ml (1.37mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	182.18	Formula	C₁₁H₆N₂O	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	54078-29-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	9H-1,8-Diazafluoren-9-one, 1,8-Diazafluoren-9-one			SMILES	C1=CC2=C(C(=O)C3=C2C=CC=N3)N=C1

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	amino acids
References	[1] https://pubmed.ncbi.nlm.nih.gov/26254628/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-06-15)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06447103	RECRUITING	Clear Cell Renal Cell Carcinoma; Sarcomatoid Renal Cell Carcinoma; Stage II Renal Cell Cancer; Stage III Renal Cell Cancer; Stage IV Renal Cell Cancer	Jonsson Comprehensive Cancer Center	2024-08-06	PHASE2
NCT07664397	NOT_YET_RECRUITING	Bladder Cancer; Nerve Sheath Tumor; Nerve Sheath Tumors; Solid Tumor Malignancies; Solid Tumor Cancer; Solid Tumor Neoplasms; Advanced Solid Tumor; Bladder Neoplasm; Nerve Sheath Neoplasms; Nerve Sheath Tumor, Nos; Solid Tumor, Adult; Solid Carcinoma	Robert Flavell, MD, PhD	2026-08-01	PHASE1
NCT03546335	SUSPENDED	Rheumatoid Arthritis	Robert Flavell, MD, PhD	2018-10-01	PHASE1
NCT07179770	RECRUITING	Metastatic Clear Cell Renal Cell Carcinoma	NYU Langone Health	2025-09-15	PHASE1
NCT06439914	RECRUITING	Non Small Cell Lung Cancer	Nerissa T. Viola	2026-03-09	PHASE1
NCT06894745	RECRUITING	Solid Tumor; Solid Carcinoma; Soft Tissue Lesion	Robert Flavell, MD, PhD	2025-06-02	PHASE1

Tech Support

Handling Instructions

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DFO Dyes chemical

Chemical Structure

Molecular Weight: 182.18