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DFO

Dyes chemical

Catalog No.S6849 Purity:99.88%

research use only

DFO (9H-1,8-Diazafluoren-9-one) is a fluorochrome that is used in the forensic field to enhance latent fingerprints deposited on porous surfaces by reacting with the amino acids.

DFO Chemical Structure

Molecular Weight: 182.18

Purity & Quality Control

Batch: Purity: 99.88%

99.88

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Mechanism of Action

Description

DFO (9H-1,8-Diazafluoren-9-one) is a fluorochrome that is used in the forensic field to enhance latent fingerprints deposited on porous surfaces by reacting with the amino acids.

Targets

amino acids ^[1]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06066801	Recruiting	Subjects With Diabetes and DFU With a Suspicion of DFO	Assistance Publique - Hôpitaux de Paris	March 14 2024	Not Applicable
NCT06204237	Recruiting	Peripheral Arterial Occlusive Disease\|Critical Limb Ischemia	Aplagon Oy\|TRACER Europe BV	February 1 2024	Early Phase 1
NCT05974579	Recruiting	Amyotrophic Lateral Sclerosis	Université de Sherbrooke\|Eli Lilly and Company\|Chorus Wellness Inc.\|AL-S Pharma AG	November 23 2023	Phase 1
NCT05955833	Recruiting	Breast Cancer\|Metastatic Breast Cancer\|HER2-positive Breast Cancer\|Gastric Cancer\|Metastatic Gastric Cancer\|HER2-positive Gastric Cancer	Amsterdam UMC location VUmc\|AstraZeneca	June 26 2023	Phase 1

References
https://pubmed.ncbi.nlm.nih.gov/26254628/

References

https://pubmed.ncbi.nlm.nih.gov/26254628/

Chemical Information

Molecular Weight	182.18	Formula	C₁₁H₆N₂O
CAS No.	54078-29-4	SDF	--
Synonyms	9H-1,8-Diazafluoren-9-one, 1,8-Diazafluoren-9-one
Smiles	C1=CC2=C(C(=O)C3=C2C=CC=N3)N=C1

Storage and Stability

Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 36 mg/mL ( (197.6 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 2 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.8mg/ml (9.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 36 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.25mg/ml (1.37mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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