CAS No. 58-85-5

Batch: Purity:99.91
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Biotin (Vitamin B7) Chemical Structure
Chemical Structure
Check the Biotin (Vitamin B7) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid
CAS Number 58-85-5
Molecular Formula

C10H16N2O3S
Molecular Weight (MW) 244.31
SMILES C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2
InChIKey YBJHBAHKTGYVGT-ZKWXMUAHSA-N

Ordering Information

Biological Activity

Biotin (Vitamin B7) is an essential water-soluble B-complex vitamin that acts as an indispensable coenzyme for biotin-dependent carboxylases involved in fatty acid synthesis, amino acid catabolism, and gluconeogenesis. Beyond its metabolic coenzyme roles, biotin is covalently attached to lysine residues in histones, modulating chromatin structure and transcription factors to regulate gene expression and cell proliferation. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 49 mg/mL (200.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.