4-Aminoantipyrine

Catalog No.S4509 Synonyms: Ampyrone, NSC 60242, Metapirazone, Solnapyrin-A, Solvapyrin-A, Minoazophene, Aminoazophene, Aminoantipyrin

For research use only.

4-Aminoantipyrine(Ampyrone) is a metabolite of aminopyrine with analgesic, anti-inflammatory, and antipyretic properties. It is used as a reagent for biochemical reactions producing peroxides or phenols.

4-Aminoantipyrine Chemical Structure

CAS No. 83-07-8

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Biological Activity

Description 4-Aminoantipyrine(Ampyrone) is a metabolite of aminopyrine with analgesic, anti-inflammatory, and antipyretic properties. It is used as a reagent for biochemical reactions producing peroxides or phenols.
In vitro

The level of glutathione(GSH) in single erythrocytes is impacted slightly at low AAP(4-aminoantipyrine) concentrations probably due to the protection of glutathione reductase. When AAP concentration further increases, AAP has a significant influence on GSH[2].

In vivo 4-aminoantipyrine (4-AA) is not genotoxic and mutagenic in vivo, it can interfere with DNA damaging agents biological activities and may reduce the effectiveness of DNA damage-based chemotherapy[3].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Human erythrocytes
  • Concentrations: 1 × 10−6 mol/L to 1 × 10−3 mol/L
  • Incubation Time: 2 h
  • Method:

    Erythrocytes in PBS are incubated for 2 h at ambient temperature (without mixing) with AAP in concentrations from 1 × 10−6 mol/L to 1 × 10−3 mol/L. Sample of erythrocytes without AAP is used as control. After the exposure of AAP, erythrocytes are washed with borate buffer (pH 9.0) 2 times by centrifuging and determined for GSH.

Animal Research:

[1]

  • Animal Models: Male Wistar rats
  • Dosages: 240 µmol/kg
  • Administration: iv(caudal vein)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 203.24
Formula

C11H13N3O

CAS No. 83-07-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(=O)N(N1C)C2=CC=CC=C2)N

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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