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Fisetin Natural Flavonoid

Name: Fisetin
Brand: Selleck Chemicals
SKU: S2298
Availability: InStock
Rating: 5 (43 reviews)

Cat.No.S2298

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

Chemical Structure

Molecular Weight: 286.24

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	Epigenetic Reader Domain HDAC JAK Histone Methyltransferase AMPK PKC PARP HIF Histone Acetyltransferase Aurora Kinase PRMT Histone Demethylase EZH1/2 DNA Methyltransferase Pim LSD1 MLL JMJD G9a/GLP NSD FTO MicroRNA PAD SETD SMYD DOT1 COMT WDR5 HNMT SAM MTase
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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description
MDA-MB-453	Cell viability assay	0, 20, 40, 60, 80, and 100 µM	24, 48, and 72 hours	With increasing concentrations of fisetin (0, 20, 40, 60, 80, and 100 µM), there was a decrease observed in cell number in different time intervals (24, 48, and 72 hours).
SGC7901	Proliferation assay	0, 1, 5, 10, 15 or 20 µM	48 h	inhibits proliferation
A549	Cell viability assay	0, 10, 20, 30 or 40 µM	24, 48 or 72 h	fisetin was effective in inhibiting the growth of A549 cells in a dose- and time-dependent manner
ARPE-19	Cell migration assay	5 or 10 μM	24 h	pretreatment with fisetin markedly reduced cell migration in EGF-treated ARPE-19 cells
SCC-23	Cell viability assay	0-100 μM	24 h	fisetin significantly inhibited cell viability in a dose-dependent manner (significantly when the concentration is higher than 20 μM)
Tca-8113	Cell viability assay	0-100 μM	24 h	fisetin significantly inhibited cell viability in a dose-dependent manner (significantly when the concentration is higher than 20 μM)
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	286.24	Formula	C₁₅H₁₀O₆	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	528-48-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

DMSO : 57 mg/mL ( (199.13 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 9 mg/mL

Water : Insoluble

DMSO : 750 mg/mL ( (2620.17 mM) Warmed with 50°C water bath; Ultrasonicated; Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (34.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Sirtuin ^[1]
In vitro	Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.^[2]
In vivo	Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.^[2]
References	https://pubmed.ncbi.nlm.nih.gov/19075764/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597709/

Applications

Methods	Biomarkers	PMID
Western blot	p-IKKα/β / IKKβ / IKKα / p-IκBα / IκBα / p-p65 / p65 XBP1s / ATF6 / CHOP / ATF4 / PERK DR3 / pNF-κB / NF-κB p-mTOR / mTOR / Raptor / Rictor	23861901
Immunofluorescence	p-4EBP1 / p-eIF4B / p-rpS6 uPA	20530556
Growth inhibition assay	Cell viability	19670328