3-TYP

Catalog No.S8628 Batch:S862801

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Technical Data

Formula

C7H6N4
Molecular Weight 146.15 CAS No. 120241-79-4
Solubility (25°C)* In vitro DMSO 100 mg/mL (684.22 mM)
Ethanol 29 mg/mL (198.42 mM)
Water 1.25 mg/mL (8.55 mM)
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
10%DMSO 90%Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 3.340mg/ml (22.85mM) Taking the 1 mL working solution as an example, add 100 μL of 33.4 mg/ml clear DMSO stock solution to 900 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 3-TYP (3-(1H-1,2,3-triazol-4-yl) pyridine) is a selective SIRT3 inhibitor which is selective for Sirt3 over Sirt1 and Sirt2. The IC50 values for SITR1, SIRT2, SIRT3 are 88 nM, 92 nM, 16 nM respectively.
Targets
SIRT3 [1]
(cell-free)		 SIRT1 [1]
(cell-free)		 SIRT2 [1]
(cell-free)
16 nM 88 nM 92 nM
In vitro Melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity are significantly attenuated by 3-TYP in HepG2 cells exposed to Cd (cadmium).

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    HepG2 cells

  • Concentrations

    50 μM

  • Incubation Time

    12 h

  • Method

    Cells are randomly divided into the following treatment groups: 10 μM Cd; melatonin (1 μM)+10 μM Cd; 3-TYP (50 μM)+10 μM Cd; and this compound+melatonin (1 μM)+10 μM Cd. cells are treated for 12 h. The role of the SIRT3-SOD2 pathway in hepatic cell protection after melatonin pretreatment is investigated.
Animal Study:

[3]
  • Animal Models

    Sprague-Dawley rats

  • Dosages

    5 mg/kg

  • Administration

    i.v.

References

  • https://pubmed.ncbi.nlm.nih.gov/22835719/
  • https://pubmed.ncbi.nlm.nih.gov/26120888/
  • https://pubmed.ncbi.nlm.nih.gov/27067422/

Customer Product Validation

Effects of HNK or 3‐TYP on surgery/anesthesia‐induced an increased expression of pro‐inflammatory cytokines in hippocampus. (A, E) Representative blots at postoperative 1 and 3 d, respectively. (B, F) MCP‐1 relative expression. (C, G) TNF‐α relative expression. (D, H) IL‐1β relative expression. The data are presented as the mean ±standard error of the mean for each group (n = 6 per group). *P < 0.05 vs the control group; #P < 0.05 vs the surgery+vehicle group; $P < 0.05 vs the honokiol group

Data from [ , , CNS Neurosci Ther, 2018, doi:10.1111/cns.13053 ]

Selleck's 3-TYP Has Been Cited by 45 Publications

Caffeine Targets SIRT3 to Enhance SOD2 Activity in Mitochondria [ Frontiers in Cell and Developmental Biology, September 1, 2020, 822] PubMed: 33015038
Intermedin Alleviates Vascular Calcification in CKD through Sirtuin 3-Mediated Inhibition of Mitochondrial Oxidative Stress [ Pharmaceuticals, October 2, 2022, 1224] PubMed: 36297336
Dysregulation of SIRT3 SUMOylation Confers AML Chemoresistance via Controlling HES1-Dependent Fatty Acid Oxidation [ International Journal of Molecular Sciences, July 27, 2022, 8282] PubMed: 35955415
Inhibition of Sirtuin 3 prevents titanium particle-induced bone resorption and osteoclastsogenesis via suppressing ERK and JNK signaling [ International Journal of Biological Sciences, April 3, 2021, 1382-1394] PubMed: 33867853
Paeoniflorin Ameliorates Spinal Cord Injury by Controlling Apoptosis and Ferroptosis in H2O2-Damaged PC12 Cells [ Immun Inflamm Dis, 2026, 14(1):e70324] PubMed: 41560458
HAT1/HDAC2 mediated ACSL4 acetylation confers radiosensitivity by inducing ferroptosis in nasopharyngeal carcinoma [ Cell Death Dis, 2025, 16(1):160] PubMed: 40050614
Apelin/APJ alleviates diabetic nephropathy by improving glomerular endothelial cells dysfunction via SIRT3‑KLF15 [ Mol Med Rep, 2025, 31(5)122] PubMed: 40052569
Starvation-induced phosphorylation activates gasdermin A to initiate pyroptosis [ Cell Rep, 2024, 43(9):114728] PubMed: 39264808
Salmonella-induced SIRT1 and SIRT3 are crucial for maintaining the metabolic switch in bacteria and host for successful pathogenesis [ Elife, 2024, 13RP93125] PubMed: 39693143
KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation [ PLoS Pathog, 2024, 20(3):e1012082] PubMed: 38470932

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.