Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

Size Price Stock Quantity  
USD 127 In stock
USD 397 In stock
USD 997 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

3 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MlqzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1W3SWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55M{C5OUDPxE1? MYPTRW5ITVJ?
human H460a cells MWXQdo9tcW[ncnH0bY9vKGG|c3H5 M1;nd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSES2NIEh[2WubIOsJGlEPTB;MT6wOEDPxE1? M2LtXFE4OzB|NEKx
human MDA435 cells M1TnR3Bzd2yrZnXyZZRqd25iYYPzZZk> NIjS[JBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERVQ{PSClZXzsd{whUUN3ME2xMlE1KM7:TR?= MVuxO|MxOzR{MR?=
human HCT116 cells MXjQdo9tcW[ncnH0bY9vKGG|c3H5 M1fBRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzMEBKSzVyPUGuNVQh|ryP Mn31NVc{ODN2MkG=
human H4 cell MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFfGVo5KdmirYnn0bY9vKG:oIHj1cYFvKEh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6xOVg4PSEQvF2= MXTTRW5ITVJ?
human PA-1 cell NUTR[lk5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYj1S|ZKUW6qaXLpeIlwdiCxZjDoeY1idiCSQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zOjZ3NDFOwG0> MlHJV2FPT0WU
human T98G cell M1fGPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml7OTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zOlgyOiEQvF2= NU\mdHJ[W0GQR1XS
human SW1710 cell NG\HeIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGnkXXZKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjF2MEig{txO MljBV2FPT0WU
human U251 cell NYruclc{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NE\rRm9KdmirYnn0bY9vKG:oIHj1cYFvKFV{NUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY{PzdzIN88US=> NFq1bI5USU6JRWK=
human ALL-PO cell Ml32S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3fwOGlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0yNjlyOEGzJO69VQ>? MVjTRW5ITVJ?
human RKO cells MojYVJJwdGmoZYLheIlwdiCjc4PhfS=> M{nqfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUlvPJINmdGy|LDDJR|UxRTJizszN MXyxO|MxOzR{MR?=
human SK-N-DZ cell M{[3Xmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIrTOlRKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjBzOEe2JO69VQ>? NVHEdVRqW0GQR1XS
human A4-Fuk cell M2jTe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJGE1NU[3azDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNFU3ODRizszN NIrMTpJUSU6JRWK=
human SW480 cells MYHQdo9tcW[ncnH0bY9vKGG|c3H5 NI\QUXhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPXOFgxKGOnbHzzMEBKSzVyPUKuNkDPxE1? NInLdGwyPzNyM{SyNS=>
human 639-V cell NYD5c4F7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKGi3bXHuJFY{QS2YIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6yO|U{PyEQvF2= MnTVV2FPT0WU
human Hs-578-T cell NXHxVo55T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWXPPY1vUW6qaXLpeIlwdiCxZjDoeY1idiCKcz21O|guXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMke3PVkh|ryP NH;2WIJUSU6JRWK=
human UM-UC-3 cell MkfyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJHVONVWFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVIvPzN7M{[g{txO MXzTRW5ITVJ?
human BPH-1 cell Mnm5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXXzNohNUW6qaXLpeIlwdiCxZjDoeY1idiCEUFitNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTd2NUWg{txO Mnv2V2FPT0WU
human 5637 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXjndGpRUW6qaXLpeIlwdiCxZjDoeY1idiB3NkO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4xQDV|MjFOwG0> NUXEXJdwW0GQR1XS
human 8-MG-BA cell M4\T[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUDrNIFCUW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1|LkG0NFQ6KM7:TR?= MVvTRW5ITVJ?
human HCT116 cells MVTDfZRwfG:6aXRCpIF{e2G7 NHzQb3E2KGSjeYO= NGDqOYlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5Nige,:jDDJR|UxRTNwMjFOwG0> MUOyNlMzPjF4OB?=
human LB1047-RCC cell NY\re3JMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoKzTY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{WwNUDPxE1? M4jVXHNCVkeHUh?=
human KOSC-2 cell MmqyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVjJcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOFQ3PjZ{IN88US=> NHzNWGlUSU6JRWK=
human COR-L88 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFW1dmZKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR5NUizJO69VQ>? NHHMSZlUSU6JRWK=
human PSN1 cell MonqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYTyeZJqUW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yODhyNTFOwG0> M3jrN3NCVkeHUh?=
human LXF-289 cell NX3o[HU5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnPSTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOTd4IN88US=> MkXaV2FPT0WU
human MN-60 cell Mlf5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlnUTY5pcWKrdHnvckBw\iCqdX3hckBOVi14MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuN|c3OjNizszN MV\TRW5ITVJ?
human NEC8 cell M3rFN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVPvTmNDUW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE41PzB5NjFOwG0> MlLGV2FPT0WU
human SJSA1 cells Mne1VJJwdGmoZYLheIlwdiCjc4PhfS=> M2G4dmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1rTRVEh[2WubIOsJGlEPTB;ND61OUDPxE1? MmHzNVc{ODN2MkG=
human NCI-H2405 cell M4Lmdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH7FNHFKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlQxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwN{S0PVMh|ryP MkCxV2FPT0WU
human YH-13 cell NXHRe|hnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVTMXXZzUW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzV5N{[g{txO NV3Pe2NRW0GQR1XS
human SK-MEL-2 cell M{nBbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGrpbWZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS5yOU[zNkDPpG1? MkHJV2FPT0WU
human NB13 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoH2TY5pcWKrdHnvckBw\iCqdX3hckBPSjF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zNlc5PiEQvF2= NWr4d5A4W0GQR1XS
human GAMG cell NHn4SINIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEH4TWlKdmirYnn0bY9vKG:oIHj1cYFvKEeDTVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM4OTVizszN MmnQV2FPT0WU
human NCI-H1792 cell NWnXRo1oT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWTJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPDd7NUSg{txO M3\2U3NCVkeHUh?=
human KP-4 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGXDcXJKdmirYnn0bY9vKG:oIHj1cYFvKEuSLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlY6QDJ|IN88US=> M13pZ3NCVkeHUh?=
human HGC-27 cell NYK5dFNPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWjsflA5UW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd|MEe3JO69VQ>? M2jsNHNCVkeHUh?=
human GCT cell M1;obmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NULKRnFzUW6qaXLpeIlwdiCxZjDoeY1idiCJQ2SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlg2OTJzIN88US=> NYTkXG1sW0GQR1XS
human PFSK-1 cell NWq5T4RGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoT6TY5pcWKrdHnvckBw\iCqdX3hckBRTlONLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlg3OTZ6IN88US=> NY\qN3l5W0GQR1XS
human A172 cell MkLOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGEyPzJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkmwO|kh|ryP NXLGNGJ1W0GQR1XS
human HO-1-N-1 cell NXnHT4pDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{PnS2lvcGmkaYTpc44hd2ZiaIXtZY4hUE9vMT3OMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl3NEK1JO69VQ>? MYfTRW5ITVJ?
human HN cell Ml7NS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{fqemlvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lkm3NFk4KM7:TR?= MlvSV2FPT0WU
human IST-MES1 cell NVXUc4VtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmHFTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvTVXTNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTd3MkGg{txO MXLTRW5ITVJ?
human TGW cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYDnb2REUW6qaXLpeIlwdiCxZjDoeY1idiCWR2egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2Pjh{IN88US=> M3G2XXNCVkeHUh?=
human COR-L23 cell NXfQcYVPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFOzVZpKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MNlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjN5M{Wg{txO M2nSXnNCVkeHUh?=
human D-566MG cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlKxTY5pcWKrdHnvckBw\iCqdX3hckBFNTV4Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok44QDh5MzFOwG0> NX\SVnRjW0GQR1XS
human SKG-IIIa cell Ml3US5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWj2U|QyUW6qaXLpeIlwdiCxZjDoeY1idiCVS1etTWlK[SClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwOEW1O|Ih|ryP MVjTRW5ITVJ?
human NCI-H446 cell M1PMc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkfvTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSES0OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODF6MTFOwG0> NVvaZWVMW0GQR1XS
human KYSE-270 cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUDJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOzh5MjFOwG0> MlzLV2FPT0WU
human CAL-39 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{nOcmlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4{PTJ2IN88US=> MmnLV2FPT0WU
human IST-SL1 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWXJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ{OzR7IN88US=> NEjwV3VUSU6JRWK=
human SF539 cell NGH1[pRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3\BRmlvcGmkaYTpc44hd2ZiaIXtZY4hW0Z3M{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlU2OTl5IN88US=> NWfMbHZwW0GQR1XS
human TE-1 cell M1XpcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2\JVGlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPFkxQTFizszN M4T4RnNCVkeHUh?=
human NB10 cell MkDCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWjDfI1[UW6qaXLpeIlwdiCxZjDoeY1idiCQQkGwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QDd3ODFOwG0> NEnpcpNUSU6JRWK=
human 8505C cell NVzUZnE2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFi0PGhKdmirYnn0bY9vKG:oIHj1cYFvKDh3MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QThzNzFOwG0> MlXFV2FPT0WU
human DB cell NV7rfmY2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUHJcohq[mm2aX;uJI9nKGi3bXHuJGRDKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5yMUGzNUDPxE1? MlTOV2FPT0WU
human NCI-H28 cell NUL3PIpST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2PXXWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPTl6OESg{txO M3P3bnNCVkeHUh?=
human LOXIMVI cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGxQYEmPVlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY{PzBzIN88US=> NHz5VJZUSU6JRWK=
human ME-180 cell NU\TdZp6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1\1XWlvcGmkaYTpc44hd2ZiaIXtZY4hVUVvMUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE45ODN7NTFOwG0> NXPRVlZGW0GQR1XS
human ES3 cell MmfmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTlwMUK3PVQh|ryP NX3hWlNTW0GQR1XS
human SK-N-FI cell NU\3eHA{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlXSTY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLV\JJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU41OTZ7OTFOwG0> MlfpV2FPT0WU
human NBsusSR cell M4LJUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWO2Z3RNUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwMkOxPEDPxE1? M4rOcnNCVkeHUh?=
human MCF7 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYnQ[I1PUW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTh2OTFOwG0> MUTTRW5ITVJ?
human A549 cell MnjTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYHiSJNIUW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB4IN88US=> MWDTRW5ITVJ?
human HuP-T4 cell MofUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVPJcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlQzPzFizszN M2L3[3NCVkeHUh?=
human HSC-2 cell NH;jcYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXhTY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMFc5PDJizszN MXXTRW5ITVJ?
human A253 cell M2PGVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;tNnRKdmirYnn0bY9vKG:oIHj1cYFvKEF{NUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4zQDN2IN88US=> M3zlVXNCVkeHUh?=
human HCT-116 cell M3zkZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYP3W5dnUW6qaXLpeIlwdiCxZjDoeY1idiCSQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVgvODB2MzFOwG0> M{OzUXNCVkeHUh?=
human HCT-116 cell M3OydWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmXCTY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVkvPjZ6IN88US=> MnTMV2FPT0WU

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
+ Expand

CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
+ Expand
  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

CDK Signaling Pathway Map

Related CDK Products

Tags: buy Ro-3306 | Ro-3306 supplier | purchase Ro-3306 | Ro-3306 cost | Ro-3306 manufacturer | order Ro-3306 | Ro-3306 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID