Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NXHRe2lyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NY\PTW5FUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtV25WNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkezNFk2KM7:TR?= M{DEfnNCVkeHUh?=
human H460a cells Mn7SVJJwdGmoZYLheIlwdiCjc4PhfS=> M2\6O2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSES2NIEh[2WubIOsJGlEPTB;MT6wOEDPxE1? M13ZSlE4OzB|NEKx
human MDA435 cells Mn;hVJJwdGmoZYLheIlwdiCjc4PhfS=> NVztPWhUSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGE1OzViY3XscJMtKEmFNUC9NU4yPCEQvF2= NFH3bJMyPzNyM{SyNS=>
human HCT116 cells NIm3bZdRem:uaX\ldoF1cW:wIHHzd4F6 NYTIV41XSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMtKEmFNUC9NU4yPCEQvF2= MWCxO|MxOzR{MR?=
human H4 cell NUXMNmZuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGTXUVBKdmirYnn0bY9vKG:oIHj1cYFvKEh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6xOVg4PSEQvF2= MXPTRW5ITVJ?
human PA-1 cell NIrKUYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXHiWIpXUW6qaXLpeIlwdiCxZjDoeY1idiCSQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zOjZ3NDFOwG0> NHXTVpNUSU6JRWK=
human T98G cell M4XLc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NED0Z4NKdmirYnn0bY9vKG:oIHj1cYFvKFR7OFegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlM3QDF{IN88US=> M4Dpd3NCVkeHUh?=
human SW1710 cell M1:xbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFWzcW9KdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjF2MEig{txO M2e4O3NCVkeHUh?=
human U251 cell Mo\NS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWPFbmdsUW6qaXLpeIlwdiCxZjDoeY1idiCXMkWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43Ozd5MTFOwG0> MWTTRW5ITVJ?
human ALL-PO cell M3j3WWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEfqZYFKdmirYnn0bY9vKG:oIHj1cYFvKEGOTD3QU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:OS57MEixN{DPxE1? MYrTRW5ITVJ?
human RKO cells NWXjR|FJWHKxbHnm[ZJifGmxbjDhd5NigQ>? NWjETIduSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOsJGlEPTB;MjFOwG0> NUX2O5REOTd|MEO0NlE>
human SK-N-DZ cell MljkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmHjTY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLVTaJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4xOTh5NjFOwG0> M4HrVXNCVkeHUh?=
human A4-Fuk cell NUPJV3hKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWD2Zo1ZUW6qaXLpeIlwdiCxZjDoeY1idiCDND3GeYsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB3NkC0JO69VQ>? MVzTRW5ITVJ?
human SW480 cells NIHGTmRRem:uaX\ldoF1cW:wIHHzd4F6 NEDLfXZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPXOFgxKGOnbHzzMEBKSzVyPUKuNkDPxE1? M4Dw[lE4OzB|NEKx
human 639-V cell MmnqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJFY{QS2YIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6yO|U{PyEQvF2= MUDTRW5ITVJ?
human Hs-578-T cell MorFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXfET3FDUW6qaXLpeIlwdiCxZjDoeY1idiCKcz21O|guXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMke3PVkh|ryP MXTTRW5ITVJ?
human UM-UC-3 cell NEHaOXhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGf1[GRKdmirYnn0bY9vKG:oIHj1cYFvKFWPLWXDMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTJwN{O5N|Yh|ryP MlLHV2FPT0WU
human BPH-1 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVHJcohq[mm2aX;uJI9nKGi3bXHuJGJRUC1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj65O|Q2PSEQvF2= NGK2SZhUSU6JRWK=
human 5637 cell MljJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmG2TY5pcWKrdHnvckBw\iCqdX3hckA2PjN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6wPFU{OiEQvF2= NYPhV4NsW0GQR1XS
human 8-MG-BA cell NUXaVJh5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVnTWZlzUW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1|LkG0NFQ6KM7:TR?= NXj5fWxGW0GQR1XS
human HCT116 cells NILhfmhEgXSxdH;4bYPDqGG|c3H5 M{TY[VUh\GG7cx?= Mo\yR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGGodHXyJFUh\GG7czDifUBOXFNiYYPzZZnwxIxiSVO1NF0{NjJizszN MV6yNlMzPjF4OB?=
human LB1047-RCC cell NFTaPXZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXPMNI9NUW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yN|UxOSEQvF2= NVHwPWRYW0GQR1XS
human KOSC-2 cell MnfYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXvJcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOFQ3PjZ{IN88US=> NYjaSIpwW0GQR1XS
human COR-L88 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M33CWWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVy4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDd3OEOg{txO M3Xue3NCVkeHUh?=
human PSN1 cell MnnkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWjhWoNYUW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yODhyNTFOwG0> NHLK[|ZUSU6JRWK=
human LXF-289 cell MlHaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MojhTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOTd4IN88US=> MXjTRW5ITVJ?
human MN-60 cell M4PsSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2fwOmlvcGmkaYTpc44hd2ZiaIXtZY4hVU5vNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlM4PjJ|IN88US=> MmnHV2FPT0WU
human NEC8 cell NV;CdHdLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4q4OWlvcGmkaYTpc44hd2ZiaIXtZY4hVkWFODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFcxPzZizszN MUXTRW5ITVJ?
human SJSA1 cells NFPBelJRem:uaX\ldoF1cW:wIHHzd4F6 MkTVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSmPBNUBk\WyuczygTWM2OD12LkW1JO69VQ>? M{fic|E4OzB|NEKx
human NCI-H2405 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M323bGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyOFA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC55NES5N{DPxE1? M{HnOXNCVkeHUh?=
human YH-13 cell NWPwcWZ{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWDJcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND63OVc4PiEQvF2= MXfTRW5ITVJ?
human SK-MEL-2 cell NXXlOY9RT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn7ZTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvODl4M{Kg{rxu NXXhcJFwW0GQR1XS
human NB13 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3TKOWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|I4QDZizszN NF;mNHpUSU6JRWK=
human GAMG cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mki3TY5pcWKrdHnvckBw\iCqdX3hckBISU2JIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zO|E2KM7:TR?= MX7TRW5ITVJ?
human NCI-H1792 cell MofMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlzDTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG3PVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjR5OUW0JO69VQ>? MVjTRW5ITVJ?
human KP-4 cell MoC3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkC1TY5pcWKrdHnvckBw\iCqdX3hckBMWC12IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT62PVgzOyEQvF2= NX70S2F3W0GQR1XS
human HGC-27 cell MnSxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NG\zTZpKdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzNyN{eg{txO NEC5PJBUSU6JRWK=
human GCT cell MkHoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3HDfGlvcGmkaYTpc44hd2ZiaIXtZY4hT0OWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT64OVEzOSEQvF2= NHy4UpZUSU6JRWK=
human PFSK-1 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN M2XJT3NCVkeHUh?=
human A172 cell M170[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGLnZ3FKdmirYnn0bY9vKG:oIHj1cYFvKEFzN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlkxPzlizszN M4PCXnNCVkeHUh?=
human HO-1-N-1 cell NYW4RWFyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKGi3bXHuJGhQNTFvTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PTR{NTFOwG0> M1PyNXNCVkeHUh?=
human HN cell NGHoUYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzITY5pcWKrdHnvckBw\iCqdX3hckBJViClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUewPVch|ryP NX3mXGF4W0GQR1XS
human IST-MES1 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIGyTmZKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3NSXMyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{WyNUDPxE1? M2jrTXNCVkeHUh?=
human TGW cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkDlTY5pcWKrdHnvckBw\iCqdX3hckBVT1diY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkC1OlgzKM7:TR?= MUHTRW5ITVJ?
human COR-L23 cell NIDhSHFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlPTTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEKzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{PzN3IN88US=> M13R[3NCVkeHUh?=
human D-566MG cell NFHSSHNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIHvbHFKdmirYnn0bY9vKG:oIHj1cYFvKERvNU[2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njd6OEezJO69VQ>? NX;rdmZ1W0GQR1XS
human SKG-IIIa cell NHfCRo1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXvJcohq[mm2aX;uJI9nKGi3bXHuJHNMTy2LSVnhJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok45PTV5MjFOwG0> MnnhV2FPT0WU
human NCI-H446 cell NHr3fnZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXXJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNES2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOThzIN88US=> NEf4XldUSU6JRWK=
human KYSE-270 cell NY\EblRjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEDm[GlKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtNlcxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yM{i3NkDPxE1? MoTTV2FPT0WU
human CAL-39 cell Mlz6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGC3RmVKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2zPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOzV{NDFOwG0> NX7JRmVLW0GQR1XS
human IST-SL1 cell NFXueoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFLhXmpKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjR|M{S5JO69VQ>? NFT4ZnpUSU6JRWK=
human SF539 cell M13Tcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFjxNpZKdmirYnn0bY9vKG:oIHj1cYFvKFOINUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{42PTF7NzFOwG0> MV7TRW5ITVJ?
human TE-1 cell MnzPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXLJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15Lki5NFkyKM7:TR?= Mly3V2FPT0WU
human NB10 cell NH[2doJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlnjTY5pcWKrdHnvckBw\iCqdX3hckBPSjFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz65PFc2QCEQvF2= Mki4V2FPT0WU
human 8505C cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVPJcohq[mm2aX;uJI9nKGi3bXHuJFg2ODWFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz65PVgyPyEQvF2= MmjBV2FPT0WU
human DB cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGRDKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5yMUGzNUDPxE1? NIjqb4lUSU6JRWK=
human NCI-H28 cell M1S3ZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVnJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlU6QDh2IN88US=> Mn33V2FPT0WU
human LOXIMVI cell NEPvTnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWT5T3ZJUW6qaXLpeIlwdiCxZjDoeY1idiCOT2jJUXZKKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC54M{ewNUDPxE1? NHzVTldUSU6JRWK=
human ME-180 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHXHRXBKdmirYnn0bY9vKG:oIHj1cYFvKE2HLUG4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvQDB|OUWg{txO M3LZS3NCVkeHUh?=
human ES3 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXLJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTlwMUK3PVQh|ryP M4m4[nNCVkeHUh?=
human SK-N-FI cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEjpN|dKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjRzNkm5JO69VQ>? NHH4eVFUSU6JRWK=
human NBsusSR cell NVXCUG04T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NILYcnRKdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5{M{G4JO69VQ>? MlzNV2FPT0WU
human MCF7 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVPJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6xPFQ6KM7:TR?= NYe4NFhwW0GQR1XS
human A549 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV:yU|RRUW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB4IN88US=> MVHTRW5ITVJ?
human HuP-T4 cell NX;tNlhnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmrjTY5pcWKrdHnvckBw\iCqdX3hckBJfVBvVESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE41OjdzIN88US=> Ml;VV2FPT0WU
human HSC-2 cell NGPoWWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGsO|g1OiEQvF2= MmTPV2FPT0WU
human A253 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUK5SIhLUW6qaXLpeIlwdiCxZjDoeY1idiCDMkWzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh|NDFOwG0> NIm0RnRUSU6JRWK=
human HCT-116 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mkn1TY5pcWKrdHnvckBw\iCqdX3hckBRSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUiuNFA1OyEQvF2= NHPN[3RUSU6JRWK=
human HCT-116 cell NXnIVpViT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MojlTY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVkvPjZ6IN88US=> M3LQO3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID