Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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3 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NXjrW5VMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF3DNYFKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwN{OwPVUh|ryP MoP5V2FPT0WU
human H460a cells MWXQdo9tcW[ncnH0bY9vKGG|c3H5 NYK3cZFmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYx[SClZXzsd{whUUN3ME2xMlA1KM7:TR?= NFzvOZQyPzNyM{SyNS=>
human MDA435 cells Mlq0VJJwdGmoZYLheIlwdiCjc4PhfS=> MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQUSzOUBk\WyuczygTWM2OD1zLkG0JO69VQ>? MX2xO|MxOzR{MR?=
human HCT116 cells NY\JRopZWHKxbHnm[ZJifGmxbjDhd5NigQ>? NHTCNlZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3ME2xMlE1KM7:TR?= NIDhWpEyPzNyM{SyNS=>
human H4 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MofNTY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUW4O|Uh|ryP MYHTRW5ITVJ?
human PA-1 cell Ml7YS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3XjPWlvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlI3PTRizszN NUDmPG9OW0GQR1XS
human T98G cell NITPWVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3vEXWlvcGmkaYTpc44hd2ZiaIXtZY4hXDl6RzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|Y5OTJizszN Mmi5V2FPT0WU
human SW1710 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mor2TY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlYyPDB6IN88US=> NIfSXZRUSU6JRWK=
human U251 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnriTY5pcWKrdHnvckBw\iCqdX3hckBWOjVzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|c4OSEQvF2= MXjTRW5ITVJ?
human ALL-PO cell NYTrSVIzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWLYTWdbUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTFwOUC4NVMh|ryP MUDTRW5ITVJ?
human RKO cells NVj5XVY1WHKxbHnm[ZJifGmxbjDhd5NigQ>? MlfaRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCUS1:gZ4VtdHNuIFnDOVA:OiEQvF2= M2n5RVE4OzB|NEKx
human SK-N-DZ cell NF;H[|BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{XSRWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODF6N{[g{txO MYfTRW5ITVJ?
human A4-Fuk cell M17pT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1TZb2lvcGmkaYTpc44hd2ZiaIXtZY4hSTRvRoXrJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4xPTZyNDFOwG0> NGfvT|RUSU6JRWK=
human SW480 cells NYPrcJg3WHKxbHnm[ZJifGmxbjDhd5NigQ>? MoDsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczygTWM2OD1{LkKg{txO MmfnNVc{ODN2MkG=
human 639-V cell NGnWPHRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJFY{QS2YIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6yO|U{PyEQvF2= NHnId3RUSU6JRWK=
human Hs-578-T cell Ml7JS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2nlNWlvcGmkaYTpc44hd2ZiaIXtZY4hUHNvNUe4MXQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjJ5N{m5JO69VQ>? M4nqOHNCVkeHUh?=
human UM-UC-3 cell NGHRUpVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGe5WXhKdmirYnn0bY9vKG:oIHj1cYFvKFWPLWXDMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTJwN{O5N|Yh|ryP MkjWV2FPT0WU
human BPH-1 cell Mn\MS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGf5N21KdmirYnn0bY9vKG:oIHj1cYFvKEKSSD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk46PzR3NTFOwG0> NXrGfpM1W0GQR1XS
human 5637 cell M2nIOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVnJcohq[mm2aX;uJI9nKGi3bXHuJFU3OzdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkC4OVMzKM7:TR?= NWrZfXc5W0GQR1XS
human 8-MG-BA cell NYfsbGNIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFTXS2pKdmirYnn0bY9vKG:oIHj1cYFvKDhvTVetRmEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTNwMUSwOFkh|ryP NVnNbYpqW0GQR1XS
human HCT116 cells NFzYNJZEgXSxdH;4bYPDqGG|c3H5 MYG1JIRigXN? Ml;lR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGGodHXyJFUh\GG7czDifUBOXFNiYYPzZZnwxIxiSVO1NF0{NjJizszN MkPVNlI{OjZzNki=
human LB1047-RCC cell NH71[5JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzlTY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{WwNUDPxE1? M{TnTHNCVkeHUh?=
human KOSC-2 cell M{G1cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXHJcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOFQ3PjZ{IN88US=> MmfsV2FPT0WU
human COR-L88 cell NH3iOYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHvCWm9KdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR5NUizJO69VQ>? MWfTRW5ITVJ?
human PSN1 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3XuOWlvcGmkaYTpc44hd2ZiaIXtZY4hWFOQMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNVA5ODVizszN MVfTRW5ITVJ?
human LXF-289 cell MorUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX;pWoJPUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5{MUe2JO69VQ>? Mlf2V2FPT0WU
human MN-60 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= NHPVR|ZUSU6JRWK=
human NEC8 cell NXH5XpN[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M13wVGlvcGmkaYTpc44hd2ZiaIXtZY4hVkWFODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFcxPzZizszN NILhZoFUSU6JRWK=
human SJSA1 cells NHr5Xm5Rem:uaX\ldoF1cW:wIHHzd4F6 MmG1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSmPBNUBk\WyuczygTWM2OD12LkW1JO69VQ>? MmHKNVc{ODN2MkG=
human NCI-H2405 cell NF;kcnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4PwdmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyOFA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC55NES5N{DPxE1? MY\TRW5ITVJ?
human YH-13 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUjEUo1UUW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzV5N{[g{txO NXTLfFczW0GQR1XS
human SK-MEL-2 cell M{D1dGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1niZmlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjB7NkOyJO6ddQ>? M1vodXNCVkeHUh?=
human NB13 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4HoNmlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|I4QDZizszN MX7TRW5ITVJ?
human GAMG cell MkTYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWPVbHF[UW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{PzF3IN88US=> MWnTRW5ITVJ?
human NCI-H1792 cell NVT2SZRwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPDd7NUSg{txO M4nBTXNCVkeHUh?=
human KP-4 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXfJcohq[mm2aX;uJI9nKGi3bXHuJGtRNTRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lk[5PFI{KM7:TR?= MnrSV2FPT0WU
human HGC-27 cell NEPtco9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2PYfWlvcGmkaYTpc44hd2ZiaIXtZY4hUEeFLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44OzB5NzFOwG0> NHPK[3RUSU6JRWK=
human GCT cell NXXJdYpOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEDBSohKdmirYnn0bY9vKG:oIHj1cYFvKEeFVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFUyOjFizszN MorrV2FPT0WU
human PFSK-1 cell MorLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN NIG5NGxUSU6JRWK=
human A172 cell MmjSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mlv2TY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT65NFc6KM7:TR?= M2HqN3NCVkeHUh?=
human HO-1-N-1 cell Mn7IS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVTJcohq[mm2aX;uJI9nKGi3bXHuJGhQNTFvTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PTR{NTFOwG0> MY\TRW5ITVJ?
human HN cell MmXzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXzJcohq[mm2aX;uJI9nKGi3bXHuJGhPKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{C5O{DPxE1? MnnDV2FPT0WU
human IST-MES1 cell MnruS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{fTUGlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLV3FV|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl5NUKxJO69VQ>? NWf4PZhCW0GQR1XS
human TGW cell M4\ofWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYXhVJh7UW6qaXLpeIlwdiCxZjDoeY1idiCWR2egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2Pjh{IN88US=> MWLTRW5ITVJ?
human COR-L23 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlPOTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEKzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{PzN3IN88US=> MoLOV2FPT0WU
human D-566MG cell NIW5eWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVX2WXhZUW6qaXLpeIlwdiCxZjDoeY1idiCGLUW2Om1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi55OEi3N{DPxE1? M2GyXnNCVkeHUh?=
human SKG-IIIa cell Mm\uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{jYeGlvcGmkaYTpc44hd2ZiaIXtZY4hW0uJLVnJTYEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njh3NUeyJO69VQ>? NW\nRVd{W0GQR1XS
human NCI-H446 cell MoHOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWm5W29IUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFQ1PiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEG4NUDPxE1? MnLuV2FPT0WU
human KYSE-270 cell NUjuVHNLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3zLXWlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT2yO|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjB|OEeyJO69VQ>? NHLmXIVUSU6JRWK=
human CAL-39 cell NWjMenFJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkfPTY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlM2OjRizszN MnHGV2FPT0WU
human IST-SL1 cell NEXrPHJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4TOOGlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLWPMNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPDN|NEmg{txO MXPTRW5ITVJ?
human SF539 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEe3epVKdmirYnn0bY9vKG:oIHj1cYFvKFOINUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{42PTF7NzFOwG0> M4LhN3NCVkeHUh?=
human TE-1 cell M4LWOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVX4eYFTUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{45QTB7MTFOwG0> NVvOWo8{W0GQR1XS
human NB10 cell MkLsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NInQ[HlKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlk5PzV6IN88US=> NIrEUoFUSU6JRWK=
human 8505C cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHWzcWlKdmirYnn0bY9vKG:oIHj1cYFvKDh3MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QThzNzFOwG0> NHz0Z5lUSU6JRWK=
human DB cell NGPKUmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIS4b2xKdmirYnn0bY9vKG:oIHj1cYFvKESEIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6wNVE{OSEQvF2= NYS0XWNDW0GQR1XS
human NCI-H28 cell NVnDeVhrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{PiWWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPTl6OESg{txO NXLYb4d1W0GQR1XS
human LOXIMVI cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGDzfXpKdmirYnn0bY9vKG:oIHj1cYFvKEyRWFnNWmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjZ|N{CxJO69VQ>? MUTTRW5ITVJ?
human ME-180 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1OyWmlvcGmkaYTpc44hd2ZiaIXtZY4hVUVvMUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE45ODN7NTFOwG0> M2DwU3NCVkeHUh?=
human ES3 cell M1i3c2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4D3bGlvcGmkaYTpc44hd2ZiaIXtZY4hTVN|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT6xNlc6PCEQvF2= NUnKVoFvW0GQR1XS
human SK-N-FI cell NF\VVlJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU5vRlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlQyPjl7IN88US=> MWDTRW5ITVJ?
human NBsusSR cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW\HNoZOUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwMkOxPEDPxE1? MVjTRW5ITVJ?
human MCF7 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHrHbJdKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> NH;ZSG1USU6JRWK=
human A549 cell NUS2fFA5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFzxbG9KdmirYnn0bY9vKG:oIHj1cYFvKEF3NEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4zODZizszN Ml3WV2FPT0WU
human HuP-T4 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFzRZ2JKdmirYnn0bY9vKG:oIHj1cYFvKEi3UD3UOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjR{N{Gg{txO MVLTRW5ITVJ?
human HSC-2 cell NUfROWViT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWTJcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGsO|g1OiEQvF2= NXmyWllNW0GQR1XS
human A253 cell NGnMTGhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX;qS49bUW6qaXLpeIlwdiCxZjDoeY1idiCDMkWzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh|NDFOwG0> MYTTRW5ITVJ?
human HCT-116 cell NFTiSGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkfwTY5pcWKrdHnvckBw\iCqdX3hckBRSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUiuNFA1OyEQvF2= M2jabHNCVkeHUh?=
human HCT-116 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH3W[3RKdmirYnn0bY9vKG:oIHj1cYFvKEiFVD2xNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQS54Nkig{txO NHT3bphUSU6JRWK=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID