Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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3 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell M2r5OWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1v4c2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55M{C5OUDPxE1? MkfYV2FPT0WU
human H460a cells M2fUb3Bzd2yrZnXyZZRqd25iYYPzZZk> NXLN[otqSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYx[SClZXzsd{whUUN3ME2xMlA1KM7:TR?= MoDSNVc{ODN2MkG=
human MDA435 cells MXHQdo9tcW[ncnH0bY9vKGG|c3H5 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQUSzOUBk\WyuczygTWM2OD1zLkG0JO69VQ>? MXuxO|MxOzR{MR?=
human HCT116 cells NIrWfFdRem:uaX\ldoF1cW:wIHHzd4F6 MnrzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOsJGlEPTB;MT6xOEDPxE1? MljuNVc{ODN2MkG=
human H4 cell NY\E[WJPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5zNUi3OUDPxE1? MmDJV2FPT0WU
human PA-1 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWnJcohq[mm2aX;uJI9nKGi3bXHuJHBCNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKyOlU1KM7:TR?= MYHTRW5ITVJ?
human T98G cell NHLrRYFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEXFSVRKdmirYnn0bY9vKG:oIHj1cYFvKFR7OFegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlM3QDF{IN88US=> NX;4NotDW0GQR1XS
human SW1710 cell NWDlToFIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGfyO2tKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjF2MEig{txO NFnLT3JUSU6JRWK=
human U251 cell M3HNbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2fqfGlvcGmkaYTpc44hd2ZiaIXtZY4hXTJ3MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlM4PzFizszN NGqy[|lUSU6JRWK=
human ALL-PO cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1LUPWlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0yNjlyOEGzJO69VQ>? NUOxPHBoW0GQR1XS
human RKO cells MU\Qdo9tcW[ncnH0bY9vKGG|c3H5 NGP2T2RCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLLU{Bk\WyuczygTWM2OD1{IN88US=> NYe1WIxbOTd|MEO0NlE>
human SK-N-DZ cell NV\FXIZnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M17H[2lvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODF6N{[g{txO MnvJV2FPT0WU
human A4-Fuk cell NGHnbolIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUfJcohq[mm2aX;uJI9nKGi3bXHuJGE1NU[3azDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNFU3ODRizszN NFroelFUSU6JRWK=
human SW480 cells MUfQdo9tcW[ncnH0bY9vKGG|c3H5 MlixRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczygTWM2OD1{LkKg{txO MXuxO|MxOzR{MR?=
human 639-V cell NHO2PHBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVjtN2xEUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOjd3M{eg{txO MXjTRW5ITVJ?
human Hs-578-T cell NFfOcm5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1vqfWlvcGmkaYTpc44hd2ZiaIXtZY4hUHNvNUe4MXQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjJ5N{m5JO69VQ>? M1LYdHNCVkeHUh?=
human UM-UC-3 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUXNdJpPUW6qaXLpeIlwdiCxZjDoeY1idiCXTT3VR{0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1{LkezPVM3KM7:TR?= NYm1OG5VW0GQR1XS
human BPH-1 cell NX3WVJB6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVnJcohq[mm2aX;uJI9nKGi3bXHuJGJRUC1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj65O|Q2PSEQvF2= M3TOUnNCVkeHUh?=
human 5637 cell M1vNXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn;QTY5pcWKrdHnvckBw\iCqdX3hckA2PjN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6wPFU{OiEQvF2= M1XzeHNCVkeHUh?=
human 8-MG-BA cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkH2TY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0{NjF2MES5JO69VQ>? MUTTRW5ITVJ?
human HCT116 cells MUPDfZRwfG:6aXRCpIF{e2G7 NHTPV2I2KGSjeYO= MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOUBl[Xm|IHL5JG1VWyCjc4Phfg+9lCCLQ{WwQVMvOiEQvF2= MUSyNlMzPjF4OB?=
human LB1047-RCC cell NWrkVopKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnvyTY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{WwNUDPxE1? MYHTRW5ITVJ?
human KOSC-2 cell MkHOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUHJcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOFQ3PjZ{IN88US=> MXfTRW5ITVJ?
human COR-L88 cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFv3VoRKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR5NUizJO69VQ>? NYC3fXlMW0GQR1XS
human PSN1 cell NF\1cmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYDTe4tpUW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yODhyNTFOwG0> NGHwZnBUSU6JRWK=
human LXF-289 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUjMPJA2UW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5{MUe2JO69VQ>? M1LlUHNCVkeHUh?=
human MN-60 cell NV\oeHN{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWfJcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= NVnpeo51W0GQR1XS
human NEC8 cell MoXxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12LkS3NFc3KM7:TR?= NYnNUZd{W0GQR1XS
human SJSA1 cells MX7Qdo9tcW[ncnH0bY9vKGG|c3H5 Mlm4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSmPBNUBk\WyuczygTWM2OD12LkW1JO69VQ>? NIjXc24yPzNyM{SyNS=>
human NCI-H2405 cell M4W5cGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYXRRZBNUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI1ODViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lke0OFk{KM7:TR?= MXzTRW5ITVJ?
human YH-13 cell MnnGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4D4e2lvcGmkaYTpc44hd2ZiaIXtZY4hYUhvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20Mlc2Pzd4IN88US=> NEHJcItUSU6JRWK=
human SK-MEL-2 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MorzTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvODl4M{Kg{rxu NICxTFFUSU6JRWK=
human NB13 cell M3S5O2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MW\Jcohq[mm2aX;uJI9nKGi3bXHuJG5DOTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkOyO|g3KM7:TR?= NUD6UplVW0GQR1XS
human GAMG cell Mmj4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MonVTY5pcWKrdHnvckBw\iCqdX3hckBISU2JIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zO|E2KM7:TR?= NHT3N3JUSU6JRWK=
human NCI-H1792 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlzTTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG3PVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjR5OUW0JO69VQ>? NInB[5FUSU6JRWK=
human KP-4 cell MojES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE[xSpJKdmirYnn0bY9vKG:oIHj1cYFvKEuSLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlY6QDJ|IN88US=> NHqySGRUSU6JRWK=
human HGC-27 cell NYraPGFVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn;hTY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlc{ODd5IN88US=> M1vkZXNCVkeHUh?=
human GCT cell M{\FVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUDJcohq[mm2aX;uJI9nKGi3bXHuJGdEXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOEWxNlEh|ryP MVTTRW5ITVJ?
human PFSK-1 cell NGXFbWlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPxdpoxUW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njh4MU[4JO69VQ>? NGHZV5BUSU6JRWK=
human A172 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmXmTY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT65NFc6KM7:TR?= MnXIV2FPT0WU
human HO-1-N-1 cell M3vDW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mk\5TY5pcWKrdHnvckBw\iCqdX3hckBJVy1zLV6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTV2MkWg{txO NHzPe5FUSU6JRWK=
human HN cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWj6NnRwUW6qaXLpeIlwdiCxZjDoeY1idiCKTjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVcxQTdizszN NH[zU4xUSU6JRWK=
human IST-MES1 cell Mo\HS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWLJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2PRWOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PzV{MTFOwG0> MmHtV2FPT0WU
human TGW cell NWK2Z2JLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{\5cmlvcGmkaYTpc44hd2ZiaIXtZY4hXEeZIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6wOVY5OiEQvF2= MXzTRW5ITVJ?
human COR-L23 cell M4\YU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVvxNnJ5UW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFI{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi5|N{O1JO69VQ>? MmHLV2FPT0WU
human D-566MG cell NIfWeWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NInreplKdmirYnn0bY9vKG:oIHj1cYFvKERvNU[2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njd6OEezJO69VQ>? M2DJVnNCVkeHUh?=
human SKG-IIIa cell MoH3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmXwTY5pcWKrdHnvckBw\iCqdX3hckBUU0dvSVnJZUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvQDV3N{Kg{txO MnGwV2FPT0WU
human NCI-H446 cell NEnNcFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3zX[GlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OFYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjBzOEGg{txO M4fZNHNCVkeHUh?=
human KYSE-270 cell MkPQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYrJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOzh5MjFOwG0> MkfhV2FPT0WU
human CAL-39 cell NUHobndZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWm1U45RUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytN|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjN3MkSg{txO M13VXnNCVkeHUh?=
human IST-SL1 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFz4T4hKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjR|M{S5JO69VQ>? MWTTRW5ITVJ?
human SF539 cell NV:ze3BXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz61OVE6PyEQvF2= MULTRW5ITVJ?
human TE-1 cell Ml;HS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWn5cGhUUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{45QTB7MTFOwG0> M1nEOXNCVkeHUh?=
human NB10 cell M4PvcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3TPb2lvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVg4PThizszN NWW3[FFGW0GQR1XS
human 8505C cell M3f5fmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXfJcohq[mm2aX;uJI9nKGi3bXHuJFg2ODWFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz65PVgyPyEQvF2= MlLBV2FPT0WU
human DB cell NHfWZ2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NG\5[FRKdmirYnn0bY9vKG:oIHj1cYFvKESEIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6wNVE{OSEQvF2= NGfsVo5USU6JRWK=
human NCI-H28 cell NGrhZpJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlU6QDh2IN88US=> MWHTRW5ITVJ?
human LOXIMVI cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXPJcohq[mm2aX;uJI9nKGi3bXHuJGxQYEmPVlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY{PzBzIN88US=> M{XISXNCVkeHUh?=
human ME-180 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFXZVYJKdmirYnn0bY9vKG:oIHj1cYFvKE2HLUG4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvQDB|OUWg{txO MYPTRW5ITVJ?
human ES3 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUnJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTlwMUK3PVQh|ryP NWHTRnRQW0GQR1XS
human SK-N-FI cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH7wcIdKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjRzNkm5JO69VQ>? M3HXdXNCVkeHUh?=
human NBsusSR cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnrXTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOjNzODFOwG0> MoPBV2FPT0WU
human MCF7 cell NX7ndFA1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH[2[HRKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> MYPTRW5ITVJ?
human A549 cell M1HVdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2j2bWlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlIxPiEQvF2= NVXjb3ZEW0GQR1XS
human HuP-T4 cell MoLpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHLZZ2pKdmirYnn0bY9vKG:oIHj1cYFvKEi3UD3UOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjR{N{Gg{txO M2X6dHNCVkeHUh?=
human HSC-2 cell NVjHe45PT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPOVodmUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNDd6NEKg{txO NEnVc5JUSU6JRWK=
human A253 cell NFHlUolIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXyTY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNlg{PCEQvF2= NED4[WlUSU6JRWK=
human HCT-116 cell MoLQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mkf2TY5pcWKrdHnvckBw\iCqdX3hckBRSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUiuNFA1OyEQvF2= NGTxe3BUSU6JRWK=
human HCT-116 cell NEm2fWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXPTY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVkvPjZ6IN88US=> NV[2R|FuW0GQR1XS

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID