Nocodazole

Catalog No.S2775 Synonyms: Oncodazole

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively.

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2 Customer Reviews

  • A, HeLa cells were treated with DMSO, Taxol (100 nM for 16 h), or Nocodazole (Noco, 100 ng/ml for 16 h). Total cell lysates were probed with the indicated antibodies against Hippo components on Phos-tag SDS-polyacrylamide gels. O and * mark the non-phosphorylated and phosphorylated proteins, respectively.

    J Biol Chem, 2016, 291:14761-14772.. Nocodazole purchased from Selleck.

    (B) HeLa cells were treated with DMSO, Taxol or Nocodazole (Noco). Total cell lysates were probed with the indicated antibodies on Phos-tag or regular SDS-polyacrylamide gels.

    Cell Signal, 2016, 28(12):1826-1832. Nocodazole purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells M1npN3Bzd2yrZnXyZZRqd25iYYPzZZk> MVi0PEBp NWPXZ5pGSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBz[XRiTGSxNkBk\WyuczDh[pRmeiB2ODDodpMh[nliWGTUJIF{e2G7LDDJR|UxRTZibl2= M{TjZ|E6OjJyMEG4
human PANC1 cells Mni3VJJwdGmoZYLheIlwdiCjc4PhfS=> Mm[0OFghcA>? MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCDTlOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OTBibl2= MX6yOVgzPzV{Mh?=
human HeLa cells Mlr4VJJwdGmoZYLheIlwdiCjc4PhfS=> NYj2b|JUPDhiaB?= MkD1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OTVibl2= NVHwSm1YOjV6Mke1NlI>
human MDA-MB-231 cells M1;YW3Bzd2yrZnXyZZRqd25iYYPzZZk> MlnaOFghcA>? M4nve2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVE4KG6P NVXhVFg4OjV6Mke1NlI>
human A549 cells M3vwTHBzd2yrZnXyZZRqd25iYYPzZZk> Mn3KOFghcA>? NGG2VHJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygS2k2OD1{MDDuUS=> NEHwfJEzPTh{N{WyNi=>
lymphoid leukemia L1210 cells M33JN3Bzd2yrZnXyZZRqd25iYYPzZZk> NWHsTVViPDhiaB?= M4jDe2lvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiY4XseJVz\WRibIntdIhwcWRibHX1b4VucWFiTEGyNVAh[2WubIOg[JVzcW6pIES4JIhzeyxiSVS1NF0zPyCwTR?= Mme3O|E{OTR6Mx?=
human A549 cells  M1fLbmN6fG:2b4jpZ:Kh[XO|YYm= MV6yOEBp NHSxOJNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9N|YvPiCwTR?= NV3pWXZGOjBzMUCxN|c>
L1210 murine leukemia cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGPkb|JKdiC4aYTyc{Bnd3JiaYTzJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBNOTJzMDDteZJqdmVibHX1b4VucWFiY3XscEBoem:5dHisJGlEPTB;M{igcm0> MnfwNVc{QDF2Nh?=
mouse P388 cells MYnQdo9tcW[ncnH0bY9vKGG|c3H5 MoTQOFghcA>? Mn76RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCSM{i4JINmdGy|IHHmeIVzKDR6IHjyd{BjgSC[VGSgZZN{[XluIFnDOVA:PDBibl2= MUOxPVIzODBzOB?=
LT12 cells NW\ITmE4WHKxbHnm[ZJifGmxbjDhd5NigQ>? MofuOFghcA>? MkfaRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDMWFEzKGOnbHzzJIJ6KFiWVDDhd5NigSCjZoTldkA1QCCqcoOsJGlEPTB;NECgcm0> MoLvNVcyQDFzNkS=
human U2OS cells NF;3NnBHfW6ldHnvckBie3OjeR?= MVK3NkBp M33tRmlv\HWldHnvckBw\iCvaYTveIlkKGG{cnXzeEBqdiCqdX3hckBWOk:VIHPlcIx{KGG|c3Xzd4VlKGG|IH3peI91cWNiaX7k[Zgh[W[2ZYKgO|IhcHK|IHL5JI1q[3Kxc3PvdIlkKGGwYXz5d4l{NCCHQ{WwQVUxKG6P NWTnO|ZKOjR7MEC4PFc>
human NCI-H460 cells MnXNVJJwdGmoZYLheIlwdiCjc4PhfS=> NWPud3hLPDhiaB?= M{PYfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[4JI5O MXmyNVU3Ozd3MB?=
human BT549 cells M{f0enBzd2yrZnXyZZRqd25iYYPzZZk> MoXXOFghcA>? M4[zbWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS1OFkh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTZ7IH7N Mny3NlE2PjN5NUC=
human 451LU cells NXSzPGtiWHKxbHnm[ZJifGmxbjDhd5NigQ>? MUi0PEBp NEn6e5FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIES1NWxWKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD15MjDuUS=> MXSyNVU3Ozd3MB?=
human SKOV3 cells M4PuO3Bzd2yrZnXyZZRqd25iYYPzZZk> NHfrWnU1QCCq MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONT2[zJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NF05OCCwTR?= NYPTZ2g4OjF3NkO3OVA>
K562 cells MVzGeY5kfGmxbjDhd5NigQ>? MUDF[oZm[3SrdnWgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDhdpJme3RicILvdIllcXWvIHnv[Ill\SC|dHHpcoVlKEt3NkKgZ4VtdHNiaX6gS|IwVSCyaHHz[UwhTUN3ME24NkBvVQ>? MXixNlg2Ojd4OB?=
human SKBR3 cells NXHxSlI6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn\wOFghcA>? M4foTWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHNMSlJ|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk> MlfRNlI5PTB{MUS=
human COLO205 cells MWTQdo9tcW[ncnH0bY9vKGG|c3H5 NF:xVXM1QCCq M1PPc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1;MU|IxPSClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmgMEBKSzVyPUi4JI5O NIWzS2kzOTV4M{e1NC=>
human KB/HeLa cells Mn7GSpVv[3Srb36gZZN{[Xl? MWeyOEBp M4fMfWNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGtDN0inTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgS|IwVSCyaHHz[UBi[2O3bYXsZZRqd25iYX\0[ZIhOjRiaILzJIJ6KE[DQ2OgZY5idHm|aYOsJGVEPTB;OUGgcm0> M{j3OVE6OjJyMEG4
human A549 cells M13pXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml\US5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gRVU1QSClZXzsd{BjgSCVdXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUCuNUDPxE1? M4DNbFIyOTB4NEW4
human MCF7 cells MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIJ6KFO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;MD6xJO69VQ>? NFKwTZczOTFyNkS1PC=>
human RKO cells M4S1RXBzd2yrZnXyZZRqd25iYYPzZZk> M2LVVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUlvPJINmdGy|IHL5JHhVXCCjc4PhfUwhUUN3ME2wMlEyKM7:TR?= NF\0ZlcyQTJ{MECxPC=>
human SW480 cells M4\6e3Bzd2yrZnXyZZRqd25iYYPzZZk> MnvIOFghcA>? MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZNEiwJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NF0xNjF{IN88US=> MoLxNlE2PjN5NUC=
human NCI-H460 cells NFPYZldRem:uaX\ldoF1cW:wIHHzd4F6 MlXLOFghcA>? MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGlEPTB;MD6xOUDPxE1? Mn25NlA2Ozd5NkW=
human SF268 cells MkfhR5l1d3SxeHnjxsBie3OjeR?= NF3O[HI1QCCq M33WTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNHOjZ6IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUC9NE4{KM7:TR?= MYCyNVcxPTJ{Mx?=
human SK-N-SH cells M{eySWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGXEb2FIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLWPIJINmdGy|IHL5JHN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OC54NjFOwG0> NYXh[XZnOjFzME[0OVg>
human MIAPaCa2 cells M2rtOXBzd2yrZnXyZZRqd25iYYPzZZk> M1HYeVQ5KGh? MUXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2LQWDhR4EzKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEeLNUC9NE46PSEQvF2= M{jIflI2OTNzOUW2
human HaCaT cells NYfhSYJwS3m2b4TvfIlkyqCjc4PhfS=> NH7DZ3E1QCCq NH\melREgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIZWNiXCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUiuPUDPxE1? MX[yOlI{OTB6MB?=
human SGC7901 cells NY\6UodzTnWwY4Tpc44h[XO|YYm= MV60PEBp NGDwSnJKdmS3Y4Tpc44hd2ZiZHnzeI9zfGmxboOgbY4hfHWkdXzpckBie3OnbXLsfUBqdiCqdX3hckBUT0N5OUCxJINmdGy|IHHzd4V{e2WmIHHzJIFjdm:{bXHsJI1qfG:2aXOgd5BqdmSuZTDmc5Ju[XSrb36gZZQhOiCob3zkJGlEPTBibHX2[YwhcW6ldXLheIVlKG[xcjC0PEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBucWO{b4Pjc5B6 MkezNlUzPjJyNUG=
human Jurkat T cells M3TaPWZ2dmO2aX;uJIF{e2G7 NUTybIFyOzZiaB?= MVTJcoR2[3Srb36gc4YhdWmlcn;0eYJ2dGViZHHtZYdmKGmwIHj1cYFvKEq3cnvheEBVKGOnbHzzJI93\XKneIDy[ZN{cW6pIFLjcFIh[W[2ZYKgN|YhcHK|IHL5JIludXWwb4P0ZYlvcW6pIH3leIhw\A>? NEPudY8zOjF7N{O5Ny=>
human PC3M cells MVfGeY5kfGmxbjDhd5NigQ>? MU[wMlUhfWdxbXy= NY\BcYp3OjRiaB?= NUfZW41pUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDQR|NOKGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEB{fWKJMTDwbIF{\SCjdDCwMlUhfWdxbXygZYZ1\XJiMkSgbJJ{KGK7IH\sc5ch[3m2b33leJJ6 MlHyNVgzPDd3N{O=
mouse NIH3T3 cells MlzvSpVv[3Srb36gZZN{[Xl? NF3zVGUxNjVidXevcYw> M4G2XVI1KGh? M{TwNGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gcY92e2ViTlnIN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEB{fWKJMTDwbIF{\SCjdDCwMlUhfWdxbXygZYZ1\XJiMkSgbJJ{KGK7IH\sc5ch[3m2b33leJJ6 NIjueYUyQDJ2N{W3Ny=>

... Click to View More Cell Line Experimental Data

In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:[5]
+ Expand
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Formulation: 0.05% dimethyls
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
Synonyms Oncodazole

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID