Nocodazole

Catalog No.S2775 Synonyms: Oncodazole

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively.

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2 Customer Reviews

  • A, HeLa cells were treated with DMSO, Taxol (100 nM for 16 h), or Nocodazole (Noco, 100 ng/ml for 16 h). Total cell lysates were probed with the indicated antibodies against Hippo components on Phos-tag SDS-polyacrylamide gels. O and * mark the non-phosphorylated and phosphorylated proteins, respectively.

    J Biol Chem, 2016, 291:14761-14772.. Nocodazole purchased from Selleck.

    (B) HeLa cells were treated with DMSO, Taxol or Nocodazole (Noco). Total cell lysates were probed with the indicated antibodies on Phos-tag or regular SDS-polyacrylamide gels.

    Cell Signal, 2016, 28(12):1826-1832. Nocodazole purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells MknXVJJwdGmoZYLheIlwdiCjc4PhfS=> NFnCcpY1QCCq MoPkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDyZZQhVFRzMjDj[YxteyCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwQVYhdk1? M1fiZlE6OjJyMEG4
human PANC1 cells MlXhVJJwdGmoZYLheIlwdiCjc4PhfS=> MlvGOFghcA>? MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCDTlOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OTBibl2= M2q4OlI2QDJ5NUKy
human HeLa cells NG\ENlJRem:uaX\ldoF1cW:wIHHzd4F6 MXe0PEBp NVfKbWxKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGdKPTB;MUWgcm0> MlzGNlU5Ojd3MkK=
human MDA-MB-231 cells Mn7ZVJJwdGmoZYLheIlwdiCjc4PhfS=> MoP4OFghcA>? M{jxXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVE4KG6P MUOyOVgzPzV{Mh?=
human A549 cells MWjQdo9tcW[ncnH0bY9vKGG|c3H5 NWGxSHJrPDhiaB?= MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0zOCCwTR?= M2i0PFI2QDJ5NUKy
lymphoid leukemia L1210 cells Mme5VJJwdGmoZYLheIlwdiCjc4PhfS=> NFq1fZU1QCCq NXLobZlpUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDjeYx1fXKnZDDsfY1xcG:rZDDs[ZVs\W2rYTDMNVIyOCClZXzsd{BlfXKrbnegOFghcHK|LDDJSFUxRTJ5IH7N MorGO|E{OTR6Mx?=
human A549 cells  MmOwR5l1d3SxeHnjxsBie3OjeR?= MVKyOEBp MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;M{[uOkBvVQ>? NH\UNYUzODFzMEGzOy=>
L1210 murine leukemia cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3zkOGlvKH[rdILvJIZweiCrdIOgbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGwyOjFyIH31dolv\SCuZYXr[Y1q[SClZXzsJIdzd3e2aDygTWM2OD1|ODDuUS=> M1zHUFE4OzhzNE[=
mouse P388 cells MknEVJJwdGmoZYLheIlwdiCjc4PhfS=> NFLDd|Q1QCCq NXPHeFlISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDQN|g5KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGlEPTB;NECgcm0> M2XlUFE6OjJyMEG4
LT12 cells NVPDXVJ1WHKxbHnm[ZJifGmxbjDhd5NigQ>? MXS0PEBp NGjyR5BCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGxVOTJiY3XscJMh[nliWGTUJIF{e2G7IHHmeIVzKDR6IHjyd{whUUN3ME20NEBvVQ>? NF71UZkyPzF6MUG2OC=>
human U2OS cells NV\0TGZ5TnWwY4Tpc44h[XO|YYm= MY[3NkBp NFTYZZJKdmS3Y4Tpc44hd2ZibXn0c5Rq[yCjcoLld5QhcW5iaIXtZY4hXTKRUzDj[YxteyCjc4Pld5Nm\CCjczDtbZRwfGmlIHnu[IV5KGGodHXyJFczKGi{czDifUBucWO{b4Pjc5Bq[yCjbnHsfZNqeyxiRVO1NF02OCCwTR?= NXu1cXI5OjR7MEC4PFc>
human NCI-H460 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 NEHiZpY1QCCq M4H5UGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[4JI5O MWeyNVU3Ozd3MB?=
human BT549 cells MWTQdo9tcW[ncnH0bY9vKGG|c3H5 MXG0PEBp NVPCN3hHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCWFU1QSClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;Nkmgcm0> MX2yNVU3Ozd3MB?=
human 451LU cells M3\vfnBzd2yrZnXyZZRqd25iYYPzZZk> MUO0PEBp M3;IWGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iNEWxUHUh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTd{IH7N NXPIVG1IOjF3NkO3OVA>
human SKOV3 cells MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 NGjObXU1QCCq NH\ZemhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLU3Y{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD16MDDuUS=> MmPwNlE2PjN5NUC=
K562 cells NF\5VW1HfW6ldHnvckBie3OjeR?= NE\qOnJG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BienKnc4SgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmWmIFu1OlIh[2WubIOgbY4hTzJxTTDwbIF{\SxiRVO1NF05OiCwTR?= MnW0NVI5PTJ5Nki=
human SKBR3 cells NGO1TW5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEf3NoY1QCCq MX7Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTT2JTOyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5 NXPRO5FjOjJ6NUCyNVQ>
human COLO205 cells NXPHRWNHWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVG0PEBp MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEORTF:yNFUh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7IDygTWM2OD16ODDuUS=> M1fyRVIyPTZ|N{Ww
human KB/HeLa cells NFzHSmhHfW6ldHnvckBie3OjeR?= MVSyOEBp NY\kRnUyS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hU0JxSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BIOi:PIIDoZZNmKGGlY4XteYxifGmxbjDh[pRmeiB{NDDodpMh[nliRlHDV{BidmGueYPpd{whTUN3ME25NUBvVQ>? NUHue|dsOTl{MkCwNVg>
human A549 cells M{KxdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1TOe2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGE2PDliY3XscJMh[nliU4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhT0l3ME2wMlEh|ryP MomwNlEyODZ2NUi=
human MCF7 cells MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGHwVG1Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIx{KGK7IGP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9NE4yKM7:TR?= NV\VdXFiOjFzME[0OVg>
human RKO cells NVLxS29OWHKxbHnm[ZJifGmxbjDhd5NigQ>? MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyCkeTDYWHQh[XO|YYmsJGlEPTB;MD6xNUDPxE1? MkG3NVkzOjByMUi=
human SW480 cells M2PjRXBzd2yrZnXyZZRqd25iYYPzZZk> M1fIPVQ5KGh? NWrCZ4J5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTW|Q5OCClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;MD6xNkDPxE1? MoPkNlE2PjN5NUC=
human NCI-H460 cells MkTjVJJwdGmoZYLheIlwdiCjc4PhfS=> NVvXb5hzPDhiaB?= NXfU[|B7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwQVAvOTVizszN NHXkN|MzODV|N{e2OS=>
human SF268 cells Mm[2R5l1d3SxeHnjxsBie3OjeR?= M4fIelQ5KGh? MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTSlI3QCClZXzsd{Bi\nSncjC0PEBpenNiYomgXHRVKGG|c3H5MEBKSzVyPUCuN{DPxE1? NHXoSlQzOTdyNUKyNy=>
human SK-N-SH cells MkSyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NG\STWRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLWPIJINmdGy|IHL5JHN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OC54NjFOwG0> MYiyNVExPjR3OB?=
human MIAPaCa2 cells MmDqVJJwdGmoZYLheIlwdiCjc4PhfS=> MUW0PEBp NI\EeWlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3JRXBiS2F{IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OC57NTFOwG0> MViyOVE{OTl3Nh?=
human HaCaT cells MYXDfZRwfG:6aXRCpIF{e2G7 M4jUT|Q5KGh? MnW1R5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHHDZXQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwh\3Kxd4ToJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD16Lkmg{txO M3P2eFI3OjNzMEiw
human SGC7901 cells M2L5OWZ2dmO2aX;uJIF{e2G7 NYLxPVNPPDhiaB?= NHXLc3pKdmS3Y4Tpc44hd2ZiZHnzeI9zfGmxboOgbY4hfHWkdXzpckBie3OnbXLsfUBqdiCqdX3hckBUT0N5OUCxJINmdGy|IHHzd4V{e2WmIHHzJIFjdm:{bXHsJI1qfG:2aXOgd5BqdmSuZTDmc5Ju[XSrb36gZZQhOiCob3zkJGlEPTBibHX2[YwhcW6ldXLheIVlKG[xcjC0PEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBucWO{b4Pjc5B6 MoLkNlUzPjJyNUG=
human Jurkat T cells MX;GeY5kfGmxbjDhd5NigQ>? MYSzOkBp Mn;iTY5lfWO2aX;uJI9nKG2rY4LveJVjfWynIHThcYFo\SCrbjDoeY1idiCMdYLrZZQhXCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDCZ4wzKGGodHXyJFM3KGi{czDifUBqdW23bn;zeIFqdmmwZzDt[ZRpd2R? MmWzNlIyQTd|OUO=
human PC3M cells M{XsUWZ2dmO2aX;uJIF{e2G7 NV3wRZc2OC53IIXnM41t MYqyOEBp M{LXe2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iUFOzUUBk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25iYYSgd5VjTzFicHjhd4Uh[XRiMD61JJVoN22uIHHmeIVzKDJ2IHjyd{BjgSCobH;3JIN6fG:vZYTyfS=> MWCxPFI1PzV5Mx?=
mouse NIH3T3 cells M3iwXWZ2dmO2aX;uJIF{e2G7 NYGyRnpZOC53IIXnM41t MXqyOEBp MknMTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SCQSVizWFMh[2WubIOgZZN{\XO|ZXSgZZMh[WOldX31cIF1cW:wIHH0JJN2[kdzIIDoZZNmKGG2IECuOUB2\y:vbDDh[pRmeiB{NDDodpMh[nliZnzve{BkgXSxbXX0dpk> NUCwVW9qOTh{NEe1O|M>

... Click to View More Cell Line Experimental Data

In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:[5]
+ Expand
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Formulation: 0.05% dimethyls
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
Synonyms Oncodazole

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID