Nocodazole

Catalog No.S2775 Synonyms: Oncodazole

Nocodazole Chemical Structure

Molecular Weight(MW): 301.32

Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.

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2 Customer Reviews

  • A, HeLa cells were treated with DMSO, Taxol (100 nM for 16 h), or Nocodazole (Noco, 100 ng/ml for 16 h). Total cell lysates were probed with the indicated antibodies against Hippo components on Phos-tag SDS-polyacrylamide gels. O and * mark the non-phosphorylated and phosphorylated proteins, respectively.

    J Biol Chem, 2016, 291:14761-14772.. Nocodazole purchased from Selleck.

    (B) HeLa cells were treated with DMSO, Taxol or Nocodazole (Noco). Total cell lysates were probed with the indicated antibodies on Phos-tag or regular SDS-polyacrylamide gels.

    Cell Signal, 2016, 28(12):1826-1832. Nocodazole purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Nocodazole is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.
Targets
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
In vitro

Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. [1] At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. [2] Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. [3] Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. [4]Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing[6].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat LT12 cells MWTQdo9tcW[ncnH0bY9vKGG|c3H5 NGLqXnE1QCCq NH;oU4lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JJJifCCOVEGyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSC[VGSgZZN{[XluIFnDOVA:PiCwTR?= MVSxPVIzODBzOB?=
human PANC1 cells MWHQdo9tcW[ncnH0bY9vKGG|c3H5 NX\0W2xbPDhiaB?= NGjPPJdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDBUmMyKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGdKPTB;MUCgcm0> MUWyOVgzPzV{Mh?=
human HeLa cells MYHQdo9tcW[ncnH0bY9vKGG|c3H5 NEHXdW01QCCq NITIeoxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygS2k2OD1zNTDuUS=> MWSyOVgzPzV{Mh?=
human MDA-MB-231 cells NXfqTnR[WHKxbHnm[ZJifGmxbjDhd5NigQ>? MYK0PEBp M1LyV2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVE4KG6P MWWyOVgzPzV{Mh?=
human A549 cells NEj1W5VRem:uaX\ldoF1cW:wIHHzd4F6 NXHGdWw2PDhiaB?= M{O0dWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTJyIH7N M4Dqb|I2QDJ5NUKy
lymphoid leukemia L1210 cells MUHQdo9tcW[ncnH0bY9vKGG|c3H5 NWSzUoFvPDhiaB?= NYjFNHBjUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDjeYx1fXKnZDDsfY1xcG:rZDDs[ZVs\W2rYTDMNVIyOCClZXzsd{BlfXKrbnegOFghcHK|LDDJSFUxRTJ5IH7N NXfKcW06PzF|MUS4Ny=>
human A549 cells  NUHrbVJmS3m2b4TvfIlkyqCjc4PhfS=> Ml7qNlQhcA>? M3qyNGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zOk43KG6P M3T3XlIxOTFyMUO3
L1210 murine leukemia cell NH;nXnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX;FWpNGUW5idnn0do8h\m:{IHn0d{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhVDF{MUCgcZVzcW6nIHzleYtmdWmjIHPlcIwh\3Kxd4ToMEBKSzVyPUO4JI5O NXfNOHVUOTd|OEG0Oi=>
mouse P388 cells NE\EcZpRem:uaX\ldoF1cW:wIHHzd4F6 Mn;TOFghcA>? NWjhPJdESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDQN|g5KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGlEPTB;NECgcm0> MnfUNVkzOjByMUi=
LT12 cells MUHQdo9tcW[ncnH0bY9vKGG|c3H5 MVO0PEBp NVjodplQSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBNXDF{IHPlcIx{KGK7IGjUWEBie3OjeTDh[pRmeiB2ODDodpMtKEmFNUC9OFAhdk1? MnjKNVcyQDFzNkS=
human U2OS cells NXz6b|VCTnWwY4Tpc44h[XO|YYm= MXe3NkBp MmKwTY5lfWO2aX;uJI9nKG2rdH;0bYMh[XK{ZYP0JIlvKGi3bXHuJHUzV1NiY3XscJMh[XO|ZYPz[YQh[XNibXn0c5Rq[yCrbnTlfEBi\nSncjC3NkBpenNiYomgcYlkem:|Y3;wbYMh[W6jbInzbZMtKEWFNUC9OVAhdk1? NFzhOZUzPDlyMEi4Oy=>
human NCI-H460 cells MVXQdo9tcW[ncnH0bY9vKGG|c3H5 MVi0PEBp MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD14ODDuUS=> M1O0N|IyPTZ|N{Ww
human BT549 cells NWXIR4xrWHKxbHnm[ZJifGmxbjDhd5NigQ>? MUm0PEBp MmHNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVEW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVA:Pjlibl2= MYeyNVU3Ozd3MB?=
human 451LU cells Mnn2VJJwdGmoZYLheIlwdiCjc4PhfS=> M3HiRlQ5KGh? MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKDR3MVzVJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NF04OiCwTR?= NHXzSG0zOTV4M{e1NC=>
human SKOV3 cells MlHYVJJwdGmoZYLheIlwdiCjc4PhfS=> M4DSOFQ5KGh? NYfYV3ltSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{Bi\nSncjC0PEBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;OECgcm0> Mke2NlE2PjN5NUC=
K562 cells MlLXSpVv[3Srb36gZZN{[Xl? Mm\xSYZn\WO2aY\lJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gZZJz\XO2IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5m\CCNNU[yJINmdGy|IHnuJGczN01icHjhd4UtKEWFNUC9PFIhdk1? NH3Ld3IyOjh3Mke2PC=>
human SKBR3 cells NXX0XI1vT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXzR[plGPDhiaB?= MkP1S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV2tDWjNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfS=> M2LGN|IzQDVyMkG0
human COLO205 cells MoDIVJJwdGmoZYLheIlwdiCjc4PhfS=> MXe0PEBp MkDVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFT1zPNlA2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTCsJGlEPTB;OEigcm0> MXOyNVU3Ozd3MB?=
human KB/HeLa cells MlLMSpVv[3Srb36gZZN{[Xl? NXLYd3B2OjRiaB?= NUT6OoZlS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hU0JxSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BIOi:PIIDoZZNmKGGlY4XteYxifGmxbjDh[pRmeiB{NDDodpMh[nliRlHDV{BidmGueYPpd{whTUN3ME25NUBvVQ>? NV7hSFRYOTl{MkCwNVg>
human A549 cells NIrh[JVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLHNlV3T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxteyCkeTDTeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvOSEQvF2= MWWyNVExPjR3OB?=
human MCF7 cells NE\V[|VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NELSd49Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIx{KGK7IGP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9NE4yKM7:TR?= MUeyNVExPjR3OB?=
human RKO cells NVPVPXdMWHKxbHnm[ZJifGmxbjDhd5NigQ>? MYjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyCkeTDYWHQh[XO|YYmsJGlEPTB;MD6xNUDPxE1? NFrHOY8yQTJ{MECxPC=>
human SW480 cells Ml\qVJJwdGmoZYLheIlwdiCjc4PhfS=> NEPvdI01QCCq MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZNEiwJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NF0xNjF{IN88US=> MYWyNVU3Ozd3MB?=
human NCI-H460 cells MmTjVJJwdGmoZYLheIlwdiCjc4PhfS=> MYC0PEBp M1SzUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHhVXCCjc4PhfUwhUUN3ME2wMlE2KM7:TR?= NUW4XWQ{OjB3M{e3OlU>
human SF268 cells MYjDfZRwfG:6aXRCpIF{e2G7 MlTiOFghcA>? M3exdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNHOjZ6IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUC9NE4{KM7:TR?= MV6yNVcxPTJ{Mx?=
human SK-N-SH cells MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NET5fmJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLWPIJINmdGy|IHL5JHN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OC54NjFOwG0> M1LVflIyOTB4NEW4
human MIAPaCa2 cells MonIVJJwdGmoZYLheIlwdiCjc4PhfS=> NVnG[mhVPDhiaB?= M{OydmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVnBVIFE[TJiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjl3IN88US=> NHPDdpUzPTF|MUm1Oi=>
human HaCaT cells MYPDfZRwfG:6aXRCpIF{e2G7 NYCydmhZPDhiaB?= NH2z[ZpEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIZWNiXCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUiuPUDPxE1? MUGyOlI{OTB6MB?=
human SGC7901 cells M3HqbmZ2dmO2aX;uJIF{e2G7 MWi0PEBp MnnWTY5lfWO2aX;uJI9nKGSrc4TvdpRqd26|IHnuJJR2[nWuaX6gZZN{\W2kbImgbY4hcHWvYX6gV2dEPzlyMTDj[YxteyCjc4Pld5Nm\CCjczDhZo5wem2jbDDtbZRwfGmlIIPwbY5ldGViZn;ycYF1cW:wIHH0JFIh\m:uZDDJR|UxKGyndnXsJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgcYlkem:|Y3;wfS=> M3HsZlI2OjZ{MEWx
human Jurkat T cells NVq1dGJpTnWwY4Tpc44h[XO|YYm= NFfXZYo{PiCq MVrJcoR2[3Srb36gc4YhdWmlcn;0eYJ2dGViZHHtZYdmKGmwIHj1cYFvKEq3cnvheEBVKGOnbHzzJI93\XKneIDy[ZN{cW6pIFLjcFIh[W[2ZYKgN|YhcHK|IHL5JIludXWwb4P0ZYlvcW6pIH3leIhw\A>? MmjVNlIyQTd|OUO=
human PC3M cells MVHGeY5kfGmxbjDhd5NigQ>? NIL4WHIxNjVidXevcYw> MY[yOEBp MYPJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKFCFM12gZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IIP1ZmcyKHCqYYPlJIF1KDBwNTD1[{9udCCjZoTldkAzPCCqcoOgZpkh\myxdzDjfZRwdWW2com= NFqwTFgyQDJ2N{W3Ny=>
mouse NIH3T3 cells NXjodHNETnWwY4Tpc44h[XO|YYm= MnW1NE42KHWpL33s MYCyOEBp M4Tvdmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gcY92e2ViTlnIN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEB{fWKJMTDwbIF{\SCjdDCwMlUhfWdxbXygZYZ1\XJiMkSgbJJ{KGK7IH\sc5ch[3m2b33leJJ6 MmLqNVgzPDd3N{O=

... Click to View More Cell Line Experimental Data

In vivo The tumor volume and tumor weight of the mice treated with Ketoconazole  plus  Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole  plus  Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole  or  Nocodazole alone. [5]

Protocol

Animal Research:

[5]

+ Expand
  • Animal Models: Nude mice with COLO-205 tumor xenografts
  • Formulation: 0.05% dimethyls
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 301.32
Formula

C14H11N3O3S

CAS No. 31430-18-9
Storage powder
in solvent
Synonyms Oncodazole

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Microtubule Associated Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID