Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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3 Customer Reviews

  • Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell NU\uOmV5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGjqc|BKdmirYnn0bY9vKG:oIHj1cYFvKEyFLUKtZYQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Njd5ZT2wOkDPxE1? NHO0PHBUSU6JRWK=
human A431 cell M1LoZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MofHTY5pcWKrdHnvckBw\iCqdX3hckBCPDNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT64OoUuODZizszN MVXTRW5ITVJ?
human SW1710 cell MnLMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFHDT2NKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOTSnLUC1JO69VQ>? NFnNR|hUSU6JRWK=
human Daoy cell NFrxe2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHXRdnhKdmirYnn0bY9vKG:oIHj1cYFvKESjb4mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVIvPzOnLUC1JO69VQ>? M2XZZnNCVkeHUh?=
human BFTC-905 cell M1S0T2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE46QWVvMEWg{txO NI\qUGdUSU6JRWK=
human A375 cell NED6RVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVPRbplPUW6qaXLpeIlwdiCxZjDoeY1idiCDM{e1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OYUuODVizszN MlLOV2FPT0WU
human RKO cell MoqzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYLGU3ppUW6qaXLpeIlwdiCxZjDoeY1idiCUS1:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlQ4\S1yNTFOwG0> Mn36V2FPT0WU
human KYSE-510 cell NGfmUZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3XsNGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT21NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjN6ZT2wOUDPxE1? MmDoV2FPT0WU
human HLE cell NInJbmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH:5[5pKdmirYnn0bY9vKG:oIHj1cYFvKEiORTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlZmNTB3IN88US=> MnX5V2FPT0WU
human MCF7 cell NHG2e2dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mni1TY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD62OoUuODVizszN M4[yTHNCVkeHUh?=
human 786-0 cell MkK4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF\4To9KdmirYnn0bY9vKG:oIHj1cYFvKDd6Nj2wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOSCwTR?= NWDlOWVJW0GQR1XS
human TE-8 cell MnrSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFTnUJRKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlEyOSCwTR?= MUHTRW5ITVJ?
ovarian carcinoma 1A9 cell M1uwNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlzZTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3Rib4\hdolidiClYYLjbY5wdWFiMVG5JINmdGxiZ4Lve5Rp NIi4VHUyPjF|NEmyPC=>
human PANC-03-27 cell M1jKO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnjhTY5pcWKrdHnvckBw\iCqdX3hckBRSU6FLUCzMVI4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{Kg{txO NHWw[FRUSU6JRWK=
human VMRC-RCZ cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH6xUIlKdmirYnn0bY9vKG:oIHj1cYFvKF[PUlOtVmNbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zM{[g{txO MkHUV2FPT0WU
human IGROV-1 cell NWTHNIpwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVPIXXNUUW6qaXLpeIlwdiCxZjDoeY1idiCLR2LPWk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNU[g{txO NGHJbHhUSU6JRWK=
human MG-63 cell NWDn[HBDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJG1INTZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOlUh|ryP MVfTRW5ITVJ?
human SIG-M5 cell NWPK[Ic2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUPvRlY1UW6qaXLpeIlwdiCxZjDoeY1idiCVSVetUVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF4OTFOwG0> NV\5RodjW0GQR1XS
human NCI-H650 cell NWP2Zmt6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NI\zNGdKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOlUxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNzFOwG0> NGq2OWJUSU6JRWK=
human PSN1 cell NGntRnlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFf5WW9KdmirYnn0bY9vKG:oIHj1cYFvKFCVTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE4OyEQvF2= NWDyd4NUW0GQR1XS
human PA-1 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEXN[JVKdmirYnn0bY9vKG:oIHj1cYFvKFCDLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE4PCEQvF2= MoXOV2FPT0WU
KB-8511 cells MmTTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mm\0O|IhcA>? M1XHTWNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDD0bIUh\3Kxd4ToJI9nKHCjY3zpeIF5\WxvcnXzbZN1[W62IHj1cYFvKGWyaXTldo1wcWRiY3HyZ4lvd22jIHPlcIx{KEuELUi1NVEh[nliNUCgdIVz[2WwdDCoO|IhcHJiZYjwc5N2emVrLDDJR|UxRTBwMUig{txO MXKxNVE{OzB6Nh?=
KB-31 cells NFi4cHZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLJZ2Q4PzJiaB?= NVXx[ZV1S2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJRp\SCpcn;3eIghd2ZicHHjcIl1[XinbD3z[Y5{cXSrdnWgbJVu[W5iZYDp[IVzdW:rZDDjZZJkcW6xbXGgZ4VtdHNiS1KtN|Eh[nliNUCgdIVz[2WwdDCoO|IhcHJiZYjwc5N2emVr MmL5NVEyOzNyOE[=
human BHY cell Mny3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3K2OGlvcGmkaYTpc44hd2ZiaIXtZY4hSki\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xPVUhdk1? MWLTRW5ITVJ?
human BB30-HNC cell NY\PW49TT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{nLSWlvcGmkaYTpc44hd2ZiaIXtZY4hSkJ|MD3IUmMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJyMTFOwG0> MYDTRW5ITVJ?
human HOS cell M1u2bmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXv3UGJ{UW6qaXLpeIlwdiCxZjDoeY1idiCKT2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIyPSEQvF2= MoPuV2FPT0WU
human 639-V cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIPJUY1KdmirYnn0bY9vKG:oIHj1cYFvKDZ|OT3WJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOThizszN NFrCeWpUSU6JRWK=
human ES1 cell  NXPY[YlpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3G1N2lvcGmkaYTpc44hd2ZiaIXtZY4hTVNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlEh|ryP MlPnV2FPT0WU
human TE-15 cell  NGLVc5lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWjJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlUh|ryP NV\GNWh7W0GQR1XS
human LXF-289 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEn2VFBKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ5NzFOwG0> NInkTm5USU6JRWK=
human CAL-12T cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXy5cHBGUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNVJVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{M{mg{txO M3q5UnNCVkeHUh?=
human T84 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4O5fmlvcGmkaYTpc44hd2ZiaIXtZY4hXDh2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yOEDPxE1? M{DhWnNCVkeHUh?=
human KYSE-450 cell MlzrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml;BTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUS1NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjZ5IN88US=> M2L2OnNCVkeHUh?=
human LCLC-103H cell NXv2U|NNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIPybYFKdmirYnn0bY9vKG:oIHj1cYFvKEyFTFOtNVA{UCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwM{Ggcm0> NX;0XmZiW0GQR1XS
human NCI-H810 cell M3Sy[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2TxOmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi4NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{MjFOwG0> NVrGRWtZW0GQR1XS
human HUTU-80 cell M{jFbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4m2WGlvcGmkaYTpc44hd2ZiaIXtZY4hUFWWVT24NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzJ6IN88US=> NXTr[YpoW0GQR1XS
human NCI-H460 cell NXLZRWlkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4LidmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN|MzFOwG0> M{G4NnNCVkeHUh?=
human HGC-27 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3e2TmlvcGmkaYTpc44hd2ZiaIXtZY4hUEeFLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{OzhizszN Mm\rV2FPT0WU
human HSC-2 cell NGK2dY1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXvDeItrUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzZ|IH7N NHft[2pUSU6JRWK=
human IA-LM cell NWTZN4V1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEPNTFdKdmirYnn0bY9vKG:oIHj1cYFvKEmDLVzNJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{QTNizszN MmLJV2FPT0WU
human HMV-II cell NVn1W2MxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGhOXi2LSTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuN|k1KM7:TR?= MVLTRW5ITVJ?
human COLO-679 cell Mon4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFfVOYNKdmirYnn0bY9vKG:oIHj1cYFvKEORTF:tOlc6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|OUSg{txO M2H0VXNCVkeHUh?=
human NCI-H2122 cell MmGyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUjJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkGyNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDFizszN NY\VeWtKW0GQR1XS
human CAL-39 cell NHO4eIhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnNTY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQyOSEQvF2= M1\tRnNCVkeHUh?=
human SW954 cell NFjjd5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYrJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTV2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60N|Yh|ryP NI\sUXlUSU6JRWK=
human LCLC-97TM1 cell MorsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWrhcYRuUW6qaXLpeIlwdiCxZjDoeY1idiCOQ1zDMVk4XE1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60OFQh|ryP NEOz[FVUSU6JRWK=
human KYSE-270 cell M37HV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV;lVGFyUW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNES1JO69VQ>? MVXTRW5ITVJ?
human NB13 cell MmX1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkTrTY5pcWKrdHnvckBw\iCqdX3hckBPSjF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60OFch|ryP MlftV2FPT0WU
human SK-LMS-1 cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXHabVR6UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNkS2JO69VQ>? Mn7OV2FPT0WU
human SW620 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHvSSHZKdmirYnn0bY9vKG:oIHj1cYFvKFOZNkKwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE43PDhizszN MmrTV2FPT0WU
human HCT-15 cell NIfjfG5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXHJcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|E4KM7:TR?= NU\mVWl7W0GQR1XS
human DU-145 cell M2r1U2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1PyRmlvcGmkaYTpc44hd2ZiaIXtZY4hTFVvMUS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44OjFizszN Mlz0V2FPT0WU
human ST486 cell NFvxSFNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M33SZWlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlczOSEQvF2= MkTOV2FPT0WU
human A427 cell MnXhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGnKPZlKdmirYnn0bY9vKG:oIHj1cYFvKEF2MkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlgzQCEQvF2= MX\TRW5ITVJ?
human HT-1080 cell NIG1eHpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHXnXXFKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUGwPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh2MjFOwG0> NV\US2FyW0GQR1XS
human NCI-H2009 cell NEnZcYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFHVVYtKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlAxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOE[yJO69VQ>? NUL6RpJVW0GQR1XS
human SK-LU-1 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWXRVFRkUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56Nk[g{txO MojUV2FPT0WU
human SCC-4 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGrmZlhKdmirYnn0bY9vKG:oIHj1cYFvKFOFQz20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PzdizszN NH\rS5FUSU6JRWK=
human NCI-H1299 cell Mn3xS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGDV[41KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVI6QSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOEig{txO MYfTRW5ITVJ?
human NH-12 cell MlXIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWjJcohq[mm2aX;uJI9nKGi3bXHuJG5JNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64PVEh|ryP M2PTXnNCVkeHUh?=
human SK-UT-1 cell NHnWSpdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1W5eGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvVWStNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDl{IN88US=> MVHTRW5ITVJ?
human Bel7402 MonFVJJwdGmoZYLheIlwdiCjc4PhfS=> MoTrO|IhcA>? NEnCOnpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLlcFc1ODJiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmeiCJbH:gZZN{[XluIFnDOVA:OC57IN88US=> NFrKbZYzOjN{MEO1OC=>
human A388 cell M2fHPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH\PNYFKdmirYnn0bY9vKG:oIHj1cYFvKEF|OEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlk1KM7:TR?= M4raO3NCVkeHUh?=
human SW982 cell NF\3dW1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnHBTY5pcWKrdHnvckBw\iCqdX3hckBUXzl6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVk6KM7:TR?= MUHTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
+ Expand

Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
+ Expand
  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water slightly soluble or insoluble
In vivo Add solvents individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers, The State University of New Jersey July 2007 Phase 1
NCT00468260 Terminated Advanced Malignancies Novartis Pharmaceuticals|Novartis May 2007 Phase 1
NCT00448396 Completed Advanced Malignancies Novartis Pharmaceuticals|Novartis March 2007 Phase 1
NCT00407251 Completed Hormone Refractory Prostate Cancer British Columbia Cancer Agency February 2007 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Microtubule Associated Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID