Patupilone (EPO906, Epothilone B)

Catalog No.S1364

Patupilone (EPO906, Epothilone B) Chemical Structure

Molecular Weight(MW): 507.68

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.

Size Price Stock Quantity  
In DMSO USD 250 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
USD 470 In stock
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3 Customer Reviews

  • Matrigel invasion assay of H1299 cells treated with SDF-1α or SDF-1α plus Epothilone B prior to 0 or 2 Gy irradiation. After the treatment for 12 h, the H1299 cells that migrated to the bottom surface of the membrane were stained with Giemsa, and the number of migrated cells was calculated manually.

    Oncotarget, 2015, 6(13):10893-907.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

    Combination treatment with epothilone B and ABT-737 inhibited PI3K/AKT/mTOR pathway. SKOV-3 cells were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination, OVCAR-8 cells were treated with epothilone B (0.3 nM), ABT-737 (3 μM), or the combination, SGC-7901 were treated with epothilone B (0.4 nM), ABT-737 (5 μM), or the combination. Cells were exposed to compounds for 48 h, after which protein extracts were immunoblotted with specified antibodies for p-4E-BP-1, p-mTOR, p-AKT, caspase-3, PARP and β-actin.

    J Cancer Res Clin Oncol, 2016, 142(11):2281-9.. Patupilone (EPO906, Epothilone B) purchased from Selleck.

  • Cells were seeded in 96 well paltes, and then treated with the indicated concentration of Epothilone B for 48 h. Cell survival was measured by a standarad MTT assay.

     

     

    Dr. Helen Sadik of Johns Hopkins University. Patupilone (EPO906, Epothilone B) purchased from Selleck.

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC0.01 of 1.8 μM. Phase 2.
Targets
Tubulin [1]
(Cell-free assay)
1.8 μM(EC0.01)
In vitro

Epothilone B shows better activity than Epothilone A. The EC0.01 of Epothilone B is 1.8 μM. Epothilone B potently inhibits cell proliferation in HCT116 cells, with IC50 of 0.8 nM. [1] Epothilone B induces mitotic arrest and displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 of 3 nM to 92 nM. Epothilone B competes with Taxol in binding to microtubules, with IC50 of 3.3 μM. [2] In MCF-7 cells overexpressing GFP-α-tubulin, Epothilone B (3.5 nM) efficiently blocks microtubule dynamics. Meanwhile, Epothilone B induces mitotic arrest with IC50 of 3.5 nM. [3] In multiple myeloma (MM) cells, including RPMI 8226, U266, MM.1S, LR5, and MR20, Epothilone B directly suppresses proliferation with IC50 of 1 nM to 10 nM. Similarly, Epothilone B (10 nM) also induces cell cycle arrest and apoptosis. [4] A recent study reveals that, in ovarian cancer Hey cells, Epothilone B (5 nM–100 nM) enhances surface epithelial cell adhesion antigen (EpCAM), without affecting the transcription or the total cellular level of EpCAM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LC-2-ad cell NVW3e2M6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1HRXGlvcGmkaYTpc44hd2ZiaIXtZY4hVENvMj3h[EBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPzenLUC2JO69VQ>? MkfuV2FPT0WU
human A431 cell Mny0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWnJcohq[mm2aX;uJI9nKGi3bXHuJGE1OzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17Lki2[U0xPiEQvF2= MWHTRW5ITVJ?
human SW1710 cell MontS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mmj4TY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlE1\S1yNTFOwG0> NXX3Ro9qW0GQR1XS
human Daoy cell NF;0XHVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXnFbpVJUW6qaXLpeIlwdiCxZjDoeY1idiCGYX;5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0zNjd|ZT2wOUDPxE1? MXHTRW5ITVJ?
human BFTC-905 cell NF3yc3VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn70TY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvQTmnLUC1JO69VQ>? Mme4V2FPT0WU
human A375 cell NX3pdFFPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHnzTYZKdmirYnn0bY9vKG:oIHj1cYFvKEF|N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21[U0xPSEQvF2= NVzuW29LW0GQR1XS
human RKO cell MnTvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHXlcolKdmirYnn0bY9vKG:oIHj1cYFvKFKNTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uOFdmNTB3IN88US=> M{XpVnNCVkeHUh?=
human KYSE-510 cell M1zxOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3LUUGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT21NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjN6ZT2wOUDPxE1? MlHXV2FPT0WU
human HLE cell M1LQe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVrJcohq[mm2aX;uJI9nKGi3bXHuJGhNTSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwMk\lMVA2KM7:TR?= NFKxNYlUSU6JRWK=
human MCF7 cell M{n2Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml;YTY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD62OoUuODVizszN MmH4V2FPT0WU
human 786-0 cell NYLCdFZ[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3nHbmlvcGmkaYTpc44hd2ZiaIXtZY4hPzh4LUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlEyKG6P NFLoVlNUSU6JRWK=
human TE-8 cell NVj1OFRbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnjaTY5pcWKrdHnvckBw\iCqdX3hckBVTS16IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xNVEhdk1? M4LRZXNCVkeHUh?=
ovarian carcinoma 1A9 cell NV72VZZuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4fwdGlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IH;2ZZJq[W5iY3HyZ4lvd22jIEHBPUBk\WyuIHfyc5d1cA>? MlTYNVYyOzR7Mki=
human PANC-03-27 cell M3vYcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWDPWWpIUW6qaXLpeIlwdiCxZjDoeY1idiCSQV7DMVA{NTJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xN|Ih|ryP MmDzV2FPT0WU
human VMRC-RCZ cell NE\3SXRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkHkTY5pcWKrdHnvckBw\iCqdX3hckBXVVKFLWLDXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTN4IN88US=> M3v2Z3NCVkeHUh?=
human IGROV-1 cell M2HOUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGPlfYRKdmirYnn0bY9vKG:oIHj1cYFvKEmJUl;WMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF3NjFOwG0> NX;sS4VIW0GQR1XS
human MG-63 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUPJcohq[mm2aX;uJI9nKGi3bXHuJG1INTZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOlUh|ryP M{HJW3NCVkeHUh?=
human SIG-M5 cell NFLZPVNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkDmTY5pcWKrdHnvckBw\iCqdX3hckBUUUdvTUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE3QSEQvF2= NX25Wpp1W0GQR1XS
human NCI-H650 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4\pdmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi2OVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjF5IN88US=> MlXQV2FPT0WU
human PSN1 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3XH[2lvcGmkaYTpc44hd2ZiaIXtZY4hWFOQMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVc{KM7:TR?= MXnTRW5ITVJ?
human PA-1 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXfJcohq[mm2aX;uJI9nKGi3bXHuJHBCNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG3OEDPxE1? NVjvPWNvW0GQR1XS
KB-8511 cells MofNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnezO|IhcA>? NHHEU49Ed26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgeIhmKGe{b4f0bEBw\iCyYXPsbZRigGWuLYLld4l{fGGwdDDoeY1idiCncHnk[ZJud2mmIHPhdoNqdm:vYTDj[YxteyCNQj24OVEyKGK7IEWwJJBmemOnboSgLFczKGi{IHX4dI9{fXKnKTygTWM2OD1yLkG4JO69VQ>? Mm\aNVEyOzNyOE[=
KB-31 cells MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NES0dII4OiCq MmO1R49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IITo[UBoem:5dHigc4YheGGlbHn0ZZhmdC2|ZX7zbZRqfmViaIXtZY4h\XCrZHXycY9q\CClYYLjbY5wdWFiY3XscJMhU0JvM{GgZpkhPTBicHXyZ4VvfCBqN{KgbJIh\Xiyb4P1doUq NIX3eFEyOTF|M{C4Oi=>
human BHY cell MlzUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXjQSIVqUW6qaXLpeIlwdiCxZjDoeY1idiCESGmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlE6PSCwTR?= M4XwcHNCVkeHUh?=
human BB30-HNC cell MmC5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXH1eGVOUW6qaXLpeIlwdiCxZjDoeY1idiCEQkOwMWhPSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkCxJO69VQ>? NYPuO2d2W0GQR1XS
human HOS cell Mo[wS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk\uTY5pcWKrdHnvckBw\iCqdX3hckBJV1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKxOUDPxE1? M4\GPHNCVkeHUh?=
human 639-V cell MlfES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWfUeYh6UW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjF6IN88US=> M33WRXNCVkeHUh?=
human ES1 cell  NIPwVHpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYnM[YVCUW6qaXLpeIlwdiCxZjDoeY1idiCHU{GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIzOSEQvF2= NUHWeIlWW0GQR1XS
human TE-15 cell  NWjJVJNpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mkf6TY5pcWKrdHnvckBw\iCqdX3hckBVTS1zNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlI2KM7:TR?= MXPTRW5ITVJ?
human LXF-289 cell NV3KNmM4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2fHN2lvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjd5IN88US=> NXfjWIJUW0GQR1XS
human CAL-12T cell NHzPUpBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVnJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1zMmSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlI{QSEQvF2= MlexV2FPT0WU
human T84 cell M1fHUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV\Rc5hKUW6qaXLpeIlwdiCxZjDoeY1idiCWOESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlI1KM7:TR?= Moj3V2FPT0WU
human KYSE-450 cell M4fKfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1\OeGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT20OVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ4NzFOwG0> M4WyVHNCVkeHUh?=
human LCLC-103H cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkewTY5pcWKrdHnvckBw\iCqdX3hckBNS0yFLUGwN2gh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjNzIH7N NEHtcpBUSU6JRWK=
human NCI-H810 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1TjNmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi4NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN{MjFOwG0> M1[1bHNCVkeHUh?=
human HUTU-80 cell NH\iflhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NU[2[WxNUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|Mkig{txO NVjqVmlVW0GQR1XS
human NCI-H460 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYLJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNE[wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{OzNizszN MorVV2FPT0WU
human HGC-27 cell MmrQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVHNWI9WUW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN|ODFOwG0> MV\TRW5ITVJ?
human HSC-2 cell NYTBb|NRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIrkcGFKdmirYnn0bY9vKG:oIHj1cYFvKEiVQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4{PjNibl2= NHz4PHRUSU6JRWK=
human IA-LM cell NY\wO5VYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV2zXG5zUW6qaXLpeIlwdiCxZjDoeY1idiCLQT3MUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzl|IN88US=> NHrWcopUSU6JRWK=
human HMV-II cell M1;LPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV3sbZpSUW6qaXLpeIlwdiCxZjDoeY1idiCKTW[tTWkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> NUC1ZlVCW0GQR1XS
human COLO-679 cell NIO0b49Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M12zXWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22O|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN7NDFOwG0> NIXseI5USU6JRWK=
human NCI-H2122 cell MoHvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV3ObpdXUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIyOjJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkSxJO69VQ>? MX\TRW5ITVJ?
human CAL-39 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnL2TY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQyOSEQvF2= MnTQV2FPT0WU
human SW954 cell MnjVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NILsOI5KdmirYnn0bY9vKG:oIHj1cYFvKFOZOUW0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE41OzZizszN M1iwXnNCVkeHUh?=
human LCLC-97TM1 cell MkDES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGXmeWhKdmirYnn0bY9vKG:oIHj1cYFvKEyFTFOtPVdVVTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS0OEDPxE1? MoKyV2FPT0WU
human KYSE-270 cell NIHLO4ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE41PDVizszN NGLscYFUSU6JRWK=
human NB13 cell NX;tSoVxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{nSemlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFQ4KM7:TR?= M4nMUnNCVkeHUh?=
human SK-LMS-1 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV\3XWNSUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MUXMuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNkS2JO69VQ>? NHziRnBUSU6JRWK=
human SW620 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{jzSGlvcGmkaYTpc44hd2ZiaIXtZY4hW1d4MkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlY1QCEQvF2= NHfMV2lUSU6JRWK=
human HCT-15 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYja[|BUUW6qaXLpeIlwdiCxZjDoeY1idiCKQ2StNVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjdzNzFOwG0> NF\1e5pUSU6JRWK=
human DU-145 cell NHnITHVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLnSpdPUW6qaXLpeIlwdiCxZjDoeY1idiCGVT2xOFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd{MTFOwG0> NETUdYdUSU6JRWK=
human ST486 cell NXLQcpRVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXjJcohq[mm2aX;uJI9nKGi3bXHuJHNVPDh4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD63NlEh|ryP MXHTRW5ITVJ?
human A427 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYjJcohq[mm2aX;uJI9nKGi3bXHuJGE1OjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkiyPEDPxE1? NXvsVnN{W0GQR1XS
human HT-1080 cell M4Lkfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mmf5TY5pcWKrdHnvckBw\iCqdX3hckBJXC1zMEiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PDJizszN Ml3wV2FPT0WU
human NCI-H2009 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVz4PGdzUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIxODliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki2NkDPxE1? M4XReHNCVkeHUh?=
human SK-LU-1 cell NFzuS2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NG\RWFNKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzVMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh4NjFOwG0> NF3PWWFUSU6JRWK=
human SCC-4 cell NEi2UoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4HCfWlvcGmkaYTpc44hd2ZiaIXtZY4hW0OFLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg4PyEQvF2= MmjaV2FPT0WU
human NCI-H1299 cell M3TjXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXL6T41lUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFEzQTliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki4JO69VQ>? MnH0V2FPT0WU
human NH-12 cell M3v6XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHfBN3VKdmirYnn0bY9vKG:oIHj1cYFvKE6KLUGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45QTFizszN Ml;vV2FPT0WU
human SK-UT-1 cell MljUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXHJcohq[mm2aX;uJI9nKGi3bXHuJHNMNVWWLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg6OiEQvF2= MV7TRW5ITVJ?
human Bel7402 MXTQdo9tcW[ncnH0bY9vKGG|c3H5 NEPyeo04OiCq NGL3VZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLlcFc1ODJiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmeiCJbH:gZZN{[XluIFnDOVA:OC57IN88US=> NHe4e4EzOjN{MEO1OC=>
human A388 cell M3HUcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUWwfVhRUW6qaXLpeIlwdiCxZjDoeY1idiCDM{i4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46PCEQvF2= NEfqOWtUSU6JRWK=
human SW982 cell NYjWVYt5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoLYTY5pcWKrdHnvckBw\iCqdX3hckBUXzl6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVk6KM7:TR?= NX6zW4k{W0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of RPMI 8226 cells, Epothilone B (2.5 mg/kg–4 mg/kg) prolongs survival and suppresses tumor growth. [4] Similarly, in mouse xenograft models of prostate cancer cells, including DU145 and PC3, Epothilone B at the same dose also inhibits tumor growth. [6]

Protocol

Kinase Assay:[1]
+ Expand

Tubulin polymerization assay:

Calf brain microtubule proteins (MTP) are purified, which includes approximately 15%–20% microtubule associated proteins. The buffer (MES buffer) used for the Epothilone B-microtubule studies contains 0.1 M 2-morpholinoethanesulfonic acid (MES), 1 mM EGTA, 0.5 mM MgCl2, and 3 M glycerol at pH 6.6. Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone B is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope and expressed as the mean with standard deviation obtained from three different concentrations.
Cell Research:[2]
+ Expand
  • Cell lines: KB3-1, KBV-1, Hela, and Hs578T cells
  • Concentrations: 0–1 μM
  • Incubation Time: 72 hours
  • Method: For mitotic block and aberrant mitosis, cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips. After 24 hours, cells are treated with Epothilone B and scored at regular intervals. For the cytotoxicity analysis, cells are counted and scored as trypan blue positive or negative. Concurrently, coverslips andaliquots of cells in the culture supernatant are fixed and stained with Hoechst33342 in PBS. These cells are scored for cells blocked at the G2/M transition and aberrant mitosis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Mice xenograft model of RPMI 8226 cells
  • Formulation: Dissolved in30% PEG-300
  • Dosages: 2.5 mg/kg–4 mg/kg
  • Administration: Inject intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.91 mM)
Ethanol 102 mg/mL (200.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.68
Formula

C27H41NO6S

CAS No. 152044-54-7
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00715013 Completed Recurrent Glioblastoma Planned for Reoperation University of Zurich July 2008 Phase 1|Phase 2
NCT00442741 Withdrawn Solid Tumors Novartis Pharmaceuticals|Novartis July 2007 Phase 1
NCT00496600 Completed Refractory Malignancy University of Medicine and Dentistry of New Jersey|Novartis Pharmaceuticals|National Cancer Institute (NCI)|Rutgers, The State University of New Jersey July 2007 Phase 1
NCT00468260 Terminated Advanced Malignancies Novartis Pharmaceuticals|Novartis May 2007 Phase 1
NCT00448396 Completed Advanced Malignancies Novartis Pharmaceuticals|Novartis March 2007 Phase 1
NCT00407251 Completed Hormone Refractory Prostate Cancer British Columbia Cancer Agency February 2007 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Microtubule Associated Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID