Home Cytoskeletal Signaling Microtubule Associated inhibitor Cucurbitacin B For research use only.

Cucurbitacin B

Cucurbitacin B, an oxygenated tetracyclic triterpenoid compound extracted from Cucurbitaceae plant species, is a long-term anticancer agent by disruption of microtubule polymerization.

Cucurbitacin B Chemical Structure

Cucurbitacin B Chemical Structure

CAS: 6199-67-3

Selleck's Cucurbitacin B has been cited by 3 publications

Purity & Quality Control

Batch: S816501 DMSO] 100 mg/mL] false] Ethanol] 50 mg/mL] false] Water] Insoluble] false Purity: 99.96%
99.96

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Biological Activity

Description Cucurbitacin B, an oxygenated tetracyclic triterpenoid compound extracted from Cucurbitaceae plant species, is a long-term anticancer agent by disruption of microtubule polymerization.
Targets
Microtubule [1]
In vitro
In vitro Cucurbitacin B inhibits the growth of 5 GBM cell lines: U87, U118, U343, U373 and T98G by affecting the cytoskeleton and the JNK pathway, with ED50 ranged from 5 nM to 100 nM. Cucurbitacin B also causes cell cycle arrest at the G2/M phase, decreases cell migration and induces apoptosis of GBM cells. [1] In human breast cancer cell lines, Cucurbitacin B also inhibits cell growth with ED50 varied from 30.3 nM to 418 nM, and rapidly induces cytoskeletal disruption. [2] In addition, Cucurbitacin B possesses immunomodulatory and anti-inflammatory effects, depending on the induction of heme oxygenase-1 expression via Nrf2 activation. [3]
Cell Research Cell lines U87, T98G, U118, U343, and U373 cells
Concentrations ~1 μM
Incubation Time 48 h
Method For proliferation measurements, the cells are placed into 96 well plates, and cell growth is measured at various times by MTT assay according to the protocol.
In Vivo
In vivo In nude mice bearing MDA-MB-231 tumors, Cucurbitacin B (1 mg/kg, i.p.) inhibits tumor growth by 55 %, and causes lower serum glucose levels. [2]

Chemical Information & Solubility

Molecular Weight 558.70 Formula

C32H46O8
CAS No. 6199-67-3 SDF Download Cucurbitacin B SDF
Smiles CC(=O)OC(C)(C)C=CC(=O)C(C)(C1C(CC2(C1(CC(=O)C3(C2CC=C4C3CC(C(=O)C4(C)C)O)C)C)C)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (178.98 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 50 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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