IWP-2

Catalog No.S7085

IWP-2 Chemical Structure

Molecular Weight(MW): 466.6

IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses.

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3 Customer Reviews

  • KIF3A knockdown promotes both ligand secretion and intracellular signalling of the Wnt/β-catenin pathway in NSCLC cell lines. (c,d) SuperTOPFlash reporter assay to examine the effect of KIF3A knockdown on signalling pathway activation by autocrine or exogenous WNTs in A549 and SW900 cell lines. A549 and SW900 cells were incubated with DMSO or IWP-2 (30 μ M) for 48 h, and then treated with BSA or WNT3A (100 ng/ml) for additional 24 h. Luciferase reporter activity was calculated by dividing TOP/RL ratio by FOP/RL ratio (n = 3; error bars indicate SEM; p-value was determined by one-way ANOVA). *P < 0.05; **P < 0.01.

    Sci Rep, 2016, 6:32770.. IWP-2 purchased from Selleck.

    (b-d) Combined and temporal treatment with IWP-2, SAG and FGF8 resulted in robust induction of MGE cells from H9 and H7 hESCs as well as iPSCs, assayed after 25 days of differentiation. The image in the insets is Hoechst-labeled nuclei of the same microscope field. Scale bar: 100 μm.

    Methods, 2016, 101:103-12.. IWP-2 purchased from Selleck.

  • H. IWP-2, an inhibitor of Wnt signaling, rescued the cell growth in A549 cells which were co-transfected with miR-520e and pcDNA-Zbtb7a.

    Biochem Biophys Res Commun, 2017, 486(1):49-56. IWP-2 purchased from Selleck.

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Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses.
Features Selective inhibitor of Porcn-mediated Wnt secretion.
Targets
Wnt [1]
(Cell-free assay)
27 nM
In vitro

IWP-2 is useful in both regenerative medicine and anticancer efforts. IWP-2 inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse L cells NVm2O3NSTnWwY4Tpc44h[XO|YYm= NIPYfHQzPCCq NVvYZ2xvUW6qaXLpeIlwdiCxZjDwc5JkfXCrbnWtcYVlcWG2ZXSgW451KHOrZ37hcIxqdmdiaX6gcY92e2ViTDDj[YxteyCjZoTldkAzPCCqcoOgZpkhW3C{aX7n[ZJKdWGpZYOtWI9x\myjc3igdoVxd3K2ZYKgZZN{[XluIFXDOVA:OzBibl2= NYfLZnFvOjN2N{ezOlU>

... Click to View More Cell Line Experimental Data

Protocol

Cell Research:

[1]

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  • Cell lines: L-Wnt-STF cells
  • Concentrations: 5 μM
  • Incubation Time: 24 h
  • Method:

    L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Crl:CD1 (ICR) and FVB/N female mice
  • Formulation: encapsulated in liposomes
  • Dosages: 50 µg
  • Administration: s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMF 22 mg/mL warmed (47.14 mM)
DMSO 4 mg/mL warmed (8.57 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 466.6
Formula

C22H18N4O2S3

CAS No. 686770-61-6
Storage powder
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID