Methyl Vanillate

Catalog No.S3630

Methyl Vanillate Chemical Structure

Molecular Weight(MW): 182.17

Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.

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Biological Activity

Description Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.
Targets
Wnt/β-catenin [1]
()
In vitro

Methyl vanillate (MV) activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro. It increases the expression of differentiation markers RUNX2, BMP2, ALP, and OCN in a dose-dependent manner. MV decreases and increases the expression of RANKL and OPG, respectively. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts[1].

In vivo Methyl vanillate rescues trabecular or cortical femoral bone loss in the ovariectomized mice without inducing any significant weight changes or abnormality in liver tissue when administrated orally[1].

Protocol

Cell Research:[1]
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  • Cell lines: Calvarial osteoblasts
  • Concentrations: 10 and 20 μM
  • Incubation Time: 24 h
  • Method: Calvarial osteoblasts are seeded onto a coverslip in 12-well plates at a density of 3×104 cells/well. After 24 h, HDT extract or MV is added for another 24 h and the cells are subsequently fixed with 4% PFA for 10 min. Cells are permeabilized with 0.2% Triton X-100 for 15 min and incubated for 30 min with 5% bovine serum albumin (BSA) blocking solution. The cells are incubated with the primary antibody (β-catenin) overnight at 4°C and then washed with PBS three times. Cells are then incubated with Alexa Fluor 502-conjugated IgG secondary antibody is incubated for 1 h, followed by incubation with DAPI for 5 min. The cells are mounted in Gel/Mount media. The fluorescence signal is captured using confocal microscopy.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: ICR mice
  • Formulation: corn oil
  • Dosages: 25 and 100 mg/kg
  • Administration: oral
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 36 mg/mL (197.61 mM)
Ethanol 36 mg/mL (197.61 mM)
Water 12 mg/mL (65.87 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 182.17
Formula

C9H10O4

CAS No. 3943-74-6
Storage powder
in solvent
Synonyms N/A

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Wnt/beta-catenin Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID