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Cisplatin

Cisplatin is an inorganic and water-soluble platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts.

Catalog No.S1166

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50mg

USD 97

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Cisplatin Chemical Structure
Molecular Weight: 300.05

Validation & Quality Control

Product Citations(1)

PLoS One, 2013, 8(1), e54595

Customer Reviews(1) in vivo invitation

Quality Control & MSDS

Related Compound Libraries

Cisplatin is available in the following compound libraries:

FDA Approved Drug Library (96-well) Chemotherapeutic Agent Library (96-well) Chemical Library (96-well) Cambridge Cancer Compound Library(96-well)

Product Information

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Research Area
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Dr. Antonino Maria Sparta demonstrates a breakthrough in leukemia research by utilizing two inhibitors produced by Selleck Chemicals.

Cat.No.	Product Name	Solubility (25°C)
S1166	Cisplatin	<1 mg/mL	60 mg/mL	<1 mg/mL
S1215	Carboplatin	<1 mg/mL	<1 mg/mL	<1 mg/mL
S1149	Gemcitabine HCl (Gemzar)	23 mg/mL	<1 mg/mL	<1 mg/mL
S1491	Fludarabine (Fludara)	<1 mg/mL	57 mg/mL	<1 mg/mL
S1135	Pemetrexed (Alimta)	94 mg/mL	1 mg/mL	<1 mg/mL
S1224	Oxaliplatin (Eloxatin)	<1 mg/mL	14 mg/mL	<1 mg/mL
S1229	Fludarabine Phosphate (Fludara)	6 mg/mL	73 mg/mL	<1 mg/mL
S1214	Bleomycin sulfate	154 mg/mL	153 mg/mL	<1 mg/mL
S2684	CX-5461	<1 mg/mL	0.02 mg/mL	<1 mg/mL
S1209	Adrucil(Fluorouracil)	<1 mg/mL	26 mg/mL	<1 mg/mL
S1156	Capecitabine (Xeloda)	6 mg/mL	72 mg/mL	72 mg/mL
S1714	Gemcitabine(Gemzar)	<1 mg/mL	15 mg/mL	<1 mg/mL
S1648	Cytarabine	48 mg/mL	1 mg/mL	<1 mg/mL
S1213	Nelarabine (Arranon)	3 mg/mL	60 mg/mL	<1 mg/mL
S1217	Clafen (Cyclophosphamide)	43 mg/mL	52 mg/mL	52 mg/mL
S1302	Ifosfamide	52 mg/mL	52 mg/mL	52 mg/mL
S1221	Dacarbazine (DTIC-Dome)	<1 mg/mL	3 mg/mL	<1 mg/mL
S1334	Flupirtine maleate	<1 mg/mL	84 mg/mL	3 mg/mL
S1384	Mizoribine (Bredinin)	52 mg/mL	1 mg/mL	<1 mg/mL
S2678	NSC-207895 (XI-006)	<1 mg/mL	0.4 mg/mL	<1 mg/mL
S1199	Cladribine	<1 mg/mL	57 mg/mL	<1 mg/mL
S1218	Clofarabine	<1 mg/mL	61 mg/mL	<1 mg/mL
S1289	Carmofur	<1 mg/mL	52 mg/mL	10 mg/mL
S1299	Floxuridine (Fludara)	49 mg/mL	49 mg/mL	10 mg/mL
S1305	Mercaptopurine	<1 mg/mL	30 mg/mL	<1 mg/mL
S1192	Raltitrexed (Tomudex)	<1 mg/mL	92 mg/mL	<1 mg/mL
S1300	Ftorafur	8 mg/mL	40 mg/mL	7 mg/mL
S2554	Daphnetin	<1 mg/mL	36 mg/mL	<1 mg/mL
S1981	Adenine	<1 mg/mL	<1 mg/mL	<1 mg/mL
S1983	Adenine HCl	4 mg/mL	8 mg/mL	<1 mg/mL
S1982	Adenine sulfate	2 mg/mL	<1 mg/mL	<1 mg/mL
S1995	Procarbazine HCl (Matulane)	52 mg/mL	<1 mg/mL	52 mg/mL
S2029	Uridine	49 mg/mL	49 mg/mL	<1 mg/mL

Product Description

Biological Activity Protocol Chemical Information Customer Reviews Product Citations Tech Support & FAQs

Biological Activity

Description	Cisplatin is an inorganic and water-soluble platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts.
Targets
IC50
In vitro	Cisplatin induces cytotoxic by interaction with DNA to form DNA adducts which activate several signal transduction pathways, including Erk, p53, p73, and MAPK, which culminates in the activation of apoptosis. ^[1] Cisplatin (30 mM) treated for 6 h induces an apparent activation of Erk in HeLa cells, which is sustained over the following 14 h period. Cisplatin also shows an effective antineoplastic activity by inducing tumor cells death. Cisplatin displays ability to cause renal proximal tubular cell (RPTC) apoptosis, causing cell shrinkage, a 50-fold increase in caspase 3 activity, a 4-fold increase in phosphatidylserine externalization, and 5- and 15-fold increases in chromatin condensation and DNA hypoploidy, respectively. ^[4] Cisplatin (800 μM) causes typical features of necrosis of RPTC after treatment for 4 hr. ^[5]
In vivo	Cisplatin has been demonstrated to be efficient in regression tumor growth in a wide variety of animal tumors models, including head and neck cancer xenografts, cervical squamous carcinoma xenografts, testicular carcinoma xenografts, ovarian cancer xenografts, breast carcinoma xenografts, colonic carcinoma, heterotransplanted hepatoblastoma, and so on. Cisplatin (5 mg/kg) given weekly i.v. at the day 1 and 7 induces a tumor growth inhibition (GI) of 77.5% and 85.1% of the serous xenografts Ov.Ri(C) and OVCAR-3, respectively. ^[6]
Clinical Trials	A phase III trial of tri-weekly Cisplatin based chemoradiation in locally advanced cervical cancer (TACO) is currently in recruiting.
Features	Cisplatin is one of the most widely used and most potent chemotherapy agents.

Protocol(Only for Reference)

Cell Assay: ^[3]

Cell lines	Leukemia L1210/0 cells
Concentrations	7 μg/mL
Incubation Time	2 hours
Method	L1210/0 cells are maintained in an exponential suspension culture at 37 ℃ in a humidified atmosphere of 5% CO₂ in McCoy's medium 5a (modified), supplemented with 15% calfserum, and Fungizone. L1210/0 cells are incubated in Cisplatin (7 μg/mL) for 2 hr at 37 ℃. To measure growth inhibition, the cells are centrifuged, washed once, resuspended in fresh medium at 30 × 10³ to 50 × 10³ cells/mL, and incubated for 3 days. Cell numbers are determined on a Coulter Counter. An aliquot of cells is diluted with an equal volume of 0.4% trypan blue. Viability is recorded as the percentage of cells that has excluded trypan blue. Cells incubated with Cisplatin as above are also diluted into 0.1% agar and allowed to grow for 2 weeks when colonies are counted.

Animal Study: ^[6]

Animal Models	The human ovarian cancer xenografts OVCAR-3 in nude mice
Formulation	0.9% NaCl solution
Dosages	5 mg/kg
Administration	Given i.v. at day 0 (when tumors had reached a volume between 50 and 150 mm³) and day 7.

References

Chemical Information

Download Cisplatin SDF

Molecular Weight (MW)	300.05
Formula	Cl₂H₆N₂Pt
CAS No.	15663-27-1
Synonyms	cisplatinum, CDDP, Platinol and Platinol-AQ

Solubility (25°C) DMSO 60 mg/mL Water <1 mg/mL Ethanol <1 mg/mL
Storage	2 years -20°CPowder
	2 weeks4°Cin DMSO
	6 months-80°Cin DMSO

Chemical Name	Platinum, diamminedichloro-, (SP-4-2)-

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Research Area

Customer Reviews (1)

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Rating

Source

Dr. Edita Aksamitiene from Thomas Jefferson University. Cisplatin purchased from Selleck

Method

Cell viability assay

Cell Lines

pancreatic cancer cells

Concentrations

0-1000 nM

Incubation Time

72 h

Results

Cisplatin potently inhibited the survival of Capan-2 cells in a dose-dependent manner.

Product Citations (1)

Suppression of AKT Anti-Apoptotic Signaling by a Novel Drug Candidate Results in Growth Arrest and Apoptosis of Hepatocellular Carcinoma Cells. [Cuconati A, et al. PLoS One 2013;8(1), e54595]
PubMed: 23355882

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cisplatin