6-Thio-dG | 99.86%(HPLC) | In Stock | DNA/RNA Synthesis inhibitor

6-thio-dG (β-TGdR) is a nucleoside analog and telomerase substrate.

6-Thio-dG Chemical Structure

CAS: 789-61-7

Selleck's 6-Thio-dG has been cited by 2 publications

Purity & Quality Control

Batch: Purity: 99.86%

99.86

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Signaling Pathway

DNA/RNA Synthesis Signaling Pathway

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Biological Activity

Description	6-thio-dG (β-TGdR) is a nucleoside analog and telomerase substrate.

In vitro
In vitro	6-Thio-dG induces telomere dysfunction and results in progressive telomere shortening in telomerase-positive human cancer cells and hTERT-expressing human fibroblasts. In tested cancer cells, 6-thio-dG inhibits cell viability with IC50 values between 0.7 and 2.9 μM. ^[1]
Cell Research	Cell lines	HCT11, A549, H2882, HCC2429, HCC827, HCC15, H2087, HCC4017, HCC515, H2009, BJ and HCEC1 cells
	Concentrations	~10 μM
	Incubation Time	7 d
	Method	Cells are plated in growth media in 96-well plates. Cells are incubated for 1 week and treated with varying concentrations of 6-thio-dG and 6-thioguanine or DMSO every 3 days. The 96-well plates are analyzed according the manufacturer’s directions for the CellTiterGlo luminescent cell viability assay to obtain IC50 values. The IC50 is defined as the concentration of drug at which 50% of the cells are inhibited by the drug. Sigmoidal dose–response curves are used to calculate IC50 values. All samples are analyzed in triplicate, and SDs are from two independent experiments.

In Vivo
In vivo	In mice bearing A549 lung cancer xenografts, 6-Thio-dG (2 mg/kg, i.p.) decreases the tumorigenicity of A549 Cells by inducing telomere dysfunction. ^[1]
Animal Research	Animal Models	Mice bearing A549 lung cancer xenografts
	Dosages	~2 mg/kg
	Administration	i.p.

References
[1] Mender I, et al. Cancer Discov. 2015, 5(1), 82-95.

References

[1] Mender I, et al. Cancer Discov. 2015, 5(1), 82-95.

Chemical Information & Solubility

Molecular Weight	283.31	Formula	C₁₀H₁₃N₅O₃S
CAS No.	789-61-7	SDF	Download 6-Thio-dG SDF
Smiles	C1C(C(OC1N2C=NC3=C2NC(=NC3=S)N)CO)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 56 mg/mL ( (197.66 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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