Home DNA Damage/DNA Repair DNA/RNA Synthesis chemical Thymidine For research use only.

Thymidine

Synonyms: NSC 21548, Deoxythymidine, 2'-Deoxythymidine, 5-Methyldeoxyuridine, DThyd

Thymidine is a pyrimidine nucleoside that is composed of the pyrimidine base thymine attached to the sugar deoxyribose. As a constituent of DNA, thymidine pairs with adenine in the DNA double helix.

Thymidine Chemical Structure

Thymidine Chemical Structure

CAS: 50-89-5

Selleck's Thymidine has been cited by 6 publications

Purity & Quality Control

Batch: Purity: 99.98%
99.98

Thymidine Related Products

Signaling Pathway

DNA/RNA Synthesis Signaling Pathways

DNA/RNA Synthesis Signaling Pathway

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Vero Antiviral assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay, IC50 = 0.0003 μM. 10464017
HepG2.2.15 Antiviral assay Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR, EC50 = 0.3 μM. 17404006
HL-60 Function assay The compound was tested for [3H]TdR incorporation into DNA in HL-60 cells, ID50 = 0.61 μM. 2840503
Vero Function assay Compound was evaluated for Kinetic constant for cellular thymidine kinase in Vero cells, Km = 0.81 μM. 3033244
HEL Antiviral assay 3 days Antiviral activity against Human adenovirus 2 infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by microscopic method, EC50 = 22 μM. 30286952
ONS Function assay 10 uM 24 hrs Induction of phenotypic perturbation in human ONS cells assessed as decrease in EEA1-associated early endosomes level in at 10 uM after 24 hrs by EEA1 staining based assay relative to control 26883470
ONS Function assay 10 uM 24 hrs Induction of phenotypic perturbation in human ONS cells assessed as decrease in autophagy markers level in at 10 uM after 24 hrs relative to control 26883470
Click to View More Cell Line Experimental Data

Biological Activity

Description Thymidine is a pyrimidine nucleoside that is composed of the pyrimidine base thymine attached to the sugar deoxyribose. As a constituent of DNA, thymidine pairs with adenine in the DNA double helix.
In vitro
In vitro

Thymidine (NSC 21548), a pyrimidine nucleoside that is composed of the pyrimidine base thymine attached to the sugar deoxyribose, pairs with adenine when DNA synthesis.[1]
Cell Research Cell lines USP28-depleted MDA-MB-231 cells
Concentrations 4 mM
Incubation Time 18 h
Method

72 hours after shRNA lentiviruses infection, MDA-MB-231 cells are treated with 4 mM Thymidine for 18 hours. Release and harvest cells at the time points of 0, 6 and 18 hours, and then determine the cell cycle distribution by FACS analysis.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting
Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer
Gynecologic Oncology Group|National Cancer Institute (NCI)|GOG Foundation
 January 2100 --
NCT04598516 Withdrawn
Acute Kidney Injury (Nontraumatic)|Contrast-induced Nephropathy|Renal Insufficiency
Maastricht University Medical Center
 November 1 2097 --
NCT04662164 Withdrawn
Type 2 Diabetes Patients
Shanghai HEP Pharmaceutical Co. Ltd.
 December 2025 Phase 1|Phase 2
NCT01037387 Recruiting
Chronic Obstructive Pulmonary Disease|Hypercapnic
Hospital Universitario La Paz
 December 2025 Phase 4

Chemical Information & Solubility

Molecular Weight 242.23 Formula

C10H14N2O5
CAS No. 50-89-5 SDF Download Thymidine SDF
Smiles CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 48 mg/mL ( (198.15 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 48 mg/mL

Ethanol : 9 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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