Home MAPK S6 Kinase inhibitor - p38 MAPK inhibitor - ERK activator - JNK activator 3'-Hydroxypterostilbene For research use only.

3'-Hydroxypterostilbene

Synonyms: 3'-HPT

3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. 3'-Hydroxypterostilbene, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.

3'-Hydroxypterostilbene Chemical Structure

3'-Hydroxypterostilbene Chemical Structure

CAS: 475231-21-1

Purity & Quality Control

Batch: S394001 DMSO] 54 mg/mL] false] Ethanol] 54 mg/mL] false] Water] Insoluble] false Purity: 99.55%
99.55

3'-Hydroxypterostilbene Related Products

Signaling Pathway

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Biological Activity

Description 3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. 3'-Hydroxypterostilbene, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.
Targets
p70S6K [1] p38MAPK [1]
In vitro
In vitro 3'-Hydroxypterostilbene decreases cell growth in cultured human colon cancer cells (COLO 205, HCT-116, and HT-29) in a dose-dependent manner, with IC50 values of 9.0, 40.2, and 70.9 µM, respectively. 3'-Hydroxypterostilbene effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy. Treatment with HPSB causes reduction of mitochondrial membrane potential and induction of caspase-3 and caspage-9, which is associated with the degradation of DFF-45 and PARP, precedes the onset of apoptosis. It significantly down-regulates phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) signalings including decreased the phosphorylation of mammalian target of rapamycin (mTOR)[1].
Cell Research Cell lines COLO 205 cells
Concentrations 5-100 µM
Incubation Time 24 h
Method

For sub-G1 cell population analysis, COLO 205 cells (2×105 cells/mL) are cultured in 12 well and treatment with various concentrations of pterostilbene or 3′-hydroxypterostilbene for 24 h. The cells are then harvested, washed with PBS, resuspended in 200 µL of PBS, and fixed in 800 µL of iced 100% ethanol at -20°C. After being left to stand overnight, the cell pellets are collected by centrifugation, resuspended in 1 mL of hypotonic buffer (0.5% Triton X-100 in PBS and 0.5 µg/mL RNase) with PI (50 µg/mL) and incubated at 37°C in the dark for 30 min. Fluorescence emitted from the PI-DNA complex is quantitated after excitation of the fluorescent dye.
In Vivo
In vivo 3'-Hydroxypterostilbene by i.p. injection has anti-tumor efficacy gainst colon cancer through the inhibition of inflammation, metastasis, and angiogenesis as well as through the induction of apoptosis[1].
Animal Research Animal Models nude mice bearing COLO 205 tumor xenografts
Dosages 10 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting
Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer
Gynecologic Oncology Group|National Cancer Institute (NCI)|GOG Foundation
 January 2100 --
NCT05118594 Not yet recruiting
Major Depressive Disorder|Dysthymic Disorder|Specific Phobia|Social Anxiety Disorder|Panic Disorder|Agoraphobia|Generalized Anxiety Disorder
National Council of Scientific and Technical Research Argentina
 February 2026 Not Applicable

Chemical Information & Solubility

Molecular Weight 272.30 Formula

C16H16O4
CAS No. 475231-21-1 SDF Download 3'-Hydroxypterostilbene SDF
Smiles COC1=CC(=CC(=C1)C=CC2=CC(=C(C=C2)O)O)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 54 mg/mL ( (198.31 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 54 mg/mL

Water : Insoluble


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In vivo
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