Name: Spermidine
Brand: Selleck Chemicals
SKU: S3569
Availability: InStock
Rating: 5 (7 reviews)

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

97

Related Targets	CXCR Nrf2 Mitophagy LRRK2 ULK FKBP Heme Oxygenase cGAS LC3 Cell wall
Other Autophagy Inhibitors	Resveratrol (trans-Resveratrol) Spautin-1 PIK-III DC661 Lys05 Trihydrochloride Autophinib SMER28 EN6 Flubendazole QX77

Solubility

In vitro
Batch:

Water : 100 mg/mL

DMSO : 20 mg/mL (137.69 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (6.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (6.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	145.25	Formula	C₇H₁₉N₃	Storage (From the date of receipt)	2 years -20°C liquid
CAS No.	124-20-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Mechanism of Action

In vitro	Spermidine and spermine significantly increases the viability and proliferation of SKOV-3 cells in a time-dependent manner, and decreases the apoptotic effect of Difluoromethylornithine (DFMO) in SKOV-3 cells.
In vivo	Spermidine, a selective activator of TC45, rescues exacerbated phenotype of Hematopoietic stem and progenitor cells (HSPCs) in IFN‐γ‐treated Hemgn_-/- mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/34638596/ [3] https://pubmed.ncbi.nlm.nih.gov/30306826/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06017219	Active not recruiting	Healthy Subjects	Chrysea Labs Lda	May 5 2023	Not Applicable
NCT05421546	Completed	COVID-19\|Vaccine Reaction	University of Oxford	August 1 2022	Not Applicable
NCT04138134	Active not recruiting	Venous Disease	Neuromed IRCCS\|Principal investigator: Giacomo Frati\|Co-investigator: Francesco Pompeo	February 1 2022	--
NCT04823806	Active not recruiting	Depression\|Healthy	University Hospital Bonn	December 1 2020	Not Applicable
NCT02855918	Completed	Major Depression	University Hospital Montpellier\|Institut National de la Santé Et de la Recherche Médicale France\|Sys2Diag Mixt laboratory CNRS/Alcediag Montpellier	September 23 2016	Not Applicable

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Spermidine Autophagy activator

Chemical Structure

Molecular Weight: 145.25