EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride)

EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride) acts as an inhibitor of macropinocytosis and sodium-hydrogen exchangers(NHE) (IC50=0.033μg/mL). EIPA blocks the activity of Na(+)/H(+) exchanger, which are plasma membrane proteins implicated in all forms of macropinocytosis.

EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride) Chemical Structure

EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride) Chemical Structure

CAS: 1154-25-2

Selleck's EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride) has been cited by 3 publications

Purity & Quality Control

Batch: Purity: 99.77%
99.77

EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride) Related Products

Choose Selective Sodium Channel Inhibitors

Biological Activity

Description EIPA (5-(N-Ethyl-N-isopropyl)-Amiloride) acts as an inhibitor of macropinocytosis and sodium-hydrogen exchangers(NHE) (IC50=0.033μg/mL). EIPA blocks the activity of Na(+)/H(+) exchanger, which are plasma membrane proteins implicated in all forms of macropinocytosis.
Targets
NHE [1]
(in the Sodium-hydrogen exchanger activity assay)
0.033 μg/mL
In vitro
In vitro

5-(N-Ethyl-N-isopropyl)-Amiloride (EIPA) rapidly and reversibly block Ca2+-activated TRPP3 channel activation, with IC50s of 10.5 μM.[2]

Cell Research Cell lines Xenopus laevis oocyte
Concentrations 100 μM
Incubation Time 30 min
Method

The uptake of radioactive 45Ca2+ mediated by TRPP3 channel  is tested using the standard NaCl-containing solution, plus 1 mM nonradiolabeled CaCl2 and radiolabeled 45Ca2+ in the presence and absence of 100 μM EIPA. Control uptake level is obtained using H2O-injected oocytes.

In Vivo
In vivo

5-(N-Ethyl-N-isopropyl)-Amiloride (EIPA) downregulates endothelial cell activation of NF-kappaB and VCAM-1 expression and attenuates the early inflammatory stages of atherogenesis and stent intimal formation[3].

Animal Research Animal Models Murine Model of Atherogenesis (apoE-/- mice)
Dosages 3 mg/kg
Administration s.c.

Chemical Information & Solubility

Molecular Weight 299.76 Formula

C11H18ClN7O

CAS No. 1154-25-2 SDF --
Smiles CCN(C(C)C)C1=C(Cl)N=C(C(=N1)N)C(=O)NC(N)=N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 60 mg/mL ( (200.16 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 30 mg/mL

Water : 2 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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