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PMA (Phorbol 12-myristate 13-acetate)

Name: PMA (Phorbol 12-myristate 13-acetate)
Brand: Selleck Chemicals
SKU: S7791
Rating: 5 (46 reviews)

Synonyms: 12-O-Tetradecanoylphorbol-13-acetate,TPA, Phorbol myristate acetate

Catalog No.S7791 Purity: 99.93%

PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).PMA has strong excitability on the skin and mucous membrane. Special protection is required when using PMA. Wear gloves and mask to avoid direct contact in any way.

PMA (Phorbol 12-myristate 13-acetate) Chemical Structure

CAS: 16561-29-8

Selleck's PMA (Phorbol 12-myristate 13-acetate) has been cited by 46 publications

Purity & Quality Control

Batch: Purity: 99.93%

99.93

Products often used together with PMA (Phorbol 12-myristate 13-acetate)

Tangeretin

Tangeretin significantly inhibits Phorbol 12-myristate 13-acetate (PMA) stimulated epidermal hyperplasia and intra-epidermal neutrophilic abscesses in mice.

Chang SN, et al. Int J Mol Sci. 2020 Dec 4;21(23):9261.

Retinoic acid (Tretinoin)

Phorbol 12-myristate 13-acetate and ALL-Trans Retinoic acid combination mediates the differentiation of HL60 and NB4 cell lines and changes their drug sensitivity.

Jasek E, et al. Folia Histochem Cytobiol. 2008;46(3):323-30.

Curcumin

Curcumin reduces the Phorbol 12-myristate 13-acetate (PMA)-induced elevation of uric acid in NIH3T3 cells.

Lin JK, et al. Carcinogenesis. 1994 Aug;15(8):1717-21.

Forskolin (Colforsin)

Phorbol 12-myristate 13-acetate (PMA) and Forskolin are strongly synergistic in altering endothelial cell morphology.

Antonov AS, et al. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9704-8.

PMA (Phorbol 12-myristate 13-acetate) Related Products

Related Targets	PKCα PKCβ PKCγ PKCδ PKCε PKCζ PKCη PKCθ PKCι	Click to Expand
Related Compound Libraries	Kinase Inhibitor Library Angiogenesis Related compound Library TGF-beta/Smad compound library Cytoskeletal Signaling Pathway Compound Library Anti-Cardiovascular Disease Compound Library	Click to Expand

Signaling Pathway

PKC Signaling Pathway

Choose Selective PKC Inhibitors

Biological Activity

Description	PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).PMA has strong excitability on the skin and mucous membrane. Special protection is required when using PMA. Wear gloves and mask to avoid direct contact in any way.

In vitro
In vitro	In endothelial cells, PMA increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.^[1]
Cell Research	Cell lines	Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs)
	Concentrations	20 ng/mL
	Incubation Time	14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein
	Method	After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	pERK / ERK1 / pp38 / p38 p-GSK3β / GSK3β / Lamin B2		22977714
	Immunofluorescence	cTnT / β-MHC / CD90		27405982

In Vivo
In vivo	In the zebrafish model, PMA initially enhances endothelial cell migration, subsequently activates the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.^[1]
Animal Research	Animal Models	Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio)
	Dosages	20 ng/mL
	Administration	--

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05908227	Recruiting	Respiratory Distress Syndrome	Insel Gruppe AG University Hospital Bern	July 1 2023	Not Applicable
NCT06087666	Recruiting	Preterm	Instituto de Investigación Hospital Universitario La Paz	February 1 2023	Not Applicable
NCT05446272	Recruiting	Extubation Failure\|Bronchopulmonary Dysplasia\|Death	University of Pennsylvania\|National Heart Lung and Blood Institute (NHLBI)	August 3 2022	Not Applicable

References

[1] Wen HC, et al. Sci Rep. 2018 Nov 2;8(1):16247.

Chemical Information & Solubility

Molecular Weight	616.83	Formula	C₃₆H₅₆O₈
CAS No.	16561-29-8	SDF	--
Smiles	CCCCCCCCCCCCCC(=O)OC1C(C2(C(C=C(CC3(C2C=C(C3=O)C)O)CO)C4C1(C4(C)C)OC(=O)C)O)C
Storage (From the date of receipt)	3 years -20°C(in the dark) powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C(in the dark) powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (162.11 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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