Oleuropein

Catalog No.S7867

For research use only.

Oleuropein is an antioxidant polyphenol isolated from olive leaf. Oleuropein exerts antioxidant, anti-inflammatory and anti-atherogenic effects and suppresses the adipocyte differentiation in vitro. Oleuropein inhibits PPARγ activity. Oleuropein exerts anti-adipogenic effect through direct inhibition of PPARγ transcriptional activity. Oleuropein also inhibits aromatase. Oleuropein induces apoptosis. Phase 2.

Oleuropein Chemical Structure

CAS No. 32619-42-4

Selleck's Oleuropein has been cited by 1 Publication

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Biological Activity

Description Oleuropein is an antioxidant polyphenol isolated from olive leaf. Oleuropein exerts antioxidant, anti-inflammatory and anti-atherogenic effects and suppresses the adipocyte differentiation in vitro. Oleuropein inhibits PPARγ activity. Oleuropein exerts anti-adipogenic effect through direct inhibition of PPARγ transcriptional activity. Oleuropein also inhibits aromatase. Oleuropein induces apoptosis. Phase 2.
In vitro

In both J774A.1 cells and primary mouse macrophages, Oleuropein increases LPS-induced NO production. [1] Oleuropein prevents oxidative myocardial injury induced by ischemia and reperfusion. [2] In TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells, Oleuropein inhibits cell proliferation and migration. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat IEC-18 cells MVjGeY5kfGmxbjDhd5NigQ>? NVTJS25vUW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBxOzhiTVHQT{BxcG:|cHjvdplt[XSrb36gbY4hemG2IFnFR{0yQCClZXzsd{BifCBzMECgeW0hcW6ldXLheIVlKG[xcjCxJIhzKHC{aX;yJJRwKEySUzDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiM{CgcYlveyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NHjDOpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e1PFEyOCd-MkO3OVgyOTB:L3G+
In vivo In swiss albino mice that spontaneously develop soft tissue sarcomas, Oleuropein (i.p. or p.o.) completely regresses tumors. [3] In rats, Oleuropein (40 mg/kg, p.o.) protects from cardiac remodeling process after isoproterenol-induced myocardial infarction through inhibiting angiotensin-converting enzyme activity. [4]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells
  • Concentrations: --
  • Incubation Time: 5 days
  • Method:

    Approximately 5 × 103 cells (TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib) are seeded in triplicate wells of 96-well plates in 100 μl of their respective growth medium with increasing doses of purified Oleuropein. Cell proliferation is determined on day 5 using the CellTiter MTS cell proliferation kit according to the manufacturer’s instructions. Absorbance at 490 nm is measured with a Bio-Tek Instruments microplate reader. Background readings are subtracted from the experimental readings.

Animal Research:

[3]

  • Animal Models: Swiss albino mice that spontaneously develops soft tissue sarcomas
  • Dosages: 50 μg/mouse
  • Administration: i.p. or p.o.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
PBS
For best results, use promptly after mixing.

30mg/mL

Chemical Information

Molecular Weight 540.51
Formula

C25H32O13

CAS No. 32619-42-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC=C1C(C(=COC1OC2C(C(C(C(O2)CO)O)O)O)C(=O)OC)CC(=O)OCCC3=CC(=C(C=C3)O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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