Oleuropein

Oleuropein is an antioxidant polyphenol isolated from olive leaf. Oleuropein exerts antioxidant, anti-inflammatory and anti-atherogenic effects and suppresses the adipocyte differentiation in vitro. Oleuropein inhibits PPARγ activity. Oleuropein exerts anti-adipogenic effect through direct inhibition of PPARγ transcriptional activity. Oleuropein also inhibits aromatase. Oleuropein induces apoptosis. Phase 2.

Oleuropein Chemical Structure

Oleuropein Chemical Structure

CAS: 32619-42-4

Selleck's Oleuropein has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.73%
99.73

Oleuropein Related Products

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat IEC-18 cells Function assay Inhibition of LPS-induced p38 MAPK phosphorylation in rat IEC-18 cells at 100 uM incubated for 1 hr prior to LPS challenge measured after 30 mins by Western blot analysis 23758110
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Biological Activity

Description Oleuropein is an antioxidant polyphenol isolated from olive leaf. Oleuropein exerts antioxidant, anti-inflammatory and anti-atherogenic effects and suppresses the adipocyte differentiation in vitro. Oleuropein inhibits PPARγ activity. Oleuropein exerts anti-adipogenic effect through direct inhibition of PPARγ transcriptional activity. Oleuropein also inhibits aromatase. Oleuropein induces apoptosis. Phase 2.
In vitro
In vitro In both J774A.1 cells and primary mouse macrophages, Oleuropein increases LPS-induced NO production. [1] Oleuropein prevents oxidative myocardial injury induced by ischemia and reperfusion. [2] In TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells, Oleuropein inhibits cell proliferation and migration. [3]
Cell Research Cell lines TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib cells
Concentrations --
Incubation Time 5 days
Method

Approximately 5 × 103 cells (TF-1a; 786-O, T-47D, RPMI-7951, LoVo, and NL-Fib) are seeded in triplicate wells of 96-well plates in 100 μl of their respective growth medium with increasing doses of purified Oleuropein. Cell proliferation is determined on day 5 using the CellTiter MTS cell proliferation kit according to the manufacturer’s instructions. Absorbance at 490 nm is measured with a Bio-Tek Instruments microplate reader. Background readings are subtracted from the experimental readings.

In Vivo
In vivo In swiss albino mice that spontaneously develop soft tissue sarcomas, Oleuropein (i.p. or p.o.) completely regresses tumors. [3] In rats, Oleuropein (40 mg/kg, p.o.) protects from cardiac remodeling process after isoproterenol-induced myocardial infarction through inhibiting angiotensin-converting enzyme activity. [4]
Animal Research Animal Models Swiss albino mice that spontaneously develops soft tissue sarcomas
Dosages 50 μg/mouse
Administration i.p. or p.o.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05217433 Completed
Muscular Fatigue
Société des Produits Nestlé (SPN)|Maastricht University Medical Center
November 4 2021 Not Applicable

Chemical Information & Solubility

Molecular Weight 540.51 Formula

C25H32O13

CAS No. 32619-42-4 SDF Download Oleuropein SDF
Smiles CC=C1C(C(=COC1OC2C(C(C(C(O2)CO)O)O)O)C(=O)OC)CC(=O)OCCC3=CC(=C(C=C3)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (185.01 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : 50 mg/mL


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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