(+)-Catechin

Catalog No.S4722 Synonyms: Cianidanol, Catechinic acid, Catechuic acid

For research use only.

(+)-Catechin (Cianidanol, Catechinic acid, Catechuic acid) is an antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.

(+)-Catechin Chemical Structure

CAS No. 154-23-4

Selleck's (+)-Catechin has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description (+)-Catechin (Cianidanol, Catechinic acid, Catechuic acid) is an antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.
Targets
COX-1 [3]
(Cell-based assay)
COX-2 [3]
(Cell-based assay)
80 μM 130 μM
In vitro

The catechin flavonoid reduces proliferation and induces apoptosis of murine lymphoma cells LB02 through modulation of antiapoptotic proteins[1]. Treatment of human prostate cancer cells with catechin induces apoptosis and results in effects on a variety of survival signals suggesting the potential of these compounds as chemopreventive agents for prostate cancer[2]. Catechin is well known to inhibit cyclooxygenase activity with reported IC50 values ranging from 4O μM to 943 μM. It has also been reported to act as an inhibitor of COX-1 and COX-2 with IC50 of about 80 µM and 130 μM[3].

In vivo Dietary catechin significantly delayed tumor onset[5]. Continued intake of catechin, a potential antioxidant, prevents memory regression and DNA oxidative damage in senescence-accelerated (SAMP10) mice. It does not prolong the lifetime of SAMP10 mice, but it does delay brain senescence[6]. (+)-Catechin inhibits intestinal tumor formation and suppresses Focal adhesion kinase activation in the Min/+ Mouse[7].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: LB02 tumor cell line
  • Concentrations: 10, 50, 100 or 200 μg/mL
  • Incubation Time: 48 h
  • Method:

    LB02 cells were resuspended in RPMI 10% FBS in a concentration of 5x105 cells/mL. The cultures were performed in 96-well round-bottomed microtitre plates in presence of different concentrations of catechin or rutin (10, 50, 100 or 200 μg/mL). After 48 h at 37 ºC in a humidified atmosphere with 5% CO2 in the air, cultures were pulsed with 1 μCi of 3H-thymidine/well and maintained for an additional 18 h period prior to a semiautomatic method harvest.

Animal Research:

[4]

  • Animal Models: Kunming mice
  • Dosages: 1, 2, 3 mg/kg
  • Administration: stomach perfusion

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 290.27
Formula

C15H14O6

CAS No. 154-23-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C=C3)O)O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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