ML385

For research use only.

Catalog No.S8790

33 publications

ML385 Chemical Structure

CAS No. 846557-71-9

ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.

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Selleck's ML385 has been cited by 33 publications

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Choose Selective Nrf2 Inhibitors

Biological Activity

Description ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.
Targets
Ferroptosis [3]
()
Nrf2 [1]
()
1.9 μM
In vitro

ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination with carboplatin. The PK profile tested in CD-1 mice shows that ML385 has a half-life (t1/2 = 2.82 h) after IP injection (30 mg/kg)[1]. ML385 could alleviate the pancreatic injury[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MG-63 cells MXrGeY5kfGmxbjDhd5NigQ>? MlrtNkDPxE1? Mmm4NkBpKGGwZDC0JIg> MV3NUFM5PSBqMjFCuY1wdC:OKTDjc5Vt\CC|aXfubYZq[2GwdHz5JIlv[3KnYYPlJINmdGy3bHHyJHJQWyCuZY\lcEBjgSC-MvMAl|Qu\m:uZDDpckBOTy14MzDj[YxteyCjZoTldkBkdy2rbnP1ZoF1cW:wIDj3bZRpKGe{YYDo[Y5mKG:6aXTlLS=> NF7LNIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi0OFY4Oyd-Mkm4OFQ3PzN:L3G+
A549 M2XzfWZ2dmO2aX;uJIF{e2G7 NYHzXXExOCxiNTDhcoQhOTBizszN MoiyNlQh[W6mIES4JIg> MYTBOVQ6KGOnbHzzJJRz\WG2ZXSge4l1cCCPTEO4OUBl\W2xboP0doF1\WRiYTDkc5NmNWSncHXu[IVvfCC{ZXT1Z5Rqd25iaX6gUnJHOiCycn;0[YlvKGyndnXsd{BidmRiaYTzJJRiemendDDn[Y5mew>? NIfrfGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{W1NlM{QSd-Mke1OVI{Ozl:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Nrf2 ; 

PubMed: 31456942     


ML385, a specific inhibitor of Nrf2, was added into A2780 cells with terminal concentration of 5 μM for 24 or 48 h and the inhibition efficiency was measured by western blot. 

31456942
Immunofluorescence
Nrf2; 

PubMed: 31089407     


Immunofluorescence analysis of Nrf2 expression and nuclear translocation. Green fluorescence represents Nrf2, and blue fluorescence represents nucleus of hDPCs. 

31089407

Protocol

Cell Research:

[1]

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  • Cell lines: A549 cells
  • Concentrations: 5 μM
  • Incubation Time: 24, 48 and 72 h
  • Method:

    --


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: male CD-1 mice
  • Dosages: 30 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 74 mg/mL (144.64 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 511.59
Formula

C29H25N3O4S

CAS No. 846557-71-9
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC=CC=C1C(=O)N2CCC3=C2C=CC(=C3)C4=C(SC(=N4)NC(=O)CC5=CC6=C(C=C5)OCO6)C

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID