ML385

Catalog No.S8790

For research use only.

ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.

ML385 Chemical Structure

CAS No. 846557-71-9

Selleck's ML385 has been cited by 65 publications

Purity & Quality Control

Choose Selective Nrf2 Inhibitors

Biological Activity

Description ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.
Targets
Ferroptosis [3] Nrf2 [1]
1.9 μM
In vitro

ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination with carboplatin. The PK profile tested in CD-1 mice shows that ML385 has a half-life (t1/2 = 2.82 h) after IP injection (30 mg/kg)[1]. ML385 could alleviate the pancreatic injury[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MG-63 cells NHTDPHhHfW6ldHnvckBie3OjeR?= NEm0WZYzKM7:TR?= NVP3V2hROiCqIHHu[EA1KGh? NEK4T2lOVDN6NTCoNkDDvW2xbD;MLUBkd3WuZDDzbYdvcW[rY3HueIx6KGmwY4LlZZNmKGOnbHz1cIFzKFKRUzDs[ZZmdCCkeTD+NwKBmzRvZn;s[EBqdiCPRz22N{Bk\WyuczDh[pRmeiClbz3pcoN2[mG2aX;uJEh4cXSqIHfyZZBp\W6nIH;4bYRmMQ>? NHTNfYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi0OFY4Oyd-Mkm4OFQ3PzN:L3G+
A549 MkLiSpVv[3Srb36gZZN{[Xl? MXqwMEA2KGGwZDCxNEDPxE1? MYmyOEBidmRiNEigbC=> MWDBOVQ6KGOnbHzzJJRz\WG2ZXSge4l1cCCPTEO4OUBl\W2xboP0doF1\WRiYTDkc5NmNWSncHXu[IVvfCC{ZXT1Z5Rqd25iaX6gUnJHOiCycn;0[YlvKGyndnXsd{BidmRiaYTzJJRiemendDDn[Y5mew>? M3\NWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUWyN|M6Lz5{N{W1NlM{QTxxYU6=
Assay
Methods Test Index PMID
Western blot Nrf2 31456942
Immunofluorescence Nrf2 31089407

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: A549 cells
  • Concentrations: 5 μM
  • Incubation Time: 24, 48 and 72 h
  • Method:

    --

Animal Research:

[1]

  • Animal Models: male CD-1 mice
  • Dosages: 30 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 511.59
Formula

C29H25N3O4S

CAS No. 846557-71-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CC=CC=C1C(=O)N2CCC3=C2C=CC(=C3)C4=C(SC(=N4)NC(=O)CC5=CC6=C(C=C5)OCO6)C

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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