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ML385 Nrf2 inhibitor

Name: ML385
Brand: Selleck Chemicals
SKU: S8790
Availability: InStock
Rating: 5 (149 reviews)

Cat.No.S8790

ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. This compound regulates the activity of several ferroptosis and lipid peroxidation-related proteins.

Chemical Structure

Molecular Weight: 511.59

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.92%

99.92

Products Often Used Together with ML385

Brusatol

It and Brusatol use significantly reduces ABCC5 expression and downregulates NRF2 expression in Sk-Hep-1 cells.

RSL3

When used together with RSL3, it synergistically and significantly inhibits cell proliferation in MV4;11/Kasumi-1/KG1α cells.

Related Targets	Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor TNF-alpha ROS Parasite PD-1/PD-L1 TLR Prostaglandin Receptor IFN CXCR NOS CCR NLRP3 eIF STING AhR IL Receptor Complement System IRAK β-lactamase IDO/TDO PGES NADPH-oxidase FKBP LTR
Related Products	Bardoxolone Methyl TBHQ Brusatol Oltipraz Bardoxolone (CDDO) 4-Octyl Itaconate CDDO-Im (RTA-403) cis-Resveratrol Mangiferin

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MG-63 cells	Function assay	2 μM	2 h and 4 h	ML385 (2 µmol/L) could significantly increase cellular ROS level by ~2–4-fold in MG-63 cells after co-incubation (with graphene oxide)	29844673
A549	Function assay	0, 5 and 10 μM	24 and 48 h	A549 cells treated with ML385 demonstrated a dose-dependent reduction in NRF2 protein levels and its target genes	27552339
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	511.59	Formula	C₂₉H₂₅N₃O₄S	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	846557-71-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC1=CC=CC=C1C(=O)N2CCC3=C2C=CC(=C3)C4=C(SC(=N4)NC(=O)CC5=CC6=C(C=C5)OCO6)C

Solubility

In vitro
Batch:

DMSO : 20 mg/mL ( (39.09 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	6%DMSO 1 40%PEG300 2 5%Tween80 3 49%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.500mg/ml (2.93mM)	Taking the 1 mL working solution as an example, add 60 μL 25 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 490 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	6%DMSO 1 40%PEG300 2 5%Tween80 3 49%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.500mg/ml (2.93mM)	Taking the 1 mL working solution as an example, add 60 μL 25 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 490 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	10% DMSO 1 40% PEG 300 2 5%Tween80 3 45%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.000mg/ml (5.86mM)	Taking the 1 mL working solution as an example, add 100 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 450 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Ferroptosis ^[3] Nrf2 ^[1] 1.9 μM
In vitro	ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination. The PK profile tested in CD-1 mice shows that this compound has a half-life (t_1/2 = 2.82 h) after IP injection (30 mg/kg)^[1]. This chemical could alleviate the pancreatic injury^[2].
References	https://pubmed.ncbi.nlm.nih.gov/27552339/ https://www.hindawi.com/journals/omcl/2018/7161592/ https://pubmed.ncbi.nlm.nih.gov/32907551/

Applications

Methods	Biomarkers	Images	PMID
Western blot	Nrf2		31456942
Immunofluorescence	Nrf2		31089407

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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