CDDO-Im

For research use only.

Catalog No.S9723 Synonyms: CDDO-Imidazolide, RTA-403, TP-235

CDDO-Im Chemical Structure

CAS No. 443104-02-7

CDDO-Im (CDDO-Imidazolide, RTA-403, TP-235) is an activator of nuclear factor erythroid 2–related factor 2 (Nrf2) and peroxisome proliferator-activated receptor (PPAR). CDDO-Im binds to PPARα and PPARγ with Ki of 232 nM and 344 nM, respectively. CDDO-Im inhibits inflammatory response and tumor growth in vivo.

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Biological Activity

Description CDDO-Im (CDDO-Imidazolide, RTA-403, TP-235) is an activator of nuclear factor erythroid 2–related factor 2 (Nrf2) and peroxisome proliferator-activated receptor (PPAR). CDDO-Im binds to PPARα and PPARγ with Ki of 232 nM and 344 nM, respectively. CDDO-Im inhibits inflammatory response and tumor growth in vivo.
Targets
Nrf2 [1]
()
PPARα [1]
(Cell-free assay)
PPARγ [1]
(Cell-free assay)
232 nM(Ki) 344 nM(Ki)
In vitro

CDDO-Im is highly active in suppressing cellular proliferation of human leukemia and breast cancer cell lines (IC50, 10–30 nM). In U937 leukemia cells, CDDO-Im also induces monocytic differentiation as measured by increased cell surface expression of CD11b and CD36. CDDO-Im has significant actions independent of PPAR transactivation.[2]

In vivo

CDDO-Im is a novel synthetic triterpenoid more potent than its parent compound (CDDO). In vivo, CDDO-Im is a potent inhibitor of de novo inducible nitric oxide synthase expression in primary mouse macrophages. Moreover, CDDO-Im inhibits growth of B16 murine melanoma and L1210 murine leukemia cells in vivo.[2]

Protocol

Cell Research:

[2]

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  • Cell lines: U937, THP-1, and HL-60 leukemia cells
  • Concentrations: 100 pM to 1 μM
  • Incubation Time: 5 days
  • Method:

    U937, THP-1, and HL-60 leukemia cells are treated with either control vehicle, CDDO, or CDDO-Im at concentrations ranging from 100 pM to 1 μM, and after 5 days, proliferation is measured by cell counting.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: female CD-1 mice, male and female BDF-1 mice
  • Dosages: 1 nmole, 10 nmole
  • Administration: IP
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 56 mg/mL (103.37 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 541.72
Formula
C34H43N3O3
CAS No. 443104-02-7
Storage powder
in solvent
Synonyms CDDO-Imidazolide, RTA-403, TP-235

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID