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RSL3 Ferroptosis inducer

Name: RSL3
SKU: S8155
Availability: InStock
Rating: 5 (474 reviews)

Cat.No.S8155

RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.

Chemical Structure

Molecular Weight: 440.88

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.97%

99.97

Products Often Used Together with RSL3

Erastin

This compound and Erastin are ferroptosis inducers (FINs) and sensitize cancer cells (H460/A549) to ionizing radiation (IR).

Ferrostatin-1 (Fer-1)

Ferrostatin-1 (Fer-1) efficiently abrogates the rise in reactive oxygen species (ROS) levels caused by this compound in M0/M1/M2 macrophages.

Liproxstatin-1

This compound-induced lipid oxidation is prevented by Liproxstatin-1 in human renal proximal tubular epithelial cells (HRPTEpiCs).

FIN56

This compound and FIN56 are ferroptosis inducers that cause a decrease in GPX4 protein abundance in HT-1080 cells.

ML 210

This compound and ML 210 induce lipid reactive oxygen species (ROS) and iron-dependent cell death in small-cell lung cancer (SCLC).

Related Targets	P450 (e.g. CYP17) Dehydrogenase PDE phosphatase PPAR HSP Vitamin Transferase Carbohydrate Metabolism Mitochondrial Metabolism Casein Kinase Serine/threonin kinase Lipoxygenase Phospholipase (e.g. PLA) Retinoid Receptor DPP ACE Peroxidases Amino Acids and Derivatives Fatty Acid Synthase FXR HMG-CoA Reductase Carbonic Anhydrase Lipase Decarboxylase DHFR IDO/TDO Hydroxylase PCSK9 OXPHOS
Related Products	Ferrostatin-1 (Fer-1) Erastin Liproxstatin-1 FIN56 IKE (Imidazole Ketone Erastin) UAMC-3203 iFSP1 SRS16-86 SRS11-92 Erastin2 NPD4928 icFSP1 Ferric citrate Butylhydroxyanisole GPX4-IN-5 FSEN1 N6F11

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MEFs and HT1080 cells	Function assay	0.5 μM	24 h		30686534
Jurkat	Cell death assay	0.1 μM	24 h or 48 h	BV6 cooperates with RSL3 to induce cell death, accompanied by ROS production and lipid peroxidation	27588473
Molt-4	Cell death assay	0.075 μM	24 h or 48 h	BV6 cooperates with RSL3 to induce cell death, accompanied by ROS production and lipid peroxidation	27588473
RMS13 cells	Cell death assay	0, 60, 100, 140 and 180 μM	48 h	Addition of Ferrostatin-1 significantly reduced RSL3- or Erastin-induced loss of cell viability.	26157704
BJeLR	Cytotoxicity assay	0.57 uM	12 h	Cytotoxicity against human BJeLR cells expressing RAS G12V mutant at 0.57 uM at 12 hrs by trypan blue staining	22832321
BJeLR	Cytotoxicity assay	0.57 uM	24 h	Cytotoxicity against human BJeLR cells expressing RAS G12V mutant at 0.57 uM at 24 hrs by trypan blue staining	22832321
BJeH	Function assay		6 h	Induction of reactive oxygen species production in human BJeH cells expressing wild type RAS after 6 hrs by DCF-based flow cytometric analysis	22832321
BJeLR	Function assay		6 h	Induction of reactive oxygen species production in human BJeLR cells expressing RAS G12V mutant after 6 hrs by DCF-based flow cytometric analysis	22832321
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	440.88	Formula	C₂₃H₂₁ClN₂O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1219810-16-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	(1S,3R)-RSL3			Smiles	COC(=O)C1CC2=C(C(N1C(=O)CCl)C3=CC=C(C=C3)C(=O)OC)NC4=CC=CC=C24

Solubility

In vitro
Batch:

DMSO : 88 mg/mL (199.6 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 28 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.000mg/ml (9.07mM)	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (0.57mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Gpx4 ^[1] (In Calu-1 cells)
In vitro	Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, this compound inactivates GPX4 to induce ROS production from lipid peroxidation. Its ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment^[1]^[2].
In vivo	Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and this compound in mice^[1].
References	[1] https://pubmed.ncbi.nlm.nih.gov/18355723/ [2] https://pubmed.ncbi.nlm.nih.gov/26794443/

Applications

Methods	Biomarkers	PMID
Western blot	GPX4 / Tranferrin / Ferritin HO-1	30524291
Growth inhibition assay	Cell viability	26157704
Immunofluorescence	ALOX12 / ALOX15 4-HNE	28837253

Tech Support

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