ML-SA1

Catalog No.S9926 Synonyms: Mucolipin synthetic agonist 1

For research use only.

ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.

ML-SA1 Chemical Structure

CAS No. 332382-54-4

Purity & Quality Control

Choose Selective TRP Channel Inhibitors

Biological Activity

Description

ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.

Targets
DENV2 [2]
(A549 cells cells)
TRPML channel [1] ZIKV [2]
(A549 cells cells)
8.93 µM 52.99 µM
In vitro

ML-SA1, as a potent inhibitors of DENV2 and ZIKV, can inhibit DENV2 and ZIKV by promoting lysosome acidification and protease activity to cause viral degradation.<sup><a class="sref" href="#s_ref">[2]</a></sup>

In vivo

Administration of ML-SA1 before transient global ischemia ameliorated neurological dysfunction possibly through the promotion of autophagy and the inhibition of apoptosis.<sup><a class="sref" href="#s_ref">[3]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[2]</a></sup>

  • Cell lines: A549 cells, Huh7 cells
  • Concentrations: 25 μM
  • Incubation Time: 0-14 h
  • Method:

    A549 or Huh7 cells are seeded into 96-well plates at a density of 8 × 10<sup>3</sup> for 12 h, followed by incubation with various concentrations of eML-SA1. After 48 h of incubation, the cell are used for CCK-8 assay. A549 or Huh7 cells are seeded into 12-well plates at a density of 2 × 105 for 12 h and pretreated with 25 μM ML-SA1 for 24 h using DMSO as a control. Then, cells are incubated with 1 μM Lyso-Sensor Green DND-189 for 1 h at 37℃ and digested with 0.25% trypsin to generate a single cell suspension, which is used for Flow Cytometer to investigate the effect of ML-SA1 on lysosomal acidification.

Animal Research:

<sup><a class="sref" href="#s_ref">[3]</a></sup>

  • Animal Models: Transient global ischemia model mice (male; C57BL/6)
  • Dosages: 0.3 μl/min
  • Administration: i.c.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 362.42
Formula

C22H22N2O3

CAS No. 332382-54-4
Storage 3 years -20°C powder
2 years -80°C in solvent

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