Metyrapone

Catalog No.S5416 Synonyms: NSC-25265, SU-4885

For research use only.

Metyrapone (NSC-25265, SU-4885) is an inhibitor of the enzyme steroid 11-beta-monooxygenase (CYP11B1) that inhibits adrenal steroid synthesis.

Metyrapone Chemical Structure

CAS No. 54-36-4

Purity & Quality Control

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Biological Activity

Description Metyrapone (NSC-25265, SU-4885) is an inhibitor of the enzyme steroid 11-beta-monooxygenase (CYP11B1) that inhibits adrenal steroid synthesis.
Targets
11β-HSD1 [1]
()
30 μM(Ki)
In vitro

Metyrapone is a well known inhibitor of 11β-hydroxylase, a key enzyme involved in the final step of cortisol biosynthesis. At higher concentrations, it also inhibits other steroidogenic enzymes such as cholesterol side chain cleavage enzyme. It is specific for 11β-HSD1 while it has little effect on 11β-HSD2 activity[1].

In vivo The administration of metyrapone to man, and animals such as the dog and sheep thus leads to a decrease in circulating levels of cortisol[1].

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 46 mg/mL
(203.29 mM)
Ethanol ''-1 mg/mL

Chemical Information

Molecular Weight 226.27
Formula

C14H14N2O

CAS No. 54-36-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C1=CN=CC=C1)C(=O)C2=CN=CC=C2

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03491696 Recruiting Drug: Metyrapone Depressive Disorder|Hormone Disturbance Centre Hospitalier Rouffach|University Hospital Strasbourg France December 22 2018 Phase 4
NCT02406066 Completed Drug: metyrapone & oxazepam|Drug: Placebo Cocaine Use Disorder|Tobacco Use Disorder Embera NeuroTherapeutics Inc.|National Institute on Drug Abuse (NIDA) March 2015 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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