Methyl salicylate

Catalog No.S3756 Synonyms: 2-Carbomethoxyphenol, Betula oil, Gaultheria oil, Methyl 2-hydroxybenzoate, Natural wintergreen oil

For research use only.

Methyl salicylate (Betula oil, Gaultheria oil, Methyl 2-hydroxybenzoate, Natural wintergreen oil) is an organic ester naturally produced by many species of plants, particularly wintergreens. It is an agonist for TRPA1 and TRPV1.

Methyl salicylate Chemical Structure

CAS No. 119-36-8

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Biological Activity

Description Methyl salicylate (Betula oil, Gaultheria oil, Methyl 2-hydroxybenzoate, Natural wintergreen oil) is an organic ester naturally produced by many species of plants, particularly wintergreens. It is an agonist for TRPA1 and TRPV1.
Targets
TRPA1 [1]
(Cell-free assay)
TRPV1 [1]
(Cell-free assay)
In vitro

In human embryonic kidney 293 cells expressing human TRPV1 (hTRPV1), Methyl salicylate evokes increases of [Ca2+]i, which declines regardless of its continuous presence, indicative of marked desensitization. Methyl salicylate promotes Ca2+ influx through the activation of TRPV1 channels and reversibly inhibits hTRPV1 activation by polymodal stimuli such as capsaicin, protons, heat, anandamide, and 2-aminoethoxydiphenyl borate. Methyl salicylate is an effective agonist for recombinant human and porcine TRPV1[1].

In vivo Methyl salicylate significantly suppresses nocifensive behavior induced by intraplantar capsaicin in rats[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HEK 293 cells
  • Concentrations: 3 mM
  • Incubation Time: --
  • Method: Fura-2-loaded hTRPV1-expressing HEK 293 cells are first stimulated with capsaicin (30 nM) and subsequently with Methyl salicylate (3 mM).
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: Male Wistar rats (8 weeks old)
  • Dosages: 0.5 mg
  • Administration: injected into the same hind paw
  • (Only for Reference)

Chemical Information

Molecular Weight 152.15
Formula

C8H8O3

Density 1.174 g/mL at 25 °C
CAS No. 119-36-8
Storage 2 years -20°C liquid
Smiles COC(=O)C1=CC=CC=C1O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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