Garlic oil

Catalog No.E0651

For research use only.

Garlic oil blocks tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis by inducing phase II drug-metabolizing enzymes.

CAS No. 8000-78-0

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Biological Activity

Description Garlic oil blocks tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis by inducing phase II drug-metabolizing enzymes.
In vitro

Garlic oil exhibits favorable anti‑cancer potential towards gliomas of various degrees of differentiation. Garlic oil induces apoptotic death in Human astrocytoma cell lines at a low concentration(0.75 µg/ml-1.5 µg/ml). [2]

In vivo

Garlic oil supplementation during pregnancy and lactation protects against hypertension induced by high-fat (HF) diet in adult male offspring. The beneficial effects of garlic oil are associated with increased renal mRNA expression and activity of Hydrogen sulfide (H2 S)-generating enzymes, increased NO bioavailability, increased plasma short chain fatty acid levels, and alterations of gut microbiota composition. Garlic oil supplementation increases abundance of genus Lactobacillus, but decreases genera Turicibacter and Staphylococcus.[3]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Human astrocytoma cell lines
  • Concentrations: 0.015 µg/ml-150 µg/ml
  • Incubation Time: 24 h
  • Method:

    Human astrocytoma cell lines are incubated with 15 or 150 µg/ml garlic oil for 24 h, and then used for the MTT assay to determine viability of cells. Annexin V conjugated to fluorescein isothiocyanate (FITC) fluorochrome is used for flow cytometry analysis of cells which are undergoing apoptosis.

Animal Research:

[3]

  • Animal Models: Pregnant rats
  • Dosages: 100 mg/kg
  • Administration: o.g.

Chemical Information

Molecular Weight
Formula

N/A

CAS No. 8000-78-0
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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