Home Immunology & Inflammation TLR antagonist CU-CPT-8m For research use only.

CU-CPT-8m

Synonyms: CPD1578

CU-CPT-8m (CPD1578) is a specific antagonist of toll-like receptor 8(TLR8) with IC50 of 67 nM and Kd of 220 nM.

CU-CPT-8m Chemical Structure

CU-CPT-8m Chemical Structure

CAS: 125079-83-6

Purity & Quality Control

Batch: S446101 DMSO] 6 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.98%
99.98

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Biological Activity

Description CU-CPT-8m (CPD1578) is a specific antagonist of toll-like receptor 8(TLR8) with IC50 of 67 nM and Kd of 220 nM.
Targets
TLR8 [1]
(Cell-free assay)		 TLR8 [1]
(Cell-free assay)
67 nM 220 nM(Ki)
In vitro
In vitro

CU-CPT8m, recognizes a pocket on the interface of two copies of TLR8 and prevents them from undergoing conformational changes that are necessary for the proinflammatory signaling.[2]
Cell Research Cell lines HEK-Blue TLR8 cells
Concentrations 24 h
Incubation Time 0.1 nM-10 mM
Method

HEK-Blue TLR8 cells were seeded in tissue culture treated 96-well plates with a density of 7.5 × 104 cells/well in DMEM media supplemented with 10% FBS (phosphatases deactivated with heat). These cells were then treated with R848 (1 μg/mL) along with various concentrations of appropriate compounds. Plates are then incubated in humidified incubators containing 5% CO2 at 37 °C for 24 h. Supernatant was removed, and 100 µL of 1:10 diluted WST-1 (Roche) was then added to each well. The plate was then incubated at 37 ℃ till color change (30 min-1 h).

Chemical Information & Solubility

Molecular Weight 252.27 Formula

C14H12N4O
CAS No. 125079-83-6 SDF --
Smiles CC1=CC(=CC=C1)C2=CC=NC3=C(C=N[N]23)C(N)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 6 mg/mL ( (23.78 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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