CU-CPT-8m

Catalog No.S4461 Batch:S446101

Technical Data

Formula	C₁₄H₁₂N₄O
Molecular Weight	252.27	CAS No.	125079-83-6
Solubility (25°C)*	In vitro	DMSO	6 mg/mL (23.78 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

CU-CPT-8m (CPD1578) is a specific antagonist of toll-like receptor 8(TLR8) with IC50 of 67 nM and Kd of 220 nM.

Targets

TLR8 ^[1] (Cell-free assay)	TLR8 ^[1] (Cell-free assay)
67 nM	220 nM(Ki)

In vitro

CU-CPT8m, recognizes a pocket on the interface of two copies of TLR8 and prevents them from undergoing conformational changes that are necessary for the proinflammatory signaling.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HEK-Blue TLR8 cells Concentrations 24 h Incubation Time 0.1 nM-10 mM Method HEK-Blue TLR8 cells were seeded in tissue culture treated 96-well plates with a density of 7.5 × 10⁴ cells/well in DMEM media supplemented with 10% FBS (phosphatases deactivated with heat). These cells were then treated with R848 (1 μg/mL) along with various concentrations of appropriate compounds. Plates are then incubated in humidified incubators containing 5% CO2 at 37 °C for 24 h. Supernatant was removed, and 100 µL of 1:10 diluted WST-1 (Roche) was then added to each well. The plate was then incubated at 37 ℃ till color change (30 min-1 h).

Cell Assay:^{^[2]}

Cell lines
HEK-Blue TLR8 cells
Concentrations
24 h
Incubation Time
0.1 nM-10 mM
Method
HEK-Blue TLR8 cells were seeded in tissue culture treated 96-well plates with a density of 7.5 × 10⁴ cells/well in DMEM media supplemented with 10% FBS (phosphatases deactivated with heat). These cells were then treated with R848 (1 μg/mL) along with various concentrations of appropriate compounds. Plates are then incubated in humidified incubators containing 5% CO2 at 37 °C for 24 h. Supernatant was removed, and 100 µL of 1:10 diluted WST-1 (Roche) was then added to each well. The plate was then incubated at 37 ℃ till color change (30 min-1 h).

References

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SHIPPING AND STORAGE
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