Home Metabolism Dehydrogenase inhibitor CBR-5884 For research use only.

CBR-5884

CBR-5884 is a selective inhibitor of phosphoglycerate dehydrogenase (PHGDH) with IC50 of 33 μM. CBR-5884 inhibits de novo serine synthesis in cancer cells and was selectively toxic to cancer cell lines with high serine biosynthetic activity.

CBR-5884 Chemical Structure

CBR-5884 Chemical Structure

CAS: 681159-27-3

Purity & Quality Control

Batch: S964501 DMSO] 67 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.81%
99.81

CBR-5884 Related Products

Signaling Pathway

Dehydrogenase Signaling Pathways

Dehydrogenase Signaling Pathway

Choose Selective Dehydrogenase Inhibitors

Biological Activity

Description CBR-5884 is a selective inhibitor of phosphoglycerate dehydrogenase (PHGDH) with IC50 of 33 μM. CBR-5884 inhibits de novo serine synthesis in cancer cells and was selectively toxic to cancer cell lines with high serine biosynthetic activity.
Targets
PHGDH [1]
(Cell-free assay)
33 μM
In vitro
In vitro

CBR-5884 inhibits de novo serine synthesis in cancer cells and is selectively toxic to cancer cell lines with high serine biosynthetic activity. Biochemical characterization of the inhibitor reveals that it is a noncompetitive inhibitor that shows a time-dependent onset of inhibition and disrupts the oligomerization state of PHGDH.[1]
Cell Research Cell lines Carney cells
Concentrations 1 μM, 15 μM, 30 μM; 1 μM to 40 μM
Incubation Time 1 h; 3 h
Method

Acute Toxicity Assay. Carney cells acclimated to growth in MEM media are plated in a 96-well plate at 6,000 cells per well. The next day, cells are treated with CBR-5884 from 1 μM to 40 μM for 3 h. Drug containing media are then removed, fresh drug-free media added, and cell viability is determined via a CellTiter-Glo or Alamar Blue assay according to the manufacturer’s protocol.

Chemical Information & Solubility

Molecular Weight 336.39 Formula

C14H12N2O4S2
CAS No. 681159-27-3 SDF --
Smiles CCOC(=O)C1=C(C(=C(S1)NC(=O)C2=CC=CO2)SC#N)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.17 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy CBR-5884 | CBR-5884 supplier | purchase CBR-5884 | CBR-5884 cost | CBR-5884 manufacturer | order CBR-5884 | CBR-5884 distributor