Bromodeoxyuridine (BrdU)

For research use only. Not for use in humans.

Catalog No.S7918

4 publications

Bromodeoxyuridine (BrdU) Chemical Structure

Molecular Weight(MW): 307.1

Bromodeoxyuridine (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.

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Selleck's Bromodeoxyuridine (BrdU) has been cited by 4 publications

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  • Immunofluorescence microphotographs of proliferating ARPE-19 cell with BrdU (red) and DAPI (blue) staining after ARPE-19 cells were untreated or treated with AF (1.0 μM) in the absence or presence of EGF (100ng/ml) for 24 hours and then subjected to BrdU labeling for 4 hours, followed by immunostaining with anti-BrdU antibody and DAPI. Scale bar = 20μm.

    PLoS One, 2016, 11(11):e0166386.. Bromodeoxyuridine (BrdU) purchased from Selleck.

    Immunofluorescence microphotographs of proliferating ARPE-19 cell with BrdU (red) and DAPI (blue) staining after ARPE-19 cells were untreated or treated with AF (1.0 μM) in the absence or presence of EGF (100ng/ml) for 24 hours and then subjected to BrdU labeling for 4 hours, followed by immunostaining with anti-BrdU antibody and DAPI. Scale bar = 20μm.

    PLoS One, 2016, 11(11):e0166386. Bromodeoxyuridine (BrdU) purchased from Selleck.

  • Immunofluorescence microphotographs of proliferating ARPE-19 cells with BrdU (red) and DAPI (blue) staining were taken after ARPE-19 cells were left untreated or treated with H2O2 (300 µmol/L) in the absence or presence of EGF (100 ng/mL) for 24h and then subjected to BrdU labeling for 4h, followed by immunostaining with anti-BrdU antibody and DAPI.

    Int J Ophthalmol, 2017, 10(4):507-514. Bromodeoxyuridine (BrdU) purchased from Selleck.

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Biological Activity

Description Bromodeoxyuridine (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.
In vitro

In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. [1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. [2]

In vivo In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. [1]

Protocol

Cell Research:[1]
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  • Cell lines: RG2 rat glioma cells
  • Concentrations: ~ 50 µM
  • Incubation Time: 24 hours
  • Method: Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.
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Solubility (25°C)

In vitro DMSO 61 mg/mL (198.63 mM)
Ethanol 2 mg/mL warmed (6.51 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 307.1
Formula

C9H11BrN2O5

CAS No. 59-14-3
Storage powder
in solvent
Synonyms N/A
Smiles OCC1OC(CC1O)N2C=C(Br)C(=O)NC2=O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID