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BrdU (Bromodeoxyuridine) DNA/RNA Synthesis chemical

Name: BrdU (Bromodeoxyuridine)
Brand: Selleck Chemicals
SKU: S7918
Availability: InStock
Rating: 5 (17 reviews)

Cat.No.S7918

BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and it is used in the detection of proliferating cells.

Chemical Structure

Molecular Weight: 307.1

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.99%

99.99

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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Activity Description	PMID
mouse neural precursor cells	Antiproliferative assay	Antiproliferative activity against mouse neural precursor cells by MTT assay, EC50=2.045μM	17417631
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of FdUrd resistant L5178Y-Resistant murine leukemia cells, IC50=12μM	11101356
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]Leu., IC50=20μM	11101356
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of L5178Y-Parental murine leukemia cells, IC50=26μM	11101356
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd., IC50=26μM	11101356
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	307.1	Formula	C₉H₁₁BrN₂O₅	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
CAS No.	59-14-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	5-Bromo-2'-deoxyuridine, BUdR			Smiles	C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O

Solubility

In vitro
Batch:

DMSO : 61 mg/mL (198.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	4%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	7.500mg/ml (24.42mM)	Taking the 1 mL working solution as an example, add 40 μL of 187.5 mg/ml clear DMSO stock solution to 960 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (32.56mM)	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.63mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	Bromodeoxyuridine (BrdU) induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion in RG2 rat glioma cells, and alters the cell cycle profile in H9 cells and BJ fibroblasts. ^[1] This compound is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. ^[2]
In vivo	In rat glioma RG2 tumor model, BrdU (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. ^[1]
References	[1] https://pubmed.ncbi.nlm.nih.gov/18680882/ [2] https://pubmed.ncbi.nlm.nih.gov/18770853/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04365205	Recruiting	Asthma	University Hospital Bordeaux	September 23 2020	--
NCT02654925	Completed	Obesity	University of Colorado Denver	March 2016	--
NCT01407211	Unknown status	Relapsing Remitting Multiple Sclerosis	Tehran University of Medical Sciences	April 2011	Phase 4
NCT01414972	Unknown status	Atherosclerosis	Tehran University of Medical Sciences	May 2010	Phase 4
NCT01225289	Completed	Relapsing Remitting Multiple Sclerosis	Tehran University of Medical Sciences	October 2009	Phase 4
NCT00032344	Completed	Colorectal Cancer	US Department of Veterans Affairs\|VA Office of Research and Development	October 1993	Phase 3

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