Bromodeoxyuridine (BrdU)

Catalog No.S7918 Synonyms: 5-Bromo-2'-deoxyuridine, BUdR

For research use only.

Bromodeoxyuridine (BrdU, 5-Bromo-2'-deoxyuridine, BUdR) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.

Bromodeoxyuridine (BrdU) Chemical Structure

CAS No. 59-14-3

Selleck's Bromodeoxyuridine (BrdU) has been cited by 9 Publications

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Biological Activity

Description Bromodeoxyuridine (BrdU, 5-Bromo-2'-deoxyuridine, BUdR) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.
In vitro

In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. [1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse neural precursor cells M3PwW2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NInFeIJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIH7leZJidCCycnXjeZJ{d3JiY3XscJMh[nliTWTUJIF{e2G7LDDFR|UxRTJwMES1{txO MlLTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N|EoRjF5NEG3OlMyRC:jPh?=
murine leukemia cells MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4\TcWlvKH[rdILvJIdzd3e2aDDpcohq[mm2aX;uJI9nKE[mVYLkJJJme2m|dHHueEBNPTF5OGmtVoV{cXO2YX70JI12emmwZTDs[ZVs\W2rYTDj[YxteyxiSVO1NF0yOs7:TR?= MoTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTFzMEGzOVYoRjFzMUCxN|U3RC:jPh?=
murine leukemia cells MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIHhNGFKdiC4aYTyc{Boem:5dHigbY5pcWKrdHnvckBw\iCONUG3PHkuWGG{ZX70ZYwhdXW{aX7lJIxmfWunbXnhJINmdGy|IHL5JIlv[2:{cH;yZZRqd25ib3[gX|E1S12OZYWuMEBKSzVyPUKw{txO MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTFyMUO1Okc,OTFzMEGzOVY9N2F-
murine leukemia cells MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1vHW2lvKH[rdILvJIdzd3e2aDDpcohq[mm2aX;uJI9nKEx3MUe4XU1R[XKnboThcEBufXKrbnWgcIV2c2WvaXGgZ4VtdHNuIFnDOVA:OjcQvF2= NVS4VJlXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGxNFE{PTZpPkGxNVAyOzV4PD;hQi=>
murine leukemia cells M3K0eGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MleyTY4hfmm2cn:g[5Jwf3SqIHnubIljcXSrb36gc4YhVDVzN{jZMXBiemWwdHHsJI12emmwZTDs[ZVs\W2rYTDj[YxteyCkeTDpcoNwenCxcnH0bY9vKG:oIGuxOGNeNVSqZD6sJGlEPTB;MkdOwG0> M2H4NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMUCxN|U3Lz5zMUGwNVM2PjxxYU6=
In vivo In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. [1]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: RG2 rat glioma cells
  • Concentrations: ~ 50 µM
  • Incubation Time: 24 hours
  • Method: Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 307.1


CAS No. 59-14-3
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Smiles C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04365205 Recruiting Procedure: Endobronchial biopsy Asthma University Hospital Bordeaux September 23 2020 --
NCT02654925 Completed Procedure: Adipose Tissue Biopsy Obesity University of Colorado Denver March 2016 --
NCT01407211 Unknown status Dietary Supplement: vitamin A Relapsing Remitting Multiple Sclerosis Tehran University of Medical Sciences April 2011 Phase 4
NCT01414972 Unknown status Drug: Vitamin A|Drug: placebo Atherosclerosis Tehran University of Medical Sciences May 2010 Phase 4
NCT01225289 Completed Dietary Supplement: Vitamin A|Drug: Placebo Relapsing Remitting Multiple Sclerosis Tehran University of Medical Sciences October 2009 Phase 4
NCT00032344 Completed Procedure: Colonoscopy Colorectal Cancer US Department of Veterans Affairs|VA Office of Research and Development October 1993 Phase 3

(data from, updated on 2022-08-01)

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