Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NVrTWYV4S3m2b4TvfIlkyqCjc4PhfS=> MX:3NkBp Moj1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDpZ|UxRTFibl2= NV\Kcml3OjB|N{GxPFM>
human Bel7402 cells MUDDfZRwfG:6aXRCpIF{e2G7 MXy5OkBp NIPN[JREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NECyJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS5{IH7N MVyxOVE3PTF2NB?=
human HCT8 cells NH7pWWVEgXSxdH;4bYPDqGG|c3H5 M{fpcVk3KGh? NXLwN2ZTS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvPSCwTR?= NWLp[pVQOTVzNkWxOFQ>
human MCF7 cells NI\Rd4lEgXSxdH;4bYPDqGG|c3H5 M1XO[Vk3KGh? MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT64JI5O NX3tTHk1OTVzNkWxOFQ>
human A549 cells NVW2fYx4S3m2b4TvfIlkyqCjc4PhfS=> NYjkWZZqQTZiaB?= NVXPUIlVS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVMvOiCwTR?= NVfTZXJpOTVzNkWxOFQ>
human colon carcinoma SW620 cell MUnDfZRwfG:6aXRCpIF{e2G7 MoDsR5l1d3SxeHnjJJBwfGWwdHnheIlwdiCxZjDUc5BwfGWlYX6gLHRRMSCkeTD0bIUh[2:vcH;1coQhcW5iaIXtZY4h[2:ub36gZ4Fz[2mwb33hJHNYPjJyIHPlcIwhdGmwZTygTWM2OD1|LkKgcm0> NGjl[ZgyOjRyOEewOy=>
human MESSA cells NXzlTGVYS3m2b4TvfIlkyqCjc4PhfS=> MnPvO|IhcA>? NXLicnJqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUWVU1GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVQhdk1? MoLBNlE{PDF4N{S=
human BGC823 cells MYPDfZRwfG:6aXRCpIF{e2G7 MYm5OkBp MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20MlMhdk1? NXzEWpFTOTVzNkWxOFQ>
human Ketr3 cells NFj1cVFEgXSxdH;4bYPDqGG|c3H5 Ml\yPVYhcA>? MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDL[ZRzOyClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUSuPUBvVQ>? M4Dnb|E2OTZ3MUS0
LOX cells MUTDfZRwfG:6aXRCpIF{e2G7 MkP0R5l1d3SxeHnjbZR6KHejczDk[ZRmem2rbnXkJIlvKH[rdILvJIlvKEyRWDDj[YxteyBqbXXsZY5wdWFrIH;mJIh2dWGwIIT1cY9zKGOnbHygcIlv\XNiYomgeZNqdmdiTWTUJIF{e2G7LDDJR|UxRTVibl2= MUK3PFU{OzNz
human A549 cells MXfGeY5kfGmxbjDhd5NigQ>? MVe0JIg> NWrGRmVySW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NTDuUS=> MVqxPFIxPzd2OB?=
human H69 cells MWfDfZRwfG:6aXRCpIF{e2G7 MofiO|IhcA>? MV;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIOlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTZibl2= MYGyNVM1OTZ5NB?=
human A2780 cells MmLhR5l1d3SxeHnjxsBie3OjeR?= MmjDO|IhcA>? M4rOZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJIFteGijNXLleIE{KGmwdHXndolvKGG|c3Xzd4VlKGG|IHPlcIwhe3W{dnn2ZYwh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME22JI5O MnSyNlI6PTl{NE[=
human LNCAP cells NH7ZfWFEgXSxdH;4bYPDqGG|c3H5 M2fWXlczKGh? M{HWeGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME25JI5O NWLXWpJIOjF|NEG2O|Q>
human HOP62 cells MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M37lN2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhQWDZ{IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= MXyyOVkxQTJ5OR?=
human MCF7 cells MknwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{n4[Wdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCLQ{WwQVExKG6P M{W2c|I2QTB7Mke5
human UACC62 cells M17Qb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M172cWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHVCS0N4MjDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= MVuyOVkxQTJ5OR?=
human DU145 cells NE\mZolIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV7Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDEWVE1PSClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEmFNUC9NVAhdk1? NWTqNmVPOjV7MEmyO|k>
LoVo cell line NV;3PG5PS3m2b4TvfIlkyqCjc4PhfS=> MYPDfZRwfG:6aXOgdI91\W62aXH0bY9vKG:oIGTvdI91\WOjbjCoWHAqKGK7IITo[UBkd22yb4Xu[EBqdiBqaIXtZY4h[2:ub4LlZ5RidCClYX7j[ZIhVG:YbzDj[YxtKGyrbnWsJGlEPTB;MUGuOkBvVQ>? M1fpcFEzPDB6N{C3
human lymphoblast tumor cell line RPM18402 NXq3NGxIS3m2b4TvfIlkyqCjc4PhfS=> M1jBUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx6dXCqb3LsZZN1KHS3bX;yJINmdGxibHnu[UBTWE1zOESwNkwhUUN3ME2xNkBvVQ>? NHLRWpMyOjN7Mke0OS=>
human HL60 cells NIfyWYlHfW6ldHnvckBie3OjeR?= NEXUVVdCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KFOUQjDt[ZRpd2RuIFnDOVA:OTJibl2= M{XRVlE6PTRzNEiz
human A375 cells MXnDfZRwfG:6aXRCpIF{e2G7 MUW3NkBp MmLVR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVM4PSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;MUOgcm0> NYXQR2M{OjF|NEG2O|Q>
COR-L23/P cell NFT5PHFEgXSxdH;4bYPDqGG|c3H5 MV7DfZRwfG:6aXPpeJkhdWWjc4Xy[YQhfXOrbnegeIhmKEORUj3MNlMheGG{ZX70ZYwhMEORUj3MNlMwWCliaIXtZY4hdm:wIIPtZYxtKGOnbHygcJVv\yClYYLjbY5wdWFiY3XscEBtcW6nLDDJR|UxRTF|LkKgcm0> NFjV[I8yOThyNkeyOC=>
human NCI-H460 cells NUS5XoZYWHKxbHnm[ZJifGmxbjDhd5NigQ>? MWW3NkBp MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc5VtfGW{IHPveY51\XJiYX7hcJl{cXNuIFnDOVA:OTZibl2= MnTWNlE4QDN|Nkm=
human MOLT4 cells MnK1R5l1d3SxeHnjxsBie3OjeR?= MoTDO|IhcA>? MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG5JI5O MUGyOFkxODd{NR?=
human LoVo cells MlOwR5l1d3SxeHnjxsBie3OjeR?= M3TJNFczKGh? MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkCgcm0> MXeyNFM4OTF6Mx?=
RPM18402 tumor cell line NVq3TWdFWHKxbHnm[ZJifGmxbjDhd5NigQ>? MoruR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IHPlcIwheHKxbHnm[ZJifGmxbjDpckBTWE1zOESwNkB1fW2xcjDj[YxtKGyrbnWsJGlEPTB;MkCgcm0> NX7mRlI4OTV2OEK5Nlk>
human SN12C cells M3G5Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXjHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTUlEzSyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEeLNUC9NlAhdk1? MWKyOVkxQTJ5OR?=
human RPMI8226 cells NU\lR48{S3m2b4TvfIlkyqCjc4PhfS=> MVjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSVG1KQDJ{NjDj[YxteyCkeTDNWHQhdWW2aH;kMEBKSzVyPUKxJI5O NVfH[Il{OTlyMUK5PVY>
HT-29 cells NIX4TVJEgXSxdH;4bYPDqGG|c3H5 NVzvS4RoS3m2b4TvfIlkcXS7IIfhd{Bl\XSncn3pcoVlKGmwII\peJJwKGmwIFjUMVI6KGOnbHzzLINwdG:wKTDv[kBpfW2jbjD0eY1weiClZXzsJIxqdmW|IHL5JJV{cW6pIF3UWEBie3OjeTygTWM2OD1{NTDuUS=> MV23PFU{OzNz
SK-BR-3 cells MnjXR5l1d3SxeHnjxsBie3OjeR?= MnznTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmViKGPLMWJTNTNrLDDJR|UxRTJ4IH7N NGCzeHMyOTN|NEW2PS=>
HeLa cells M{exbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWXoO3ZHT3Kxd4ToJIlvcGmkaYTpc44hd2ZiSHXMZUBk\WyuczDh[pRmeiB2IHThfZMtKEeLNUC9N|Ahdk1? M3PBflE4PDF6NUiy
UACC 62 cells NGTCfXZEgXSxdH;4bYPDqGG|c3H5 NFzpOJRV\XO2ZXSgbY4hfmm2cn:g[o9zKGO7dH;0c5hq[2m2eTDh[4FqdnO2IHj1cYFvKHS3bX;yJINmdGxibHnu[UBWSUOFIE[yJEhu\Wyjbn;tZUktKEeLNUC9N|Ahdk1? NGftb|IyODdzNEWwNi=>
human HCT116 cells NGTQSHpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXTPeWI3T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IHL5JIZqfmVvZH;z[UBoem:5dHigbY5pcWKrdHnvckBie3OjeTygS2k2OD1|MDDuUS=> MWGyOVkxQTJ5OR?=
human lung cancer cell line (H128) NEHnbJpEgXSxdH;4bYPDqGG|c3H5 M3LJXGlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx2dmdiY3HuZ4VzKGOnbHygcIlv\SBqSEGyPEktKEmFNUC9N|Ehdk1? MX[xNVM{PDV4OR?=
human MES-SA/Dx5 cells NYPHemo5S3m2b4TvfIlkyqCjc4PhfS=> MlTMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWVUNVODL1T4OUBk\WyuczDveoVz\XiycnXzd4lv\yCPRGKxJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD1|MzDuUS=> M1P1OlIyOzRzNke0
human A2780 cells MYjDfZRwfG:6aXRCpIF{e2G7 M{nYUlczKGh? M{L5Z2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9N|Mhdk1? M{TyOVI1QTByN{K1
human MDA-MB-435 cells M3jU[2N6fG:2b4jpZ:Kh[XO|YYm= MoXCO|IhcA>? NVjo[m5kS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGdKPTB;M{Sgcm0> MlXKNlI5PjdyMUm=
human stomach cancer cell line (MKN45) NIDON3REgXSxdH;4bYPDqGG|c3H5 MlL1TY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gd5RwdWGlaDDjZY5k\XJiY3XscEBtcW6nIDjNT241PSluIFnDOVA:Ozhibl2= MmXmNVE{OzR3Nkm=
human KB3-1 cells MX\DfZRwfG:6aXRCpIF{e2G7 Mmi1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2I{NTFiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUSwJI5O MnTXNVg5Ojl|M{S=
mouse P388 cells MYHDfZRwfG:6aXRCpIF{e2G7 MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDQN|g5KGOnbHzzJIFnfGW{IF3UWEBie3OjeTygTWM2OD12NTDuUS=> M1\vS|E5QDJ7M{O0
A427 human lung carcinoma M4j3VnBzd2yrZnXyZZRqd25iYYPzZZk> M3riWGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JI1m[XO3cnXkJIFo[Wmwc4SgRVQzPyCqdX3hckBtfW6pIHPhdoNqdm:vYTygTWM2OD12OTDuUS=> NVrkT3YxQTh5NkGxNS=>
human U251 cells NIXaXW1HfW6ldHnvckBie3OjeR?= MkLYOk0zPCCq MYDJcohq[mm2aX;uJI9nKHSxcH;pd49u\XKjc3WtNUBqdiCqdX3hckBWOjVzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcHmyb4jpZU1qdmS3Y3XkJGhKTi1zYXzwbIEh[WOldX31cIF1cW:wIHnuJI52[2ynYYKg[Zh1emGldDDh[pRmeiB4IITvJFI1KGi{czDifUBqdW23bn;icI91KGGwYXz5d4l{NCCHQ{WwQVU1KG6P MnPTNlI{ODV4MUK=
human colon cancer cell line (WiDr) NFr6Wm1EgXSxdH;4bYPDqGG|c3H5 M3z4OmlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJINwdG:wIHPhcoNmeiClZXzsJIxqdmViKGfpSJIqNCCLQ{WwQVU3KG6P NEfM[3YyOTN|NEW2PS=>
human HepG2 cells MnnLR5l1d3SxeHnjxsBie3OjeR?= MkfxOEBl[Xm| M1;1cmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9OlAhdk1? NH\B[2cyQDV3NEmwOi=>
human KB cells NV7q[GdRS3m2b4TvfIlkyqCjc4PhfS=> NFjhZXg4OiCq M{LVfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IEeyJIhzeyC|dXzmc5Jpd2SjbXnu[UBDKGOxbH;ybY1mfHKrYzDhd5NigSxiSVO1NF03Oi53IH7N NFXCd2UzPTByOES1Oi=>
human HCT8 cells NVm1PXBES3m2b4TvfIlkyqCjc4PhfS=> MnTMN{Bl[Xm| NEn0b5dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1R6IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9O|Ahdk1? NYnlPZpWOjB|OUK1OFU>
human GBM2 cells NISwWppRem:uaX\ldoF1cW:wIHHzd4F6 NWXLT2RGPzJiaB?= NUfveJkySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0zKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:OC5zMzFOwG0> NYDkfZBROjZ|NUW1N|I>
SK-MEL-2 cells NUG4eHliS3m2b4TvfIlkyqCjc4PhfS=> MV23NkBp NH3KN5ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2PRVytNkBk\WyuczDh[pRmeiB5MjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVA:OC5zNDFOwG0> NI\qTYkzOTN2MU[3OC=>
human IGROV1 cells MorLR5l1d3SxeHnjxsBie3OjeR?= MkfjO|IhcA>? MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDJS3JQXjFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUCuNVYh|ryP M{PkSVIyOzRzNke0
human U87 cells Mm\vVJJwdGmoZYLheIlwdiCjc4PhfS=> NYi3WXBTPDhiaB?= MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMU[g{txO NGW5WHczPDh{NkixPC=>
human HT1080 cells NFLoZoVRem:uaX\ldoF1cW:wIHHzd4F6 NVzXRXBoPDhiaB?= NFHhVWZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUNVA5OCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDJR|UxRTBwMUig{txO NGq0co0zPjRyOEixOS=>
human Hep3B cells M4jabnBzd2yrZnXyZZRqd25iYYPzZZk> NFPa[HM4OiCq NV64V5hYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZA{SiClZXzsd{Bi\nSncjC3NkBpenNiYomgXHRVKGG|c3H5MEBIUTVyPUCuNlIh|ryP MVSxPVc6Pjl3Nh?=
human GBM3 cells NF\oc5BRem:uaX\ldoF1cW:wIHHzd4F6 M33OT|czKGh? NXvkXlB4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0{KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:OC5|ODFOwG0> M{XoblI3OzV3NUOy
human KBVIN cells Mni1R5l1d3SxeHnjxsBie3OjeR?= NWnLT5d2PzJiaB?= MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRnZKViClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUCuOEDPxE1? MXSyOVQ{QDd4OR?=
human GBM1 cells NFPNeWVRem:uaX\ldoF1cW:wIHHzd4F6 MXq3NkBp MnnxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCJQl2xJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDXV3QuOSCvZYToc4QtKEmFNUC9NE41PiEQvF2= M3;2WFI3OzV3NUOy
human U937 cells NXqyRmJRS3m2b4TvfIlkyqCjc4PhfS=> MXi0PEBp MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIV5eHKnc4PpcocheDV|IH31eIFvfCCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6KO,:jDDJR|UxRTFwMTFOwG0> NFPtRlMyQTdzNUOxPS=>

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
in solvent
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID