Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells MVXDfZRwfG:6aXRCpIF{e2G7 NIrPOnk4OiCq NGq3OG5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJIlkPTB;MTDuUS=> M4HROVIxOzdzMUiz
human Bel7402 cells NGrSWYJEgXSxdH;4bYPDqGG|c3H5 NH7jRXk6PiCq NGjzSXFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NECyJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS5{IH7N M{W3SFE2OTZ3MUS0
human HCT8 cells M1G3TmN6fG:2b4jpZ:Kh[XO|YYm= MnXwPVYhcA>? MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3Q5KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT61JI5O NIjKeGgyPTF4NUG0OC=>
human MCF7 cells M3SzfGN6fG:2b4jpZ:Kh[XO|YYm= MU[5OkBp M3rJbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xMlghdk1? Mm[4NVUyPjVzNES=
human A549 cells M2PZcGN6fG:2b4jpZ:Kh[XO|YYm= MV65OkBp NWj3eFNtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVMvOiCwTR?= M2PhUVE2OTZ3MUS0
human colon carcinoma SW620 cell MWTDfZRwfG:6aXRCpIF{e2G7 MonUR5l1d3SxeHnjJJBwfGWwdHnheIlwdiCxZjDUc5BwfGWlYX6gLHRRMSCkeTD0bIUh[2:vcH;1coQhcW5iaIXtZY4h[2:ub36gZ4Fz[2mwb33hJHNYPjJyIHPlcIwhdGmwZTygTWM2OD1|LkKgcm0> NFrz[pQyOjRyOEewOy=>
human MESSA cells MYnDfZRwfG:6aXRCpIF{e2G7 NVT3SYM6PzJiaB?= NEWxbnhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVOVQTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9OEBvVQ>? M2HzRVIyOzRzNke0
human BGC823 cells MofIR5l1d3SxeHnjxsBie3OjeR?= NXO4fFJ6QTZiaB?= Ml3OR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ|IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE4{KG6P NIfnbXIyPTF4NUG0OC=>
human Ketr3 cells MmTLR5l1d3SxeHnjxsBie3OjeR?= M4q3Slk3KGh? MoT6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT4V1ejNiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20Mlkhdk1? MljlNVUyPjVzNES=
LOX cells NU\nO4xjS3m2b4TvfIlkyqCjc4PhfS=> NUfiVZhZS3m2b4TvfIlkcXS7IIfhd{Bl\XSncn3pcoVlKGmwII\peJJwKGmwIFzPXEBk\WyuczCocYVt[W6xbXGpJI9nKGi3bXHuJJR2dW:{IHPlcIwhdGmwZYOgZpkhfXOrbnegUXRVKGG|c3H5MEBKSzVyPUWgcm0> NUTiToxkPzh3M{OzNS=>
human A549 cells Mo\qSpVv[3Srb36gZZN{[Xl? MoHwOEBp NGHxSopCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IESgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD13IH7N MnLVNVgzODd5NEi=
human H69 cells M3HJZ2N6fG:2b4jpZ:Kh[XO|YYm= MnexO|IhcA>? M2LzPWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGg3QSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;NjDuUS=> NVXzVI55OjF|NEG2O|Q>
human A2780 cells NETBWplEgXSxdH;4bYPDqGG|c3H5 NX;IdFY6PzJiaB?= NVe1cXBWS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNib4\ldoV5eHKnc4Ppcoch[WyyaHG1ZoV1[TNiaX70[YdzcW5iYYPz[ZN{\WRiYYOgZ4VtdCC|dYL2bZZidCCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVYhdk1? MX[yNlk2QTJ2Nh?=
human LNCAP cells NGjyb5pEgXSxdH;4bYPDqGG|c3H5 M{LxbFczKGh? NHjTXpdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVkODUDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9PUBvVQ>? NGHsdHUzOTN2MU[3OC=>
human HOP62 cells M4\jb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{nudWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhQWDZ{IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= MWeyOVkxQTJ5OR?=
human MCF7 cells NH2yTGZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml\JS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWNHPyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEmFNUC9NVAhdk1? NUS2e5pzOjV7MEmyO|k>
human UACC62 cells NUmxRoxQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3TvSGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHVCS0N4MjDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= MYmyOVkxQTJ5OR?=
human DU145 cells M1[yU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH3ONY9Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= MXGyOVkxQTJ5OR?=
LoVo cell line NIXoRodEgXSxdH;4bYPDqGG|c3H5 MlXFR5l1d3SxeHnjJJBwfGWwdHnheIlwdiCxZjDUc5BwfGWlYX6gLHRRMSCkeTD0bIUh[2:vcH;1coQhcW5iKHj1cYFvKGOxbH;y[YN1[WxiY3HuZ4VzKEyxVn:gZ4VtdCCuaX7lMEBKSzVyPUGxMlYhdk1? M1W0NVEzPDB6N{C3
human lymphoblast tumor cell line RPM18402 NInXT|ZEgXSxdH;4bYPDqGG|c3H5 MmnSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gcJlueGixYnzhd5QhfHWvb4KgZ4VtdCCuaX7lJHJRVTF6NECyMEBKSzVyPUGyJI5O M4D5[VEzOzl{N{S1
human HL60 cells M1XkcGZ2dmO2aX;uJIF{e2G7 NWqydYVqSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCVUlKgcYV1cG:mLDDJR|UxRTF{IH7N MmPoNVk2PDF2OEO=
human A375 cells NEDoTmxEgXSxdH;4bYPDqGG|c3H5 M4TIb|czKGh? MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD1zMzDuUS=> MVSyNVM1OTZ5NB?=
COR-L23/P cell MXrDfZRwfG:6aXRCpIF{e2G7 M{nMcmN6fG:2b4jpZ4l1gSCvZXHzeZJm\CC3c3nu[{B1cGViQ1;SMWwzOyCyYYLlcpRidCBqQ1;SMWwzOy:SKTDoeY1idiCwb36gd41idGxiY3XscEBtfW6pIHPhdoNqdm:vYTDj[YxtKGyrbnWsJGlEPTB;MUOuNkBvVQ>? NXTYdnF1OTF6ME[3NlQ>
human NCI-H460 cells NEG0e|JRem:uaX\ldoF1cW:wIHHzd4F6 MV[3NkBp MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc5VtfGW{IHPveY51\XJiYX7hcJl{cXNuIFnDOVA:OTZibl2= NIiydmwzOTd6M{O2PS=>
human MOLT4 cells MUfDfZRwfG:6aXRCpIF{e2G7 MV[3NkBp MnzYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUW9NXDRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPUBvVQ>? MoLUNlQ6ODB5MkW=
human LoVo cells NEXlU21EgXSxdH;4bYPDqGG|c3H5 NFewSXg4OiCq MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkCgcm0> MWeyNFM4OTF6Mx?=
RPM18402 tumor cell line NE\uTY1Rem:uaX\ldoF1cW:wIHHzd4F6 NVTvelNOS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JINmdGxicILvcIln\XKjdHnvckBqdiCUUF2xPFQxOiC2dX3vdkBk\WyuIHzpcoUtKEmFNUC9NlAhdk1? NVjDWFVtOTV2OEK5Nlk>
human SN12C cells MmXJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH7ReI5Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUVjF{QzDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFfJOVA:OjBibl2= NFrmfJEzPTlyOUK3PS=>
human RPMI8226 cells MlfYR5l1d3SxeHnjxsBie3OjeR?= Mo[4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVnBOUTh{Mk[gZ4VtdHNiYomgUXRVKG2ndHjv[EwhUUN3ME2yNUBvVQ>? NVjKU|dQOTlyMUK5PVY>
HT-29 cells NWT6UZQ2S3m2b4TvfIlkyqCjc4PhfS=> MYfDfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hUFRvMkmgZ4VtdHNqY3;sc44qKG:oIHj1cYFvKHS3bX;yJINmdGxibHnu[ZMh[nlidYPpcochVVSWIHHzd4F6NCCLQ{WwQVI2KG6P MVe3PFU{OzNz
SK-BR-3 cells MofhR5l1d3SxeHnjxsBie3OjeR?= NH3UUHVKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZTCoV2suSlJvMzmsJGlEPTB;Mk[gcm0> Ml2zNVE{OzR3Nkm=
HeLa cells M1fMOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M160cWdzd3e2aDDpcohq[mm2aX;uJI9nKEinTHGgZ4VtdHNiYX\0[ZIhPCCmYYnzMEBIUTVyPUOwJI5O M2DidVE4PDF6NUiy
UACC 62 cells Mn\wR5l1d3SxeHnjxsBie3OjeR?= NUDiWlVtXGW|dHXkJIlvKH[rdILvJIZweiCleYTveI95cWOrdImgZYdicW6|dDDoeY1idiC2dX3vdkBk\WyuIHzpcoUhXUGFQzC2NkApdWWuYX7vcYEqNCCJSUWwQVMxKG6P MlPLNVA4OTR3MEK=
human HCT116 cells NUjFeIlNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCJSUWwQVMxKG6P NIrCU4YzPTlyOUK3PS=>
human lung cancer cell line (H128) MlHRR5l1d3SxeHnjxsBie3OjeR?= NWPLS|RQUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdHWwZzDjZY5k\XJiY3XscEBtcW6nIDjINVI5MSxiSVO1NF0{OSCwTR?= NXzWVHA2OTF|M{S1Olk>
human MES-SA/Dx5 cells MnvkR5l1d3SxeHnjxsBie3OjeR?= NITJd|ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVNvU1GvSJg2KGOnbHzzJI93\XKneIDy[ZN{cW6pIF3EVlEh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUOzJI5O MY[yNVM1OTZ5NB?=
human A2780 cells MoXjR5l1d3SxeHnjxsBie3OjeR?= NXHJNHRtPzJiaB?= NYfEdGxKS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0{OyCwTR?= NISzSIEzPDlyMEeyOS=>
human MDA-MB-435 cells NEDCWJVEgXSxdH;4bYPDqGG|c3H5 MVm3NkBp NIrZZXFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2KGOnbHzzJIlv[3WkYYTl[EBnd3JiN{KgbJJ{KGK7IF3UWEBie3OjeTygS2k2OD1|NDDuUS=> MXGyNlg3PzBzOR?=
human stomach cancer cell line (MKN45) MkDKR5l1d3SxeHnjxsBie3OjeR?= MmDWTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gd5RwdWGlaDDjZY5k\XJiY3XscEBtcW6nIDjNT241PSluIFnDOVA:Ozhibl2= NGjIV5oyOTN|NEW2PS=>
human KB3-1 cells NEDYTllEgXSxdH;4bYPDqGG|c3H5 M4ToUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDOy1zIHPlcIx{KGGodHXyJG1VXCCjc4PhfUwhUUN3ME20NEBvVQ>? MmHENVg5Ojl|M{S=
mouse P388 cells MYHDfZRwfG:6aXRCpIF{e2G7 NFixbZNEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBROzh6IHPlcIx{KGGodHXyJG1VXCCjc4PhfUwhUUN3ME20OUBvVQ>? M37tdVE5QDJ7M{O0
A427 human lung carcinoma NV7YUVQ2WHKxbHnm[ZJifGmxbjDhd5NigQ>? NF7lVoJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBu\WG|dYLl[EBi\2GrboP0JGE1OjdiaIXtZY4hdHWwZzDjZZJkcW6xbXGsJGlEPTB;NEmgcm0> MlHtPVg4PjFzMR?=
human U251 cells NF7YZWhHfW6ldHnvckBie3OjeR?= MmnpOk0zPCCq NHTReXRKdmirYnn0bY9vKG:oIITvdI9qe2:vZYLhd4UuOSCrbjDoeY1idiCXMkWxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiaInwc5hq[S2rbnT1Z4VlKEiLRj2xZYxxcGFiYXPjeY12dGG2aX;uJIlvKG63Y3zlZZIh\Xi2cnHjeEBi\nSncjC2JJRwKDJ2IHjyd{BjgSCrbX31co9jdG:2IHHuZYx6e2m|LDDFR|UxRTV2IH7N MlzJNlI{ODV4MUK=
human colon cancer cell line (WiDr) MVPDfZRwfG:6aXRCpIF{e2G7 M{O3ZmlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJINwdG:wIHPhcoNmeiClZXzsJIxqdmViKGfpSJIqNCCLQ{WwQVU3KG6P MXWxNVM{PDV4OR?=
human HepG2 cells MnPCR5l1d3SxeHnjxsBie3OjeR?= MXq0JIRigXN? NUD1bYozS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KE2WVDDhd5NigSxiSVO1NF03OCCwTR?= MlTnNVg2PTR7ME[=
human KB cells NGLHV5JEgXSxdH;4bYPDqGG|c3H5 M4HFbFczKGh? MlW3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KHO3bH\vdohw\GGvaX7lJGIh[2:ub4LpcYV1emmlIHHzd4F6NCCLQ{WwQVYzNjVibl2= M3HvWlI2ODB6NEW2
human HCT8 cells M{fOSWN6fG:2b4jpZ:Kh[XO|YYm= NX;5dXB1OyCmYYnz MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3Q5KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;N{Cgcm0> M1rndFIxOzl{NUS1
human GBM2 cells MYnQdo9tcW[ncnH0bY9vKGG|c3H5 NF3U[HU4OiCq M1PBTGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOTNizszN Mk\vNlY{PTV3M{K=
SK-MEL-2 cells NF3ifYNEgXSxdH;4bYPDqGG|c3H5 NULMVpFYPzJiaB?= MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMjDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NE4yPCEQvF2= MVOyNVM1OTZ5NB?=
human IGROV1 cells MX;DfZRwfG:6aXRCpIF{e2G7 NH\QOXM4OiCq NFXWNYZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBKT1KRVkGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvOTZizszN NVzUV2FTOjF|NEG2O|Q>
human U87 cells MW\Qdo9tcW[ncnH0bY9vKGG|c3H5 Ml62OFghcA>? NY\5OlJ5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFch[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlE3KM7:TR?= MYKyOFgzPjhzOB?=
human HT1080 cells MWnQdo9tcW[ncnH0bY9vKGG|c3H5 Ml3DOFghcA>? MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwQVAvOThizszN NYPKeGZ[OjZ2MEi4NVU>
human Hep3B cells MkXHVJJwdGmoZYLheIlwdiCjc4PhfS=> MnnYO|IhcA>? MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincEPCJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC[VGSgZZN{[XluIFfJOVA:OC5{MjFOwG0> Mn[0NVk4QTZ7NU[=
human GBM3 cells Mnq2VJJwdGmoZYLheIlwdiCjc4PhfS=> MlvjO|IhcA>? MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEeETUOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiN{KgbJJ{KGK7IGfTWE0yKG2ndHjv[EwhUUN3ME2wMlM5KM7:TR?= Mn\XNlY{PTV3M{K=
human KBVIN cells Mn;QR5l1d3SxeHnjxsBie3OjeR?= NVSzNldTPzJiaB?= MmPkR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2JXUU5iY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlQh|ryP M2C4Z|I2PDN6N{[5
human GBM1 cells MYDQdo9tcW[ncnH0bY9vKGG|c3H5 NFHBR5E4OiCq M2DKPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvPDZizszN NXrGPIlKOjZ|NUW1N|I>
human U937 cells MmLmR5l1d3SxeHnjxsBie3OjeR?= MoDXOFghcA>? Mn61R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{BmgHC{ZYPzbY5oKHB3MzDteZRidnRiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSExvJygTWM2OD1zLkGg{txO NYPPfllqOTl5MUWzNVk>

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents individually and in order:
Saline
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID