Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NFXSfnJEgXSxdH;4bYPDqGG|c3H5 MWm3NkBp NIKy[XlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJIlkPTB;MTDuUS=> M2K5dVIxOzdzMUiz
human Bel7402 cells MYjDfZRwfG:6aXRCpIF{e2G7 M3T5UFk3KGh? MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU4zKG6P M2X4dlE2OTZ3MUS0
human HCT8 cells M2rvcGN6fG:2b4jpZ:Kh[XO|YYm= MYG5OkBp MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3Q5KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT61JI5O NVm4XpBwOTVzNkWxOFQ>
human MCF7 cells M4\TOGN6fG:2b4jpZ:Kh[XO|YYm= NW\sV293QTZiaB?= M3LlVmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xMlghdk1? NF3aW4wyPTF4NUG0OC=>
human A549 cells NEnUfHlEgXSxdH;4bYPDqGG|c3H5 M1jROlk3KGh? MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mz6yJI5O NV7sbIlHOTVzNkWxOFQ>
human colon carcinoma SW620 cell MWPDfZRwfG:6aXRCpIF{e2G7 M4jJfmN6fG:2b4jpZ{Bxd3SnboTpZZRqd25ib3[gWI9xd3SnY3HuJEhVWCliYomgeIhmKGOxbYDveY5lKGmwIHj1cYFvKGOxbH;uJINiemOrbn;tZUBUXzZ{MDDj[YxtKGyrbnWsJGlEPTB;Mz6yJI5O NYDWNXI1OTJ2MEi3NFc>
human MESSA cells M2rMRmN6fG:2b4jpZ:Kh[XO|YYm= Mom5O|IhcA>? Mne3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWVUW0FiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUSgcm0> MkDjNlE{PDF4N{S=
human BGC823 cells NFvtSmFEgXSxdH;4bYPDqGG|c3H5 NIPuR|E6PiCq MoTSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ|IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE4{KG6P NXziemF4OTVzNkWxOFQ>
human Ketr3 cells NHvKeVhEgXSxdH;4bYPDqGG|c3H5 MoKzPVYhcA>? NGHaUXVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBM\XS{MzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQvQSCwTR?= NHrqW4MyPTF4NUG0OC=>
LOX cells MlXzR5l1d3SxeHnjxsBie3OjeR?= NVLvXpdTS3m2b4TvfIlkcXS7IIfhd{Bl\XSncn3pcoVlKGmwII\peJJwKGmwIFzPXEBk\WyuczCocYVt[W6xbXGpJI9nKGi3bXHuJJR2dW:{IHPlcIwhdGmwZYOgZpkhfXOrbnegUXRVKGG|c3H5MEBKSzVyPUWgcm0> NUXZdGVQPzh3M{OzNS=>
human A549 cells MmrnSpVv[3Srb36gZZN{[Xl? NHHzSYs1KGh? NULWeYF1SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NTDuUS=> NHfM[pAyQDJyN{e0PC=>
human H69 cells NXTp[nA{S3m2b4TvfIlkyqCjc4PhfS=> NF7xVVc4OiCq NEXhOVBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPjliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[gcm0> NULxblY3OjF|NEG2O|Q>
human A2780 cells MXvDfZRwfG:6aXRCpIF{e2G7 MYS3NkBp NX7iZXpMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNib4\ldoV5eHKnc4Ppcoch[WyyaHG1ZoV1[TNiaX70[YdzcW5iYYPz[ZN{\WRiYYOgZ4VtdCC|dYL2bZZidCCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVYhdk1? NET4SoUzOjl3OUK0Oi=>
human LNCAP cells NFO4NVdEgXSxdH;4bYPDqGG|c3H5 NFO1V3E4OiCq NFfvPHlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVkODUDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9PUBvVQ>? MnLJNlE{PDF4N{S=
human HOP62 cells MnnDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIU3A3OiClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEmFNUC9NVAhdk1? MlfjNlU6ODl{N{m=
human MCF7 cells M{nWUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmDCS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWNHPyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEmFNUC9NVAhdk1? NH:yWWczPTlyOUK3PS=>
human UACC62 cells MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1HZUWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHVCS0N4MjDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= NIfZXpEzPTlyOUK3PS=>
human DU145 cells MoPYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NY\qbHJpT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hTFVzNEWgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N M1W2WFI2QTB7Mke5
LoVo cell line MX3DfZRwfG:6aXRCpIF{e2G7 NF63OoVEgXSxdH;4bYMheG:2ZX70bYF1cW:wIH;mJHRweG:2ZXPhckApXFBrIHL5JJRp\SClb33wc5Vv\CCrbjCobJVu[W5iY3;sc5Jm[3SjbDDjZY5k\XJiTH;Wc{Bk\WyuIHzpcoUtKEmFNUC9NVEvPiCwTR?= Ml;ONVI1ODh5MEe=
human lymphoblast tumor cell line RPM18402 M3[2WmN6fG:2b4jpZ:Kh[XO|YYm= MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDsfY1xcG:kbHHzeEB1fW2xcjDj[YxtKGyrbnWgVnBOOTh2MEKsJGlEPTB;MUKgcm0> M1L3PFEzOzl{N{S1
human HL60 cells M2LEZ2Z2dmO2aX;uJIF{e2G7 NWe2O|V5SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCVUlKgcYV1cG:mLDDJR|UxRTF{IH7N MYKxPVU1OTR6Mx?=
human A375 cells NVvz[mU2S3m2b4TvfIlkyqCjc4PhfS=> NIfsNXE4OiCq M1rRd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUGzJI5O NHHBXlUzOTN2MU[3OC=>
COR-L23/P cell NUWxbJZmS3m2b4TvfIlkyqCjc4PhfS=> MoDxR5l1d3SxeHnjbZR6KG2nYYP1doVlKHW|aX7nJJRp\SCFT2KtUFI{KHCjcnXueIFtKCiFT2KtUFI{N1BrIHj1cYFvKG6xbjDzcYFtdCClZXzsJIx2dmdiY3HyZ4lvd22jIHPlcIwhdGmwZTygTWM2OD1zMz6yJI5O M{P6dFEyQDB4N{K0
human NCI-H460 cells MVTQdo9tcW[ncnH0bY9vKGG|c3H5 MkHIO|IhcA>? NXPMSXV4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2:3bITldkBkd3WwdHXyJIFv[Wy7c3nzMEBKSzVyPUG2JI5O NELxfYQzOTd6M{O2PS=>
human MOLT4 cells NXXjfJhXS3m2b4TvfIlkyqCjc4PhfS=> MUK3NkBp MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG5JI5O MWSyOFkxODd{NR?=
human LoVo cells Mn;UR5l1d3SxeHnjxsBie3OjeR?= NFfmXZk4OiCq MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkCgcm0> NVjLO2UxOjB|N{GxPFM>
RPM18402 tumor cell line NHvjW3hRem:uaX\ldoF1cW:wIHHzd4F6 MnvjR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IHPlcIwheHKxbHnm[ZJifGmxbjDpckBTWE1zOESwNkB1fW2xcjDj[YxtKGyrbnWsJGlEPTB;MkCgcm0> NF3K[40yPTR6MkmyPS=>
human SN12C cells M17DNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTUlEzSyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEeLNUC9NlAhdk1? M3r6d|I2QTB7Mke5
human RPMI8226 cells MV\DfZRwfG:6aXRCpIF{e2G7 NH7uOJFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTWE2LOEKyOkBk\WyuczDifUBOXFRibXX0bI9lNCCLQ{WwQVIyKG6P MXqxPVAyOjl7Nh?=
HT-29 cells NVvqTY5[S3m2b4TvfIlkyqCjc4PhfS=> MYjDfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hUFRvMkmgZ4VtdHNqY3;sc44qKG:oIHj1cYFvKHS3bX;yJINmdGxibHnu[ZMh[nlidYPpcochVVSWIHHzd4F6NCCLQ{WwQVI2KG6P MVK3PFU{OzNz
SK-BR-3 cells NH7oO21EgXSxdH;4bYPDqGG|c3H5 MojPTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmViKGPLMWJTNTNrLDDJR|UxRTJ4IH7N M{PGO|EyOzN2NU[5
HeLa cells NVG3fVI6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3KzTmdzd3e2aDDpcohq[mm2aX;uJI9nKEinTHGgZ4VtdHNiYX\0[ZIhPCCmYYnzMEBIUTVyPUOwJI5O MmPyNVc1OTh3OEK=
UACC 62 cells MojaR5l1d3SxeHnjxsBie3OjeR?= MojuWIV{fGWmIHnuJJZqfHKxIH\vdkBkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB1fW2xcjDj[YxtKGyrbnWgWWFESyB4MjCocYVt[W6xbXGpMEBIUTVyPUOwJI5O MVSxNFcyPDVyMh?=
human HCT116 cells MkPUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYPHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCJSUWwQVMxKG6P M4e4W|I2QTB7Mke5
human lung cancer cell line (H128) MlrJR5l1d3SxeHnjxsBie3OjeR?= NFnSPZlKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBtfW6pIHPhcoNmeiClZXzsJIxqdmViKFixNlgqNCCLQ{WwQVMyKG6P M{naRlEyOzN2NU[5
human MES-SA/Dx5 cells M{\VSmN6fG:2b4jpZ:Kh[XO|YYm= M4\tU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1GWy2VQT;EfFUh[2WubIOgc5ZmemW6cILld5NqdmdiTVTSNUBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;M{Ogcm0> M4LtU|IyOzRzNke0
human A2780 cells MYfDfZRwfG:6aXRCpIF{e2G7 MVW3NkBp NEL3SGxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM{KG6P M4fKOFI1QTByN{K1
human MDA-MB-435 cells M2LxOmN6fG:2b4jpZ:Kh[XO|YYm= NV\jTpdbPzJiaB?= NYXYU4NOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGdKPTB;M{Sgcm0> MV[yNlg3PzBzOR?=
human stomach cancer cell line (MKN45) MUDDfZRwfG:6aXRCpIF{e2G7 MWTJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDzeI9u[WOqIHPhcoNmeiClZXzsJIxqdmViKF3LUlQ2MSxiSVO1NF0{QCCwTR?= NITX[m0yOTN|NEW2PS=>
human KB3-1 cells NFHKcJpEgXSxdH;4bYPDqGG|c3H5 MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRlMuOSClZXzsd{Bi\nSncjDNWHQh[XO|YYmsJGlEPTB;NECgcm0> NF20OGcyQDh{OUOzOC=>
mouse P388 cells NH;UXY9EgXSxdH;4bYPDqGG|c3H5 MorLR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgVFM5QCClZXzsd{Bi\nSncjDNWHQh[XO|YYmsJGlEPTB;NEWgcm0> M2n1PFE5QDJ7M{O0
A427 human lung carcinoma MYXQdo9tcW[ncnH0bY9vKGG|c3H5 M4mxV2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JI1m[XO3cnXkJIFo[Wmwc4SgRVQzPyCqdX3hckBtfW6pIHPhdoNqdm:vYTygTWM2OD12OTDuUS=> NIDNVII6QDd4MUGx
human U251 cells MYrGeY5kfGmxbjDhd5NigQ>? NEXwZVQ3NTJ2IHi= Mon5TY5pcWKrdHnvckBw\iC2b4DvbZNwdWW{YYPlMVEhcW5iaIXtZY4hXTJ3MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBJUUZvMXHsdIhiKGGlY4XteYxifGmxbjDpckBvfWOuZXHyJIV5fHKjY4SgZYZ1\XJiNjD0c{AzPCCqcoOgZpkhcW2vdX7vZoxwfCCjbnHsfZNqeyxiRVO1NF02PCCwTR?= NHuyeXQzOjNyNU[xNi=>
human colon cancer cell line (WiDr) NGHsXGdEgXSxdH;4bYPDqGG|c3H5 NXTjOoFHUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIwhdGmwZTCoW4lFeiluIFnDOVA:PTZibl2= MoD2NVE{OzR3Nkm=
human HepG2 cells NGH0VmJEgXSxdH;4bYPDqGG|c3H5 MmjUOEBl[Xm| MmjsR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOEBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME22NEBvVQ>? M{nUb|E5PTV2OUC2
human KB cells MlPvR5l1d3SxeHnjxsBie3OjeR?= NYPCSGFFPzJiaB?= MV;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMhe3WuZn;ybI9l[W2rbnWgRkBkd2yxcnnt[ZRzcWNiYYPzZZktKEmFNUC9OlIvPSCwTR?= NI\VZoQzPTByOES1Oi=>
human HCT8 cells NYm0NGt4S3m2b4TvfIlkyqCjc4PhfS=> NXjJd5FjOyCmYYnz NXe3SVFuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVcxKG6P MYmyNFM6OjV2NR?=
human GBM2 cells NIrxUlZRem:uaX\ldoF1cW:wIHHzd4F6 MWS3NkBp M4fOemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOTNizszN NWnhbIxnOjZ|NUW1N|I>
SK-MEL-2 cells MnT2R5l1d3SxeHnjxsBie3OjeR?= NXyyeVNrPzJiaB?= MmnoR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOLUKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvOTRizszN MYGyNVM1OTZ5NB?=
human IGROV1 cells M{XIfGN6fG:2b4jpZ:Kh[XO|YYm= NUO0WmhwPzJiaB?= M1HsWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGlIWk:YMTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NE4yPiEQvF2= NHPwZ2czOTN2MU[3OC=>
human U87 cells MmjjVJJwdGmoZYLheIlwdiCjc4PhfS=> NG\BUoI1QCCq MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMU[g{txO MViyOFgzPjhzOB?=
human HT1080 cells NYXjdJo1WHKxbHnm[ZJifGmxbjDhd5NigQ>? NUT5bppTPDhiaB?= Mm\rRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEGwPFAh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44hcW6ldXLheIVlKG[xcjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NF0xNjF6IN88US=> NGnHVYkzPjRyOEixOS=>
human Hep3B cells NYPKTXk{WHKxbHnm[ZJifGmxbjDhd5NigQ>? M3nmeFczKGh? NVrEOnZMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZA{SiClZXzsd{Bi\nSncjC3NkBpenNiYomgXHRVKGG|c3H5MEBIUTVyPUCuNlIh|ryP M3Hz[FE6Pzl4OUW2
human GBM3 cells NUfUXnE3WHKxbHnm[ZJifGmxbjDhd5NigQ>? MV:3NkBp MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEeETUOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiN{KgbJJ{KGK7IGfTWE0yKG2ndHjv[EwhUUN3ME2wMlM5KM7:TR?= Mn3GNlY{PTV3M{K=
human KBVIN cells NGTtVY9EgXSxdH;4bYPDqGG|c3H5 MYW3NkBp M{nJfWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDXkmQIHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUC9NE41KM7:TR?= MXSyOVQ{QDd4OR?=
human GBM1 cells NWjkZXk5WHKxbHnm[ZJifGmxbjDhd5NigQ>? NVexR|B[PzJiaB?= NFvSUWNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFfCUVEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JHdUXC1zIH3leIhw\CxiSVO1NF0xNjR4IN88US=> M{LyZ|I3OzV3NUOy
human U937 cells M3HrXWN6fG:2b4jpZ:Kh[XO|YYm= NXvTUFE5PDhiaB?= MnThR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{BmgHC{ZYPzbY5oKHB3MzDteZRidnRiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSExvJygTWM2OD1zLkGg{txO M{DMUVE6PzF3M{G5

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents individually and in order:
Saline
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID