Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells MkPxR5l1d3SxeHnjxsBie3OjeR?= MVi3NkBp NXrl[HpUS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBq[zVyPUGgcm0> MX2yNFM4OTF6Mx?=
human Bel7402 cells M4j3PGN6fG:2b4jpZ:Kh[XO|YYm= M{jDVVk3KGh? MYXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU4zKG6P M3G0dlE2OTZ3MUS0
human HCT8 cells NH7ieWlEgXSxdH;4bYPDqGG|c3H5 MV65OkBp MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3Q5KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT61JI5O M1rQTlE2OTZ3MUS0
human MCF7 cells NGC2TldEgXSxdH;4bYPDqGG|c3H5 MXS5OkBp NIq0WnNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU45KG6P M{f5XFE2OTZ3MUS0
human A549 cells NFzSSXZEgXSxdH;4bYPDqGG|c3H5 M1rublk3KGh? MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mz6yJI5O M3rPfVE2OTZ3MUS0
human colon carcinoma SW620 cell NHfkbolEgXSxdH;4bYPDqGG|c3H5 Mn7IR5l1d3SxeHnjJJBwfGWwdHnheIlwdiCxZjDUc5BwfGWlYX6gLHRRMSCkeTD0bIUh[2:vcH;1coQhcW5iaIXtZY4h[2:ub36gZ4Fz[2mwb33hJHNYPjJyIHPlcIwhdGmwZTygTWM2OD1|LkKgcm0> MVOxNlQxQDdyNx?=
human MESSA cells NUjxTopTS3m2b4TvfIlkyqCjc4PhfS=> NHLGTXc4OiCq M{jscmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1GW1ODIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME20JI5O NWHTV4JHOjF|NEG2O|Q>
human BGC823 cells NXv4cmp7S3m2b4TvfIlkyqCjc4PhfS=> NGizOnA6PiCq NEXJcotEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDT0N6MkOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01NjNibl2= NFG0WlMyPTF4NUG0OC=>
human Ketr3 cells MmjIR5l1d3SxeHnjxsBie3OjeR?= MkLnPVYhcA>? NGnWdpBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBM\XS{MzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQvQSCwTR?= M2njVVE2OTZ3MUS0
LOX cells NFzrOXlEgXSxdH;4bYPDqGG|c3H5 MUPDfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hVE:[IHPlcIx{KCivZXzhco9u[Slib3[gbJVu[W5idIXtc5Ih[2WubDDsbY5meyCkeTD1d4lv\yCPVGSgZZN{[XluIFnDOVA:PSCwTR?= NYnuSIdnPzh3M{OzNS=>
human A549 cells MoDOSpVv[3Srb36gZZN{[Xl? MlPVOEBp NF3LNnVCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IESgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD13IH7N NWjjTnk4OTh{MEe3OFg>
human H69 cells MV7DfZRwfG:6aXRCpIF{e2G7 NFjObmc4OiCq NHvNb2FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPjliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[gcm0> NHv1ZVEzOTN2MU[3OC=>
human A2780 cells NWXJ[GhWS3m2b4TvfIlkyqCjc4PhfS=> M2nxVlczKGh? MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDhcJBp[TWkZYThN{BqdnSnZ4LpckBie3Onc4Pl[EBieyClZXzsJJN2en[rdnHsJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;NjDuUS=> MnHKNlI6PTl{NE[=
human LNCAP cells MnPOR5l1d3SxeHnjxsBie3OjeR?= MXi3NkBp NE\aNFVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVkODUDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9PUBvVQ>? MYqyNVM1OTZ5NB?=
human HOP62 cells M4LJWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUm2T5FZT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUE:SNkKgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N Ml7INlU6ODl{N{m=
human MCF7 cells NGrV[4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVmyb2t[T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= Mn;JNlU6ODl{N{m=
human UACC62 cells M4PZ[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEPhV5FIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBWSUOFNkKgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N NXTaZVNxOjV7MEmyO|k>
human DU145 cells MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHTSOZJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= NV;Sb2R[OjV7MEmyO|k>
LoVo cell line NULvRoJ2S3m2b4TvfIlkyqCjc4PhfS=> M{XhZmN6fG:2b4jpZ{Bxd3SnboTpZZRqd25ib3[gWI9xd3SnY3HuJEhVWCliYomgeIhmKGOxbYDveY5lKGmwIDjoeY1idiClb3zvdoVkfGGuIHPhcoNmeiCOb2\vJINmdGxibHnu[UwhUUN3ME2xNU43KG6P NWjRN4xpOTJ2MEi3NFc>
human lymphoblast tumor cell line RPM18402 MXzDfZRwfG:6aXRCpIF{e2G7 M4PmWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx6dXCqb3LsZZN1KHS3bX;yJINmdGxibHnu[UBTWE1zOESwNkwhUUN3ME2xNkBvVQ>? NYDjTow1OTJ|OUK3OFU>
human HL60 cells MYrGeY5kfGmxbjDhd5NigQ>? NYH3bGw3SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{BjgSCVUlKgcYV1cG:mLDDJR|UxRTF{IH7N MWGxPVU1OTR6Mx?=
human A375 cells M4nWfmN6fG:2b4jpZ:Kh[XO|YYm= NFHaN2o4OiCq MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OD1zMzDuUS=> Ml60NlE{PDF4N{S=
COR-L23/P cell MYTDfZRwfG:6aXRCpIF{e2G7 M{LGc2N6fG:2b4jpZ4l1gSCvZXHzeZJm\CC3c3nu[{B1cGViQ1;SMWwzOyCyYYLlcpRidCBqQ1;SMWwzOy:SKTDoeY1idiCwb36gd41idGxiY3XscEBtfW6pIHPhdoNqdm:vYTDj[YxtKGyrbnWsJGlEPTB;MUOuNkBvVQ>? MoDqNVE5ODZ5MkS=
human NCI-H460 cells MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 MWW3NkBp M3fZTGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg1PjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JINwfWy2ZYKgZ492dnSncjDhcoFtgXOrczygTWM2OD1zNjDuUS=> M{CwSFIyPzh|M{[5
human MOLT4 cells Mn7lR5l1d3SxeHnjxsBie3OjeR?= NGqwc4Y4OiCq NInXdlhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOV0yWNDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE6KG6P NFHoVmgzPDlyMEeyOS=>
human LoVo cells NVnDXJNTS3m2b4TvfIlkyqCjc4PhfS=> NVHTTVdTPzJiaB?= MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkCgcm0> MkfoNlA{PzFzOEO=
RPM18402 tumor cell line M3\iN3Bzd2yrZnXyZZRqd25iYYPzZZk> NIXiR2VEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgZ4VtdCCycn;sbYZmemG2aX;uJIlvKFKSTUG4OFAzKHS3bX;yJINmdGxibHnu[UwhUUN3ME2yNEBvVQ>? NFq3VIkyPTR6MkmyPS=>
human SN12C cells NYjlT2I{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXfOWZFJT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW05zMlOgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDHTVUxRTJyIH7N NX7xcGZ1OjV7MEmyO|k>
human RPMI8226 cells M1zjd2N6fG:2b4jpZ:Kh[XO|YYm= MnHUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVnBOUTh{Mk[gZ4VtdHNiYomgUXRVKG2ndHjv[EwhUUN3ME2yNUBvVQ>? NXHpbnJqOTlyMUK5PVY>
HT-29 cells NV3JOYYzS3m2b4TvfIlkyqCjc4PhfS=> NFjvT|hEgXSxdH;4bYNqfHlid3HzJIRmfGW{bXnu[YQhcW5idnn0do8hcW5iSGStNlkh[2WubIOoZ49td25rIH;mJIh2dWGwIIT1cY9zKGOnbHygcIlv\XNiYomgeZNqdmdiTWTUJIF{e2G7LDDJR|UxRTJ3IH7N M{DtW|c5PTN|M{G=
SK-BR-3 cells NIjzVZFEgXSxdH;4bYPDqGG|c3H5 NID2NG1KdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZTCoV2suSlJvMzmsJGlEPTB;Mk[gcm0> M4r2UlEyOzN2NU[5
HeLa cells MkDQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUHHdo94fGhiaX7obYJqfGmxbjDv[kBJ\UyjIHPlcIx{KGGodHXyJFQh\GG7czygS2k2OD1|MDDuUS=> MV6xO|QyQDV6Mh?=
UACC 62 cells NEDsO2lEgXSxdH;4bYPDqGG|c3H5 Ml7wWIV{fGWmIHnuJJZqfHKxIH\vdkBkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB1fW2xcjDj[YxtKGyrbnWgWWFESyB4MjCocYVt[W6xbXGpMEBIUTVyPUOwJI5O NV;NPVBEOTB5MUS1NFI>
human HCT116 cells MlfXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHS5RlZIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJS1RzMU[gZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDHTVUxRTNyIH7N M1fJflI2QTB7Mke5
human lung cancer cell line (H128) NWrnPVlTS3m2b4TvfIlkyqCjc4PhfS=> NEDLZ3RKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBtfW6pIHPhcoNmeiClZXzsJIxqdmViKFixNlgqNCCLQ{WwQVMyKG6P MlLQNVE{OzR3Nkm=
human MES-SA/Dx5 cells MnriR5l1d3SxeHnjxsBie3OjeR?= NILUS4JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVNvU1GvSJg2KGOnbHzzJI93\XKneIDy[ZN{cW6pIF3EVlEh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUOzJI5O M4nVfFIyOzRzNke0
human A2780 cells MXHDfZRwfG:6aXRCpIF{e2G7 NYjLdWNtPzJiaB?= NHrTb4dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM{KG6P Mly1NlQ6ODB5MkW=
human MDA-MB-435 cells NWDicoQ1S3m2b4TvfIlkyqCjc4PhfS=> NHvlOlM4OiCq MofWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFfJOVA:OzRibl2= NUG4OXFrOjJ6NkewNVk>
human stomach cancer cell line (MKN45) NH7RSY5EgXSxdH;4bYPDqGG|c3H5 NEHsUnNKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB{fG:vYXPoJINidmOncjDj[YxtKGyrbnWgLG1MVjR3KTygTWM2OD1|ODDuUS=> MYixNVM{PDV4OR?=
human KB3-1 cells NU\6clNjS3m2b4TvfIlkyqCjc4PhfS=> MorPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2I{NTFiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUSwJI5O MmnyNVg5Ojl|M{S=
mouse P388 cells MUTDfZRwfG:6aXRCpIF{e2G7 Ml;PR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgVFM5QCClZXzsd{Bi\nSncjDNWHQh[XO|YYmsJGlEPTB;NEWgcm0> M3;teFE5QDJ7M{O0
A427 human lung carcinoma MknGVJJwdGmoZYLheIlwdiCjc4PhfS=> NHTI[IVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBu\WG|dYLl[EBi\2GrboP0JGE1OjdiaIXtZY4hdHWwZzDjZZJkcW6xbXGsJGlEPTB;NEmgcm0> NF60R5g6QDd4MUGx
human U251 cells NIXNTHZHfW6ldHnvckBie3OjeR?= M3\RSVYuOjRiaB?= NXLEUFNuUW6qaXLpeIlwdiCxZjD0c5BwcXOxbXXyZZNmNTFiaX6gbJVu[W5iVUK1NUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHj5dI95cWFvaX7keYNm\CCKSV[tNYFteGijIHHjZ5VufWyjdHnvckBqdiCwdXPs[YFzKGW6dILhZ5Qh[W[2ZYKgOkB1dyB{NDDodpMh[nliaX3teY5w[myxdDDhcoFtgXOrczygSWM2OD13NDDuUS=> NYSxXogyOjJ|MEW2NVI>
human colon cancer cell line (WiDr) MUDDfZRwfG:6aXRCpIF{e2G7 NUHIPZhsUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIwhdGmwZTCoW4lFeiluIFnDOVA:PTZibl2= MVSxNVM{PDV4OR?=
human HepG2 cells NI[y[ZREgXSxdH;4bYPDqGG|c3H5 NWS5Tm5FPCCmYYnz NYXn[G16S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KE2WVDDhd5NigSxiSVO1NF03OCCwTR?= NVzOdohmOTh3NUS5NFY>
human KB cells MlX0R5l1d3SxeHnjxsBie3OjeR?= NH3nWno4OiCq Mn7VR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KHO3bH\vdohw\GGvaX7lJGIh[2:ub4LpcYV1emmlIHHzd4F6NCCLQ{WwQVYzNjVibl2= NEniTYUzPTByOES1Oi=>
human HCT8 cells MUDDfZRwfG:6aXRCpIF{e2G7 M2rxSVMh\GG7cx?= NVT4PYRZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVcxKG6P M4HLcFIxOzl{NUS1
human GBM2 cells NGDqPZVRem:uaX\ldoF1cW:wIHHzd4F6 Moj4O|IhcA>? NIHNUJpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFfCUVIh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JHdUXC1zIH3leIhw\CxiSVO1NF0xNjF|IN88US=> NFr4UGkzPjN3NUWzNi=>
SK-MEL-2 cells M3TWVGN6fG:2b4jpZ:Kh[XO|YYm= MYW3NkBp NUXU[Y5qS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTBwMUSg{txO NVHIe|M6OjF|NEG2O|Q>
human IGROV1 cells MWLDfZRwfG:6aXRCpIF{e2G7 MYe3NkBp NX3nOG5xS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUUeUT2[xJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NF0xNjF4IN88US=> M4\PfFIyOzRzNke0
human U87 cells M4[zV3Bzd2yrZnXyZZRqd25iYYPzZZk> MoPnOFghcA>? MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMU[g{txO MWCyOFgzPjhzOB?=
human HT1080 cells M3XrWXBzd2yrZnXyZZRqd25iYYPzZZk> NXzBdldpPDhiaB?= NUjTfVRMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWFExQDBiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygTWM2OD1yLkG4JO69VQ>? M1\tNlI3PDB6OEG1
human Hep3B cells Ml\aVJJwdGmoZYLheIlwdiCjc4PhfS=> MVO3NkBp NGLZXohCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldFNDKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTB;MD6yNkDPxE1? MmHSNVk4QTZ7NU[=
human GBM3 cells NGTrWIVRem:uaX\ldoF1cW:wIHHzd4F6 M3fy[|czKGh? MoGyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCJQl2zJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDXV3QuOSCvZYToc4QtKEmFNUC9NE4{QCEQvF2= Mn\aNlY{PTV3M{K=
human KBVIN cells MVzDfZRwfG:6aXRCpIF{e2G7 MXi3NkBp NEPZ[YREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSl[LTjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvPCEQvF2= M3vSZlI2PDN6N{[5
human GBM1 cells M3vGVnBzd2yrZnXyZZRqd25iYYPzZZk> NWXpSHFIPzJiaB?= MV\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEeETUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiN{KgbJJ{KGK7IGfTWE0yKG2ndHjv[EwhUUN3ME2wMlQ3KM7:TR?= NVS0RpFuOjZ|NUW1N|I>
human U937 cells MUfDfZRwfG:6aXRCpIF{e2G7 NVv4WG9yPDhiaB?= M2nQ[2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHU6OzdiY3XscJMh\XiycnXzd4lv\yCyNUOgcZV1[W62IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[Xli78{MJGlEPTB;MT6xJO69VQ>? MXWxPVcyPTNzOR?=

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID