Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells M2H2ZmN6fG:2b4jpZ:Kh[XO|YYm= M2fNXFczKGh? MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIHnjOVA:OSCwTR?= M2XMVlIxOzdzMUiz
human Bel7402 cells MWHDfZRwfG:6aXRCpIF{e2G7 MlXTPVYhcA>? NYD0NZV1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSmWuN{SwNkBk\WyuczDh[pRmeiB7NjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTFwMjDuUS=> NH23SYoyPTF4NUG0OC=>
human HCT8 cells M{nHRWN6fG:2b4jpZ:Kh[XO|YYm= Mo\wPVYhcA>? NFTiT|lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1R6IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU42KG6P NFfiWWcyPTF4NUG0OC=>
human MCF7 cells NX3xeWZMS3m2b4TvfIlkyqCjc4PhfS=> NFzDbYg6PiCq Moq2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuPEBvVQ>? MmfpNVUyPjVzNES=
human A549 cells NHXFeohEgXSxdH;4bYPDqGG|c3H5 MnvhPVYhcA>? MoDYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUOuNkBvVQ>? MVqxOVE3PTF2NB?=
human colon carcinoma SW620 cell M1HHOGN6fG:2b4jpZ:Kh[XO|YYm= NE\BcZREgXSxdH;4bYMheG:2ZX70bYF1cW:wIH;mJHRweG:2ZXPhckApXFBrIHL5JJRp\SClb33wc5Vv\CCrbjDoeY1idiClb3zvckBk[XKlaX7vcYEhW1d4MkCgZ4VtdCCuaX7lMEBKSzVyPUOuNkBvVQ>? MlziNVI1ODh5MEe=
human MESSA cells MlvNR5l1d3SxeHnjxsBie3OjeR?= MVK3NkBp MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSXNUSSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;NDDuUS=> M4LqZ|IyOzRzNke0
human BGC823 cells M4n2W2N6fG:2b4jpZ:Kh[XO|YYm= NGLzd3o6PiCq NWO2Oo9lS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkeFOEKzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PC5|IH7N MUWxOVE3PTF2NB?=
human Ketr3 cells NGXhWXNEgXSxdH;4bYPDqGG|c3H5 NHfON4g6PiCq M{[5T2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtmfHJ|IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE46KG6P M1nzVVE2OTZ3MUS0
LOX cells NYLhXXlES3m2b4TvfIlkyqCjc4PhfS=> MXvDfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hVE:[IHPlcIx{KCivZXzhco9u[Slib3[gbJVu[W5idIXtc5Ih[2WubDDsbY5meyCkeTD1d4lv\yCPVGSgZZN{[XluIFnDOVA:PSCwTR?= NFOxZ2U4QDV|M{Ox
human A549 cells NFfieWdHfW6ldHnvckBie3OjeR?= MmfwOEBp M3uwdmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiNDDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTVibl2= M4DndlE5OjB5N{S4
human H69 cells M3Lh[2N6fG:2b4jpZ:Kh[XO|YYm= MXG3NkBp NFHMfYtEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPjliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[gcm0> MmHENlE{PDF4N{S=
human A2780 cells M2HseWN6fG:2b4jpZ:Kh[XO|YYm= NIDMdWU4OiCq MoT1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMhd3[ncnX4dJJme3OrbnegZYxxcGF3YnX0ZVMhcW62ZXfybY4h[XO|ZYPz[YQh[XNiY3XscEB{fXK4aY\hcEBi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPU[gcm0> MkXNNlI6PTl{NE[=
human LNCAP cells M3riXGN6fG:2b4jpZ:Kh[XO|YYm= M2XBTlczKGh? NEnZUoJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVkODUDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9PUBvVQ>? M4rTelIyOzRzNke0
human HOP62 cells NH7oVnZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnJS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTG9RPjJiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCLQ{WwQVExKG6P MYGyOVkxQTJ5OR?=
human MCF7 cells Mlr2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUnGTGM4T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= M3jMN|I2QTB7Mke5
human UACC62 cells NUjDdGRCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MofnS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gWWFESzZ{IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= MYWyOVkxQTJ5OR?=
human DU145 cells MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX6yfpJRT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hTFVzNEWgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N MWqyOVkxQTJ5OR?=
LoVo cell line NWnsbZVtS3m2b4TvfIlkyqCjc4PhfS=> M4jFXWN6fG:2b4jpZ{Bxd3SnboTpZZRqd25ib3[gWI9xd3SnY3HuJEhVWCliYomgeIhmKGOxbYDveY5lKGmwIDjoeY1idiClb3zvdoVkfGGuIHPhcoNmeiCOb2\vJINmdGxibHnu[UwhUUN3ME2xNU43KG6P NWDjdHgyOTJ2MEi3NFc>
human lymphoblast tumor cell line RPM18402 MlLWR5l1d3SxeHnjxsBie3OjeR?= MoL0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gcJlueGixYnzhd5QhfHWvb4KgZ4VtdCCuaX7lJHJRVTF6NECyMEBKSzVyPUGyJI5O M4TZSFEzOzl{N{S1
human HL60 cells NGjjRmRHfW6ldHnvckBie3OjeR?= NEPB[JZCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KFOUQjDt[ZRpd2RuIFnDOVA:OTJibl2= MWGxPVU1OTR6Mx?=
human A375 cells MnzBR5l1d3SxeHnjxsBie3OjeR?= M16zc|czKGh? NUK1VIJxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTN5NTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NVMhdk1? M17ZO|IyOzRzNke0
COR-L23/P cell NFfvUVREgXSxdH;4bYPDqGG|c3H5 MnXjR5l1d3SxeHnjbZR6KG2nYYP1doVlKHW|aX7nJJRp\SCFT2KtUFI{KHCjcnXueIFtKCiFT2KtUFI{N1BrIHj1cYFvKG6xbjDzcYFtdCClZXzsJIx2dmdiY3HyZ4lvd22jIHPlcIwhdGmwZTygTWM2OD1zMz6yJI5O MnnvNVE5ODZ5MkS=
human NCI-H460 cells M1rW[nBzd2yrZnXyZZRqd25iYYPzZZk> M2TzXlczKGh? MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc5VtfGW{IHPveY51\XJiYX7hcJl{cXNuIFnDOVA:OTZibl2= MViyNVc5OzN4OR?=
human MOLT4 cells M1;vTmN6fG:2b4jpZ:Kh[XO|YYm= MmPKO|IhcA>? NUf3bWNVS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVU:OVESgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQSCwTR?= MW[yOFkxODd{NR?=
human LoVo cells NXLpNIhIS3m2b4TvfIlkyqCjc4PhfS=> M3;QRVczKGh? M3X2WGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxwXm9iY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yNEBvVQ>? NIWyS5UzODN5MUG4Ny=>
RPM18402 tumor cell line M{XiPXBzd2yrZnXyZZRqd25iYYPzZZk> NYfJSFNNS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JINmdGxicILvcIln\XKjdHnvckBqdiCUUF2xPFQxOiC2dX3vdkBk\WyuIHzpcoUtKEmFNUC9NlAhdk1? NHnaO4YyPTR6MkmyPS=>
human SN12C cells Mk\iS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnjYS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV24yOkNiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCJSUWwQVIxKG6P NHS2U5kzPTlyOUK3PS=>
human RPMI8226 cells NX\YTWcyS3m2b4TvfIlkyqCjc4PhfS=> MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSVG1KQDJ{NjDj[YxteyCkeTDNWHQhdWW2aH;kMEBKSzVyPUKxJI5O M1LSSVE6ODF{OUm2
HT-29 cells NHTQUVREgXSxdH;4bYPDqGG|c3H5 NIrLPWxEgXSxdH;4bYNqfHlid3HzJIRmfGW{bXnu[YQhcW5idnn0do8hcW5iSGStNlkh[2WubIOoZ49td25rIH;mJIh2dWGwIIT1cY9zKGOnbHygcIlv\XNiYomgeZNqdmdiTWTUJIF{e2G7LDDJR|UxRTJ3IH7N MVW3PFU{OzNz
SK-BR-3 cells NXvDeHJwS3m2b4TvfIlkyqCjc4PhfS=> MUPJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHygcIlv\SBqU1utRnIuOyluIFnDOVA:OjZibl2= NWPnNIduOTF|M{S1Olk>
HeLa cells M2XpSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlHuS5Jwf3SqIHnubIljcXSrb36gc4YhUGWOYTDj[YxteyCjZoTldkA1KGSjeYOsJGdKPTB;M{Cgcm0> NWX2[FFnOTd2MUi1PFI>
UACC 62 cells NUXRdFVmS3m2b4TvfIlkyqCjc4PhfS=> MmnmWIV{fGWmIHnuJJZqfHKxIH\vdkBkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB1fW2xcjDj[YxtKGyrbnWgWWFESyB4MjCocYVt[W6xbXGpMEBIUTVyPUOwJI5O NYr5VmRzOTB5MUS1NFI>
human HCT116 cells M{Dqfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnvES5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGNVOTF4IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiR1m1NF0{OCCwTR?= NWS2XI05OjV7MEmyO|k>
human lung cancer cell line (H128) MlrSR5l1d3SxeHnjxsBie3OjeR?= NHvtU3NKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBtfW6pIHPhcoNmeiClZXzsJIxqdmViKFixNlgqNCCLQ{WwQVMyKG6P MXSxNVM{PDV4OR?=
human MES-SA/Dx5 cells MWPDfZRwfG:6aXRCpIF{e2G7 NEfSWFZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVNvU1GvSJg2KGOnbHzzJI93\XKneIDy[ZN{cW6pIF3EVlEh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUOzJI5O NXHyZm03OjF|NEG2O|Q>
human A2780 cells M1vVcGN6fG:2b4jpZ:Kh[XO|YYm= NYLJc2Q{PzJiaB?= MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUOzJI5O NEHsZWUzPDlyMEeyOS=>
human MDA-MB-435 cells MVnDfZRwfG:6aXRCpIF{e2G7 MnLlO|IhcA>? MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHnuZ5Vj[XSnZDDmc5IhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NF0{PCCwTR?= NFfxVJMzOjh4N{CxPS=>
human stomach cancer cell line (MKN45) NHjhN4VEgXSxdH;4bYPDqGG|c3H5 M{HRfGlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJJN1d22jY3igZ4Fv[2W{IHPlcIwhdGmwZTCoUWtPPDVrLDDJR|UxRTN6IH7N M3\jUFEyOzN2NU[5
human KB3-1 cells MYjDfZRwfG:6aXRCpIF{e2G7 MkPvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2I{NTFiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUSwJI5O NV[2SWRXOTh6MkmzN|Q>
mouse P388 cells M1nHfWN6fG:2b4jpZ:Kh[XO|YYm= M2m1R2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJHA{QDhiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUS1JI5O MVKxPFgzQTN|NB?=
A427 human lung carcinoma MkfoVJJwdGmoZYLheIlwdiCjc4PhfS=> NFGyVYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBu\WG|dYLl[EBi\2GrboP0JGE1OjdiaIXtZY4hdHWwZzDjZZJkcW6xbXGsJGlEPTB;NEmgcm0> MmWxPVg4PjFzMR?=
human U251 cells MnTwSpVv[3Srb36gZZN{[Xl? NG\QTYU3NTJ2IHi= NXfNTIZuUW6qaXLpeIlwdiCxZjD0c5BwcXOxbXXyZZNmNTFiaX6gbJVu[W5iVUK1NUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHj5dI95cWFvaX7keYNm\CCKSV[tNYFteGijIHHjZ5VufWyjdHnvckBqdiCwdXPs[YFzKGW6dILhZ5Qh[W[2ZYKgOkB1dyB{NDDodpMh[nliaX3teY5w[myxdDDhcoFtgXOrczygSWM2OD13NDDuUS=> Mn;ZNlI{ODV4MUK=
human colon cancer cell line (WiDr) NGjve4FEgXSxdH;4bYPDqGG|c3H5 NXWzUox1UW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIwhdGmwZTCoW4lFeiluIFnDOVA:PTZibl2= NYDDWnVJOTF|M{S1Olk>
human HepG2 cells M2i1VmN6fG:2b4jpZ:Kh[XO|YYm= Mmq0OEBl[Xm| NXfZTJVXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KE2WVDDhd5NigSxiSVO1NF03OCCwTR?= M3e0VVE5PTV2OUC2
human KB cells Mnv6R5l1d3SxeHnjxsBie3OjeR?= NH3vTlA4OiCq M1vtW2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IEeyJIhzeyC|dXzmc5Jpd2SjbXnu[UBDKGOxbH;ybY1mfHKrYzDhd5NigSxiSVO1NF03Oi53IH7N NHvHSoQzPTByOES1Oi=>
human HCT8 cells MYHDfZRwfG:6aXRCpIF{e2G7 Moe3N{Bl[Xm| MmrtR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVQCClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUewJI5O M4TUfFIxOzl{NUS1
human GBM2 cells MWfQdo9tcW[ncnH0bY9vKGG|c3H5 NHnHZY04OiCq MoizRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCJQl2yJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDXV3QuOSCvZYToc4QtKEmFNUC9NE4yOyEQvF2= NIHaTmYzPjN3NUWzNi=>
SK-MEL-2 cells M{fib2N6fG:2b4jpZ:Kh[XO|YYm= MV23NkBp NVnUd452S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTBwMUSg{txO MXiyNVM1OTZ5NB?=
human IGROV1 cells NWTnbGExS3m2b4TvfIlkyqCjc4PhfS=> NGfURoc4OiCq MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDJS3JQXjFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUCuNVYh|ryP M3LId|IyOzRzNke0
human U87 cells NET3ZXVRem:uaX\ldoF1cW:wIHHzd4F6 MX20PEBp NIXqPGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW4O{Bk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNVYh|ryP NInIfmgzPDh{NkixPC=>
human HT1080 cells M2Xv[XBzd2yrZnXyZZRqd25iYYPzZZk> M2XwblQ5KGh? NEmwendCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUNVA5OCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDJR|UxRTBwMUig{txO MUOyOlQxQDhzNR?=
human Hep3B cells MVzQdo9tcW[ncnH0bY9vKGG|c3H5 MYG3NkBp NUCyNIZFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZA{SiClZXzsd{Bi\nSncjC3NkBpenNiYomgXHRVKGG|c3H5MEBIUTVyPUCuNlIh|ryP M3fZeVE6Pzl4OUW2
human GBM3 cells MkLwVJJwdGmoZYLheIlwdiCjc4PhfS=> M{PU[FczKGh? M1juWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOzhizszN NXPOc3lPOjZ|NUW1N|I>
human KBVIN cells M3ryNmN6fG:2b4jpZ:Kh[XO|YYm= NEDaPZc4OiCq NVTmfVFwS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0KYSV6gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjRizszN NFvmXVYzPTR|OEe2PS=>
human GBM1 cells NHfxW|hRem:uaX\ldoF1cW:wIHHzd4F6 NXjlPFE{PzJiaB?= M{\HXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvPDZizszN NUj1U4gzOjZ|NUW1N|I>
human U937 cells MX7DfZRwfG:6aXRCpIF{e2G7 NVWxfIxiPDhiaB?= M1LEU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHU6OzdiY3XscJMh\XiycnXzd4lv\yCyNUOgcZV1[W62IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[Xli78{MJGlEPTB;MT6xJO69VQ>? M{fNfVE6PzF3M{G5

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID