Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NHPDR|NEgXSxdH;4bYPDqGG|c3H5 Mn7MO|IhcA>? MoLKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDpZ|UxRTFibl2= NVX6[YUzOjB|N{GxPFM>
human Bel7402 cells NXHvdWxzS3m2b4TvfIlkyqCjc4PhfS=> NX3qUZZSQTZiaB?= NF7zWlZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NECyJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS5{IH7N M4PMUVE2OTZ3MUS0
human HCT8 cells NXu2elg1S3m2b4TvfIlkyqCjc4PhfS=> M1TjOlk3KGh? MmHUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVQCClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuOUBvVQ>? Ml\RNVUyPjVzNES=
human MCF7 cells NH\FNJlEgXSxdH;4bYPDqGG|c3H5 M4PmRVk3KGh? NHLScmpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU45KG6P M{G5dVE2OTZ3MUS0
human A549 cells M1zOZWN6fG:2b4jpZ:Kh[XO|YYm= Mn2xPVYhcA>? MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mz6yJI5O MoDhNVUyPjVzNES=
human colon carcinoma SW620 cell MXjDfZRwfG:6aXRCpIF{e2G7 NHj2S|dEgXSxdH;4bYMheG:2ZX70bYF1cW:wIH;mJHRweG:2ZXPhckApXFBrIHL5JJRp\SClb33wc5Vv\CCrbjDoeY1idiClb3zvckBk[XKlaX7vcYEhW1d4MkCgZ4VtdCCuaX7lMEBKSzVyPUOuNkBvVQ>? M3XZVFEzPDB6N{C3
human MESSA cells MkLCR5l1d3SxeHnjxsBie3OjeR?= MoHRO|IhcA>? NV\TUGhsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUWVU1GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVQhdk1? NXW4[WRvOjF|NEG2O|Q>
human BGC823 cells M1y4VGN6fG:2b4jpZ:Kh[XO|YYm= MX25OkBp NETTOoVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDT0N6MkOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01NjNibl2= MlH5NVUyPjVzNES=
human Ketr3 cells M1ThZ2N6fG:2b4jpZ:Kh[XO|YYm= MVW5OkBp Mn3FR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT4V1ejNiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20Mlkhdk1? NXjUcGF7OTVzNkWxOFQ>
LOX cells MVPDfZRwfG:6aXRCpIF{e2G7 MY\DfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hVE:[IHPlcIx{KCivZXzhco9u[Slib3[gbJVu[W5idIXtc5Ih[2WubDDsbY5meyCkeTD1d4lv\yCPVGSgZZN{[XluIFnDOVA:PSCwTR?= NULKW5IxPzh3M{OzNS=>
human A549 cells MkLDSpVv[3Srb36gZZN{[Xl? NXzuWVFEPCCq NGWwcYNCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IESgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD13IH7N MnH0NVgzODd5NEi=
human H69 cells NUjSe5BPS3m2b4TvfIlkyqCjc4PhfS=> M4TTb|czKGh? NV;rNYNtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDZ7IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME22JI5O MXuyNVM1OTZ5NB?=
human A2780 cells MofBR5l1d3SxeHnjxsBie3OjeR?= MV23NkBp NV\BXW9NS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNib4\ldoV5eHKnc4Ppcoch[WyyaHG1ZoV1[TNiaX70[YdzcW5iYYPz[ZN{\WRiYYOgZ4VtdCC|dYL2bZZidCCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVYhdk1? Ml[1NlI6PTl{NE[=
human LNCAP cells NHzHNZFEgXSxdH;4bYPDqGG|c3H5 MV63NkBp M3G4WGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME25JI5O MnriNlE{PDF4N{S=
human HOP62 cells M3TNXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2fueWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhQWDZ{IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= MUeyOVkxQTJ5OR?=
human MCF7 cells NGKzVJNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MljVS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWNHPyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEmFNUC9NVAhdk1? NYLrUlB1OjV7MEmyO|k>
human UACC62 cells Mn3jS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlfrS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gWWFESzZ{IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= NIjkeHozPTlyOUK3PS=>
human DU145 cells M3fDR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2XORmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGRWOTR3IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= NV3pSmt4OjV7MEmyO|k>
LoVo cell line NFf3S4xEgXSxdH;4bYPDqGG|c3H5 MYTDfZRwfG:6aXOgdI91\W62aXH0bY9vKG:oIGTvdI91\WOjbjCoWHAqKGK7IITo[UBkd22yb4Xu[EBqdiBqaIXtZY4h[2:ub4LlZ5RidCClYX7j[ZIhVG:YbzDj[YxtKGyrbnWsJGlEPTB;MUGuOkBvVQ>? MWexNlQxQDdyNx?=
human lymphoblast tumor cell line RPM18402 MojrR5l1d3SxeHnjxsBie3OjeR?= MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDsfY1xcG:kbHHzeEB1fW2xcjDj[YxtKGyrbnWgVnBOOTh2MEKsJGlEPTB;MUKgcm0> MVexNlM6Ojd2NR?=
human HL60 cells MlHHSpVv[3Srb36gZZN{[Xl? NIXEUnFCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KFOUQjDt[ZRpd2RuIFnDOVA:OTJibl2= NFvTdogyQTV2MUS4Ny=>
human A375 cells MXvDfZRwfG:6aXRCpIF{e2G7 NYjTR4ZqPzJiaB?= NUjIfW9RS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTN5NTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NVMhdk1? MkTnNlE{PDF4N{S=
COR-L23/P cell MVrDfZRwfG:6aXRCpIF{e2G7 NWS3OXlDS3m2b4TvfIlkcXS7IH3lZZN2emWmIIXzbY5oKHSqZTDDU3IuVDJ|IIDhdoVvfGGuIDjDU3IuVDJ|L2CpJIh2dWGwIH7vckB{dWGubDDj[YxtKGy3bnegZ4Fz[2mwb33hJINmdGxibHnu[UwhUUN3ME2xN{4zKG6P MofjNVE5ODZ5MkS=
human NCI-H460 cells MoG3VJJwdGmoZYLheIlwdiCjc4PhfS=> NGDXWmM4OiCq MVrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDjc5VtfGW{IHPveY51\XJiYX7hcJl{cXNuIFnDOVA:OTZibl2= MXmyNVc5OzN4OR?=
human MOLT4 cells NHTPXGdEgXSxdH;4bYPDqGG|c3H5 MoLZO|IhcA>? MoXNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUW9NXDRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPUBvVQ>? MoS1NlQ6ODB5MkW=
human LoVo cells NXX4[|FGS3m2b4TvfIlkyqCjc4PhfS=> NVXMTG5YPzJiaB?= MojqR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUI9XdyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKwJI5O NXm1VZBLOjB|N{GxPFM>
RPM18402 tumor cell line MXTQdo9tcW[ncnH0bY9vKGG|c3H5 NE\JRYtEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgZ4VtdCCycn;sbYZmemG2aX;uJIlvKFKSTUG4OFAzKHS3bX;yJINmdGxibHnu[UwhUUN3ME2yNEBvVQ>? M{DxelE2PDh{OUK5
human SN12C cells NVfFSId2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{\PT2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHNPOTKFIHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiR1m1NF0zOCCwTR?= NYfI[JNlOjV7MEmyO|k>
human RPMI8226 cells NGTmTZREgXSxdH;4bYPDqGG|c3H5 MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSVG1KQDJ{NjDj[YxteyCkeTDNWHQhdWW2aH;kMEBKSzVyPUKxJI5O MX:xPVAyOjl7Nh?=
HT-29 cells M1S3VmN6fG:2b4jpZ:Kh[XO|YYm= MVzDfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hUFRvMkmgZ4VtdHNqY3;sc44qKG:oIHj1cYFvKHS3bX;yJINmdGxibHnu[ZMh[nlidYPpcochVVSWIHHzd4F6NCCLQ{WwQVI2KG6P NXzoRWU1Pzh3M{OzNS=>
SK-BR-3 cells M{XUZ2N6fG:2b4jpZ:Kh[XO|YYm= NYOxU2Z3UW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[nKnYYP0JINidmOncjDj[YxtKGyrbnWgLHNMNUKULUOpMEBKSzVyPUK2JI5O MkTXNVE{OzR3Nkm=
HeLa cells MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUDHdo94fGhiaX7obYJqfGmxbjDv[kBJ\UyjIHPlcIx{KGGodHXyJFQh\GG7czygS2k2OD1|MDDuUS=> MmWxNVc1OTh3OEK=
UACC 62 cells MkLSR5l1d3SxeHnjxsBie3OjeR?= MnLrWIV{fGWmIHnuJJZqfHKxIH\vdkBkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB1fW2xcjDj[YxtKGyrbnWgWWFESyB4MjCocYVt[W6xbXGpMEBIUTVyPUOwJI5O M4XiVVExPzF2NUCy
human HCT116 cells MnX0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX;sZnc5T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IHL5JIZqfmVvZH;z[UBoem:5dHigbY5pcWKrdHnvckBie3OjeTygS2k2OD1|MDDuUS=> NHHv[lIzPTlyOUK3PS=>
human lung cancer cell line (H128) NXH3ZZhCS3m2b4TvfIlkyqCjc4PhfS=> NUfwbXJEUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdHWwZzDjZY5k\XJiY3XscEBtcW6nIDjINVI5MSxiSVO1NF0{OSCwTR?= M4HKblEyOzN2NU[5
human MES-SA/Dx5 cells M17CSWN6fG:2b4jpZ:Kh[XO|YYm= MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSXMuW0FxRIi1JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKE2GUkGgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTN|IH7N MV6yNVM1OTZ5NB?=
human A2780 cells NEnoR4lEgXSxdH;4bYPDqGG|c3H5 MYe3NkBp Mmr5R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zN{BvVQ>? M4C2[VI1QTByN{K1
human MDA-MB-435 cells NGTOemNEgXSxdH;4bYPDqGG|c3H5 NHvBWFg4OiCq MnHLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFfJOVA:OzRibl2= Mo\iNlI5PjdyMUm=
human stomach cancer cell line (MKN45) NIjW[GZEgXSxdH;4bYPDqGG|c3H5 MUTJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDzeI9u[WOqIHPhcoNmeiClZXzsJIxqdmViKF3LUlQ2MSxiSVO1NF0{QCCwTR?= M1rETVEyOzN2NU[5
human KB3-1 cells NXv3b4NiS3m2b4TvfIlkyqCjc4PhfS=> M1v0SGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDOy1zIHPlcIx{KGGodHXyJG1VXCCjc4PhfUwhUUN3ME20NEBvVQ>? MXexPFgzQTN|NB?=
mouse P388 cells MUXDfZRwfG:6aXRCpIF{e2G7 M4m5Z2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJHA{QDhiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUS1JI5O M{XsOVE5QDJ7M{O0
A427 human lung carcinoma NIfQVYpRem:uaX\ldoF1cW:wIHHzd4F6 M37O[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JI1m[XO3cnXkJIFo[Wmwc4SgRVQzPyCqdX3hckBtfW6pIHPhdoNqdm:vYTygTWM2OD12OTDuUS=> MoXOPVg4PjFzMR?=
human U251 cells NFG2[IpHfW6ldHnvckBie3OjeR?= NIq0WnM3NTJ2IHi= MVrJcohq[mm2aX;uJI9nKHSxcH;pd49u\XKjc3WtNUBqdiCqdX3hckBWOjVzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcHmyb4jpZU1qdmS3Y3XkJGhKTi1zYXzwbIEh[WOldX31cIF1cW:wIHnuJI52[2ynYYKg[Zh1emGldDDh[pRmeiB4IITvJFI1KGi{czDifUBqdW23bn;icI91KGGwYXz5d4l{NCCHQ{WwQVU1KG6P MUWyNlMxPTZzMh?=
human colon cancer cell line (WiDr) MUjDfZRwfG:6aXRCpIF{e2G7 MXvJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubDDsbY5mKCiZaVTyLUwhUUN3ME21OkBvVQ>? MVexNVM{PDV4OR?=
human HepG2 cells NW\qdINSS3m2b4TvfIlkyqCjc4PhfS=> MWm0JIRigXN? NGOw[HNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA1KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVYxKG6P MVSxPFU2PDlyNh?=
human KB cells NXLrdJR3S3m2b4TvfIlkyqCjc4PhfS=> MYG3NkBp NFG1VFBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSiClZXzsd{Bi\nSncjC3NkBpenNic4Xs[o9zcG:mYX3pcoUhSiClb3zvdolu\XS{aXOgZZN{[XluIFnDOVA:PjJwNTDuUS=> NYXwcVdqOjVyMEi0OVY>
human HCT8 cells MXPDfZRwfG:6aXRCpIF{e2G7 NWHYWmM{OyCmYYnz MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3Q5KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;N{Cgcm0> MYqyNFM6OjV2NR?=
human GBM2 cells MYfQdo9tcW[ncnH0bY9vKGG|c3H5 M4X5dFczKGh? M4nNOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOTNizszN MYqyOlM2PTV|Mh?=
SK-MEL-2 cells M1[ybWN6fG:2b4jpZ:Kh[XO|YYm= MVS3NkBp MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMjDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NE4yPCEQvF2= M2frSVIyOzRzNke0
human IGROV1 cells NInTN25EgXSxdH;4bYPDqGG|c3H5 MWO3NkBp NGq5WYlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBKT1KRVkGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvOTZizszN M{nSOVIyOzRzNke0
human U87 cells NX;mb3pHWHKxbHnm[ZJifGmxbjDhd5NigQ>? NWjLXlZ7PDhiaB?= NV\mXFFqSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFch[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlE3KM7:TR?= MVyyOFgzPjhzOB?=
human HT1080 cells NGHR[GRRem:uaX\ldoF1cW:wIHHzd4F6 MVm0PEBp MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwQVAvOThizszN MmTyNlY1ODh6MUW=
human Hep3B cells NXfWdm5rWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVO3NkBp MnvqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYCzRkBk\WyuczDh[pRmeiB5MjDodpMh[nliWGTUJIF{e2G7LDDHTVUxRTBwMkKg{txO MV:xPVc6Pjl3Nh?=
human GBM3 cells NVu2WHFSWHKxbHnm[ZJifGmxbjDhd5NigQ>? NHvLTpY4OiCq M4i3T2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOzhizszN NGj2W3UzPjN3NUWzNi=>
human KBVIN cells MlHSR5l1d3SxeHnjxsBie3OjeR?= MWG3NkBp NGDJVYxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSl[LTjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvPCEQvF2= MU[yOVQ{QDd4OR?=
human GBM1 cells M1T1S3Bzd2yrZnXyZZRqd25iYYPzZZk> MWm3NkBp MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEeETUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiN{KgbJJ{KGK7IGfTWE0yKG2ndHjv[EwhUUN3ME2wMlQ3KM7:TR?= MUSyOlM2PTV|Mh?=
human U937 cells NHjI[WREgXSxdH;4bYPDqGG|c3H5 MV20PEBp MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIV5eHKnc4PpcocheDV|IH31eIFvfCCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6KO,:jDDJR|UxRTFwMTFOwG0> NGPJeZUyQTdzNUOxPS=>

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents individually and in order:
Saline
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID