Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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In DMSO USD 230 In stock
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USD 320 In stock
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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NXzqWZFvS3m2b4TvfIlkyqCjc4PhfS=> MY[3NkBp NVrPUmp2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBq[zVyPUGgcm0> NFG5OG8zODN5MUG4Ny=>
human Bel7402 cells Mn3OR5l1d3SxeHnjxsBie3OjeR?= NVPhZ3VlQTZiaB?= NIjac3FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NECyJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS5{IH7N M1;FT|E2OTZ3MUS0
human HCT8 cells M3zaeGN6fG:2b4jpZ:Kh[XO|YYm= MmnYPVYhcA>? NUPYRWpDS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvPSCwTR?= NHSxNoYyPTF4NUG0OC=>
human MCF7 cells NEnTZ|NEgXSxdH;4bYPDqGG|c3H5 NFm0c|I6PiCq M1fHVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xMlghdk1? NVHRVpd5OTVzNkWxOFQ>
human A549 cells MofBR5l1d3SxeHnjxsBie3OjeR?= NHLD[JM6PiCq NUXndZZHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVMvOiCwTR?= MYSxOVE3PTF2NB?=
human colon carcinoma SW620 cell MmTYR5l1d3SxeHnjxsBie3OjeR?= MWLDfZRwfG:6aXOgdI91\W62aXH0bY9vKG:oIGTvdI91\WOjbjCoWHAqKGK7IITo[UBkd22yb4Xu[EBqdiCqdX3hckBkd2yxbjDjZZJkcW6xbXGgV3c3OjBiY3XscEBtcW6nLDDJR|UxRTNwMjDuUS=> M{PvVFEzPDB6N{C3
human MESSA cells MX\DfZRwfG:6aXRCpIF{e2G7 NF25NIo4OiCq MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSXNUSSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;NDDuUS=> NEG0VW4zOTN2MU[3OC=>
human BGC823 cells MX;DfZRwfG:6aXRCpIF{e2G7 M3fCe|k3KGh? NUDPZlcxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkeFOEKzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PC5|IH7N M4PPXlE2OTZ3MUS0
human Ketr3 cells MX\DfZRwfG:6aXRCpIF{e2G7 NXLx[3J[QTZiaB?= M3K1OGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtmfHJ|IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE46KG6P NXvO[JRyOTVzNkWxOFQ>
LOX cells NEnQR4tEgXSxdH;4bYPDqGG|c3H5 NEXzfopEgXSxdH;4bYNqfHlid3HzJIRmfGW{bXnu[YQhcW5idnn0do8hcW5iTF;YJINmdGy|IDjt[Yxidm:vYTmgc4YhcHWvYX6geJVud3JiY3XscEBtcW6nczDifUB2e2mwZzDNWHQh[XO|YYmsJGlEPTB;NTDuUS=> NUnzbVN[Pzh3M{OzNS=>
human A549 cells MX\GeY5kfGmxbjDhd5NigQ>? M1j6OVQhcA>? NHLXVGRCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IESgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD13IH7N NX\3SGZCOTh{MEe3OFg>
human H69 cells NEfpOWpEgXSxdH;4bYPDqGG|c3H5 Mkf0O|IhcA>? MYjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIOlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTZibl2= MV2yNVM1OTZ5NB?=
human A2780 cells MYTDfZRwfG:6aXRCpIF{e2G7 MoS1O|IhcA>? M1\uZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJIFteGijNXLleIE{KGmwdHXndolvKGG|c3Xzd4VlKGG|IHPlcIwhe3W{dnn2ZYwh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME22JI5O NUjvZXlrOjJ7NUmyOFY>
human LNCAP cells NEfBT4JEgXSxdH;4bYPDqGG|c3H5 M1jQTlczKGh? MmfiR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUG5ESVBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUmgcm0> MV:yNVM1OTZ5NB?=
human HOP62 cells MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3fjZ2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhQWDZ{IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= M1;u[lI2QTB7Mke5
human MCF7 cells NV7Cb3V{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXXHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIJ6KG[rdnWt[I9{\SCpcn;3eIghcW6qaXLpeIlwdiCjc4PhfUwhUUN3ME2xNEBvVQ>? NH2wZo8zPTlyOUK3PS=>
human UACC62 cells NV\DUIkxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEjMOpBIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBWSUOFNkKgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N MljtNlU6ODl{N{m=
human DU145 cells MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUPiS2lFT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hTFVzNEWgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N M1\IRVI2QTB7Mke5
LoVo cell line NVnUWZdVS3m2b4TvfIlkyqCjc4PhfS=> MVrDfZRwfG:6aXOgdI91\W62aXH0bY9vKG:oIGTvdI91\WOjbjCoWHAqKGK7IITo[UBkd22yb4Xu[EBqdiBqaIXtZY4h[2:ub4LlZ5RidCClYX7j[ZIhVG:YbzDj[YxtKGyrbnWsJGlEPTB;MUGuOkBvVQ>? NFf1U3QyOjRyOEewOy=>
human lymphoblast tumor cell line RPM18402 MmXGR5l1d3SxeHnjxsBie3OjeR?= MV;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDsfY1xcG:kbHHzeEB1fW2xcjDj[YxtKGyrbnWgVnBOOTh2MEKsJGlEPTB;MUKgcm0> NHrNeYcyOjN7Mke0OS=>
human HL60 cells MkOwSpVv[3Srb36gZZN{[Xl? M{izcGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMOlAh[2WubIOgZpkhW1KEIH3leIhw\CxiSVO1NF0yOiCwTR?= MYmxPVU1OTR6Mx?=
human A375 cells NXTPdZdRS3m2b4TvfIlkyqCjc4PhfS=> NXvm[3lPPzJiaB?= NH3BOlZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOzd3IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME2xN{BvVQ>? NH7ldlUzOTN2MU[3OC=>
COR-L23/P cell NXPDNFJ7S3m2b4TvfIlkyqCjc4PhfS=> MXvDfZRwfG:6aXPpeJkhdWWjc4Xy[YQhfXOrbnegeIhmKEORUj3MNlMheGG{ZX70ZYwhMEORUj3MNlMwWCliaIXtZY4hdm:wIIPtZYxtKGOnbHygcJVv\yClYYLjbY5wdWFiY3XscEBtcW6nLDDJR|UxRTF|LkKgcm0> MmrCNVE5ODZ5MkS=
human NCI-H460 cells M1jj[HBzd2yrZnXyZZRqd25iYYPzZZk> MYe3NkBp NWDMSFR[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2:3bITldkBkd3WwdHXyJIFv[Wy7c3nzMEBKSzVyPUG2JI5O M1jvPVIyPzh|M{[5
human MOLT4 cells MoTBR5l1d3SxeHnjxsBie3OjeR?= MnTTO|IhcA>? NWe2eIIyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVU:OVESgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQSCwTR?= MnXmNlQ6ODB5MkW=
human LoVo cells Mn;wR5l1d3SxeHnjxsBie3OjeR?= M2jNe|czKGh? Mo\mR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUI9XdyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKwJI5O Mn\4NlA{PzFzOEO=
RPM18402 tumor cell line NIXCellRem:uaX\ldoF1cW:wIHHzd4F6 NY\2c5VPS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JINmdGxicILvcIln\XKjdHnvckBqdiCUUF2xPFQxOiC2dX3vdkBk\WyuIHzpcoUtKEmFNUC9NlAhdk1? MWOxOVQ5Ojl{OR?=
human SN12C cells NUixWWF3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTUlEzSyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEeLNUC9NlAhdk1? MYWyOVkxQTJ5OR?=
human RPMI8226 cells M2rDc2N6fG:2b4jpZ:Kh[XO|YYm= NFjtVZJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTWE2LOEKyOkBk\WyuczDifUBOXFRibXX0bI9lNCCLQ{WwQVIyKG6P NE\aVVgyQTBzMkm5Oi=>
HT-29 cells MYTDfZRwfG:6aXRCpIF{e2G7 M1rTU2N6fG:2b4jpZ4l1gSC5YYOg[IV1\XKvaX7l[EBqdiC4aYTyc{BqdiCKVD2yPUBk\Wyuczjjc4xwdilib3[gbJVu[W5idIXtc5Ih[2WubDDsbY5meyCkeTD1d4lv\yCPVGSgZZN{[XluIFnDOVA:OjVibl2= MVm3PFU{OzNz
SK-BR-3 cells NYLZXXBYS3m2b4TvfIlkyqCjc4PhfS=> Ml[0TY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmViKGPLMWJTNTNrLDDJR|UxRTJ4IH7N MW[xNVM{PDV4OR?=
HeLa cells NWfMTYZoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUDQcpB1T3Kxd4ToJIlvcGmkaYTpc44hd2ZiSHXMZUBk\WyuczDh[pRmeiB2IHThfZMtKEeLNUC9N|Ahdk1? MnPxNVc1OTh3OEK=
UACC 62 cells M1;lTmN6fG:2b4jpZ:Kh[XO|YYm= MnqzWIV{fGWmIHnuJJZqfHKxIH\vdkBkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB1fW2xcjDj[YxtKGyrbnWgWWFESyB4MjCocYVt[W6xbXGpMEBIUTVyPUOwJI5O MUWxNFcyPDVyMh?=
human HCT116 cells NVXxNGk5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\mN2hWT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IHL5JIZqfmVvZH;z[UBoem:5dHigbY5pcWKrdHnvckBie3OjeTygS2k2OD1|MDDuUS=> MnLVNlU6ODl{N{m=
human lung cancer cell line (H128) MlG2R5l1d3SxeHnjxsBie3OjeR?= NXHSRpZrUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdHWwZzDjZY5k\XJiY3XscEBtcW6nIDjINVI5MSxiSVO1NF0{OSCwTR?= MX:xNVM{PDV4OR?=
human MES-SA/Dx5 cells NGPZbJFEgXSxdH;4bYPDqGG|c3H5 M1ziUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1GWy2VQT;EfFUh[2WubIOgc5ZmemW6cILld5NqdmdiTVTSNUBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;M{Ogcm0> NWWyblZrOjF|NEG2O|Q>
human A2780 cells Mn3WR5l1d3SxeHnjxsBie3OjeR?= M4L1clczKGh? MmnyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zN{BvVQ>? MVGyOFkxODd{NR?=
human MDA-MB-435 cells MXXDfZRwfG:6aXRCpIF{e2G7 MoKyO|IhcA>? Ml7IR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFfJOVA:OzRibl2= NULtdlR4OjJ6NkewNVk>
human stomach cancer cell line (MKN45) MmmxR5l1d3SxeHnjxsBie3OjeR?= NV21R5p6UW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4he3SxbXHjbEBk[W6lZYKgZ4VtdCCuaX7lJEhOU052NTmsJGlEPTB;M{igcm0> NInCdZMyOTN|NEW2PS=>
human KB3-1 cells M{LhfWN6fG:2b4jpZ:Kh[XO|YYm= Ml23R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2I{NTFiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUSwJI5O MmfzNVg5Ojl|M{S=
mouse P388 cells NYn2R4c6S3m2b4TvfIlkyqCjc4PhfS=> NXTPUFF1S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhWDN6ODDj[YxteyCjZoTldkBOXFRiYYPzZZktKEmFNUC9OFUhdk1? NWn2cnVtOTh6MkmzN|Q>
A427 human lung carcinoma MUTQdo9tcW[ncnH0bY9vKGG|c3H5 M1vP[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JI1m[XO3cnXkJIFo[Wmwc4SgRVQzPyCqdX3hckBtfW6pIHPhdoNqdm:vYTygTWM2OD12OTDuUS=> NICzSWc6QDd4MUGx
human U251 cells NGm5NpNHfW6ldHnvckBie3OjeR?= NYXEc4x4Pi1{NDDo MXLJcohq[mm2aX;uJI9nKHSxcH;pd49u\XKjc3WtNUBqdiCqdX3hckBWOjVzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcHmyb4jpZU1qdmS3Y3XkJGhKTi1zYXzwbIEh[WOldX31cIF1cW:wIHnuJI52[2ynYYKg[Zh1emGldDDh[pRmeiB4IITvJFI1KGi{czDifUBqdW23bn;icI91KGGwYXz5d4l{NCCHQ{WwQVU1KG6P MX:yNlMxPTZzMh?=
human colon cancer cell line (WiDr) NIrWVIhEgXSxdH;4bYPDqGG|c3H5 MUjJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubDDsbY5mKCiZaVTyLUwhUUN3ME21OkBvVQ>? Ml33NVE{OzR3Nkm=
human HepG2 cells M4HUUmN6fG:2b4jpZ:Kh[XO|YYm= M1HEd|Qh\GG7cx?= MlvxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOEBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME22NEBvVQ>? NFHUcZoyQDV3NEmwOi=>
human KB cells M1S0U2N6fG:2b4jpZ:Kh[XO|YYm= NV\ueZlrPzJiaB?= MmfhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KHO3bH\vdohw\GGvaX7lJGIh[2:ub4LpcYV1emmlIHHzd4F6NCCLQ{WwQVYzNjVibl2= NGHT[WgzPTByOES1Oi=>
human HCT8 cells NXHQT2hHS3m2b4TvfIlkyqCjc4PhfS=> Mn30N{Bl[Xm| Mn3sR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVQCClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUewJI5O NV32S|ViOjB|OUK1OFU>
human GBM2 cells Ml;qVJJwdGmoZYLheIlwdiCjc4PhfS=> M{GyXlczKGh? M3XUR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOTNizszN M1npbVI3OzV3NUOy
SK-MEL-2 cells M1LHTWN6fG:2b4jpZ:Kh[XO|YYm= M{LJclczKGh? Moq5R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOLUKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvOTRizszN NYCydYNHOjF|NEG2O|Q>
human IGROV1 cells MlWyR5l1d3SxeHnjxsBie3OjeR?= NE\3eHM4OiCq MofIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTWdTV1ZzIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME2wMlE3KM7:TR?= NVTUfWx5OjF|NEG2O|Q>
human U87 cells MWjQdo9tcW[ncnH0bY9vKGG|c3H5 NIjiVXI1QCCq NUL4TFR5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFch[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlE3KM7:TR?= MYCyOFgzPjhzOB?=
human HT1080 cells M3HtOXBzd2yrZnXyZZRqd25iYYPzZZk> MYO0PEBp MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwQVAvOThizszN MmjTNlY1ODh6MUW=
human Hep3B cells MnXwVJJwdGmoZYLheIlwdiCjc4PhfS=> M3XRV|czKGh? NWjSR5k6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZA{SiClZXzsd{Bi\nSncjC3NkBpenNiYomgXHRVKGG|c3H5MEBIUTVyPUCuNlIh|ryP MXOxPVc6Pjl3Nh?=
human GBM3 cells NYT3NoNJWHKxbHnm[ZJifGmxbjDhd5NigQ>? MmS3O|IhcA>? M1zoVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOzhizszN Mk\sNlY{PTV3M{K=
human KBVIN cells NHz0dY1EgXSxdH;4bYPDqGG|c3H5 MYq3NkBp NWHwTIpTS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0KYSV6gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjRizszN NVHRXJFDOjV2M{i3Olk>
human GBM1 cells NFXqfGpRem:uaX\ldoF1cW:wIHHzd4F6 NFLYSIg4OiCq NYXReVJrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0yKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:OC52NjFOwG0> MUWyOlM2PTV|Mh?=
human U937 cells NGnpfZlEgXSxdH;4bYPDqGG|c3H5 NELlb3Q1QCCq MkfDR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{BmgHC{ZYPzbY5oKHB3MzDteZRidnRiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSExvJygTWM2OD1zLkGg{txO NFzhfJIyQTdzNUOxPS=>

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 92 mg/mL (200.91 mM)
Water 92 mg/mL (200.91 mM)
Ethanol <1 mg/mL
In vivo Saline 30 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
in solvent
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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  • Computed Result

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID