Topotecan HCl

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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In DMSO USD 230 In stock
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USD 320 In stock
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2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

    Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NGL6[ohEgXSxdH;4bYPDqGG|c3H5 NX7IVoZrPzJiaB?= NGPjXJBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJIlkPTB;MTDuUS=> NW\nOVc6OjB|N{GxPFM>
human Bel7402 cells NEXaOWxEgXSxdH;4bYPDqGG|c3H5 MmHCPVYhcA>? MnjRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRoVtPzRyMjDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvOiCwTR?= NGXiSIkyPTF4NUG0OC=>
human HCT8 cells MW\DfZRwfG:6aXRCpIF{e2G7 MVO5OkBp M4r3N2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDhiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xMlUhdk1? M4rqTVE2OTZ3MUS0
human MCF7 cells Mn32R5l1d3SxeHnjxsBie3OjeR?= MmjEPVYhcA>? NVK3OlNWS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvQCCwTR?= NVqwUpZrOTVzNkWxOFQ>
human A549 cells NXnMXmlRS3m2b4TvfIlkyqCjc4PhfS=> M1r2Nlk3KGh? NI[2NJBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9N{4zKG6P M1X0RVE2OTZ3MUS0
human colon carcinoma SW620 cell NVP2TVRsS3m2b4TvfIlkyqCjc4PhfS=> MoW1R5l1d3SxeHnjJJBwfGWwdHnheIlwdiCxZjDUc5BwfGWlYX6gLHRRMSCkeTD0bIUh[2:vcH;1coQhcW5iaIXtZY4h[2:ub36gZ4Fz[2mwb33hJHNYPjJyIHPlcIwhdGmwZTygTWM2OD1|LkKgcm0> M4nHNVEzPDB6N{C3
human MESSA cells NYDkNIZkS3m2b4TvfIlkyqCjc4PhfS=> M1zSc|czKGh? M4DRWmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1GW1ODIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME20JI5O Mn2xNlE{PDF4N{S=
human BGC823 cells NFXCSnlEgXSxdH;4bYPDqGG|c3H5 NWfqS3lOQTZiaB?= MojiR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ|IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE4{KG6P MVmxOVE3PTF2NB?=
human Ketr3 cells NEHmPGVEgXSxdH;4bYPDqGG|c3H5 NHXaVG86PiCq NFvEPHlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBM\XS{MzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQvQSCwTR?= NXPrSWRYOTVzNkWxOFQ>
LOX cells Mk\2R5l1d3SxeHnjxsBie3OjeR?= NIXCVpJEgXSxdH;4bYNqfHlid3HzJIRmfGW{bXnu[YQhcW5idnn0do8hcW5iTF;YJINmdGy|IDjt[Yxidm:vYTmgc4YhcHWvYX6geJVud3JiY3XscEBtcW6nczDifUB2e2mwZzDNWHQh[XO|YYmsJGlEPTB;NTDuUS=> NHXuZZc4QDV|M{Ox
human A549 cells NHPKb45HfW6ldHnvckBie3OjeR?= MYW0JIg> MYDBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF02KG6P MoHFNVgzODd5NEi=
human H69 cells MXnDfZRwfG:6aXRCpIF{e2G7 MVO3NkBp NGrDPXpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPjliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPU[gcm0> MXyyNVM1OTZ5NB?=
human A2780 cells MX3DfZRwfG:6aXRCpIF{e2G7 NUD3PHVGPzJiaB?= NXS4eXlyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNib4\ldoV5eHKnc4Ppcoch[WyyaHG1ZoV1[TNiaX70[YdzcW5iYYPz[ZN{\WRiYYOgZ4VtdCC|dYL2bZZidCCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVYhdk1? M3fHZlIzQTV7MkS2
human LNCAP cells NIjD[WpEgXSxdH;4bYPDqGG|c3H5 Ml\KO|IhcA>? NYDnbI97S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVE6FQWCgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVkhdk1? NVP2WWZXOjF|NEG2O|Q>
human HOP62 cells NUnLbmpJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXmxUmttT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUE:SNkKgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N NIKyRlYzPTlyOUK3PS=>
human MCF7 cells NILHVmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIJ6KG[rdnWt[I9{\SCpcn;3eIghcW6qaXLpeIlwdiCjc4PhfUwhUUN3ME2xNEBvVQ>? MkfZNlU6ODl{N{m=
human UACC62 cells MnTnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWjHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDVRWNEPjJiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCLQ{WwQVExKG6P MVqyOVkxQTJ5OR?=
human DU145 cells NYTpdmNOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4G1Xmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGRWOTR3IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= NF3teVczPTlyOUK3PS=>
LoVo cell line Mn3NR5l1d3SxeHnjxsBie3OjeR?= M4TBOGN6fG:2b4jpZ{Bxd3SnboTpZZRqd25ib3[gWI9xd3SnY3HuJEhVWCliYomgeIhmKGOxbYDveY5lKGmwIDjoeY1idiClb3zvdoVkfGGuIHPhcoNmeiCOb2\vJINmdGxibHnu[UwhUUN3ME2xNU43KG6P M4DnRVEzPDB6N{C3
human lymphoblast tumor cell line RPM18402 M3rzd2N6fG:2b4jpZ:Kh[XO|YYm= NXjnXppUS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdHmvcHjvZoxie3RidIXtc5Ih[2WubDDsbY5mKFKSTUG4OFAzNCCLQ{WwQVEzKG6P NGPZZYsyOjN7Mke0OS=>
human HL60 cells M3fQWWZ2dmO2aX;uJIF{e2G7 NEX2e|RCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIJ6KFOUQjDt[ZRpd2RuIFnDOVA:OTJibl2= MmjhNVk2PDF2OEO=
human A375 cells MoTyR5l1d3SxeHnjxsBie3OjeR?= M3\sdFczKGh? NHvtNVJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOzd3IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME2xN{BvVQ>? MViyNVM1OTZ5NB?=
COR-L23/P cell NGPlXFBEgXSxdH;4bYPDqGG|c3H5 MYHDfZRwfG:6aXPpeJkhdWWjc4Xy[YQhfXOrbnegeIhmKEORUj3MNlMheGG{ZX70ZYwhMEORUj3MNlMwWCliaIXtZY4hdm:wIIPtZYxtKGOnbHygcJVv\yClYYLjbY5wdWFiY3XscEBtcW6nLDDJR|UxRTF|LkKgcm0> NV[4NJg2OTF6ME[3NlQ>
human NCI-H460 cells NXT3VYVHWHKxbHnm[ZJifGmxbjDhd5NigQ>? MYW3NkBp NVzrRZZZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2:3bITldkBkd3WwdHXyJIFv[Wy7c3nzMEBKSzVyPUG2JI5O NGrq[4YzOTd6M{O2PS=>
human MOLT4 cells MmP2R5l1d3SxeHnjxsBie3OjeR?= MnjxO|IhcA>? MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG5JI5O M3;UT|I1QTByN{K1
human LoVo cells NFHDWVdEgXSxdH;4bYPDqGG|c3H5 M2XlblczKGh? NHziWpNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNd1[xIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlAhdk1? M2PLWFIxOzdzMUiz
RPM18402 tumor cell line NGLodItRem:uaX\ldoF1cW:wIHHzd4F6 M4nYNGNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDDj[YxtKHC{b3zp[oVz[XSrb36gbY4hWlCPMUi0NFIhfHWvb4KgZ4VtdCCuaX7lMEBKSzVyPUKwJI5O MVWxOVQ5Ojl{OR?=
human SN12C cells M{HFfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkDzS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV24yOkNiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCJSUWwQVIxKG6P MYCyOVkxQTJ5OR?=
human RPMI8226 cells MWDDfZRwfG:6aXRCpIF{e2G7 NECzfWlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTWE2LOEKyOkBk\WyuczDifUBOXFRibXX0bI9lNCCLQ{WwQVIyKG6P NGL2bI8yQTBzMkm5Oi=>
HT-29 cells NY\ifJBqS3m2b4TvfIlkyqCjc4PhfS=> NID0W3pEgXSxdH;4bYNqfHlid3HzJIRmfGW{bXnu[YQhcW5idnn0do8hcW5iSGStNlkh[2WubIOoZ49td25rIH;mJIh2dWGwIIT1cY9zKGOnbHygcIlv\XNiYomgeZNqdmdiTWTUJIF{e2G7LDDJR|UxRTJ3IH7N M1TCTVc5PTN|M{G=
SK-BR-3 cells MUDDfZRwfG:6aXRCpIF{e2G7 Mn[yTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmViKGPLMWJTNTNrLDDJR|UxRTJ4IH7N NEXETWkyOTN|NEW2PS=>
HeLa cells MkHPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEL0VpFIem:5dHigbY5pcWKrdHnvckBw\iCKZVzhJINmdGy|IHHmeIVzKDRiZHH5d{whT0l3ME2zNEBvVQ>? MojxNVc1OTh3OEK=
UACC 62 cells MYLDfZRwfG:6aXRCpIF{e2G7 M322fXRme3SnZDDpckB3cXS{bzDmc5Ih[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hfHWvb4KgZ4VtdCCuaX7lJHVCS0NiNkKgLI1mdGGwb33hLUwhT0l3ME2zNEBvVQ>? M4PiRVExPzF2NUCy
human HCT116 cells M3vWZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2jNRWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhEXDFzNjDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFfJOVA:OzBibl2= NGnnSIEzPTlyOUK3PS=>
human lung cancer cell line (H128) NGrhO3hEgXSxdH;4bYPDqGG|c3H5 M{XFbmlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx2dmdiY3HuZ4VzKGOnbHygcIlv\SBqSEGyPEktKEmFNUC9N|Ehdk1? NH\YUpYyOTN|NEW2PS=>
human MES-SA/Dx5 cells NHvOOFREgXSxdH;4bYPDqGG|c3H5 NEnhWHlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVNvU1GvSJg2KGOnbHzzJI93\XKneIDy[ZN{cW6pIF3EVlEh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyPUOzJI5O MWeyNVM1OTZ5NB?=
human A2780 cells NXm4blhwS3m2b4TvfIlkyqCjc4PhfS=> NGnJV244OiCq MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUOzJI5O M1HXVlI1QTByN{K1
human MDA-MB-435 cells NXu1S4plS3m2b4TvfIlkyqCjc4PhfS=> MV:3NkBp MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHnuZ5Vj[XSnZDDmc5IhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NF0{PCCwTR?= MYOyNlg3PzBzOR?=
human stomach cancer cell line (MKN45) NETrdoxEgXSxdH;4bYPDqGG|c3H5 NV;ybJNGUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4he3SxbXHjbEBk[W6lZYKgZ4VtdCCuaX7lJEhOU052NTmsJGlEPTB;M{igcm0> M1LpOVEyOzN2NU[5
human KB3-1 cells MUPDfZRwfG:6aXRCpIF{e2G7 NGrFcHhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSjNvMTDj[YxteyCjZoTldkBOXFRiYYPzZZktKEmFNUC9OFAhdk1? M4DlVlE5QDJ7M{O0
mouse P388 cells NXexRmtvS3m2b4TvfIlkyqCjc4PhfS=> NYDDfIVxS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhWDN6ODDj[YxteyCjZoTldkBOXFRiYYPzZZktKEmFNUC9OFUhdk1? MXKxPFgzQTN|NB?=
A427 human lung carcinoma M3\LfHBzd2yrZnXyZZRqd25iYYPzZZk> NX;vOWlWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhdWWjc4Xy[YQh[WejaX7zeEBCPDJ5IHj1cYFvKGy3bnegZ4Fz[2mwb33hMEBKSzVyPUS5JI5O MkPxPVg4PjFzMR?=
human U251 cells MnrpSpVv[3Srb36gZZN{[Xl? MUS2MVI1KGh? NFnNPJBKdmirYnn0bY9vKG:oIITvdI9qe2:vZYLhd4UuOSCrbjDoeY1idiCXMkWxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiaInwc5hq[S2rbnT1Z4VlKEiLRj2xZYxxcGFiYXPjeY12dGG2aX;uJIlvKG63Y3zlZZIh\Xi2cnHjeEBi\nSncjC2JJRwKDJ2IHjyd{BjgSCrbX31co9jdG:2IHHuZYx6e2m|LDDFR|UxRTV2IH7N M2nV[lIzOzB3NkGy
human colon cancer cell line (WiDr) MVXDfZRwfG:6aXRCpIF{e2G7 NXK4SndzUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIwhdGmwZTCoW4lFeiluIFnDOVA:PTZibl2= MkXkNVE{OzR3Nkm=
human HepG2 cells MUfDfZRwfG:6aXRCpIF{e2G7 MlzvOEBl[Xm| NVnnUms1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KE2WVDDhd5NigSxiSVO1NF03OCCwTR?= M4PGPVE5PTV2OUC2
human KB cells MmTNR5l1d3SxeHnjxsBie3OjeR?= NVSzSFJ{PzJiaB?= MnTxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KHO3bH\vdohw\GGvaX7lJGIh[2:ub4LpcYV1emmlIHHzd4F6NCCLQ{WwQVYzNjVibl2= MnXQNlUxODh2NU[=
human HCT8 cells NIXiVXNEgXSxdH;4bYPDqGG|c3H5 NWfYcXd7OyCmYYnz MWDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3Q5KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;N{Cgcm0> M1u4flIxOzl{NUS1
human GBM2 cells M4DsVXBzd2yrZnXyZZRqd25iYYPzZZk> M3XnRVczKGh? NUL6epFnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0zKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:OC5zMzFOwG0> NXvLSnRMOjZ|NUW1N|I>
SK-MEL-2 cells MlHSR5l1d3SxeHnjxsBie3OjeR?= NEjTNYY4OiCq MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMjDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NE4yPCEQvF2= NH\xSVgzOTN2MU[3OC=>
human IGROV1 cells NH76SVNEgXSxdH;4bYPDqGG|c3H5 NYizOVc2PzJiaB?= MnWwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTWdTV1ZzIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME2wMlE3KM7:TR?= NEfDUWYzOTN2MU[3OC=>
human U87 cells MXTQdo9tcW[ncnH0bY9vKGG|c3H5 NGHoSXk1QCCq MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMU[g{txO MortNlQ5OjZ6MUi=
human HT1080 cells MYrQdo9tcW[ncnH0bY9vKGG|c3H5 MUO0PEBp MWDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwQVAvOThizszN Mlr6NlY1ODh6MUW=
human Hep3B cells NYTOUmVIWHKxbHnm[ZJifGmxbjDhd5NigQ>? NITlNII4OiCq NFz3UmJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldFNDKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTB;MD6yNkDPxE1? MXuxPVc6Pjl3Nh?=
human GBM3 cells MoD1VJJwdGmoZYLheIlwdiCjc4PhfS=> M{m3eVczKGh? NX7nd4RNSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0{KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:OC5|ODFOwG0> MWSyOlM2PTV|Mh?=
human KBVIN cells NF;HPHhEgXSxdH;4bYPDqGG|c3H5 Mli5O|IhcA>? Mn7UR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2JXUU5iY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlQh|ryP NFrEZ5YzPTR|OEe2PS=>
human GBM1 cells M2D4W3Bzd2yrZnXyZZRqd25iYYPzZZk> NFna[5k4OiCq M{nZS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvPDZizszN NHHGPGUzPjN3NUWzNi=>
human U937 cells M3fJXWN6fG:2b4jpZ:Kh[XO|YYm= Mln6OFghcA>? NUjldXJ1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTl|NzDj[YxteyCneIDy[ZN{cW6pIIC1N{BufXSjboSgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTFvwKwhUUN3ME2xMlEh|ryP MWexPVcyPTNzOR?=

... Click to View More Cell Line Experimental Data

In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Formulation: PBS
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 92 mg/mL (200.91 mM)
Water 92 mg/mL (200.91 mM)
Ethanol slightly soluble or insoluble
In vivo Add solvents individually and in order:
Saline
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
in solvent
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00553189 Completed Solid Tumors|Lymphomas National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 9, 2007 Phase 1
NCT00117013 Completed Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) June 28, 2005 Phase 1
NCT02980809 Not yet recruiting Small Cell Lung Cancer First Hospitals affiliated to the China PLA General Hospital March 2017 Phase 2
NCT02963090 Not yet recruiting Small Cell Lung Cancer Alliance Foundation Trials, LLC.|Merck Sharp & Dohme Corp. December 2016 Phase 2
NCT02348398 Withdrawn Cervical Cancer M.D. Anderson Cancer Center|GlaxoSmithKline August 2016 Phase 2
NCT02822157 Not yet recruiting Ovarian Epithelial Cancer Universitaire Ziekenhuizen Leuven|AstraZeneca August 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID