Ibuprofen

Catalog No.S1638

Ibuprofen (Dolgesic) is an anti-inflammatory inhibitor targeting COX-1 and COX-2 with IC50 of 13 μM and 370 μM, respectively.

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In DMSO USD 130 In stock
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Ibuprofen  Chemical Structure

Ibuprofen Chemical Structure
Molecular Weight: 206.28

Validation & Quality Control

Quality Control & MSDS

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Product Description

Biological Activity

Description Ibuprofen (Dolgesic) is an anti-inflammatory inhibitor targeting COX-1 and COX-2 with IC50 of 13 μM and 370 μM, respectively.
Targets COX-1 COX-2
IC50 13 μM [1] 370 μM [1]
In vitro Ibuprofen works by inhibiting the enzyme cyclooxygenase COX-1 and COX-2, which convert arachidonic acid to prostaglandin H2 (PGH2). Its action is similar to aspirin, indomethacin and all other NSAIDs in intact cells, broken cells, and purified enzyme preparations. [1] Ibuprofen inhibits the constitutive activation of NF-κB and IKKα in the androgen-independent prostate tumor cells PC-3 and DU-145. It sensitizes prostate cells to ionizing radiation and blocks stimulated activation of NF-κB following exposure to TNFα or ionizing radiation in the androgen-sensitive prostate tumor cell line LNCaP. Both of these cannot be attributed directly to inhibition of IκB-α kinase but to inhibition of an upstream regulator of IKKα. [2] Ibuprofen exerts an anticancer effect by reducing survival of cancer cells. Ibuprofen is more efficacious than aspirin and acetaminophen, and comparable with (R)-flurbiprofen and indomethacin in induction of p75NTR protein (a tumor and metastasis suppressor) expression in cell lines from bladder and other organs. [3]
In vivo Ibuprofen reacts with the heme group of cyclooxygenase to prevent arachidonic acid conversion. Prior exposure to Ibuprofen in vivo protects cyclooxygenase completely from the irreversible effects of aspirin in platelets. [4] Ibuprofen treatment is effective in attenuating joint inflammation and early articular cartilage degeneration in the adult female Sprague-Dawley rat model induced by high-repetition and high-force (HRHF) task. It dose this by blocking the increases in serum C1 and 2C (a biomarker of collagen I and II degradation) as well as the ratio of collagen degradation to synthesis (C1, 2C/CPII, the latter a biomarker of collage type II synthesis) induced by HRHF. [5]
Features Considered a core medicine in the WHO's "WHO Model List of Essential Medicines" (a list of the minimum medical requirements for a basic healthcare system).

Protocol(Only for Reference)

Kinase Assay: [1]

Radiochemical enzyme assays for COX-1 and COX-2 10 μL of purified COX-1 (0.7-0.8 μg) or COX-2 (3.0 units, 0.3μg) is activated with 50 μL of cofactor solution [l-epinephrine (1.3 mg/mL), reduced glutathione (0.3 mg/mL), and hematin (1.3 mg/mL) in oxygen-free Tris-HCl buffer (pH 8.0)]. The enzyme solution (60 μL) is added to Ibuprofen solutions or DMSO (20 μL) after [14C]arachidonic acid is added in 0.2 mL eight-strip test tubes and preincubated 10 minutes on ice. Samples are incubated for 15 minutes at 37 °C, after which the reaction is terminated by addition of 10 μL of 2 M HCl and 5 μL of carrier solution (PGE2 and PGF2α, 0.2 μg/mL of each in EtOH). The unmetabolized arachidonic acid is separated from the prostaglandin products by column chromatography and eluted with n-hexane-dioxane-glacial acetic acid (70:30:1). The prostaglandin products are then eluted with EtOAc-MeOH (85:15), and the samples are counted in a Packard scintillation spectrometer. IC50 values are obtained by linear regression analysis.

Cell Assay: [3]

Cell lines Bladder epithelial cell line T24, RT-4 transitional cell papilloma bladder cell line, 5637 primary carcinoma bladder cell line, HCT-116, MDAMB231, MCF7, HEK293, A549, SKOV3 and DU145
Concentrations Dissolved in DMSO at a concentration of 200 mM for making the stock solution, final concentration ~2 mM
Incubation Time 48 hours
Method Each cell line is incubated with Ibuprofen of various concentrations for 48 hours. Cell survival is estimated by the MTT assay, and cell death is determined by Hoechst staining used to distinguish between intact cell nuclei and fragmented nuclei undergoing cell death. Cells are lysed and analyzed by western blotting for detection of the p75NTR protein.

Animal Study: [5]

Animal Models Female Sprague-Dawley rats with joint inflammation induced by high-repetition and high-force (HRHF) tasks
Formulation Dissolved in DMSO and diluted in saline
Dosages 45 mg/kg
Administration Taken orally every day
Solubility 1% DMSO/30% polyethylene glycol/1% Tween 80, , 20 mg/mL
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
1

References

[1] Noreen Y, et al. J Nat Prod, 1998, 61(1), 2-7.

[2] Palayoor ST, et al. Oncogene, 1999, 18(51), 7389-7394.

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Clinical Trial Information( data from http://clinicaltrials.gov)

NCT Number Recruitment Conditions Sponsor
/Collaborators
Start Date Phases
NCT02128191 Not yet recruiting Bronchopulmonary Dysplasia Samsung Medical Center 2014-05 Phase 2
NCT01951105 Not yet recruiting Sub-acute Back Pain Northwestern University|National Institutes of Health (NIH)|National Institute of Dental and Craniofacial Research (NIDCR) 2014-05 Phase 4
NCT02013427 Not yet recruiting Chronic Back Pain Northwestern University|National Center for Complementary and Alternative Medicine (NCCAM)|National Institutes of Health (NIH) 2014-05 Phase 4
NCT02116790 Not yet recruiting Acute Pain Northwestern University|National Institute of Dental and Craniofacial Research (NIDCR)|National Institutes of Health (NIH) 2014-05 Phase 2
NCT02084667 Not yet recruiting Endometriosis Mount Sinai Hospital, Canada 2014-06

Chemical Information

Download Ibuprofen SDF
Molecular Weight (MW) 206.28
Formula

C13H18O2

CAS No. 15687-27-1
Storage 3 years -20℃Powder
6 months-80℃in DMSO
Synonyms
Solubility (25°C) * In vitro DMSO 41 mg/mL (198 mM)
Water <1 mg/mL (<1 mM)
Ethanol 41 mg/mL (198 mM)
In vivo 1% DMSO/30% polyethylene glycol/1% Tween 80, 20 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

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