Ponatinib (AP24534)

Catalog No.S1490

Ponatinib (AP24534) Chemical Structure

Molecular Weight(MW): 532.56

Ponatinib (AP24534) is a novel, potent multi-target inhibitor of Abl, PDGFRα, VEGFR2, FGFR1 and Src with IC50 of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM and 5.4 nM in cell-free assays, respectively.

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  •  

    RCH-ACV cells were treated with ponatinib(50 nM), PCI-32765(50 nM), or BMS-599626(500 nM) over a time course, and whole-cell extracts were subjected to immunoblot analysis for total or phospho-AKT.
     

    Cancer Cell 2012 22(5), 656-667 . Ponatinib (AP24534) purchased from Selleck.

    Proliferation curves of established human (A), novel human (B), and murine(C) IDHm (red) and WT (black) ICC lines and MMNK-1 cells treated with increasing doses of the TKIs dasatinib, saracatinib, bosutinib, and ponatinib.

    Cancer Discov, 2016, 6(7):727-39.. Ponatinib (AP24534) purchased from Selleck.

  • The KatoIII diffuse gastric cancer cell line was treated with FGFR2 inhibitors of varying specificity. The Y-axis depicts percent survival versus the X-axis with log concentrations.

    Genome Biol, 2014, 15(8): 428 . Ponatinib (AP24534) purchased from Selleck.

    Western blot analysis of LC-2/ad cells treated with increasing doses of ponatinib for 2 hours. Lysates were analyzed via western blotting with the antibodies indicated, with P-TYR used to assess P-RET

    Mol Cancer Ther, 2017. Ponatinib (AP24534) purchased from Selleck.

Purity & Quality Control

Choose Selective Bcr-Abl Inhibitors

Biological Activity

Description Ponatinib (AP24534) is a novel, potent multi-target inhibitor of Abl, PDGFRα, VEGFR2, FGFR1 and Src with IC50 of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM and 5.4 nM in cell-free assays, respectively.
Targets
Abl [1]
(Cell-free assay)
PDGFRα [1]
(Cell-free assay)
VEGFR2 [1]
(Cell-free assay)
FGFR1 [1]
(Cell-free assay)
c-Src [1]
(Cell-free assay)
0.37 nM 1.1 nM 1.5 nM 2.2 nM 5.4 nM
In vitro

AP24534 potently inhibits native Abl, AblT315I, and other clinically important Abl kinase domain mutants with IC50 of 0.30 nM–2 nM. AP24534 doesn't inhibit Aurora kinase family members, nor does it inhibit insulin receptor or CDK2/cyclin E. AP24534 inhibits proliferation of Ba/F3 cells expressing Bcr-Abl with IC50 of 0.5 nM, as well as Ba/F3 cells expressing a range of Bcr-Abl mutants with IC50 of 0.5 nM–36 nM. The inhibition of proliferation by AP24534 is correlated with induction of apoptosis. [1-2] In leukemic cell lines containing activated forms of FLT3, KIT, FGFR1, and PDGFRα receptors, AP24534 potently inhibits receptor phosphorylation and cellular proliferation with IC50 of 0.3 nM to 20 nM. In MV4-11 (FLT3-ITD(+/+)) but not RS4;11 (FLT3-ITD(–/–)) AML cells, AP24534 inhibits FLT3 signaling and induced apoptosis at less than 10 nM. AP24534 inhibits viability of primary leukemic blasts from a FLT3-ITD positive AML patient with IC50 of 4 nM but not those from patients with AML expressing native FLT3. [3] In Ba/F3 cells engineered to express activated FGFR1-4, AP24534 potently inhibits FGFR-mediated signaling and viability with IC50 lower than 40 nM. In cell lines representing multiple tumor types (endometrial, bladder, gastric, breast, lung, and colon), and containing FGFRs dysregulated by a variety of mechanisms, AP24534 inhibits FGFR-mediated signaling with IC50 less than 40 nM and inhibits cell growth with IC50 of 7 nM–181 nM. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
EoL-1-cell MmnlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojFTWM2OD1yLkCwNFAxODB|NUmg{txO MnLuV2FPT0WU
MV-4-11 NXzRS5I1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\ON2k1UUN3ME2wMlAxODB7NU[g{txO MUDTRW5ITVJ?
MEG-01 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTBwMECwNVI5KM7:TR?= NUezUHA6W0GQR1XS
LAMA-84 NV\ieIRKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVP4UIpFUUN3ME2wMlAxODF4NTFOwG0> NEjObJhUSU6JRWK=
CGTH-W-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzITWM2OD1yLkCwNVY6KM7:TR?= NEWwZlVUSU6JRWK=
KASUMI-1 NIriTYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPZXXRCUUN3ME2wMlAxOjF5IN88US=> MYfTRW5ITVJ?
BE-13 NYOzN2J6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmToTWM2OD1yLkCwNlU6KM7:TR?= NGe4RmVUSU6JRWK=
CTV-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnWwTWM2OD1yLkCwOVIyKM7:TR?= NGPxT5lUSU6JRWK=
NKM-1 MmfwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDObVJKSzVyPUCuNFA3PTVizszN NXXE[ohbW0GQR1XS
MONO-MAC-6 M3PCXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2G4T2lEPTB;MD6wNFg{PyEQvF2= M3;kTnNCVkeHUh?=
A204 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTBwMEGyNVgh|ryP NIjEb|FUSU6JRWK=
GDM-1 NF[1[4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnmWJZKSzVyPUCuNFE5OzNizszN MlntV2FPT0WU
AN3-CA Mm\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTBwMEG4O|Mh|ryP M2jlWnNCVkeHUh?=
BL-70 M2rDUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2T0XGlEPTB;MD6wNlE6QSEQvF2= NEXXeZVUSU6JRWK=
Daudi MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTBwMEK1N|ch|ryP NXf5eFhXW0GQR1XS
KU812 NV7r[4ZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7HZWZyUUN3ME2wMlAzQDR6IN88US=> M1\rbHNCVkeHUh?=
KG-1 NIHRWZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTBwMEOyPVQh|ryP NFz1UodUSU6JRWK=
DOHH-2 NXO0dYJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;QWGRKSzVyPUCuNFQ5ODNizszN NWjJe29oW0GQR1XS
HuO9 NGrtWpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Dpd2lEPTB;MD6wOVQ1QCEQvF2= NF\oRnpUSU6JRWK=
EB2 Mni4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHZPXhKSzVyPUCuNFU2PzZizszN M{fBSXNCVkeHUh?=
G-402 MlXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVe0fmFDUUN3ME2wMlA3OzRzIN88US=> NVHrV5dNW0GQR1XS
SU-DHL-1 M2DSTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTBwME[zO|kh|ryP M2\0S3NCVkeHUh?=
NCI-H719 NHHGdGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;IT2lEPTB;MD6wO|g5QCEQvF2= M4nhNHNCVkeHUh?=
MLMA NVjpe5VmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlz3TWM2OD1yLkC5NFYh|ryP MWPTRW5ITVJ?
KM12 NFy1N|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfabnFKSzVyPUCuNFk4OzdizszN MWHTRW5ITVJ?
SUP-T1 NYi5VG0zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVq5UWNrUUN3ME2wMlA6QTh{IN88US=> NGr3bIVUSU6JRWK=
MZ1-PC M1Liemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmP5TWM2OD1yLkGxN|M3KM7:TR?= NIDSeXVUSU6JRWK=
MFE-296 NG\GO49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTBwMUG4N|kh|ryP MnLOV2FPT0WU
ALL-PO MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwMUK0OFMh|ryP NEPnbllUSU6JRWK=
GB-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml34TWM2OD1yLkGyOVEzKM7:TR?= NVvwcGtDW0GQR1XS
SIG-M5 NVXRWZdDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTBwMUOzO{DPxE1? MoHjV2FPT0WU
P30-OHK M2PkbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDQbIpKSzVyPUCuNVU3PzJizszN M3PSVHNCVkeHUh?=
NCI-H716 M4S3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwMU[yOlYh|ryP M4X2S3NCVkeHUh?=
MPP-89 NY\BTlRoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTBwMU[5N|Mh|ryP M2S2W3NCVkeHUh?=
SF126 M4e2emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LxVGlEPTB;MD6xO|A3OyEQvF2= MkjWV2FPT0WU
CTB-1 NFH2fIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjaUGlUUUN3ME2wMlE4OzF4IN88US=> Mm\kV2FPT0WU
CA46 NYnpZ3I{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HVUWlEPTB;MD6xO|Q5QSEQvF2= MorBV2FPT0WU
NCI-H520 M1\sNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDOPWZKSzVyPUCuNVc4OzJizszN NIDLcHNUSU6JRWK=
CAL-85-1 NETj[nhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33PN2lEPTB;MD6xO|kzPyEQvF2= M2n6THNCVkeHUh?=
NCI-H2342 MlXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrRTWM2OD1yLkG4OVc4KM7:TR?= M{XjXnNCVkeHUh?=
697 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTBwMUi4O|Eh|ryP NHTE[|JUSU6JRWK=
NY NGXuVIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjxTWM2OD1yLkG5Nlg4KM7:TR?= NIPrclNUSU6JRWK=
T98G M13yWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTBwMUmzNVgh|ryP MVXTRW5ITVJ?
NCI-H1703 NHXMSoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPLTWM2OD1yLkG5OlE2KM7:TR?= MkW2V2FPT0WU
NCI-H2087 NXTQcJFGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTBwMkC0N|kh|ryP NUnYS5hZW0GQR1XS
PF-382 NXn3XW5jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7wOo5KSzVyPUCuNlA5PzNizszN NFLmbGJUSU6JRWK=
HCT-116 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fDWGlEPTB;MD6yNVA3QSEQvF2= Ml3pV2FPT0WU
CAKI-1 NEXaNGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTBwMkG2OlYh|ryP NVHHOXlyW0GQR1XS
CCF-STTG1 M3KzVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDQfFZKSzVyPUCuNlI1PzVizszN MV;TRW5ITVJ?
D-392MG M2PBdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDoT|dKSzVyPUCuNlMyPzdizszN MYTTRW5ITVJ?
RT-112 NXznd41ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTBwMkO3PVUh|ryP MmDiV2FPT0WU
MES-SA M1e5WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTBwMkSxPFch|ryP M3;6[HNCVkeHUh?=
LC-2-ad MkLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\HSGlEPTB;MD6yOFI3PSEQvF2= NHvuTIFUSU6JRWK=
NOS-1 MkXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTBwMkSzOlch|ryP M37o[XNCVkeHUh?=
NALM-6 M{L6b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV24UlFSUUN3ME2wMlI2PTR|IN88US=> MmW5V2FPT0WU
TE-15 MlzVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfWN5hDUUN3ME2wMlI3ODlzIN88US=> NY\IfmhSW0GQR1XS
786-0 M1uzZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHTbG1YUUN3ME2wMlI3OjJ6IN88US=> NWTVdmVlW0GQR1XS
IST-MES1 M1TN[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMk[4Nlkh|ryP M2rzSXNCVkeHUh?=
KYSE-180 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7jTWM2OD1yLkK2PFkyKM7:TR?= MV;TRW5ITVJ?
YT NIjXUVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXTTWM2OD1yLkK2PVc1KM7:TR?= NEDL[nFUSU6JRWK=
A427 MmLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlK5TWM2OD1yLkK3NlY1KM7:TR?= M37YWXNCVkeHUh?=
KYSE-450 NHLMS2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HodGlEPTB;MD6yO|MzQSEQvF2= M3HJcXNCVkeHUh?=
HuH-7 NH7iRZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTBwMke3OlMh|ryP NUjqSJhbW0GQR1XS
CAS-1 M3TDS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDYTWM2OD1yLkK4N|A{KM7:TR?= MojqV2FPT0WU
KURAMOCHI MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTBwMki0NVkh|ryP NXXzXXZzW0GQR1XS
ACHN NWf3dmVbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jCUWlEPTB;MD6yPVMzOyEQvF2= NUnKdWxKW0GQR1XS
HGC-27 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTBwMkmzOVMh|ryP NETsRZRUSU6JRWK=
A172 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTBwMkm1O|ch|ryP M{\TV3NCVkeHUh?=
SNU-449 NHnJfZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwMkm5OlQh|ryP NHrhUY9USU6JRWK=
SW982 M{\HdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFj5elBKSzVyPUCuNlk6PzhizszN M1vKNHNCVkeHUh?=
EFO-21 M{LwWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHqyXYFKSzVyPUCuN|A1OTFizszN NW\LfXY6W0GQR1XS
NCI-H2030 NHfWWGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLiSlNKSzVyPUCuN|EyPjRizszN M4P4dHNCVkeHUh?=
HT-144 MmDSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInkRnVKSzVyPUCuN|EzQCEQvF2= MV;TRW5ITVJ?
MFE-280 NIn5TFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTBwM{G0OVMh|ryP NUTnWYtMW0GQR1XS
SK-LU-1 M3[2[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml36TWM2OD1yLkOxOFY3KM7:TR?= MXLTRW5ITVJ?
NCI-H2052 NWP2b|VDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnj6TWM2OD1yLkOxOFg1KM7:TR?= MVzTRW5ITVJ?
SK-HEP-1 NHyxWHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHLTWM2OD1yLkOzN|Y5KM7:TR?= NGHxZ5pUSU6JRWK=
QIMR-WIL MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnOemxKSzVyPUCuN|M1OjRizszN MmnyV2FPT0WU
A2780 M3LFVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3jPZ2lEPTB;MD6zOFMxPCEQvF2= M13WNHNCVkeHUh?=
ACN MoPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17xXWlEPTB;MD6zOFg4OyEQvF2= NUi4bHVtW0GQR1XS
BxPC-3 NETtTlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlznTWM2OD1yLkO0PVg6KM7:TR?= MoXvV2FPT0WU
BCPAP M3TqTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYi1eYVCUUN3ME2wMlM3ODBzIN88US=> M1zBO3NCVkeHUh?=
HOP-62 M{LqOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TRTGlEPTB;MD6zOlExPiEQvF2= MWDTRW5ITVJ?
SW1710 M3nFS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHVcYJKSzVyPUCuN|Y1PTVizszN MVvTRW5ITVJ?
OPM-2 M{DBT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvx[Y5FUUN3ME2wMlM3PDd|IN88US=> NXPxUGVbW0GQR1XS
DBTRG-05MG MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYm4Vm1CUUN3ME2wMlM5ODV2IN88US=> M3jDNnNCVkeHUh?=
SK-MEL-24 M3;LVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1j6bGlEPTB;MD6zPVc6OSEQvF2= NGT0dHVUSU6JRWK=
8305C MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;oWVdKSzVyPUCuN|k4QTNizszN NI\JUmVUSU6JRWK=
OVCAR-4 Mn22S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fCS2lEPTB;MD60NVU5PiEQvF2= MkXxV2FPT0WU
HLE Mn\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\vSY5KSzVyPUCuOFE5PiEQvF2= MUnTRW5ITVJ?
NCI-H747 NYroe2RlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjsOJVKSzVyPUCuOFIyOzdizszN NVLjPYVmW0GQR1XS
SW780 NF3q[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnGTWM2OD1yLkSyN|Q3KM7:TR?= NHvM[2JUSU6JRWK=
KYSE-150 NX\BVWdLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33LcmlEPTB;MD60NlQyPSEQvF2= M{XJdHNCVkeHUh?=
H4 M2L2Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PlbGlEPTB;MD60NlQ2OSEQvF2= NE[5bpJUSU6JRWK=
SW1088 NYPxbVJ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmiyTWM2OD1yLkS0N|kh|ryP M4DoR3NCVkeHUh?=
SF268 M4rGbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPDTWM2OD1yLkS0PFYh|ryP MXHTRW5ITVJ?
RT4 Mn\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NID6cWVKSzVyPUCuOFUyOTFizszN NVnKZ5ZVW0GQR1XS
NCI-H522 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzSTWM2OD1yLkS1Nlch|ryP M1r4T3NCVkeHUh?=
LCLC-97TM1 MnTGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HoNWlEPTB;MD60OlE6PSEQvF2= NWC2T3hpW0GQR1XS
CAL-39 NYD5WWU5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTBwNE[1NVkh|ryP MVTTRW5ITVJ?
CAL-120 M2LRd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnmTWM2OD1yLkS3OlQ{KM7:TR?= MnvmV2FPT0WU
ESS-1 M3\LSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2m4dGlEPTB;MD60O|gzOSEQvF2= NVG5XFg2W0GQR1XS
KARPAS-45 NYXFenpWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTBwNEi5N|Mh|ryP NED3fXRUSU6JRWK=
DU-4475 M3TmVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\nTWM2OD1yLkS5OVEyKM7:TR?= M{L1WHNCVkeHUh?=
VMRC-RCZ MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHUOYp3UUN3ME2wMlQ6QTB2IN88US=> NWDIRYdxW0GQR1XS
HOS Mo\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7KVplTUUN3ME2wMlUxOzZ3IN88US=> NInZcZJUSU6JRWK=
MOLT-16 NEHWSZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTBwNUG4NFgh|ryP NIjEdYJUSU6JRWK=
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OVCAR-5 NIrQO|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvaTWM2OD1zLkmwOlc2KM7:TR?= MX;TRW5ITVJ?
SNG-M M4m2UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDPV5FKSzVyPUGuPVU5PThizszN M2XtenNCVkeHUh?=
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L-428 M{fvbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHW3W2ZKSzVyPUGuPVkyPzlizszN MlLtV2FPT0WU
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COLO-678 M131cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml[wTWM2OD1{LkC0NlA4KM7:TR?= MUfTRW5ITVJ?
MEL-JUSO MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PqTmlEPTB;Mj6wO|g2OSEQvF2= MmHWV2FPT0WU
HCC38 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILGNpZKSzVyPUKuNFg1OzNizszN MlruV2FPT0WU
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OAW-42 MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUi5eIlTUUN3ME2yMlA6PzFzIN88US=> MVHTRW5ITVJ?
LB996-RCC MoPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXYNWRyUUN3ME2yMlE{QDF5IN88US=> MmHGV2FPT0WU
COR-L105 M3\CPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3KxWWlEPTB;Mj6xOlM4OyEQvF2= MXHTRW5ITVJ?
RCC10RGB NUfNNnFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\VTWM2OD1{LkG4OVc3KM7:TR?= NW\tOWVLW0GQR1XS
A431 M1\aNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HLVGlEPTB;Mj6xPVAyQSEQvF2= MXXTRW5ITVJ?
NCI-H292 NFrldYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTJwMkO5OVEh|ryP M3q3S3NCVkeHUh?=
SK-UT-1 NGX5S|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnv2TWM2OD1{LkOzNVg1KM7:TR?= NI[zeVJUSU6JRWK=
PANC-08-13 NF;ofGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYT3T4VnUUN3ME2yMlM{OjN3IN88US=> M{SwSHNCVkeHUh?=
ST486 NGi5fnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vBeWlEPTB;Mj60NVA1QCEQvF2= M{LIR3NCVkeHUh?=
SJSA-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LhSmlEPTB;Mj60NVI4OSEQvF2= MWHTRW5ITVJ?
RKO M{TwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jWc2lEPTB;Mj60NlAzQSEQvF2= NYrsdVZ{W0GQR1XS
NCI-H1793 NFXaN3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TMOmlEPTB;Mj60OFAzQSEQvF2= M1TxT3NCVkeHUh?=
639-V M1:0SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDncGtKSzVyPUKuOFU{QDJizszN MXXTRW5ITVJ?
ES3 MnK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1[xdmlEPTB;Mj60PVc{PyEQvF2= NXXRVZNvW0GQR1XS
NCI-H1792 NXnKeHZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jkTmlEPTB;Mj61OlgzPiEQvF2= Mn\nV2FPT0WU
NCI-H1651 Ml7xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPERVU4UUN3ME2yMlU5OjFizszN M3PHb3NCVkeHUh?=
SN12C M{TtU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkWyTWM2OD1{LkW4OFg4KM7:TR?= NYXvUHZvW0GQR1XS
COLO-679 NUW1W5lTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDjT|NKSzVyPUKuOVg3ODVizszN Mn;NV2FPT0WU
U-118-MG NVfEZm15T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33ieWlEPTB;Mj61PFg4OiEQvF2= MYTTRW5ITVJ?
Ramos-2G6-4C10 NYrlXW1DT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTJwNkS4NFch|ryP MmLVV2FPT0WU
HCT-15 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTJwNki3PVIh|ryP NX;mUllOW0GQR1XS
MDA-MB-157 M3jNemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFezS|FKSzVyPUKuO|A4PDdizszN NYPxNnI{W0GQR1XS
K5 NYr1SXpnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7K[I9KSzVyPUKuO|E5PDVizszN NVfFR|hiW0GQR1XS
MKN45 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHOT4VKSzVyPUKuO|M{PjZizszN MYLTRW5ITVJ?
DMS-273 NIruZ2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTJwN{W2N|Ih|ryP MnnqV2FPT0WU
C8166 Mn[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPNTWM2OD1{Lke4OVE{KM7:TR?= MWnTRW5ITVJ?
Calu-3 NFHwUW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV74d2RZUUN3ME2yMlc5QTZ7IN88US=> M{TBfnNCVkeHUh?=
SNB75 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTJwOEi1N|Uh|ryP NIPWSGRUSU6JRWK=
BFTC-905 M3TFS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rTNWlEPTB;Mj65NFE6OSEQvF2= NF7UN4tUSU6JRWK=
C-4-II Mn7HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\qZ3NKSzVyPUKuPVE{PTNizszN MVnTRW5ITVJ?
MS-1 NFLJUZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXBTWM2OD1{Lkm2O|Y6KM7:TR?= M2XvVHNCVkeHUh?=
A375 NHH3OZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGX2SXBKSzVyPUOuNFgyQDRizszN Mn\sV2FPT0WU
KYSE-70 NHH4d5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmmxTWM2OD1|LkC4PFgzKM7:TR?= NW[yUJptW0GQR1XS
KYSE-510 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PMR2lEPTB;Mz6xNFk6PSEQvF2= MoHYV2FPT0WU
LN-405 M{\kO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzYUZJKSzVyPUOuNVM2OThizszN NEHYc3dUSU6JRWK=
BOKU M33LW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfObFlKSzVyPUOuNVQzPTZizszN M1K5TnNCVkeHUh?=
TE-10 M{HjVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfKfYRPUUN3ME2zMlE4OzZ{IN88US=> NVzsdW5UW0GQR1XS
MKN1 MkHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TKZWlEPTB;Mz6xPFQ5OyEQvF2= Mk\NV2FPT0WU
M059J MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrmVG9KSzVyPUOuNlAyPzNizszN MnHQV2FPT0WU
GOTO NUjmfFJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTNwMkKwN|kh|ryP Ml;hV2FPT0WU
DK-MG MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkC5TWM2OD1|LkK1PVc3KM7:TR?= MWLTRW5ITVJ?
NCI-H727 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHmyWlZKSzVyPUOuNlY2QCEQvF2= NYLQe3FpW0GQR1XS
NB12 M{XKO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;CTWM2OD1|LkOzNlI1KM7:TR?= MWnTRW5ITVJ?
DOK MojJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\lV4pKSzVyPUOuN|YyQDZizszN NF7y[oVUSU6JRWK=
KYSE-410 M2LmRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvmWmtFUUN3ME2zMlM3Ojh5IN88US=> NUH1Zo55W0GQR1XS
AM-38 NVq3TY8{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHwW41KSzVyPUOuN|gxPTFizszN M4P1bHNCVkeHUh?=
GR-ST NYDRWoxLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTNwNEW3N|Ih|ryP MVfTRW5ITVJ?
D-247MG NHKzO3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjoTWM2OD1|LkS4NlY{KM7:TR?= NY\sXHJHW0GQR1XS
TE-8 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DYTWlEPTB;Mz60PVM{OyEQvF2= NGPOWIpUSU6JRWK=
LS-411N NYrJNoZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTNwNEm2PVIh|ryP MVPTRW5ITVJ?
SK-MES-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;GOZVKSzVyPUOuOVY1OzNizszN M1fZOnNCVkeHUh?=
SF295 NWTlNWlZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTNwNkG1OVIh|ryP MYjTRW5ITVJ?
SK-MEL-30 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXq2ZXlvUUN3ME2zMlY2QTJzIN88US=> MmfQV2FPT0WU
LB2241-RCC MkiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDaUnZKSzVyPUOuOlYzPiEQvF2= NG[2R|lUSU6JRWK=
PC-14 M{H3Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknRTWM2OD1|Lk[2OFM4KM7:TR?= M{fS[HNCVkeHUh?=
EW-7 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\xfFNKSzVyPUOuOlc3QTNizszN NHLPXmZUSU6JRWK=
NMC-G1 MnXQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[xTWM2OD1|LkexPFM4KM7:TR?= MVTTRW5ITVJ?
TE-441-T MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnLbFdmUUN3ME2zMlc{ODJ6IN88US=> MV;TRW5ITVJ?
NCI-H1618 NV7DZoc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfmS4NKSzVyPUOuPFE5PzVizszN MV;TRW5ITVJ?
PANC-03-27 NXK1eJBqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2j5SWlEPTB;Mz64NVkyPiEQvF2= NEHyelZUSU6JRWK=
NCI-H2228 NUPjRVl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLuSo5WUUN3ME2zMlg{OTJ4IN88US=> MVnTRW5ITVJ?
CAPAN-1 M4PqXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PlfWlEPTB;Mz64O|AxQSEQvF2= NFPDT|dUSU6JRWK=
KLE M{fO[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTNwOUC2O{DPxE1? NGLy[2xUSU6JRWK=
Becker MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVj1dGxYUUN3ME2zMlkzQDR7IN88US=> MojDV2FPT0WU
SK-PN-DW NVLVTVlkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DYbmlEPTB;ND6wO|k4PCEQvF2= MYHTRW5ITVJ?
Hs-578-T M2DXVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\MT2xlUUN3ME20MlA5OzB4IN88US=> NV\wW|V[W0GQR1XS
RS4-11 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:yOFFIUUN3ME20MlEyOjV3IN88US=> NHO2bINUSU6JRWK=
D-502MG M2freWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTRwMU[5PVch|ryP NXfaSlB5W0GQR1XS
GI-1 M4\BdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTRwMkiyPVkh|ryP MV\TRW5ITVJ?
NCI-H209 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvzVmlLUUN3ME20MlM{PzhizszN MWfTRW5ITVJ?
SK-OV-3 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTRwNEixOFkh|ryP MlHLV2FPT0WU
LOUCY NHqweGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLKb|dKSzVyPUSuOFg1OzJizszN NUf1OnJlW0GQR1XS
NCI-H69 M{T0[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTRwNUG3PVMh|ryP M1rsb3NCVkeHUh?=
SCC-9 M2DDS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjxTWM2OD12LkWzNVczKM7:TR?= NV;IOVRpW0GQR1XS
NCI-H526 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFewTVhKSzVyPUSuOVkzQTNizszN MnL2V2FPT0WU
NCI-H2170 MnXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTRwNk[3O|Eh|ryP Mo\tV2FPT0WU
SR NEDseFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXm0dFNTUUN3ME20MlcxODR6IN88US=> M1vDcnNCVkeHUh?=
CMK Mnj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfDTWM2OD12LkeyNVAyKM7:TR?= M2PMcXNCVkeHUh?=
COLO-829 M3fRXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTRwN{ewOFMh|ryP NXnSdXY2W0GQR1XS
HuO-3N1 NX;ZNI5yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnmyTWM2OD12LkizNlgyKM7:TR?= MkjtV2FPT0WU
5637 NHO0OohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTRwOUC0PFch|ryP NYL4PWRSW0GQR1XS
A253 MoTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\1NnJOUUN3ME20Mlk4PzB6IN88US=> MUnTRW5ITVJ?
NB10 MmXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfoPVA{UUN3ME21MlA4PDl5IN88US=> NG\OTXJUSU6JRWK=
SF539 NYTvVGE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTVwMEm5OVkh|ryP NYn4OYVUW0GQR1XS
HAL-01 M3H5bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLqTWM2OD13LkG0OlQ2KM7:TR?= NH\zVmdUSU6JRWK=
MHH-ES-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDLenlKSzVyPUWuNVY6OjVizszN M4XXT3NCVkeHUh?=
DEL M3rrdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4D2XGlEPTB;NT6yO|U5OiEQvF2= MWLTRW5ITVJ?
NCI-H1299 M4fzO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\2NZcxUUN3ME21MlM2ODB5IN88US=> MkHDV2FPT0WU
ChaGo-K-1 MoHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTVwM{WxNFch|ryP NXO3Rpl[W0GQR1XS
HMV-II NIHySpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjJTWM2OD13LkO5OFMh|ryP NHrmTIVUSU6JRWK=
NB69 NXLYeYo1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\GUmlEPTB;NT6zPVY{OSEQvF2= NHf4UJlUSU6JRWK=
NCI-H1882 NW\oZYFCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITFRVVKSzVyPUWuOFM{PjRizszN MVfTRW5ITVJ?
SNU-475 NHPWcnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVryTHJyUUN3ME21MlQ4OTdzIN88US=> M3zNWXNCVkeHUh?=
RXF393 NETWNnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\sSnFCUUN3ME21MlQ5PDl2IN88US=> NXjHcpJWW0GQR1XS
CAL-51 MnPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjhTWM2OD13Lk[yPFU{KM7:TR?= NYnq[FA1W0GQR1XS
SW1573 NX7UPHNmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zhdmlEPTB;NT62N|U{PCEQvF2= NU\hSYhyW0GQR1XS
GCT MmjUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfH[2g2UUN3ME21Mlc2QTJ7IN88US=> MoHvV2FPT0WU
COR-L88 NH3NWXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\RSmlEPTB;NT63Olg1PiEQvF2= NFnJZZdUSU6JRWK=
MDA-MB-134-VI NWjmWlNnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MluwTWM2OD13Lki3OVM3KM7:TR?= Mn;UV2FPT0WU
NB5 MnXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvHPHhKSzVyPUWuPVI1PCEQvF2= M1i0R3NCVkeHUh?=
GMS-10 NIfubXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTVwOU[4OlEh|ryP Ml7OV2FPT0WU
CHP-212 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLEXo9KSzVyPU[uOFgzPjdizszN M{\mW3NCVkeHUh?=
TE-11 NGX0SWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrXTWM2OD14LkW1NVgzKM7:TR?= MofYV2FPT0WU
SW962 NIT2XWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\ZTWM2OD14Lk[1O|c1KM7:TR?= M4XpSnNCVkeHUh?=
MZ2-MEL M2HNVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\yTWM2OD14LkiyNFEyKM7:TR?= M2e1PHNCVkeHUh?=
C-33-A MlvsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\yU4pKSzVyPU[uPVE3QDhizszN MUHTRW5ITVJ?
IGR-1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTZwOUe4NlYh|ryP NIjvUlBUSU6JRWK=
MOLT-4 NUTzXFJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nCc2lEPTB;Nz6xOVQxPiEQvF2= NUPVRnE5W0GQR1XS
NCI-H1650 NWfFSJE1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXznXmtVUUN3ME23MlI4ODR4IN88US=> NYK5WVBVW0GQR1XS
AsPC-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\UOm5[UUN3ME23MlI5PjB3IN88US=> NFf6eZpUSU6JRWK=
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LU-165 NYTsdVlYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTJ{LkW1OEDPxE1? NFT5VXJUSU6JRWK=
COLO-792 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXOTWM2OD1{Mj63NVEyKM7:TR?= NGSxbpdUSU6JRWK=
CAL-62 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLvTWM2OD1{Mj63OVE4KM7:TR?= NWjzeWRkW0GQR1XS
HCE-T NWG1Vlc1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{TXe2lEPTB;MkKuPFYxPiEQvF2= NWjURVM1W0GQR1XS
BB65-RCC Mn;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvqU2JKSzVyPUKzMlA{PDRizszN MlX2V2FPT0WU
SiHa MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3vTWM2OD1{Mz6zPVY5KM7:TR?= MnKyV2FPT0WU
NCI-H2081 Ml2yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;KXFFKSzVyPUK0MlA3PDFizszN NYfxcIM4W0GQR1XS
U251 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnOfWUzUUN3ME2yOE4xPjZ6IN88US=> MoHFV2FPT0WU
LoVo Mn\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUSxTJFvUUN3ME2yOE46Ojh{IN88US=> MoT5V2FPT0WU
GCIY MnPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfEU2JKSzVyPUK1MlI5PzdizszN MWnTRW5ITVJ?
NUGC-3 Mlf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnXXIdCUUN3ME2yOU4{OzF4IN88US=> NUnN[GlzW0GQR1XS
LNCaP-Clone-FGC M1W1UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;rc4MyUUN3ME2yOU4{PDl6IN88US=> NF:yTWNUSU6JRWK=
PA-1 NIHwS|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELpWG9KSzVyPUK1MlY5OzNizszN MVvTRW5ITVJ?
OE33 NYDTe4Z5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\OTWM2OD1{NT62PFU{KM7:TR?= MYnTRW5ITVJ?
HT-29 NHHDXmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zUemlEPTB;MkWuO|g4QSEQvF2= MmTHV2FPT0WU
RCM-1 M2LLVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nMXmlEPTB;MkWuPVY2PiEQvF2= MX;TRW5ITVJ?
NCI-H1522 NGW4[XVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn20TWM2OD1{NT65PVEyKM7:TR?= MnO3V2FPT0WU
LB373-MEL-D NImxdlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnNV4lKSzVyPUK2MlE4ODhizszN NUjL[mhwW0GQR1XS
NCI-H1436 M3znfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1KwN2lEPTB;Mk[uNlc{QCEQvF2= NIq1UJZUSU6JRWK=
COLO-824 NITiboxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLuTWM2OD1{Nj60NFAzKM7:TR?= MXPTRW5ITVJ?
CP50-MEL-B NGLNVnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLpS29QUUN3ME2yOk42Pjd3IN88US=> M3;Q[nNCVkeHUh?=
EGI-1 NWTreHVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrEcXp{UUN3ME2yO{4zPjl6IN88US=> M1fa[XNCVkeHUh?=
LAN-6 MmLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LL[WlEPTB;MkeuN|U6QCEQvF2= MoTTV2FPT0WU
GAK NIrxbWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWq1VVJWUUN3ME2yO{41OjJ3IN88US=> NWHKSldbW0GQR1XS
MDA-MB-175-VII M4fzcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPrPWpKSzVyPUK3MlUxPTNizszN NVXvS5o4W0GQR1XS
JVM-2 NHvlVWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;sVYRKSzVyPUK3MlY3OjJizszN MXXTRW5ITVJ?
UACC-257 MlfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjTTWM2OD1{Nz62PFc5KM7:TR?= NVi1UJo4W0GQR1XS
SW1783 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTCTWM2OD1{Nz63O|g{KM7:TR?= MnnMV2FPT0WU
HT-3 M3LwcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfkTWM2OD1{OD6yNVY3KM7:TR?= MlHKV2FPT0WU
RL MnjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PMTGlEPTB;MkiuOVkyOSEQvF2= M4\Yc3NCVkeHUh?=
TK10 MlfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTJ6Lk[1N|Uh|ryP NHnMZnNUSU6JRWK=
HT55 NHjlSVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlKxTWM2OD1{OT6wOVY2KM7:TR?= M1XK[3NCVkeHUh?=
BT-20 MkPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfPTWM2OD1{OT6wOVg3KM7:TR?= NY\WdoIxW0GQR1XS
TGBC24TKB NIjMSGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTJ7LkG4N|Eh|ryP NITt[lBUSU6JRWK=
HSC-2 M{Xrcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrzTWM2OD1{OT6zNFAyKM7:TR?= NVnYUJVZW0GQR1XS
COLO-205 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorhTWM2OD1{OT6zPVQ1KM7:TR?= MYXTRW5ITVJ?
GT3TKB M{iyVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLKTWM2OD1{OT62PFc4KM7:TR?= Mo\BV2FPT0WU
T47D Mn63S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojWTWM2OD1{OT64OVgzKM7:TR?= NFrncYdUSU6JRWK=
OMC-1 NIO4SW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjRdHhKSzVyPUOwMlE6OTlizszN Mn33V2FPT0WU
PFSK-1 M4PHRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\OTWM2OD1|MT6yOlU6KM7:TR?= MUHTRW5ITVJ?
JEG-3 NV\aR5hwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LZVmlEPTB;M{GuN|U3OSEQvF2= MmToV2FPT0WU
RD NGT2dJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTIXVVKSzVyPUOxMlUyOTVizszN NFqzOVJUSU6JRWK=
NCI-H64 NXe2OZlMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkmyTWM2OD1|Mj60NVc6KM7:TR?= NV\UPFVsW0GQR1XS
DMS-114 NXvUTlZCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TxcWlEPTB;M{KuOFE5OiEQvF2= MVTTRW5ITVJ?
NCI-H661 M33qfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTN{Lk[1NFEh|ryP MWDTRW5ITVJ?
CAL-148 MmLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{S5S2lEPTB;M{KuOlY3PSEQvF2= M1;LeHNCVkeHUh?=
HuP-T3 NVnOPIo6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPUOoFuUUN3ME2zNk46OzN6IN88US=> NXXMZ3pMW0GQR1XS
ES4 NInX[nVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTN|LkG3NFUh|ryP NXXMXnZyW0GQR1XS
Ca9-22 NGHBTppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnUTWM2OD1|Mz60OlAyKM7:TR?= NYPxTJExW0GQR1XS
LP-1 Mk\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTN2LkC5JO69VQ>? M1;oRnNCVkeHUh?=
AU565 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrKNllSUUN3ME2zOE42ODZ7IN88US=> M2fJd3NCVkeHUh?=
MDA-MB-453 NFnXbpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXf2RY12UUN3ME2zOU4yPDJ4IN88US=> NIDqfpJUSU6JRWK=
SNU-C1 NWO1NldIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDqVHdKSzVyPUO1MlE4OSEQvF2= MWPTRW5ITVJ?
SW13 M4PjSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvpTWM2OD1|NT60NFc3KM7:TR?= MkTTV2FPT0WU
NCI-H2122 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPZUZhyUUN3ME2zOU42PTl6IN88US=> NHi3dlVUSU6JRWK=
NCI-H1395 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXnTWM2OD1|NT62OlkyKM7:TR?= M1izOnNCVkeHUh?=
J-RT3-T3-5 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3[0VmlEPTB;M{[uNFI6PCEQvF2= NHv1fndUSU6JRWK=
COR-L279 Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{iwPWlEPTB;M{[uNVk5OSEQvF2= MYTTRW5ITVJ?
ATN-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;QT5NKSzVyPUO2Mlg2OTZizszN NWXSflRQW0GQR1XS
HCC2998 NILSRplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjHOWZKSzVyPUO2Mlg2PzFizszN MmPnV2FPT0WU
UM-UC-3 MnPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTUbZh{UUN3ME2zOk45Pjd3IN88US=> NIjGUWpUSU6JRWK=
DV-90 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTN4LkmwOlch|ryP MoPzV2FPT0WU
ES8 M1\PTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrxTWM2OD1|Nj65N|E5KM7:TR?= MVTTRW5ITVJ?
YAPC NIXEeYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M120bmlEPTB;M{[uPVY2PCEQvF2= NYH3bpNJW0GQR1XS
HCC1599 Mk\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TpZmlEPTB;M{euNVYyQCEQvF2= NILvVG1USU6JRWK=
UMC-11 M3exUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;LR2lEPTB;M{euNlUxOyEQvF2= MnnqV2FPT0WU
THP-1 NWX5[pJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTN5LkK4Olgh|ryP MXfTRW5ITVJ?
OCUB-M MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DVU2lEPTB;M{euNlg6OSEQvF2= NUe2ZXFTW0GQR1XS
NCI-H1693 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3G1ZmlEPTB;M{euN|I{OyEQvF2= M1\MSnNCVkeHUh?=
NCI-H345 MoD4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTN5LkO2NlQh|ryP MX7TRW5ITVJ?
COLO-800 NV7jO2JQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILHPGVKSzVyPUO3MlU3PyEQvF2= MV3TRW5ITVJ?
COLO-320-HSR NWXhTlRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTN6LkO0PVIh|ryP MVzTRW5ITVJ?
NCI-H596 NEfKUmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TxdmlEPTB;M{iuOFQ5QSEQvF2= MoWyV2FPT0WU
NCI-H187 M{TEVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTN6LkezOFQh|ryP MXrTRW5ITVJ?
ECC4 MlXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml31TWM2OD1|OT6wPFE1KM7:TR?= MmLWV2FPT0WU
KP-4 M4L3ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPJ[HZKSzVyPUO5MlEzOzVizszN MlnVV2FPT0WU
NCI-H2227 MlKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDOc|REUUN3ME2zPU4{QTR7IN88US=> NYjYbGZVW0GQR1XS
22RV1 M{jiWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PTfWlEPTB;M{muPFM6QCEQvF2= NWTtVJQ4W0GQR1XS
SAS MlrTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHyZYxKSzVyPUSwMlA6PiEQvF2= NFfPWVRUSU6JRWK=
PANC-10-05 MoW3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTRyLkG4Nlgh|ryP MYXTRW5ITVJ?
MDA-MB-468 M1uzUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIi0[lRKSzVyPUSwMlU{PDZizszN NVz2fFRqW0GQR1XS
RPMI-7951 MmfJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTUNJczUUN3ME20NU4zODd3IN88US=> NXvCTZBRW0GQR1XS
BB49-HNC MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnDTWM2OD12MT6yNVc6KM7:TR?= MYnTRW5ITVJ?
23132-87 M3T4[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrGTWM2OD12MT6yO|MzKM7:TR?= NV63c4dnW0GQR1XS
HCC1569 MkLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGKySHdKSzVyPUSxMlI5QDVizszN M{ezd3NCVkeHUh?=
CP66-MEL NWqw[pIyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzMTWM2OD12Mj6xPFg3KM7:TR?= M{fj[XNCVkeHUh?=
K-562 M4fIXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml6yTWM2OD12Mj6yPFU1KM7:TR?= MnvSV2FPT0WU
OCI-AML2 NEDIbo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXPNpRKSzVyPUSyMlg6PzRizszN M3\0bXNCVkeHUh?=
SW756 MlzLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\STWM2OD12Mz6wN|g5KM7:TR?= MUnTRW5ITVJ?
M14 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fFUGlEPTB;NEOuN|E5QSEQvF2= MnTsV2FPT0WU
NCI-H1623 Ml\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LlNGlEPTB;NEOuOFY1KM7:TR?= M{TmWHNCVkeHUh?=
KNS-81-FD Ml7VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HBNGlEPTB;NEOuO|YzKM7:TR?= NWnKemNqW0GQR1XS
TGBC1TKB NX7rdFhNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\Od5ZKSzVyPUSzMlkxQTlizszN MlL5V2FPT0WU
RERF-LC-MS NYHpfm01T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTnTYs5UUN3ME20N{46OjhzIN88US=> NE\MRpJUSU6JRWK=
PC-3 M2GwVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjFcJdJUUN3ME20OE41OzR5IN88US=> MoDZV2FPT0WU
HuP-T4 MkD6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfUW2tKSzVyPUS0MlQ1PzRizszN M2K0TXNCVkeHUh?=
NCI-H510A NWjoW2ZST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTR2LkewPEDPxE1? M3jCXnNCVkeHUh?=
LB2518-MEL NH:wVnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGX0RpVKSzVyPUS0Mlc6OzdizszN NWfoTmM1W0GQR1XS
TE-5 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2r1WWlEPTB;NEWuPVU5OSEQvF2= NFnGeZNUSU6JRWK=
EW-24 MljZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXjO4ZMUUN3ME20Ok4yPzJ|IN88US=> MXvTRW5ITVJ?
LB647-SCLC NHLpe3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn73TWM2OD12Nj6yNVU2KM7:TR?= MlTQV2FPT0WU
NCI-H524 NH7KZphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnGVnpKSzVyPUS2MlcyQDVizszN NYjWT2tCW0GQR1XS
EW-11 NVrBRlZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvGOpBKSzVyPUS2Mlk{PzNizszN NEOx[5hUSU6JRWK=
KALS-1 NV7idoJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTR5LkKwOlYh|ryP MXTTRW5ITVJ?
BT-474 NGX3ZY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTR5LkSyNVYh|ryP NFzPVphUSU6JRWK=
SK-N-AS NV;2S5ZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnZfJR1UUN3ME20O{45QDhizszN MkHnV2FPT0WU
HCC2157 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTR6LkC3OkDPxE1? NVzDfFZoW0GQR1XS
HCC1954 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTR6LkG3NFQh|ryP MkPhV2FPT0WU
FADU NVTKVZFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jCNGlEPTB;NEiuN|A{OSEQvF2= NWS3VGQ3W0GQR1XS
S-117 MkTKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LDdmlEPTB;NEiuN|gyPSEQvF2= NH;aSVRUSU6JRWK=
LS-1034 NYXtU5NJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTkc2p3UUN3ME20PE44ODZ5IN88US=> MnS0V2FPT0WU
CAMA-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTVTWM2OD12OT61OVQ{KM7:TR?= MV;TRW5ITVJ?
LU-134-A MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zrc2lEPTB;NEmuPFA5PSEQvF2= Mm\yV2FPT0WU
NCI-H1838 NVrvRZJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTR7LkiwPFch|ryP M3rjbHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo In a mouse xenograft model of Ba/F3 cells expressing native Bcr-Abl, AP24534 (2.5 mg/kg and 5 mg/kg) prolongs mice median survival. In the xenograft model of Ba/F3 Bcr-AblT315I, AP24534 (10 mg/kg–50 mg/kg) significantly suppresses tumor growth. AP24534 (30 mg/kg) decreases the phosphorylated Bcr-Abl and phosphorylated CrkL levels in the tumors. [2]

Protocol

Kinase Assay:[1]
+ Expand

Peptide substrate phosphorylation assays with GST-Abl kinase domains:

The effect of AP24534 (0-320 nM) on GST-Abl kinase activity is assessed by using a synthetic peptide substrate (Abltide: EAIYAAPFAKKK). Assays are carried out at 30 °C for 15 min in 25 μL reaction mixture: 8 mM MOPS (pH 7), 0.2 mM EDTA, 50 μM Abltide, 30 mM MgCl2, 10 mM β-glycerol phosphate, 1 mM EGTA, 0.002% Brij-35, 0.4 mM DTT, 0.2 mg/mL BSA, 0.4 mM sodium orthovanadate, 10 nM WT or mutant GST-Abl kinase, and 100 µM ATP/γ-32[P]ATP (5000 cpm/pmol). Reactions are terminated by transferring a portion of the reaction mixture onto a p81 phosphocellulose filter and immersing in 0.75% phosphoric acid. Filters are washed 3 times in 0.75% phosphoric acid, rinsed in acetone, and air dried; phosphate incorporation is determined by scintillation counting. All results are corrected for background binding to the filters, as determined by omitting peptide substrate from the kinase reaction. Time course experiments to establish the linear range of enzymatic activity precedes kinase assays.
Cell Research:[2]
+ Expand
  • Cell lines: Ba/F3 cells expressing Bcr-Abl or a range of single mutations in the kinase domain of Bcr-Abl
  • Concentrations: 0-625 nM
  • Incubation Time: 72 hours
  • Method: Ba/F3 cell lines are distributed in 96-well plates (4 × 103 cells/well) and incubated with AP24534 for 72 hours. Proliferation is measured using a methanethiosulfonate (MTS)-based viability assay (CellTiter96 Aqueous One Solution). All values are normalized to the control wells with no drug. IC50 values are reported as the mean of three independent experiments performed in quadruplicat
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Mouse xenograft models of Ba/F3 cells expressing Bcr-Abl or Bcr-AblT315I
  • Formulation: Dissolved in 25 mM citrate buffer, pH 2.75
  • Dosages: 2.5 mg/kg and 5 mg/kg for Ba/F3 Bcr-Abl; 2.5 mg/kg–50 mg/kg for Ba/F3 Bcr-AblT315I
  • Administration: Oral gavage once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 30 mg/mL (56.33 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 532.56
Formula

C29H27F3N6O

CAS No. 943319-70-8
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02829840 Not yet recruiting Leukemia|FLT3-Mutated Acute Myeloid Leukemia|FLT3-Mutated High-Risk Myelodysplastic Syndrome M.D. Anderson Cancer Center|Ariad Pharmaceuticals September 2016 Phase 1|Phase 2
NCT02398825 Recruiting Chronic Myeloid Leukemia|Chronic Phase|Adults Gruppo Italiano Malattie EMatologiche dellAdulto August 2016 Phase 2
NCT02776605 Recruiting ALL PETHEMA Foundation June 2016 Phase 2
NCT01888562 Withdrawn Endometrial Neoplasms Washington University School of Medicine January 2016 --
NCT02627677 Recruiting Chronic Phase Chronic Myeloid Leukemia Ariad Pharmaceuticals December 2015 Phase 3
NCT02448095 Recruiting Chronic Myeloid Leukemia|Philadelphia Positive Gruppo Italiano Malattie EMatologiche dellAdulto December 2015 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Is the pre-solubilized inhibitor suitable for in vivo whole animal studies? Whether the solvent contains any non-DMSO stabilizer?

  • Answer:

    Our 10mM Ponatinib in DMSO solution is in pure DMSO and there is not other stabilizer added. You can use it for animal study. The vehicle we suggest for Ponatinib is: 30% PEG400/0.5% Tween80/5% Propylene glycol. It is a milky suspension used for oral gavage.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID