Sulforaphane

Catalog No.S5771 Batch:S577103

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Technical Data

Formula

C6H11NOS2
Molecular Weight 177.29 CAS No. 4478-93-7
Solubility (25°C)* In vitro Water 50 mg/mL (282.02 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.,
Targets
Nrf2 [1] HDAC [1] NF-κB [1] ROS [1] HO-1 [1]
In vitro

Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that CC90003 induces autophagy by activating ROS/p53.[3]
In vivo

Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.[4]
Density 1.17 g/mL

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    Human CRC cell HCT-116 and SW620

  • Concentrations

    20 μM

  • Incubation Time

    24 h

  • Method

    Cells are cultured in a 96-well microplate at a density of 5×103 cells/well for 24 h. Then, the cells are divided into several groups and treated with different conditions. Cell viability is assessed with CCK-8 after treating drugs. The absorbance value at 450 nm is read with a 96-well plate reader. Nuclear staining with Hoechst33342 is used to analyze the apoptosis. For colony formation assays, an equal number of cells after different treatments are planted in six-well plates.Colonies are visualized by crystal violet staining 14 days after plating.
Animal Study:

[4]
  • Animal Models

    diet-induced obese mice

  • Dosages

    25 μl

  • Administration

    i.p.

Selleck's Sulforaphane has been cited by 15 publications

Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220
Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220
Identifying autophagy-related genes as potential targets for immunotherapy in tuberculosis [ Int Immunopharmacol, 2023, 118:109956] PubMed: 36931175
Variable p53/Nrf2 crosstalk contributes to triptolide-induced hepatotoxic process [ Toxicol Lett, 2023, 379:67-75] PubMed: 36990132
SARS-CoV-2 virus NSP14 Impairs NRF2/HMOX1 activation by targeting Sirtuin 1 [ Cell Mol Immunol, 2022, 19(8):872-882] PubMed: 35732914
Sulforaphane reduces obesity by reversing leptin resistance [ Elife, 2022, 11e67368] PubMed: 35323110
Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages [ Int J Mol Sci, 2022, 23(15)8603] PubMed: 35955737
A Gene Co-Expression Network-Based Drug Repositioning Approach Identifies Candidates for Treatment of Hepatocellular Carcinoma [ Cancers (Basel), 2022, 14(6)1573] PubMed: 35326724
Cystic fibrosis patient-derived bronchial organoids unveil druggable pathways againstMycobacterium abscessus infection [ bioRxiv, 2022, 10.1101/2022.01.03.474765] PubMed: none
Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2 [ Cell Commun Signal, 2021, 19(1):35] PubMed: 33736642

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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