Home Metabolism phosphatase inhibitor SPI-112 For research use only.

SPI-112

SPI-112 is a not cell permeable inhibitor of Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) with IC50 of 1 μM in cell-free assay.

SPI-112 Chemical Structure

SPI-112 Chemical Structure

CAS: 1051387-90-6

Purity & Quality Control

Batch: S024401 DMSO] 94 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.68%
99.68

SPI-112 Related Products

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Biological Activity

Description SPI-112 is a not cell permeable inhibitor of Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) with IC50 of 1 μM in cell-free assay.
Targets
SHP-2 [1]
(Cell-free assay)
1 μM
In vitro
In vitro

SPI-112 is a not cell permeable inhibitor of Src homology region 2 domain-containing phosphatase 2 (SHP-2, PTPN11) with IC50 of 1.0 μM, suggesting that SPI-112 binds to the catalytic pocket.[1]
Cell Research Cell lines MDA-MB-468 breast cancer cells
Concentrations 100 μM
Incubation Time 10 min
Method

Sub-confluent MDA-MB-468 cells were serum-starved in DMEM/0.1% BSA for 18 h, pretreated overnight with SPI-112 (100 μM) and stimulated with EGF (50 ng/ml, 10 min) or mocked treated as indicated. Shp2 was immunoprecipitated from cell lysate supernatants and Shp2 PTP activity was measured.

Chemical Information & Solubility

Molecular Weight 468.46 Formula

C22H17FN4O5S
CAS No. 1051387-90-6 SDF --
Smiles C1=CC(=CC(=C1)N=NC2=C(NC3=C2C=C(C=C3)S(=O)(=O)NCC4=CC=C(C=C4)F)O)C(=O)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 94 mg/mL ( (200.65 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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