Sophoricoside

Catalog No.S3776 Batch:S377601

Technical Data

Formula	C₂₁H₂₀O₁₀
Molecular Weight	432.38	CAS No.	152-95-4
Solubility (25°C)*	In vitro	DMSO	86 mg/mL (198.89 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae) with numerous pharmacological effects, including anti- inflammatory, anti-cancer and immunosuppressive effects.
In vitro	Treatment with sophoricoside at concentrations of 1–10 μM inhibited lipid accumulation in HepG2 cells in a dose-dependent manner. At the same concentration range, no effect on cell viability was observed in the MTT assay. Inhibition of lipogenesis was associated with the downregulation of SREBP-1a, SREBP-1c, SREBP-2 and their downstream target genes (FAS, ACC, HMGR). Sophoricoside has the capability to increase glucose uptake by C2C12 myotubes. It also effectively inhibited the activities of α-glucosidase and α-amylase in vitro^[1].
In vivo	Sophoricoside remarkably lowered postprandial hyperglycaemia in starch-loaded C57BL6/J mice^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HepG2 Cells Concentrations 0-75 μM Incubation Time 48 h Method HepG2 cells were cultured in a 24-well plate. After reaching confluence, the cells were incubated for 48 h in the presence of sophoricoside. Subsequently, the culture medium was removed and replaced by 500 μL of fresh culture medium containing 10% sterile filtered MTT. After 3 h, the insoluble formazan crystals were dissolved in 500 μL/well isopropanol and absorbance was measured at 570 nm, using the 630 nm reading as a reference.
Animal Study:^{^[1]}	Animal Models C57BL6/J mice Dosages 50, 100 and 200 mg/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
HepG2 Cells
Concentrations
0-75 μM
Incubation Time
48 h
Method
HepG2 cells were cultured in a 24-well plate. After reaching confluence, the cells were incubated for 48 h in the presence of sophoricoside. Subsequently, the culture medium was removed and replaced by 500 μL of fresh culture medium containing 10% sterile filtered MTT. After 3 h, the insoluble formazan crystals were dissolved in 500 μL/well isopropanol and absorbance was measured at 570 nm, using the 630 nm reading as a reference.

Animal Study:^{^[1]}

Animal Models
C57BL6/J mice
Dosages
50, 100 and 200 mg/kg
Administration
oral

References

[1] Wu C, et al. Molecules. 2013, 18(12):15624-35.

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