SMER28

Catalog No.S8240 Batch:S824002

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Technical Data

Formula

C11H10BrN3
Molecular Weight 264.12 CAS No. 307538-42-7
Solubility (25°C)* In vitro DMSO 53 mg/mL (200.66 mM)
Ethanol 53 mg/mL (200.66 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.
Targets
Autophagy [1]
In vitro SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures

  • Concentrations

    10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures

  • Incubation Time

    16hrs

  • Method

    MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10 μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.

Selleck's SMER28 has been cited by 5 publications

Drug antagonism and single-agent dominance result from differences in death kinetics. [ Nat Chem Biol, 2020, 6] PubMed: 32251407
Drug GRADE: An Integrated Analysis of Population Growth and Cell Death Reveals Drug-Specific and Cancer Subtype-Specific Response Profiles [ Cell Rep, 2020, 31(12):107800] PubMed: 32579927
Stimulation of Hair Growth by Small Molecules that Activate Autophagy [ Cell Rep, 2019, 27(12):3413-3421] PubMed: 31216464
Trehalose Modulates Autophagy Process to Counteract Gliadin Cytotoxicity in an In Vitro Celiac Disease Model. [ Cells, 2019, 8(4)] PubMed: 31013754
Novel cell-and tissue-based assays for detecting misfolded and aggregated protein accumulation within aggresomes and inclusion bodies [Shen D Cell Biochem Biophys, 2011, 60(3):173-85] PubMed: 21132543

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.