Sesamol

Catalog No.S3626 Synonyms: 1,3-Benzodioxol-5-ol, 3,4-Methylenedioxyphenol

For research use only.

Sesamol (1,3-Benzodioxol-5-ol, 3,4-Methylenedioxyphenol), a natural organic compound, is regarded as a major antioxidant component in the oil with chemoprevention, antimutagenic, and antihepatotoxic activities. It induces apoptosis of cancer and cardiovascular cells.

Sesamol Chemical Structure

CAS No. 533-31-3

Purity & Quality Control

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Biological Activity

Description Sesamol (1,3-Benzodioxol-5-ol, 3,4-Methylenedioxyphenol), a natural organic compound, is regarded as a major antioxidant component in the oil with chemoprevention, antimutagenic, and antihepatotoxic activities. It induces apoptosis of cancer and cardiovascular cells.
In vitro

Sesamol treatment suppresses colony formation, elicits S phase arrest during cell cycle progression, and induces both intrinsic and extrinsic apoptotic pathway in vitro with a dose-dependent manner. Furthermore, sesamol treatment elicits mitochondrial dysfunction by inducing a loss of mitochondrial membrane potential. Impaired mitochondria and accumulated H2O2 production results in disturbance of redox-sensitive signaling including Akt and MAPKs pathways. Mitochondrial biogenesis is inhibited as suggested by the decline in expression of mitochondrial complex I subunit ND1, and the upstream AMPK/PGC1α signals. Importantly, sesamol inhibits mitophagy and autophagy through impeding the PI3K Class III/Belin-1 pathway. Autophagy stimulator rapamycin reverses sesamol-induced apoptosis and mitochondrial respiration disorders[1]. Sesamol increases the activity of caspase 8, 9, and 3/7, indicating that apoptotic cell death occurred through both extrinsic and intrinsic pathways. Sesamol causes the loss of mitochondrial transmembrane potential signifying intrinsic apoptosis induction in human lung adenocarcinoma (SK-LU-1) cells[2].

In vivo Sesamol has potent anti-hepatoma activity in a xenograft nude mice model[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HepG2 cells, BRL-3A cells
  • Concentrations: 0, 0.01, 0.05, 0.1, 0.2, 0.5 or 1 mM
  • Incubation Time: 24 h
  • Method: The cytotoxicity of sesamol on HepG2 cells and BRL-3A cells is examined by using MTT assay. Briefly, cells in exponential growth are seeded into 96-well plates at a density of 1 × 105 cells/mL. The cells are then treated with sesamol at various doses (0, 0.01, 0.05, 0.1, 0.2, 0.5 or 1 mM). After 24 h, the supernatant is discarded and 100 μL FBS free medium is added. 100 μL of MTT is added in each well, and cells are further incubated for 4 h in the dark. After incubation, the medium is discarded, 100 μL of DMSO is added, and the optical density of each well is measured at 570 nm.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: Balb/c nu/nu mice
  • Dosages: 100 mg/kg or 200 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 27 mg/mL
(195.48 mM)
Water 27 mg/mL
(195.48 mM)
Ethanol 27 mg/mL
(195.48 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 138.12
Formula

C7H6O3

CAS No. 533-31-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1OC2=C(O1)C=C(C=C2)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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