CAS No. 951441-04-6
Batch:
Purity:99.83
Chemical Structure
Check the
Sephin-1
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 2-[(E)-(2-chlorophenyl)methylideneamino]guanidine | |
|---|---|---|
| CAS Number | 951441-04-6 | |
| Molecular Formula |
C8H9ClN4 |
|
| Molecular Weight (MW) | 196.64 | |
| SMILES | NC(=N)N\N=C\C1=CC=CC=C1Cl | |
| InChIKey | PDWJALXSRRSUHR-LFYBBSHMSA-N |
Sephin-1 (NSC-65390) is a selective inhibitor of PPP1R15A (GADD34), a regulatory subunit of protein phosphatase 1. By inhibiting PPP1R15A-mediated protein dephosphorylation, Sephin-1 prolongs the phosphorylation of eukaryotic translation initiation factor 2 alpha (eIF2α) during endoplasmic reticulum stress. This attenuation enhances the integrated stress response pathway, preventing proteostasis collapse and displaying neuroprotective activity in cellular and animal models of protein misfolding diseases. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
39 mg/mL
(198.33 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 39 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.